(Circular) (Six-base) MAPSORT of: pFN21AB4920.seq Check: 4449 from: 1 to: 6988 pFN21AB4920 6988 bp With 182 enzymes: SgfI * February 27, 2009 14:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4798 278 Size: 53 83 186 936 3262 2468 Fragments arranged by size: 3262 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2149 2535 4331 1937 Size: 212 386 1796 4594 Fragments arranged by size: 4594 1796 386 212 Acc65I G'GTAC_C Cuts at: 4309 4309 Size: 6988 AclI AA'CG_TT Cuts at: 1563 5115 5488 1563 Size: 3552 373 3063 Fragments arranged by size: 3552 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3286 3370 3454 3560 3790 5051 6435 6703 Size: 1654 84 84 106 230 1261 1384 268 Cuts at: 6703 1632 Size: 1917 Fragments arranged by size: 1917 1654 1384 1261 268 230 106 84 84 AflII C'TTAA_G Cuts at: 820 1017 2228 820 Size: 197 1211 5580 Fragments arranged by size: 5580 1211 197 AflIII A'CryG_T Cuts at: 1203 4258 5887 6864 1203 Size: 3055 1629 977 1327 Fragments arranged by size: 3055 1629 1327 977 AgeI A'CCGG_T Cuts at: 5819 5819 Size: 6988 AhdI GACnn_n'nnGTC Cuts at: 3555 5717 3555 Size: 2162 4826 AleI CACnn'nnGTG Cuts at: 1166 3288 1166 Size: 2122 4866 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6988 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2017 2049 2017 Size: 32 6956 AlwNI CAG_nnn'CTG Cuts at: 4731 6303 4731 Size: 1572 5416 ApaLI G'TGCA_C Cuts at: 3312 3396 3902 5045 6201 3312 Size: 84 506 1143 1156 4099 Fragments arranged by size: 4099 1156 1143 506 84 ApoI r'AATT_y Cuts at: 1493 2155 2164 2265 2697 2862 3131 3336 3672 Size: 662 9 101 432 165 269 205 336 Cuts at: 3672 4176 4297 4523 4675 6784 1493 Size: 504 121 226 152 2109 1697 Fragments arranged by size: 2109 1697 662 504 432 336 269 226 205 165 152 121 101 9 AseI AT'TA_AT Cuts at: 160 1032 3492 5542 160 Size: 872 2460 2050 1606 Fragments arranged by size: 2460 2050 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6988 AvaI C'yCGr_G Cuts at: 1870 1943 4313 1870 Size: 73 2370 4545 Fragments arranged by size: 4545 2370 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3151 3184 3151 Size: 33 6955 BamHI G'GATC_C Cuts at: 4318 4685 4318 Size: 367 6621 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4271 4309 5764 618 Size: 325 217 627 2484 38 1455 1842 Fragments arranged by size: 2484 1842 1455 627 325 217 38 BanII G_rGCy'C Cuts at: 1391 2223 4307 1391 Size: 832 2084 4072 Fragments arranged by size: 4072 2084 832 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2454 2811 3554 928 Size: 971 555 357 743 4362 Fragments arranged by size: 4362 971 743 555 357 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2205 2239 5179 5213 1903 Size: 34 268 34 2940 34 3678 Fragments arranged by size: 3678 2940 268 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2352 4880 6090 2352 Size: 2528 1210 3250 Fragments arranged by size: 3250 2528 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6988 BfrBI ATG'CAT Cuts at: 4202 4202 Size: 6988 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5599 136 Size: 107 122 71 631 605 3927 1525 Fragments arranged by size: 3927 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6983 6983 Size: 6988 BlpI GC'TnA_GC Cuts at: 4405 4405 Size: 6988 Bme1580I G_kGCm'C Cuts at: 1790 3316 3400 3906 5049 6205 1790 Size: 1526 84 506 1143 1156 2573 Fragments arranged by size: 2573 1526 1156 1143 506 84 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6988 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2332 2563 3606 Size: 416 53 83 399 24 915 231 1043 Cuts at: 3606 4719 5677 442 Size: 1113 958 1753 Fragments arranged by size: 1753 1113 1043 958 915 416 399 231 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6988 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2269 2269 Size: 6988 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2306 2733 3114 3282 3366 3450 3534 3702 Size: 1067 427 381 168 84 84 84 168 Cuts at: 3702 3786 3870 4038 4122 5648 1239 Size: 84 84 168 84 1526 2579 Fragments arranged by size: 2579 1526 1067 427 381 168 168 168 84 84 84 84 84 84 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1999 5113 5978 6276 1999 Size: 3114 865 298 2711 Fragments arranged by size: 3114 2711 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2135 2233 4102 5651 882 Size: 617 636 98 1869 1549 2219 Fragments arranged by size: 2219 1869 1549 636 617 98 BsaAI yAC'GTr Cuts at: 493 1204 4779 493 Size: 711 3575 2702 Fragments arranged by size: 3575 2702 711 BsaBI GATnn'nnATC Cuts at: 6982 6982 Size: 6988 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4795 5177 5812 275 Size: 53 83 186 936 3262 382 635 1451 Fragments arranged by size: 3262 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5421 5819 6093 6240 6651 5421 Size: 398 274 147 411 5758 Fragments arranged by size: 5758 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2747 2777 1178 Size: 30 165 30 1344 30 5389 Fragments arranged by size: 5389 1344 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2400 1186 Size: 1214 5774 BseYI C'CCAG_C Cuts at: 6191 6687 6191 Size: 496 6492 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2594 1163 Size: 573 858 5557 Fragments arranged by size: 5557 858 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4438 5199 6227 1540 Size: 275 186 2437 761 1028 2301 Fragments arranged by size: 2437 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2223 3316 3400 3906 5049 5134 6205 2223 Size: 1093 84 506 1143 85 1071 3006 Fragments arranged by size: 3006 1143 1093 1071 506 85 84 BsmI GAATG_Cn' Cuts at: 2974 3258 3284 3887 4499 4592 2974 Size: 284 26 603 612 93 5370 Fragments arranged by size: 5370 612 603 284 93 26 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2223 3316 3400 3906 4307 5049 5134 Size: 399 433 1093 84 506 401 742 85 Cuts at: 5134 6205 1391 Size: 1071 2174 Fragments arranged by size: 2174 1093 1071 742 506 433 401 399 85 84 BspEI T'CCGG_A Cuts at: 6651 6651 Size: 6988 BspHI T'CATG_A Cuts at: 4877 6607 4877 Size: 1730 5258 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4343 844 Size: 3499 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4875 1394 Size: 429 3052 3507 Fragments arranged by size: 3507 3052 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3541 3625 5483 5657 65 Size: 1183 2293 84 1858 174 1396 Fragments arranged by size: 2293 1858 1396 1183 174 84 BsrFI r'CCGG_y Cuts at: 1700 1810 5632 5819 1700 Size: 110 3822 187 2869 Fragments arranged by size: 3822 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2643 2989 3073 96 Size: 2547 346 84 4011 Fragments arranged by size: 4011 2547 346 84 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6988 BssSI C'ACGA_G Cuts at: 5048 6060 5048 Size: 1012 5976 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6988 BstBI TT'CG_AA Cuts at: 5792 5792 Size: 6988 BstEII G'GTnAC_C Cuts at: 1184 2497 1184 Size: 1313 5675 BstXI CCAn_nnnn'nTGG Cuts at: 3290 3290 Size: 6988 BstYI r'GATC_y Cuts at: 1972 4318 4685 5070 5087 6539 6625 6637 6983 Size: 2346 367 385 17 1452 86 12 346 Cuts at: 6983 1972 Size: 1977 Fragments arranged by size: 2346 1977 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2678 513 Size: 552 1613 4823 Fragments arranged by size: 4823 1613 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6249 Fragments arranged by size: 6249 683 56 BtsI GCAGTG_nn' Cuts at: 2370 3877 4504 5298 5318 2370 Size: 1507 627 794 20 4040 Fragments arranged by size: 4040 1507 794 627 20 ClaI AT'CG_AT Cuts at: 1554 2510 1554 Size: 956 6032 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3523 3558 3943 3978 529 Size: 35 2959 35 385 35 3539 Fragments arranged by size: 3539 2959 385 35 35 35 DraI TTT'AAA Cuts at: 3949 4292 3949 Size: 343 6645 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5995 809 Size: 507 4679 1802 Fragments arranged by size: 4679 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4435 5324 6953 Size: 54 1425 50 275 18 2605 889 1629 Cuts at: 6953 8 Size: 43 Fragments arranged by size: 2605 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4435 1537 Size: 275 2623 4090 Fragments arranged by size: 4090 2623 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2394 4918 1353 Size: 1041 2524 3423 Fragments arranged by size: 3423 2524 1041 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5575 5949 6095 387 Size: 1064 138 3986 374 146 1280 Fragments arranged by size: 3986 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2221 2221 Size: 6988 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2306 2733 3114 3282 3286 3366 3370 Size: 393 674 427 381 168 4 80 4 Cuts at: 3370 3450 3454 3534 3560 3702 3786 3790 3870 Size: 80 4 80 26 142 84 4 80 Cuts at: 3870 4038 4122 5051 5648 6435 6703 1239 Size: 168 84 929 597 787 268 1524 Fragments arranged by size: 1524 929 787 674 597 427 393 381 268 168 168 142 84 84 80 80 80 80 26 4 4 4 4 EcoO109I rG'GnC_Cy Cuts at: 2708 2708 Size: 6988 EcoRI G'AATT_C Cuts at: 3336 3672 4176 4297 4675 3336 Size: 336 504 121 378 5649 Fragments arranged by size: 5649 504 378 336 121 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2951 2951 Size: 6988 FspI TGC'GCA Cuts at: 5494 5494 Size: 6988 HaeII r_GCGC'y Cuts at: 1678 6135 1678 Size: 4457 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2747 2779 3481 3513 3541 Size: 32 135 32 1175 32 702 32 28 Cuts at: 3541 3573 3805 3837 5629 5661 5703 5735 1373 Size: 32 232 32 1792 32 42 32 2626 Fragments arranged by size: 2626 1792 1175 702 232 135 42 32 32 32 32 32 32 32 32 28 HincII GTy'rAC Cuts at: 669 1657 1938 2150 2210 4332 4578 669 Size: 988 281 212 60 2122 246 3079 Fragments arranged by size: 3079 2122 988 281 246 212 60 HindIII A'AGCT_T Cuts at: 748 4053 748 Size: 3305 3683 HpaI GTT'AAC Cuts at: 4578 4578 Size: 6988 KpnI G_GTAC'C Cuts at: 4313 4313 Size: 6988 MfeI C'AATT_G Cuts at: 4587 4587 Size: 6988 MluI A'CGCG_T Cuts at: 6864 6864 Size: 6988 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3608 6102 6286 1305 Size: 2303 2494 184 2007 Fragments arranged by size: 2494 2303 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5166 Fragments arranged by size: 5166 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2871 3277 3288 3445 3613 3865 4257 Size: 648 1705 406 11 157 168 252 392 Cuts at: 4257 4269 4284 4946 5305 5464 518 Size: 12 15 662 359 159 2042 Fragments arranged by size: 2042 1705 662 648 406 392 359 252 168 159 157 15 12 11 MspA1I CmG'CkG Cuts at: 1606 4404 5081 6229 6474 1606 Size: 2798 677 1148 245 2120 Fragments arranged by size: 2798 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2678 513 Size: 552 1613 4823 Fragments arranged by size: 4823 1613 552 NdeI CA'TA_TG Cuts at: 387 2812 3064 3274 387 Size: 2425 252 210 4101 Fragments arranged by size: 4101 2425 252 210 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6988 NotI GC'GGCC_GC Cuts at: 4435 4435 Size: 6988 NsiI A_TGCA'T Cuts at: 4204 4204 Size: 6988 NspI r_CATG'y Cuts at: 1132 1229 2468 2745 4158 4206 4346 5891 1132 Size: 97 1239 277 1413 48 140 1545 2229 Fragments arranged by size: 2229 1545 1413 1239 277 140 97 48 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6988 PciI A'CATG_T Cuts at: 5887 5887 Size: 6988 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6988 PfoI T'CCnGG_A Cuts at: 3091 3091 Size: 6988 PmeI GTTT'AAAC Cuts at: 4292 4292 Size: 6988 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5057 5089 6570 6602 5057 Size: 32 1481 32 5443 Fragments arranged by size: 5443 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3387 1613 Size: 1774 5214 PsiI TTA'TAA Cuts at: 3696 4558 3696 Size: 862 6126 PstI C_TGCA'G Cuts at: 830 4340 830 Size: 3510 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6988 SacI G_AGCT'C Cuts at: 2223 2223 Size: 6988 SalI G'TCGA_C Cuts at: 1936 2148 4330 1936 Size: 212 2182 4594 Fragments arranged by size: 4594 2182 212 SbfI CC_TGCA'GG Cuts at: 4340 4340 Size: 6988 ScaI AGT'ACT Cuts at: 3649 5236 6948 3649 Size: 1587 1712 3689 Fragments arranged by size: 3689 1712 1587 SfcI C'TryA_G Cuts at: 826 1045 2532 2922 4336 5471 6152 6343 826 Size: 219 1487 390 1414 1135 681 191 1471 Fragments arranged by size: 1487 1471 1414 1135 681 390 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6988 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6988 SmaI CCC'GGG Cuts at: 1872 4315 1872 Size: 2443 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2014 2228 5092 5993 6255 820 Size: 197 926 71 214 2864 901 262 1553 Fragments arranged by size: 2864 1553 926 901 262 214 197 71 SnaBI TAC'GTA Cuts at: 493 4779 493 Size: 4286 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6988 SphI G_CATG'C Cuts at: 1132 4346 1132 Size: 3214 3774 SspI AAT'ATT Cuts at: 5 52 4912 5 Size: 47 4860 2081 Fragments arranged by size: 4860 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6988 StyI C'CwwG_G Cuts at: 513 1065 2678 4427 513 Size: 552 1613 1749 3074 Fragments arranged by size: 3074 1749 1613 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2558 4244 5015 5032 5185 5370 5815 Size: 1255 978 1686 771 17 153 185 445 Cuts at: 5815 325 Size: 1498 Fragments arranged by size: 1686 1498 1255 978 771 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2643 2744 2989 Size: 274 80 33 51 1443 666 101 245 Cuts at: 2989 3073 3563 3647 4235 5234 6946 96 Size: 84 490 84 588 999 1712 138 Fragments arranged by size: 1712 1443 999 666 588 490 274 245 138 101 84 84 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2625 2657 6948 6980 2625 Size: 32 4291 32 2633 Fragments arranged by size: 4291 2633 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6988 XbaI T'CTAG_A Cuts at: 2395 4324 2395 Size: 1929 5059 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6988 XmaI C'CCGG_G Cuts at: 1870 4313 1870 Size: 2443 4545 XmnI GAAnn'nnTTC Cuts at: 3383 3551 3676 3803 3887 4055 5117 3383 Size: 168 125 127 84 168 1062 5254 Fragments arranged by size: 5254 1062 168 168 127 125 84 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4796 276 Size: 53 83 186 936 3262 2468 Fragments arranged by size: 3262 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BbeI BbvCI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI