Restriction map for
pFN21AB5025
(Linear) (Six-base) MAPSORT of: KIBB5025.seq Check: 4785 from: 1 to: 717
KIBB5025.orf
With 182 enzymes: SgfI *
July 18, 2007 13:37 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 364 412 717
Size: 364 48 305
Fragments arranged by size:
364 305 48
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 277 309 717
Size: 277 32 408
Fragments arranged by size:
408 277 32
ApaI G_GGCC'C
Cuts at: 0 372 717
Size: 372 345
AvaI C'yCGr_G
Cuts at: 0 112 118 717
Size: 112 6 599
Fragments arranged by size:
599 112 6
BanI G'GyrC_C
Cuts at: 0 88 559 717
Size: 88 471 158
Fragments arranged by size:
471 158 88
BanII G_rGCy'C
Cuts at: 0 107 119 372 717
Size: 107 12 253 345
Fragments arranged by size:
345 253 107 12
BbeI G_GCGC'C
Cuts at: 0 92 717
Size: 92 625
BbsI GAAGACnn'nnnn_
Cuts at: 0 222 717
Size: 222 495
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 187 221 717
Size: 187 34 496
Fragments arranged by size:
496 187 34
BclI T'GATC_A
Cuts at: 0 401 717
Size: 401 316
BlpI GC'TnA_GC
Cuts at: 0 163 175 436 632 717
Size: 163 12 261 196 85
Fragments arranged by size:
261 196 163 85 12
Bme1580I G_kGCm'C
Cuts at: 0 372 717
Size: 372 345
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 304 565 717
Size: 49 255 261 152
Fragments arranged by size:
261 255 152 49
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 682 717
Size: 682 35
BsaHI Gr'CG_yC
Cuts at: 0 89 717
Size: 89 628
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 4 34 277 307 717
Size: 4 30 243 30 410
Fragments arranged by size:
410 243 30 30 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 25 64 73 670 673 676 717
Size: 25 39 9 597 3 3 41
Fragments arranged by size:
597 41 39 25 9 3 3
BseYI C'CCAG_C
Cuts at: 0 71 93 170 310 363 717
Size: 71 22 77 140 53 354
Fragments arranged by size:
354 140 77 71 53 22
Bsp1286I G_dGCh'C
Cuts at: 0 107 119 372 717
Size: 107 12 253 345
Fragments arranged by size:
345 253 107 12
BsrBI CCG'CTC
Cuts at: 0 645 717
Size: 645 72
BsrFI r'CCGG_y
Cuts at: 0 35 179 717
Size: 35 144 538
Fragments arranged by size:
538 144 35
BtgI C'CryG_G
Cuts at: 0 140 473 717
Size: 140 333 244
Fragments arranged by size:
333 244 140
ClaI AT'CG_AT
Cuts at: 0 533 717
Size: 533 184
EaeI y'GGCC_r
Cuts at: 0 2 143 602 717
Size: 2 141 459 115
Fragments arranged by size:
459 141 115 2
EarI CTCTTCn'nnn_
Cuts at: 0 388 717
Size: 388 329
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 664 717
Size: 664 53
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 304 364 412 565 717
Size: 49 255 60 48 153 152
Fragments arranged by size:
255 153 152 60 49 48
EcoNI CCTnn'n_nnAGG
Cuts at: 0 274 717
Size: 274 443
EcoO109I rG'GnC_Cy
Cuts at: 0 279 368 369 717
Size: 279 89 1 348
Fragments arranged by size:
348 279 89 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 456 588 717
Size: 456 132 129
Fragments arranged by size:
456 132 129
HaeII r_GCGC'y
Cuts at: 0 36 92 152 170 206 222 514 717
Size: 36 56 60 18 36 16 292 203
Fragments arranged by size:
292 203 60 56 36 36 18 16
HindIII A'AGCT_T
Cuts at: 0 520 717
Size: 520 197
KasI G'GCGC_C
Cuts at: 0 88 717
Size: 88 629
MscI TGG'CCA
Cuts at: 0 4 717
Size: 4 713
MspA1I CmG'CkG
Cuts at: 0 75 174 475 717
Size: 75 99 301 242
Fragments arranged by size:
301 242 99 75
NaeI GCC'GGC
Cuts at: 0 37 181 717
Size: 37 144 536
Fragments arranged by size:
536 144 37
NarI GG'CG_CC
Cuts at: 0 89 717
Size: 89 628
NgoMIV G'CCGG_C
Cuts at: 0 35 179 717
Size: 35 144 538
Fragments arranged by size:
538 144 35
NspI r_CATG'y
Cuts at: 0 294 717
Size: 294 423
PfoI T'CCnGG_A
Cuts at: 0 251 443 717
Size: 251 192 274
Fragments arranged by size:
274 251 192
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 277 309 717
Size: 277 32 408
Fragments arranged by size:
408 277 32
PpuMI rG'GwC_Cy
Cuts at: 0 279 717
Size: 279 438
PspOMI G'GGCC_C
Cuts at: 0 368 717
Size: 368 349
PstI C_TGCA'G
Cuts at: 0 226 491 717
Size: 226 265 226
Fragments arranged by size:
265 226 226
PvuII CAG'CTG
Cuts at: 0 75 174 717
Size: 75 99 543
Fragments arranged by size:
543 99 75
SacII CC_GC'GG
Cuts at: 0 476 717
Size: 476 241
SapI GCTCTTCn'nnn_
Cuts at: 0 388 717
Size: 388 329
SfcI C'TryA_G
Cuts at: 0 222 487 717
Size: 222 265 230
Fragments arranged by size:
265 230 222
SfoI GGC'GCC
Cuts at: 0 90 717
Size: 90 627
SmlI C'TyrA_G
Cuts at: 0 697 717
Size: 697 20
StyI C'CwwG_G
Cuts at: 0 581 717
Size: 581 136
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 4 36 717
Size: 4 32 681
Fragments arranged by size:
681 32 4
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 12 717
Size: 12 705
Enzymes that do cut:
AcuI AloI ApaI AvaI BanI BanII BbeI BbsI
BcgI BclI BlpI Bme1580I BpmI BpuEI BsaHI BsaXI
BseRI BseYI Bsp1286I BsrBI BsrFI BtgI ClaI EaeI
EarI EciI Eco57MI EcoNI EcoO109I FalI HaeII HindIII
KasI MscI MspA1I NaeI NarI NgoMIV NspI PfoI
PpiI PpuMI PspOMI PstI PvuII SacII SapI SfcI
SfoI SmlI StyI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbvCI BciVI BfrBI
BglI BglII BmgBI BmrI BmtI BplI Bpu10I BsaI
BsaAI BsaBI BsaWI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI BspEI BspHI BspMI BsrDI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI
BtsI CspCI DraI DraIII DrdI EagI EcoICRI EcoRI
EcoRV FseI FspI FspAI Hin4I HincII HpaI KpnI
MfeI MluI MmeI MslI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PciI PflMI PmeI PmlI
PshAI PsiI PspXI PsrI PvuI RsrII SacI SalI
SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
TatI Tth111I XbaI XhoI XmaI XmnI ZraI