Restriction map for
pFN21AB5016
(Circular) (Six-base) MAPSORT of: pFN21AB5016.seq Check: 3008 from: 1 to: 6736
pFN21AB5016 6736 bp
With 182 enzymes: SgfI *
February 27, 2009 14:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2208 4546 278
Size: 53 83 186 936 672 2338 2468
Fragments arranged by size:
2468 2338 936 672 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4079 1937
Size: 2142 4594
Acc65I G'GTAC_C
Cuts at: 4057 4057
Size: 6736
AclI AA'CG_TT
Cuts at: 1563 4863 5236 1563
Size: 3300 373 3063
Fragments arranged by size:
3300 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2207 2472 2604 2991 4799 6183 6451 1632
Size: 575 265 132 387 1808 1384 268 1917
Fragments arranged by size:
1917 1808 1384 575 387 268 265 132
AfeI AGC'GCT
Cuts at: 2152 3086 2152
Size: 934 5802
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6539
AflIII A'CryG_T
Cuts at: 1203 5635 6612 1203
Size: 4432 977 1327
Fragments arranged by size:
4432 1327 977
AgeI A'CCGG_T
Cuts at: 5567 5567
Size: 6736
AhdI GACnn_n'nnGTC
Cuts at: 5465 5465
Size: 6736
AleI CACnn'nnGTG
Cuts at: 1166 3342 1166
Size: 2176 4560
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6736
AlwNI CAG_nnn'CTG
Cuts at: 4479 6051 4479
Size: 1572 5164
ApaI G_GGCC'C
Cuts at: 3065 3065
Size: 6736
ApaLI G'TGCA_C
Cuts at: 4793 5949 4793
Size: 1156 5580
ApoI r'AATT_y
Cuts at: 1493 3685 4019 4045 4271 4423 6532 1493
Size: 2192 334 26 226 152 2109 1697
Fragments arranged by size:
2192 2109 1697 334 226 152 26
AseI AT'TA_AT
Cuts at: 160 1032 5290 160
Size: 872 4258 1606
Fragments arranged by size:
4258 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6736
AvaI C'yCGr_G
Cuts at: 1870 1943 3644 4061 1870
Size: 73 1701 417 4545
Fragments arranged by size:
4545 1701 417 73
BamHI G'GATC_C
Cuts at: 4066 4433 4066
Size: 367 6369
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4057 5512 618
Size: 325 217 627 2270 1455 1842
Fragments arranged by size:
2270 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2323 2415 3065 3121 3442 4055 1391
Size: 932 92 650 56 321 613 4072
Fragments arranged by size:
4072 932 650 613 321 92 56
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2195 2468 2693 2891 928
Size: 971 296 273 225 198 4773
Fragments arranged by size:
4773 971 296 273 225 198
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4927 4961 1903
Size: 34 50 34 2906 34 3678
Fragments arranged by size:
3678 2906 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4628 5838 4628
Size: 1210 5526
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6736
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4032 5347 136
Size: 107 122 71 631 605 2360 1315 1525
Fragments arranged by size:
2360 1525 1315 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6731 6731
Size: 6736
BlpI GC'TnA_GC
Cuts at: 4153 4153
Size: 6736
Bme1580I G_kGCm'C
Cuts at: 1790 2391 3065 4797 5953 1790
Size: 601 674 1732 1156 2573
Fragments arranged by size:
2573 1732 1156 674 601
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6736
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2410 3757 4467
Size: 416 53 83 399 24 993 1347 710
Cuts at: 4467 5425 442
Size: 958 1753
Fragments arranged by size:
1753 1347 993 958 710 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6736
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2143 2143
Size: 6736
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2284 3095 3575 5396 1239
Size: 1045 811 480 1821 2579
Fragments arranged by size:
2579 1821 1045 811 480
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2871 3257 4861 5726 6024 2871
Size: 386 1604 865 298 3583
Fragments arranged by size:
3583 1604 865 386 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2259 3459 5399 882
Size: 617 760 1200 1940 2219
Fragments arranged by size:
2219 1940 1200 760 617
BsaAI yAC'GTr
Cuts at: 493 1204 3284 4527 493
Size: 711 2080 1243 2702
Fragments arranged by size:
2702 2080 1243 711
BsaBI GATnn'nnATC
Cuts at: 6730 6730
Size: 6736
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2205 3054 4543 4925
Size: 53 83 186 936 672 849 1489 382
Cuts at: 4925 5560 275
Size: 635 1451
Fragments arranged by size:
1489 1451 936 849 672 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5169 5567 5841 5988 6399 5169
Size: 398 274 147 411 5506
Fragments arranged by size:
5506 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2282 2312 1178
Size: 30 165 30 879 30 5602
Fragments arranged by size:
5602 879 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2345 2513 2516 2519 2522 2651 2741 2878
Size: 1159 168 3 3 3 129 90 137
Cuts at: 2878 2954 3152 3167 3668 3899 1186
Size: 76 198 15 501 231 4023
Fragments arranged by size:
4023 1159 501 231 198 168 137 129
90 76 15 3 3 3
BseYI C'CCAG_C
Cuts at: 2269 3173 5939 6435 2269
Size: 904 2766 496 2570
Fragments arranged by size:
2766 2570 904 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6163
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4186 4947 5975 1540
Size: 275 186 2185 761 1028 2301
Fragments arranged by size:
2301 2185 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3899 4797 4882 5953 3899
Size: 898 85 1071 4682
Fragments arranged by size:
4682 1071 898 85
BsmI GAATG_Cn'
Cuts at: 2674 4247 4340 2674
Size: 1573 93 5070
Fragments arranged by size:
5070 1573 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2323 2391 2415 3065 3121 3442 3899
Size: 399 533 68 24 650 56 321 457
Cuts at: 3899 4055 4797 4882 5953 1391
Size: 156 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 650 533 457 399 321
156 85 68 56 24
BspEI T'CCGG_A
Cuts at: 6399 6399
Size: 6736
BspHI T'CATG_A
Cuts at: 4625 6355 4625
Size: 1730 5006
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3477 4091 844
Size: 2633 614 3489
Fragments arranged by size:
3489 2633 614
BsrBI CCG'CTC
Cuts at: 1394 1823 2405 4623 1394
Size: 429 582 2218 3507
Fragments arranged by size:
3507 2218 582 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3993 5231 5405 65
Size: 1183 2745 1238 174 1396
Fragments arranged by size:
2745 1396 1238 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3889 5380 5567 1700
Size: 110 2079 1491 187 2869
Fragments arranged by size:
2869 2079 1491 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6736
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6736
BssSI C'ACGA_G
Cuts at: 2291 2839 4796 5808 2291
Size: 548 1957 1012 3219
Fragments arranged by size:
3219 1957 1012 548
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6736
BstBI TT'CG_AA
Cuts at: 5540 5540
Size: 6736
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6736
BstXI CCAn_nnnn'nTGG
Cuts at: 2071 2720 3450 2071
Size: 649 730 5357
Fragments arranged by size:
5357 730 649
BstYI r'GATC_y
Cuts at: 1972 4066 4433 4818 4835 6287 6373 6385 6731
Size: 2094 367 385 17 1452 86 12 346
Cuts at: 6731 1972
Size: 1977
Fragments arranged by size:
2094 1977 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3066 3066
Size: 6736
BtgI C'CryG_G
Cuts at: 513 1065 2040 2280 2857 513
Size: 552 975 240 577 4392
Fragments arranged by size:
4392 975 577 552 240
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5997
Fragments arranged by size:
5997 683 56
BtsI GCAGTG_nn'
Cuts at: 2557 4252 5046 5066 2557
Size: 1695 794 20 4227
Fragments arranged by size:
4227 1695 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6736
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3374 3409 529
Size: 35 2810 35 3856
Fragments arranged by size:
3856 2810 35 35
DraI TTT'AAA
Cuts at: 4040 4040
Size: 6736
DraIII CAC_nnn'GTG
Cuts at: 3389 3389
Size: 6736
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2444 3941 5743 809
Size: 507 1128 1497 1802 1802
Fragments arranged by size:
1802 1802 1497 1128 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2646 2958 4183
Size: 54 1425 50 275 18 816 312 1225
Cuts at: 4183 5072 6701 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1225 889 816 312 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4183 1537
Size: 275 2371 4090
Fragments arranged by size:
4090 2371 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2474 2550 2565 2699 3219 3306 3545 3593
Size: 1121 76 15 134 520 87 239 48
Cuts at: 3593 3613 3755 4666 1353
Size: 20 142 911 3423
Fragments arranged by size:
3423 1121 911 520 239 142 134 87
76 48 20 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5323 5697 5843 387
Size: 1064 138 3734 374 146 1280
Fragments arranged by size:
3734 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2207 2284 2472 2604 2991 3095 3575
Size: 393 575 77 188 132 387 104 480
Cuts at: 3575 4799 5396 6183 6451 1239
Size: 1224 597 787 268 1524
Fragments arranged by size:
1524 1224 787 597 575 480 393 387
268 188 132 104 77
EcoNI CCTnn'n_nnAGG
Cuts at: 2302 3950 2302
Size: 1648 5088
EcoO109I rG'GnC_Cy
Cuts at: 2122 2880 3061 3062 3070 3232 3778 2122
Size: 758 181 1 8 162 546 5080
Fragments arranged by size:
5080 758 546 181 162 8 1
EcoRI G'AATT_C
Cuts at: 4045 4423 4045
Size: 378 6358
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3706 3706
Size: 6736
FspI TGC'GCA
Cuts at: 5242 5242
Size: 6736
HaeII r_GCGC'y
Cuts at: 1678 2154 3088 5883 1678
Size: 476 934 2795 2531
Fragments arranged by size:
2795 2531 934 476
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2413 2445 2801 2833 2867
Size: 32 135 32 841 32 356 32 34
Cuts at: 2867 2899 5377 5409 5451 5483 1373
Size: 32 2478 32 42 32 2626
Fragments arranged by size:
2626 2478 841 356 135 42 34 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4080 4326 669
Size: 988 281 2142 246 3079
Fragments arranged by size:
3079 2142 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6736
HpaI GTT'AAC
Cuts at: 4326 4326
Size: 6736
KpnI G_GTAC'C
Cuts at: 4061 4061
Size: 6736
MfeI C'AATT_G
Cuts at: 4335 4335
Size: 6736
MluI A'CGCG_T
Cuts at: 6612 6612
Size: 6736
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2460 5850 6034 1305
Size: 1155 3390 184 2007
Fragments arranged by size:
3390 2007 1155 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2648 2960 10
Size: 54 1425 343 816 312 3786
Fragments arranged by size:
3786 1425 816 343 312 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2069 3204 3342 4694 5053 5212 518
Size: 648 903 1135 138 1352 359 159 2042
Fragments arranged by size:
2042 1352 1135 903 648 359 159 138
MspA1I CmG'CkG
Cuts at: 1606 2273 3539 3857 4152 4829 5977 6222 1606
Size: 667 1266 318 295 677 1148 245 2120
Fragments arranged by size:
2120 1266 1148 677 667 318 295 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6184
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6736
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6736
NotI GC'GGCC_GC
Cuts at: 4183 4183
Size: 6736
NspI r_CATG'y
Cuts at: 1132 1229 2666 4094 5639 1132
Size: 97 1437 1428 1545 2229
Fragments arranged by size:
2229 1545 1437 1428 97
PasI CC'CwG_GG
Cuts at: 1348 2324 3443 1348
Size: 976 1119 4641
Fragments arranged by size:
4641 1119 976
PciI A'CATG_T
Cuts at: 5635 5635
Size: 6736
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6736
PmeI GTTT'AAAC
Cuts at: 4040 4040
Size: 6736
PmlI CAC'GTG
Cuts at: 3284 3284
Size: 6736
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3305 3337 4805 4837 6318 6350 3305
Size: 32 1468 32 1481 32 3691
Fragments arranged by size:
3691 1481 1468 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2122 2880 3070 3778 2122
Size: 758 190 708 5080
Fragments arranged by size:
5080 758 708 190
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6736
PsiI TTA'TAA
Cuts at: 4306 4306
Size: 6736
PspOMI G'GGCC_C
Cuts at: 3061 3061
Size: 6736
PstI C_TGCA'G
Cuts at: 830 3474 4088 830
Size: 2644 614 3478
Fragments arranged by size:
3478 2644 614
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6736
PvuII CAG'CTG
Cuts at: 2273 3539 3857 2273
Size: 1266 318 5152
Fragments arranged by size:
5152 1266 318
SalI G'TCGA_C
Cuts at: 1936 4078 1936
Size: 2142 4594
SanDI GG'GwC_CC
Cuts at: 3778 3778
Size: 6736
SapI GCTCTTCn'nnn_
Cuts at: 2474 2565 3593 2474
Size: 91 1028 5617
Fragments arranged by size:
5617 1028 91
SbfI CC_TGCA'GG
Cuts at: 3474 4088 3474
Size: 614 6122
ScaI AGT'ACT
Cuts at: 4984 6696 4984
Size: 1712 5024
SfcI C'TryA_G
Cuts at: 826 1045 2998 3088 3470 3841 4001 4084 5219
Size: 219 1953 90 382 371 160 83 1135
Cuts at: 5219 5900 6091 826
Size: 681 191 1471
Fragments arranged by size:
1953 1471 1135 681 382 371 219 191
160 90 83
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6736
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6736
SmaI CCC'GGG
Cuts at: 1872 4063 1872
Size: 2191 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2886 3236 3644 4840 5741 6003
Size: 197 926 943 350 408 1196 901 262
Cuts at: 6003 820
Size: 1553
Fragments arranged by size:
1553 1196 943 926 901 408 350 262
197
SnaBI TAC'GTA
Cuts at: 493 4527 493
Size: 4034 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6736
SphI G_CATG'C
Cuts at: 1132 4094 1132
Size: 2962 3774
SspI AAT'ATT
Cuts at: 5 52 4660 5
Size: 47 4608 2081
Fragments arranged by size:
4608 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6736
StyI C'CwwG_G
Cuts at: 513 1065 2341 4175 513
Size: 552 1276 1834 3074
Fragments arranged by size:
3074 1834 1276 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2173 3317 3914 4763 4780 4933 5118
Size: 1255 593 1144 597 849 17 153 185
Cuts at: 5118 5563 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1144 849 597 593 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4982 6694 96
Size: 274 80 33 51 1443 3005 1712 138
Fragments arranged by size:
3005 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2282 2314 3062 3094 6696 6728 2282
Size: 32 748 32 3602 32 2290
Fragments arranged by size:
3602 2290 748 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6736
XbaI T'CTAG_A
Cuts at: 4072 4072
Size: 6736
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2821 3390 2821
Size: 569 6167
XhoI C'TCGA_G
Cuts at: 1943 3644 1943
Size: 1701 5035
XmaI C'CCGG_G
Cuts at: 1870 4061 1870
Size: 2191 4545
XmnI GAAnn'nnTTC
Cuts at: 3320 3686 3689 4865 3320
Size: 366 3 1176 5191
Fragments arranged by size:
5191 1176 366 3
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2206 4544 276
Size: 53 83 186 936 672 2338 2468
Fragments arranged by size:
2468 2338 936 672 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I EcoICRI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PspXI PsrI RsrII SacI SacII SexAI SfiI SfoI
SrfI SwaI