Restriction map for
pFN21AB5016
(Linear) (Six-base) MAPSORT of: KIBB5016.seq Check: 4209 from: 1 to: 2031
KIBB5016.orf
With 182 enzymes: SgfI *
July 18, 2007 13:37 ..
AatII G_ACGT'C
Cuts at: 0 203 2031
Size: 203 1828
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 467 599 986 2031
Size: 202 265 132 387 1045
Fragments arranged by size:
1045 387 265 202 132
AfeI AGC'GCT
Cuts at: 0 147 1081 2031
Size: 147 934 950
Fragments arranged by size:
950 934 147
AleI CACnn'nnGTG
Cuts at: 0 1337 2031
Size: 1337 694
ApaI G_GGCC'C
Cuts at: 0 1060 2031
Size: 1060 971
ApoI r'AATT_y
Cuts at: 0 1680 2014 2031
Size: 1680 334 17
Fragments arranged by size:
1680 334 17
AvaI C'yCGr_G
Cuts at: 0 1639 2031
Size: 1639 392
BanII G_rGCy'C
Cuts at: 0 318 410 1060 1116 1437 2031
Size: 318 92 650 56 321 594
Fragments arranged by size:
650 594 321 318 92 56
BbsI GAAGACnn'nnnn_
Cuts at: 0 190 463 688 886 2031
Size: 190 273 225 198 1145
Fragments arranged by size:
1145 273 225 198 190
BglI GCCn_nnn'nGGC
Cuts at: 0 2027 2031
Size: 2027 4
Bme1580I G_kGCm'C
Cuts at: 0 386 1060 2031
Size: 386 674 971
Fragments arranged by size:
971 674 386
BmrI ACTGGGnnnn_n'
Cuts at: 0 405 1752 2031
Size: 405 1347 279
Fragments arranged by size:
1347 405 279
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 138 2031
Size: 138 1893
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 279 1090 1570 2031
Size: 279 811 480 461
Fragments arranged by size:
811 480 461 279
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 866 1252 2031
Size: 866 386 779
Fragments arranged by size:
866 779 386
BsaI GGTCTCn'nnnn_
Cuts at: 0 254 1454 2031
Size: 254 1200 577
Fragments arranged by size:
1200 577 254
BsaAI yAC'GTr
Cuts at: 0 1279 2031
Size: 1279 752
BsaHI Gr'CG_yC
Cuts at: 0 200 1049 2031
Size: 200 849 982
Fragments arranged by size:
982 849 200
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 277 307 2031
Size: 277 30 1724
Fragments arranged by size:
1724 277 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 340 508 511 514 517 646 736 873
Size: 340 168 3 3 3 129 90 137
Cuts at: 873 949 1147 1162 1663 1894 2031
Size: 76 198 15 501 231 137
Fragments arranged by size:
501 340 231 198 168 137 137 129
90 76 15 3 3 3
BseYI C'CCAG_C
Cuts at: 0 264 1168 2031
Size: 264 904 863
Fragments arranged by size:
904 863 264
BsiHKAI G_wGCw'C
Cuts at: 0 1894 2031
Size: 1894 137
BsmI GAATG_Cn'
Cuts at: 0 669 2031
Size: 669 1362
Bsp1286I G_dGCh'C
Cuts at: 0 318 386 410 1060 1116 1437 1894 2031
Size: 318 68 24 650 56 321 457 137
Fragments arranged by size:
650 457 321 318 137 68 56 24
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1472 2031
Size: 1472 559
BsrBI CCG'CTC
Cuts at: 0 400 2031
Size: 400 1631
BsrDI GCAATG_nn'
Cuts at: 0 1988 2031
Size: 1988 43
BsrFI r'CCGG_y
Cuts at: 0 1884 2031
Size: 1884 147
BssSI C'ACGA_G
Cuts at: 0 286 834 2031
Size: 286 548 1197
Fragments arranged by size:
1197 548 286
BstXI CCAn_nnnn'nTGG
Cuts at: 0 66 715 1445 2031
Size: 66 649 730 586
Fragments arranged by size:
730 649 586 66
Bsu36I CC'TnA_GG
Cuts at: 0 1061 2031
Size: 1061 970
BtgI C'CryG_G
Cuts at: 0 35 275 852 2031
Size: 35 240 577 1179
Fragments arranged by size:
1179 577 240 35
BtsI GCAGTG_nn'
Cuts at: 0 552 2031
Size: 552 1479
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1369 1404 2031
Size: 1369 35 627
Fragments arranged by size:
1369 627 35
DraIII CAC_nnn'GTG
Cuts at: 0 1384 2031
Size: 1384 647
DrdI GACnn_nn'nnGTC
Cuts at: 0 439 1936 2031
Size: 439 1497 95
Fragments arranged by size:
1497 439 95
EaeI y'GGCC_r
Cuts at: 0 641 953 2031
Size: 641 312 1078
Fragments arranged by size:
1078 641 312
EarI CTCTTCn'nnn_
Cuts at: 0 469 545 560 694 1214 1301 1540 1588
Size: 469 76 15 134 520 87 239 48
Cuts at: 1588 1608 1750 2031
Size: 20 142 281
Fragments arranged by size:
520 469 281 239 142 134 87 76
48 20 15
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 279 467 599 986 1090 1570 2031
Size: 202 77 188 132 387 104 480 461
Fragments arranged by size:
480 461 387 202 188 132 104 77
EcoNI CCTnn'n_nnAGG
Cuts at: 0 297 1945 2031
Size: 297 1648 86
Fragments arranged by size:
1648 297 86
EcoO109I rG'GnC_Cy
Cuts at: 0 117 875 1056 1057 1065 1227 1773 2031
Size: 117 758 181 1 8 162 546 258
Fragments arranged by size:
758 546 258 181 162 117 8 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1701 2031
Size: 1701 330
HaeII r_GCGC'y
Cuts at: 0 149 1083 2031
Size: 149 934 948
Fragments arranged by size:
948 934 149
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 408 440 796 828 862 894 2031
Size: 408 32 356 32 34 32 1137
Fragments arranged by size:
1137 408 356 34 32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 455 2031
Size: 455 1576
MscI TGG'CCA
Cuts at: 0 643 955 2031
Size: 643 312 1076
Fragments arranged by size:
1076 643 312
MslI CAynn'nnrTG
Cuts at: 0 64 1199 1337 2031
Size: 64 1135 138 694
Fragments arranged by size:
1135 694 138 64
MspA1I CmG'CkG
Cuts at: 0 268 1534 1852 2031
Size: 268 1266 318 179
Fragments arranged by size:
1266 318 268 179
NspI r_CATG'y
Cuts at: 0 661 2031
Size: 661 1370
PasI CC'CwG_GG
Cuts at: 0 319 1438 2031
Size: 319 1119 593
Fragments arranged by size:
1119 593 319
PmlI CAC'GTG
Cuts at: 0 1279 2031
Size: 1279 752
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1300 1332 2031
Size: 1300 32 699
Fragments arranged by size:
1300 699 32
PpuMI rG'GwC_Cy
Cuts at: 0 117 875 1065 1773 2031
Size: 117 758 190 708 258
Fragments arranged by size:
758 708 258 190 117
PspOMI G'GGCC_C
Cuts at: 0 1056 2031
Size: 1056 975
PstI C_TGCA'G
Cuts at: 0 1469 2031
Size: 1469 562
PvuII CAG'CTG
Cuts at: 0 268 1534 1852 2031
Size: 268 1266 318 179
Fragments arranged by size:
1266 318 268 179
SanDI GG'GwC_CC
Cuts at: 0 1773 2031
Size: 1773 258
SapI GCTCTTCn'nnn_
Cuts at: 0 469 560 1588 2031
Size: 469 91 1028 443
Fragments arranged by size:
1028 469 443 91
SbfI CC_TGCA'GG
Cuts at: 0 1469 2031
Size: 1469 562
SfcI C'TryA_G
Cuts at: 0 993 1083 1465 1836 1996 2031
Size: 993 90 382 371 160 35
Fragments arranged by size:
993 382 371 160 90 35
SmlI C'TyrA_G
Cuts at: 0 881 1231 1639 2031
Size: 881 350 408 392
Fragments arranged by size:
881 408 392 350
StyI C'CwwG_G
Cuts at: 0 336 2031
Size: 336 1695
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 168 1312 1909 2031
Size: 168 1144 597 122
Fragments arranged by size:
1144 597 168 122
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 277 309 1057 1089 2031
Size: 277 32 748 32 942
Fragments arranged by size:
942 748 277 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 816 1385 2031
Size: 816 569 646
Fragments arranged by size:
816 646 569
XhoI C'TCGA_G
Cuts at: 0 1639 2031
Size: 1639 392
XmnI GAAnn'nnTTC
Cuts at: 0 1315 1681 1684 2031
Size: 1315 366 3 347
Fragments arranged by size:
1315 366 347 3
ZraI GAC'GTC
Cuts at: 0 201 2031
Size: 201 1830
Enzymes that do cut:
AatII AcuI AfeI AleI ApaI ApoI AvaI BanII
BbsI BglI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaHI BsaXI BseRI BseYI BsiHKAI BsmI Bsp1286I
BspMI BsrBI BsrDI BsrFI BssSI BstXI Bsu36I BtgI
BtsI CspCI DraIII DrdI EaeI EarI Eco57MI EcoNI
EcoO109I FalI HaeII Hin4I MmeI MscI MslI MspA1I
NspI PasI PmlI PpiI PpuMI PspOMI PstI PvuII
SanDI SapI SbfI SfcI SmlI StyI TaqII TstI
XcmI XhoI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AflII AflIII AgeI AhdI
AlfI AloI AlwNI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BanI BbeI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI BmgBI BmtI Bpu10I BsaBI BsaWI
BsgI BsiEI BsiWI BsmBI BspEI BspHI BsrGI BssHII
BstAPI BstBI BstEII BstYI BstZ17I BtgZI ClaI DraI
EagI EciI EcoICRI EcoRI EcoRV FseI FspI FspAI
HincII HindIII HpaI KasI KpnI MfeI MluI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PciI PflMI PfoI PmeI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SacII SalI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI TatI Tth111I XbaI XmaI