Restriction map for
pFN21AB4920
(Linear) (Six-base) MAPSORT of: KIBB4920.seq Check: 4558 from: 1 to: 2283
KIBB4920.orf
With 182 enzymes: SgfI *
July 18, 2007 13:37 ..
AccI GT'mk_AC
Cuts at: 0 144 530 2283
Size: 144 386 1753
Fragments arranged by size:
1753 386 144
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1281 1365 1449 1555 1785 2283
Size: 1281 84 84 106 230 498
Fragments arranged by size:
1281 498 230 106 84 84
AflII C'TTAA_G
Cuts at: 0 223 2283
Size: 223 2060
AflIII A'CryG_T
Cuts at: 0 2253 2283
Size: 2253 30
AhdI GACnn_n'nnGTC
Cuts at: 0 1550 2283
Size: 1550 733
AleI CACnn'nnGTG
Cuts at: 0 1283 2283
Size: 1283 1000
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 12 44 2283
Size: 12 32 2239
Fragments arranged by size:
2239 32 12
ApaLI G'TGCA_C
Cuts at: 0 1307 1391 1897 2283
Size: 1307 84 506 386
Fragments arranged by size:
1307 506 386 84
ApoI r'AATT_y
Cuts at: 0 150 159 260 692 857 1126 1331 1667
Size: 150 9 101 432 165 269 205 336
Cuts at: 1667 2171 2283
Size: 504 112
Fragments arranged by size:
504 432 336 269 205 165 150 112
101 9
AseI AT'TA_AT
Cuts at: 0 1487 2283
Size: 1487 796
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1146 1179 2283
Size: 1146 33 1104
Fragments arranged by size:
1146 1104 33
BanI G'GyrC_C
Cuts at: 0 2266 2283
Size: 2266 17
BanII G_rGCy'C
Cuts at: 0 218 2283
Size: 218 2065
BbsI GAAGACnn'nnnn_
Cuts at: 0 449 806 1549 2283
Size: 449 357 743 734
Fragments arranged by size:
743 734 449 357
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 200 234 2283
Size: 200 34 2049
Fragments arranged by size:
2049 200 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 347 2283
Size: 347 1936
BfrBI ATG'CAT
Cuts at: 0 2197 2283
Size: 2197 86
Bme1580I G_kGCm'C
Cuts at: 0 1311 1395 1901 2283
Size: 1311 84 506 382
Fragments arranged by size:
1311 506 382 84
BmrI ACTGGGnnnn_n'
Cuts at: 0 327 558 1601 2283
Size: 327 231 1043 682
Fragments arranged by size:
1043 682 327 231
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 264 2283
Size: 264 2019
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 301 728 1109 1277 1361 1445 1529 1697
Size: 301 427 381 168 84 84 84 168
Cuts at: 1697 1781 1865 2033 2117 2283
Size: 84 84 168 84 166
Fragments arranged by size:
427 381 301 168 168 168 166 84
84 84 84 84 84
BsaI GGTCTCn'nnnn_
Cuts at: 0 130 228 2097 2283
Size: 130 98 1869 186
Fragments arranged by size:
1869 186 130 98
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 742 772 2283
Size: 742 30 1511
Fragments arranged by size:
1511 742 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 395 2283
Size: 395 1888
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 589 2283
Size: 589 1694
BsiHKAI G_wGCw'C
Cuts at: 0 218 1311 1395 1901 2283
Size: 218 1093 84 506 382
Fragments arranged by size:
1093 506 382 218 84
BsmI GAATG_Cn'
Cuts at: 0 969 1253 1279 1882 2283
Size: 969 284 26 603 401
Fragments arranged by size:
969 603 401 284 26
Bsp1286I G_dGCh'C
Cuts at: 0 218 1311 1395 1901 2283
Size: 218 1093 84 506 382
Fragments arranged by size:
1093 506 382 218 84
BsrDI GCAATG_nn'
Cuts at: 0 1536 1620 2283
Size: 1536 84 663
Fragments arranged by size:
1536 663 84
BsrGI T'GTAC_A
Cuts at: 0 638 984 1068 2283
Size: 638 346 84 1215
Fragments arranged by size:
1215 638 346 84
BstEII G'GTnAC_C
Cuts at: 0 492 2283
Size: 492 1791
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1285 2283
Size: 1285 998
BtgI C'CryG_G
Cuts at: 0 673 2283
Size: 673 1610
BtsI GCAGTG_nn'
Cuts at: 0 365 1872 2283
Size: 365 1507 411
Fragments arranged by size:
1507 411 365
ClaI AT'CG_AT
Cuts at: 0 505 2283
Size: 505 1778
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1518 1553 1938 1973 2283
Size: 1518 35 385 35 310
Fragments arranged by size:
1518 385 310 35 35
DraI TTT'AAA
Cuts at: 0 1944 2283
Size: 1944 339
EarI CTCTTCn'nnn_
Cuts at: 0 389 2283
Size: 389 1894
EcoICRI GAG'CTC
Cuts at: 0 216 2283
Size: 216 2067
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 301 728 1109 1277 1281 1361 1365 1445
Size: 301 427 381 168 4 80 4 80
Cuts at: 1445 1449 1529 1555 1697 1781 1785 1865 2033
Size: 4 80 26 142 84 4 80 168
Cuts at: 2033 2117 2283
Size: 84 166
Fragments arranged by size:
427 381 301 168 168 166 142 84
84 80 80 80 80 26 4 4
4 4
EcoO109I rG'GnC_Cy
Cuts at: 0 703 2283
Size: 703 1580
EcoRI G'AATT_C
Cuts at: 0 1331 1667 2171 2283
Size: 1331 336 504 112
Fragments arranged by size:
1331 504 336 112
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 946 2283
Size: 946 1337
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 742 774 1476 1508 1536 1568 1800 1832
Size: 742 32 702 32 28 32 232 32
Cuts at: 1832 2283
Size: 451
Fragments arranged by size:
742 702 451 232 32 32 32 32
28
HincII GTy'rAC
Cuts at: 0 145 205 2283
Size: 145 60 2078
Fragments arranged by size:
2078 145 60
HindIII A'AGCT_T
Cuts at: 0 2048 2283
Size: 2048 235
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1603 2283
Size: 1603 680
MslI CAynn'nnrTG
Cuts at: 0 866 1272 1283 1440 1608 1860 2252 2264
Size: 866 406 11 157 168 252 392 12
Cuts at: 2264 2283
Size: 19
Fragments arranged by size:
866 406 392 252 168 157 19 12
11
NcoI C'CATG_G
Cuts at: 0 673 2283
Size: 673 1610
NdeI CA'TA_TG
Cuts at: 0 807 1059 1269 2283
Size: 807 252 210 1014
Fragments arranged by size:
1014 807 252 210
NsiI A_TGCA'T
Cuts at: 0 2199 2283
Size: 2199 84
NspI r_CATG'y
Cuts at: 0 463 740 2153 2201 2283
Size: 463 277 1413 48 82
Fragments arranged by size:
1413 463 277 82 48
PfoI T'CCnGG_A
Cuts at: 0 1086 2283
Size: 1086 1197
PshAI GACnn'nnGTC
Cuts at: 0 1382 2283
Size: 1382 901
PsiI TTA'TAA
Cuts at: 0 1691 2283
Size: 1691 592
SacI G_AGCT'C
Cuts at: 0 218 2283
Size: 218 2065
SalI G'TCGA_C
Cuts at: 0 143 2283
Size: 143 2140
ScaI AGT'ACT
Cuts at: 0 1644 2283
Size: 1644 639
SfcI C'TryA_G
Cuts at: 0 527 917 2283
Size: 527 390 1366
Fragments arranged by size:
1366 527 390
SmlI C'TyrA_G
Cuts at: 0 9 223 2283
Size: 9 214 2060
Fragments arranged by size:
2060 214 9
StyI C'CwwG_G
Cuts at: 0 673 2283
Size: 673 1610
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 553 2239 2283
Size: 553 1686 44
Fragments arranged by size:
1686 553 44
TatI w'GTAC_w
Cuts at: 0 638 739 984 1068 1558 1642 2230 2283
Size: 638 101 245 84 490 84 588 53
Fragments arranged by size:
638 588 490 245 101 84 84 53
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 620 652 2283
Size: 620 32 1631
Fragments arranged by size:
1631 620 32
XbaI T'CTAG_A
Cuts at: 0 390 2283
Size: 390 1893
XmnI GAAnn'nnTTC
Cuts at: 0 1378 1546 1671 1798 1882 2050 2283
Size: 1378 168 125 127 84 168 233
Fragments arranged by size:
1378 233 168 168 127 125 84
Enzymes that do cut:
AccI AcuI AflII AflIII AhdI AleI AloI ApaLI
ApoI AseI BaeI BanI BanII BbsI BcgI BciVI
BfrBI Bme1580I BmrI BplI BpmI BsaI BsaXI BseRI
BsgI BsiHKAI BsmI Bsp1286I BsrDI BsrGI BstEII BstXI
BtgI BtsI ClaI CspCI DraI EarI EcoICRI Eco57MI
EcoO109I EcoRI FalI Hin4I HincII HindIII MmeI MslI
NcoI NdeI NsiI NspI PfoI PshAI PsiI SacI
SalI ScaI SfcI SmlI StyI TaqII TatI TstI
XbaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AlfI AlwNI
ApaI AscI AsiSI AvaI AvrII BamHI BbeI BbvCI
BclI BglI BglII BlpI BmgBI BmtI Bpu10I BpuEI
BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiWI BsmBI
BspEI BspHI BspMI BsrBI BsrFI BssHII BssSI BstAPI
BstBI BstYI BstZ17I Bsu36I BtgZI DraIII DrdI EaeI
EagI EciI EcoNI EcoRV FseI FspI FspAI HaeII
HpaI KasI KpnI MfeI MluI MscI MspA1I NaeI
NarI NgoMIV NheI NotI NruI PacI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PspOMI PspXI PsrI
PstI PvuI PvuII RsrII SacII SanDI SapI SbfI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI Tth111I XcmI XhoI
XmaI ZraI