(Circular) (Six-base) MAPSORT of: pFN21AB4991.seq Check: 309 from: 1 to: 5638 pFN21AB4991 5638 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3448 278 Size: 53 83 186 936 1912 2468 Fragments arranged by size: 2468 1912 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2351 2981 1937 Size: 414 630 4594 Fragments arranged by size: 4594 630 414 Acc65I G'GTAC_C Cuts at: 2959 2959 Size: 5638 AclI AA'CG_TT Cuts at: 1563 3765 4138 1563 Size: 2202 373 3063 Fragments arranged by size: 3063 2202 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2357 2541 3701 5085 5353 1632 Size: 725 184 1160 1384 268 1917 Fragments arranged by size: 1917 1384 1160 725 268 184 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5441 AflIII A'CryG_T Cuts at: 1203 4537 5514 1203 Size: 3334 977 1327 Fragments arranged by size: 3334 1327 977 AgeI A'CCGG_T Cuts at: 4469 4469 Size: 5638 AhdI GACnn_n'nnGTC Cuts at: 4367 4367 Size: 5638 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5638 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2120 1184 Size: 936 4702 AlwNI CAG_nnn'CTG Cuts at: 3381 4953 3381 Size: 1572 4066 ApaLI G'TGCA_C Cuts at: 3695 4851 3695 Size: 1156 4482 ApoI r'AATT_y Cuts at: 1493 2315 2947 3173 3325 5434 1493 Size: 822 632 226 152 2109 1697 Fragments arranged by size: 2109 1697 822 632 226 152 AseI AT'TA_AT Cuts at: 160 1032 4192 160 Size: 872 3160 1606 Fragments arranged by size: 3160 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5638 AvaI C'yCGr_G Cuts at: 1870 1943 2080 2963 1870 Size: 73 137 883 4545 Fragments arranged by size: 4545 883 137 73 BamHI G'GATC_C Cuts at: 2968 3335 2968 Size: 367 5271 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2460 2959 4414 618 Size: 325 217 627 673 499 1455 1842 Fragments arranged by size: 1842 1455 673 627 499 325 217 BanII G_rGCy'C Cuts at: 1391 2674 2797 2957 1391 Size: 1283 123 160 4072 Fragments arranged by size: 4072 1283 160 123 BbeI G_GCGC'C Cuts at: 2464 2464 Size: 5638 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4667 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3829 3863 1903 Size: 34 50 34 1808 34 3678 Fragments arranged by size: 3678 1808 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3530 4740 3530 Size: 1210 4428 BclI T'GATC_A Cuts at: 1548 2706 1548 Size: 1158 4480 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2801 4249 136 Size: 107 122 71 631 605 1129 1448 1525 Fragments arranged by size: 1525 1448 1129 631 605 122 107 71 BglII A'GATC_T Cuts at: 5633 5633 Size: 5638 BlpI GC'TnA_GC Cuts at: 3055 3055 Size: 5638 Bme1580I G_kGCm'C Cuts at: 1790 3699 4855 1790 Size: 1909 1156 2573 Fragments arranged by size: 2573 1909 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5638 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2757 3369 4327 Size: 416 53 83 399 24 1340 612 958 Cuts at: 4327 442 Size: 1753 Fragments arranged by size: 1753 1340 958 612 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5638 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4298 1239 Size: 3059 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2500 2772 3763 4628 4926 2500 Size: 272 991 865 298 3212 Fragments arranged by size: 3212 991 865 298 272 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4301 882 Size: 617 2802 2219 Fragments arranged by size: 2802 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2371 3429 493 Size: 711 1167 1058 2702 Fragments arranged by size: 2702 1167 1058 711 BsaBI GATnn'nnATC Cuts at: 5632 5632 Size: 5638 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2461 3445 3827 4462 Size: 53 83 186 936 928 984 382 635 Cuts at: 4462 275 Size: 1451 Fragments arranged by size: 1451 984 936 928 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2322 2877 4071 4469 4743 4890 5301 2322 Size: 555 1194 398 274 147 411 2659 Fragments arranged by size: 2659 1194 555 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2784 2814 1178 Size: 30 165 30 1381 30 4002 Fragments arranged by size: 4002 1381 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2098 2101 2682 1186 Size: 912 3 581 4142 Fragments arranged by size: 4142 912 581 3 BseYI C'CCAG_C Cuts at: 4841 5337 4841 Size: 496 5142 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5065 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2034 2351 3088 3849 4877 1540 Size: 275 186 33 317 737 761 1028 2301 Fragments arranged by size: 2301 1028 761 737 317 275 186 33 BsiHKAI G_wGCw'C Cuts at: 2268 3699 3784 4855 2268 Size: 1431 85 1071 3051 Fragments arranged by size: 3051 1431 1071 85 BsmI GAATG_Cn' Cuts at: 3149 3242 3149 Size: 93 5545 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2268 2674 2797 2957 3699 3784 4855 Size: 399 478 406 123 160 742 85 1071 Cuts at: 4855 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 478 406 399 160 123 85 BspEI T'CCGG_A Cuts at: 2877 5301 2877 Size: 2424 3214 BspHI T'CATG_A Cuts at: 2709 3527 5257 2709 Size: 818 1730 3090 Fragments arranged by size: 3090 1730 818 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2652 2993 844 Size: 1808 341 3489 Fragments arranged by size: 3489 1808 341 BsrBI CCG'CTC Cuts at: 1394 1823 2091 3525 1394 Size: 429 268 1434 3507 Fragments arranged by size: 3507 1434 429 268 BsrDI GCAATG_nn' Cuts at: 65 1248 2215 2286 2439 4133 4307 65 Size: 1183 967 71 153 1694 174 1396 Fragments arranged by size: 1694 1396 1183 967 174 153 71 BsrFI r'CCGG_y Cuts at: 1700 1810 4282 4469 1700 Size: 110 2472 187 2869 Fragments arranged by size: 2869 2472 187 110 BsrGI T'GTAC_A Cuts at: 96 2616 96 Size: 2520 3118 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5638 BssSI C'ACGA_G Cuts at: 3698 4710 3698 Size: 1012 4626 BstAPI GCAn_nnn'nTGC Cuts at: 794 2783 794 Size: 1989 3649 BstBI TT'CG_AA Cuts at: 4442 4442 Size: 5638 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5638 BstYI r'GATC_y Cuts at: 1972 2325 2968 3335 3720 3737 5189 5275 5287 Size: 353 643 367 385 17 1452 86 12 Cuts at: 5287 5633 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 643 385 367 353 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2274 513 Size: 552 1209 3877 Fragments arranged by size: 3877 1209 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4899 Fragments arranged by size: 4899 683 56 BtsI GCAGTG_nn' Cuts at: 3154 3948 3968 3154 Size: 794 20 4824 Fragments arranged by size: 4824 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5638 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5603 DraI TTT'AAA Cuts at: 2942 2942 Size: 5638 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2564 4645 809 Size: 507 1248 2081 1802 Fragments arranged by size: 2081 1802 1248 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2596 3085 3974 Size: 54 1425 50 275 18 766 489 889 Cuts at: 3974 5603 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 766 489 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3085 1537 Size: 275 1273 4090 Fragments arranged by size: 4090 1273 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2132 2141 2840 3568 1353 Size: 779 9 699 728 3423 Fragments arranged by size: 3423 779 728 699 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4225 4599 4745 387 Size: 1064 138 2636 374 146 1280 Fragments arranged by size: 2636 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2357 2541 3701 4298 5085 5353 1239 Size: 393 725 184 1160 597 787 268 1524 Fragments arranged by size: 1524 1160 787 725 597 393 268 184 EcoRI G'AATT_C Cuts at: 2315 2947 3325 2315 Size: 632 378 4628 Fragments arranged by size: 4628 632 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2605 2605 Size: 5638 FspI TGC'GCA Cuts at: 4144 4144 Size: 5638 HaeII r_GCGC'y Cuts at: 1678 2069 2464 4785 1678 Size: 391 395 2321 2531 Fragments arranged by size: 2531 2321 395 391 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4279 4311 4353 4385 1373 Size: 32 135 32 2707 32 42 32 2626 Fragments arranged by size: 2707 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2352 2982 3228 669 Size: 988 281 414 630 246 3079 Fragments arranged by size: 3079 988 630 414 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5638 HpaI GTT'AAC Cuts at: 3228 3228 Size: 5638 KasI G'GCGC_C Cuts at: 2460 2460 Size: 5638 KpnI G_GTAC'C Cuts at: 2963 2963 Size: 5638 MfeI C'AATT_G Cuts at: 3237 3237 Size: 5638 MluI A'CGCG_T Cuts at: 5514 5514 Size: 5638 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4752 4936 1305 Size: 3447 184 2007 Fragments arranged by size: 3447 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2598 10 Size: 54 1425 343 766 3050 Fragments arranged by size: 3050 1425 766 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2763 3596 3955 4114 518 Size: 648 1597 833 359 159 2042 Fragments arranged by size: 2042 1597 833 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3054 3731 4879 5124 1606 Size: 1448 677 1148 245 2120 Fragments arranged by size: 2120 1448 1148 677 245 NarI GG'CG_CC Cuts at: 2461 2461 Size: 5638 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5086 NdeI CA'TA_TG Cuts at: 387 387 Size: 5638 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5638 NotI GC'GGCC_GC Cuts at: 3085 3085 Size: 5638 NruI TCG'CGA Cuts at: 2300 2300 Size: 5638 NspI r_CATG'y Cuts at: 1132 1229 2996 4541 1132 Size: 97 1767 1545 2229 Fragments arranged by size: 2229 1767 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5638 PciI A'CATG_T Cuts at: 4537 4537 Size: 5638 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5638 PfoI T'CCnGG_A Cuts at: 2037 2568 2037 Size: 531 5107 PmeI GTTT'AAAC Cuts at: 2942 2942 Size: 5638 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3707 3739 5220 5252 3707 Size: 32 1481 32 4093 Fragments arranged by size: 4093 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5638 PsiI TTA'TAA Cuts at: 3208 3208 Size: 5638 PstI C_TGCA'G Cuts at: 830 2649 2697 2990 830 Size: 1819 48 293 3478 Fragments arranged by size: 3478 1819 293 48 PvuI CG_AT'CG Cuts at: 2001 2034 2001 Size: 33 5605 SalI G'TCGA_C Cuts at: 1936 2350 2980 1936 Size: 414 630 4594 Fragments arranged by size: 4594 630 414 SbfI CC_TGCA'GG Cuts at: 2649 2990 2649 Size: 341 5297 ScaI AGT'ACT Cuts at: 3886 5598 3886 Size: 1712 3926 SfcI C'TryA_G Cuts at: 826 1045 2539 2645 2693 2986 4121 4802 4993 Size: 219 1494 106 48 293 1135 681 191 Cuts at: 4993 826 Size: 1471 Fragments arranged by size: 1494 1471 1135 681 293 219 191 106 48 SfoI GGC'GCC Cuts at: 2462 2462 Size: 5638 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5638 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5638 SmaI CCC'GGG Cuts at: 1872 2965 1872 Size: 1093 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2479 2787 3742 4643 4905 820 Size: 197 926 536 308 955 901 262 1553 Fragments arranged by size: 1553 955 926 901 536 308 262 197 SnaBI TAC'GTA Cuts at: 493 3429 493 Size: 2936 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5638 SphI G_CATG'C Cuts at: 1132 2996 1132 Size: 1864 3774 SspI AAT'ATT Cuts at: 5 52 3562 5 Size: 47 3510 2081 Fragments arranged by size: 3510 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5638 StyI C'CwwG_G Cuts at: 513 1065 3077 513 Size: 552 2012 3074 Fragments arranged by size: 3074 2012 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3665 3682 3835 4020 4465 325 Size: 1255 2085 17 153 185 445 1498 Fragments arranged by size: 2085 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2616 3884 5596 Size: 274 80 33 51 1443 639 1268 1712 Cuts at: 5596 96 Size: 138 Fragments arranged by size: 1712 1443 1268 639 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2784 2816 5598 5630 2784 Size: 32 2782 32 2792 Fragments arranged by size: 2792 2782 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5638 XbaI T'CTAG_A Cuts at: 2974 2974 Size: 5638 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2770 2770 Size: 5638 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5638 XmaI C'CCGG_G Cuts at: 1870 2963 1870 Size: 1093 4545 XmnI GAAnn'nnTTC Cuts at: 2585 3767 2585 Size: 1182 4456 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3446 276 Size: 53 83 186 936 1912 2468 Fragments arranged by size: 2468 1912 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI NaeI NgoMIV NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI