(Circular) (Six-base) MAPSORT of: pFN21AB4902.seq Check: 5159 from: 1 to: 6937 pFN21AB4902 6937 bp With 182 enzymes: SgfI * February 27, 2009 14:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4747 278 Size: 53 83 186 936 3211 2468 Fragments arranged by size: 3211 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3276 4280 1937 Size: 1339 1004 4594 Fragments arranged by size: 4594 1339 1004 Acc65I G'GTAC_C Cuts at: 4258 4258 Size: 6937 AclI AA'CG_TT Cuts at: 1563 5064 5437 1563 Size: 3501 373 3063 Fragments arranged by size: 3501 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2171 2504 2952 3944 5000 6384 6652 1632 Size: 539 333 448 992 1056 1384 268 1917 Fragments arranged by size: 1917 1384 1056 992 539 448 333 268 AflII C'TTAA_G Cuts at: 820 1017 4201 820 Size: 197 3184 3556 Fragments arranged by size: 3556 3184 197 AflIII A'CryG_T Cuts at: 1203 2758 5836 6813 1203 Size: 1555 3078 977 1327 Fragments arranged by size: 3078 1555 1327 977 AgeI A'CCGG_T Cuts at: 5768 5768 Size: 6937 AhdI GACnn_n'nnGTC Cuts at: 5666 5666 Size: 6937 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6937 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6937 AlwNI CAG_nnn'CTG Cuts at: 3067 4680 6252 3067 Size: 1613 1572 3752 Fragments arranged by size: 3752 1613 1572 ApaLI G'TGCA_C Cuts at: 4994 6150 4994 Size: 1156 5781 ApoI r'AATT_y Cuts at: 1493 2363 2572 2681 3307 4187 4246 4472 4624 Size: 870 209 109 626 880 59 226 152 Cuts at: 4624 6733 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 880 870 626 226 209 152 109 59 AseI AT'TA_AT Cuts at: 160 1032 5491 160 Size: 872 4459 1606 Fragments arranged by size: 4459 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6937 AvaI C'yCGr_G Cuts at: 1870 1943 2715 3549 4262 1870 Size: 73 772 834 713 4545 Fragments arranged by size: 4545 834 772 713 73 BamHI G'GATC_C Cuts at: 2470 4165 4267 4634 2470 Size: 1695 102 367 4773 Fragments arranged by size: 4773 1695 367 102 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3901 4258 5713 618 Size: 325 217 627 2114 357 1455 1842 Fragments arranged by size: 2114 1842 1455 627 357 325 217 BanII G_rGCy'C Cuts at: 1391 2850 4256 1391 Size: 1459 1406 4072 Fragments arranged by size: 4072 1459 1406 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2159 3172 3656 928 Size: 971 260 1013 484 4209 Fragments arranged by size: 4209 1013 971 484 260 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2556 2590 5128 5162 1903 Size: 34 619 34 2538 34 3678 Fragments arranged by size: 3678 2538 619 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4829 6039 4829 Size: 1210 5727 BclI T'GATC_A Cuts at: 1548 1548 Size: 6937 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5548 136 Size: 107 122 71 631 605 3876 1525 Fragments arranged by size: 3876 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3133 3702 3765 6932 3133 Size: 569 63 3167 3138 Fragments arranged by size: 3167 3138 569 63 BlpI GC'TnA_GC Cuts at: 4354 4354 Size: 6937 Bme1580I G_kGCm'C Cuts at: 1790 4008 4998 6154 1790 Size: 2218 990 1156 2573 Fragments arranged by size: 2573 2218 1156 990 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6937 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3343 3536 4668 Size: 416 53 83 399 24 1926 193 1132 Cuts at: 4668 5626 442 Size: 958 1753 Fragments arranged by size: 1926 1753 1132 958 416 399 193 83 53 24 BmtI G_CTAG'C Cuts at: 1056 4221 1056 Size: 3165 3772 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2139 2139 Size: 6937 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2712 5597 1239 Size: 1473 2885 2579 Fragments arranged by size: 2885 2579 1473 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5062 5927 6225 5062 Size: 865 298 5774 Fragments arranged by size: 5774 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3293 5600 882 Size: 617 1794 2307 2219 Fragments arranged by size: 2307 2219 1794 617 BsaAI yAC'GTr Cuts at: 493 1204 4003 4728 493 Size: 711 2799 725 2702 Fragments arranged by size: 2799 2702 725 711 BsaBI GATnn'nnATC Cuts at: 4121 6931 4121 Size: 2810 4127 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4744 5126 5761 275 Size: 53 83 186 936 3211 382 635 1451 Fragments arranged by size: 3211 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5370 5768 6042 6189 6600 5370 Size: 398 274 147 411 5707 Fragments arranged by size: 5707 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2938 2968 1178 Size: 30 165 30 1535 30 5147 Fragments arranged by size: 5147 1535 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2951 3450 3627 3897 1186 Size: 1765 499 177 270 4226 Fragments arranged by size: 4226 1765 499 270 177 BseYI C'CCAG_C Cuts at: 2213 3789 6140 6636 2213 Size: 1576 2351 496 2514 Fragments arranged by size: 2514 2351 1576 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2088 3599 1163 Size: 573 352 1511 4501 Fragments arranged by size: 4501 1511 573 352 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4387 5148 6176 1540 Size: 275 186 2386 761 1028 2301 Fragments arranged by size: 2386 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4998 5083 6154 4998 Size: 85 1071 5781 Fragments arranged by size: 5781 1071 85 BsmI GAATG_Cn' Cuts at: 2186 2842 4448 4541 2186 Size: 656 1606 93 4582 Fragments arranged by size: 4582 1606 656 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2850 4008 4256 4998 5083 6154 1391 Size: 399 1060 1158 248 742 85 1071 2174 Fragments arranged by size: 2174 1158 1071 1060 742 399 248 85 BspEI T'CCGG_A Cuts at: 6600 6600 Size: 6937 BspHI T'CATG_A Cuts at: 3043 4826 6556 3043 Size: 1783 1730 3424 Fragments arranged by size: 3424 1783 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4292 844 Size: 3448 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4824 1394 Size: 429 3001 3507 Fragments arranged by size: 3507 3001 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2181 4011 5432 5606 65 Size: 1183 933 1830 1421 174 1396 Fragments arranged by size: 1830 1421 1396 1183 933 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5581 5768 1700 Size: 110 3771 187 2869 Fragments arranged by size: 3771 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6937 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6937 BssSI C'ACGA_G Cuts at: 4997 6009 4997 Size: 1012 5925 BstAPI GCAn_nnn'nTGC Cuts at: 794 3671 794 Size: 2877 4060 BstBI TT'CG_AA Cuts at: 5741 5741 Size: 6937 BstEII G'GTnAC_C Cuts at: 1184 3536 1184 Size: 2352 4585 BstYI r'GATC_y Cuts at: 1972 2470 3133 3702 3765 4165 4267 4634 5019 Size: 498 663 569 63 400 102 367 385 Cuts at: 5019 5036 6488 6574 6586 6932 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 663 569 498 400 385 367 346 102 86 63 17 12 Bsu36I CC'TnA_GG Cuts at: 3909 3909 Size: 6937 BtgI C'CryG_G Cuts at: 513 1065 2004 2466 3294 513 Size: 552 939 462 828 4156 Fragments arranged by size: 4156 939 828 552 462 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6198 Fragments arranged by size: 6198 683 56 BtsI GCAGTG_nn' Cuts at: 3354 4453 5247 5267 3354 Size: 1099 794 20 5024 Fragments arranged by size: 5024 1099 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6937 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6902 DraI TTT'AAA Cuts at: 4241 4241 Size: 6937 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3053 5944 809 Size: 507 1737 2891 1802 Fragments arranged by size: 2891 1802 1737 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2098 3291 4384 Size: 54 1425 50 275 18 268 1193 1093 Cuts at: 4384 5273 6902 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1193 1093 889 275 268 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4384 1537 Size: 275 2572 4090 Fragments arranged by size: 4090 2572 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2928 3156 3885 4867 1353 Size: 1575 228 729 982 3423 Fragments arranged by size: 3423 1575 982 729 228 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5524 5898 6044 387 Size: 1064 138 3935 374 146 1280 Fragments arranged by size: 3935 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2171 2504 2712 2952 3944 5000 5597 Size: 393 539 333 208 240 992 1056 597 Cuts at: 5597 6384 6652 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1056 992 787 597 539 393 333 268 240 208 EcoNI CCTnn'n_nnAGG Cuts at: 2499 2499 Size: 6937 EcoO109I rG'GnC_Cy Cuts at: 2118 3632 2118 Size: 1514 5423 EcoRI G'AATT_C Cuts at: 4246 4624 4246 Size: 378 6559 EcoRV GAT'ATC Cuts at: 3466 3466 Size: 6937 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2824 3784 3974 2824 Size: 960 190 5787 Fragments arranged by size: 5787 960 190 FspI TGC'GCA Cuts at: 5443 5443 Size: 6937 HaeII r_GCGC'y Cuts at: 1678 6084 1678 Size: 4406 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2036 2068 2078 2110 3238 Size: 32 135 32 464 32 10 32 1128 Cuts at: 3238 3270 3694 3726 5578 5610 5652 5684 1373 Size: 32 424 32 1852 32 42 32 2626 Fragments arranged by size: 2626 1852 1128 464 424 135 42 32 32 32 32 32 32 32 32 10 HincII GTy'rAC Cuts at: 669 1657 1938 3058 4281 4527 669 Size: 988 281 1120 1223 246 3079 Fragments arranged by size: 3079 1223 1120 988 281 246 HindIII A'AGCT_T Cuts at: 748 2432 3920 4038 748 Size: 1684 1488 118 3647 Fragments arranged by size: 3647 1684 1488 118 HpaI GTT'AAC Cuts at: 4527 4527 Size: 6937 KpnI G_GTAC'C Cuts at: 4262 4262 Size: 6937 MfeI C'AATT_G Cuts at: 2062 4083 4536 2062 Size: 2021 453 4463 Fragments arranged by size: 4463 2021 453 MluI A'CGCG_T Cuts at: 6813 6813 Size: 6937 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2355 2518 2722 3708 4014 6051 6235 1305 Size: 1050 163 204 986 306 2037 184 2007 Fragments arranged by size: 2037 2007 1050 986 306 204 184 163 MscI TGG'CCA Cuts at: 10 64 1489 1832 2100 10 Size: 54 1425 343 268 4847 Fragments arranged by size: 4847 1425 343 268 54 MslI CAynn'nnrTG Cuts at: 518 1166 3116 3359 4001 4895 5254 5413 518 Size: 648 1950 243 642 894 359 159 2042 Fragments arranged by size: 2042 1950 894 648 642 359 243 159 MspA1I CmG'CkG Cuts at: 1606 4030 4353 5030 6178 6423 1606 Size: 2424 323 677 1148 245 2120 Fragments arranged by size: 2424 2120 1148 677 323 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2466 513 Size: 552 939 462 4984 Fragments arranged by size: 4984 939 552 462 NdeI CA'TA_TG Cuts at: 387 387 Size: 6937 NheI G'CTAG_C Cuts at: 1052 4217 1052 Size: 3165 3772 NotI GC'GGCC_GC Cuts at: 4384 4384 Size: 6937 NspI r_CATG'y Cuts at: 1132 1229 2023 2108 2762 4295 5840 1132 Size: 97 794 85 654 1533 1545 2229 Fragments arranged by size: 2229 1545 1533 794 654 97 85 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6937 PciI A'CATG_T Cuts at: 2758 5836 2758 Size: 3078 3859 PflMI CCAn_nnn'nTGG Cuts at: 1108 2795 3479 1108 Size: 1687 684 4566 Fragments arranged by size: 4566 1687 684 PmeI GTTT'AAAC Cuts at: 4241 4241 Size: 6937 PmlI CAC'GTG Cuts at: 4003 4003 Size: 6937 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5006 5038 6519 6551 5006 Size: 32 1481 32 5392 Fragments arranged by size: 5392 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2118 2118 Size: 6937 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6937 PsiI TTA'TAA Cuts at: 4507 4507 Size: 6937 PspXI vC'TCGA_Gb Cuts at: 3549 3549 Size: 6937 PstI C_TGCA'G Cuts at: 830 2313 4289 830 Size: 1483 1976 3478 Fragments arranged by size: 3478 1976 1483 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6937 PvuII CAG'CTG Cuts at: 4030 4030 Size: 6937 SalI G'TCGA_C Cuts at: 1936 4279 1936 Size: 2343 4594 SapI GCTCTTCn'nnn_ Cuts at: 3156 3885 3156 Size: 729 6208 SbfI CC_TGCA'GG Cuts at: 4289 4289 Size: 6937 ScaI AGT'ACT Cuts at: 3385 5185 6897 3385 Size: 1800 1712 3425 Fragments arranged by size: 3425 1800 1712 SfcI C'TryA_G Cuts at: 826 1045 2040 2309 3215 4157 4285 5420 6101 Size: 219 995 269 906 942 128 1135 681 Cuts at: 6101 6292 826 Size: 191 1471 Fragments arranged by size: 1471 1135 995 942 906 681 269 219 191 128 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6937 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6937 SmaI CCC'GGG Cuts at: 1872 4264 1872 Size: 2392 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3549 4201 5041 5942 6204 820 Size: 197 926 1606 652 840 901 262 1553 Fragments arranged by size: 1606 1553 926 901 840 652 262 197 SnaBI TAC'GTA Cuts at: 493 4728 493 Size: 4235 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6937 SphI G_CATG'C Cuts at: 1132 2023 4295 1132 Size: 891 2272 3774 Fragments arranged by size: 3774 2272 891 SspI AAT'ATT Cuts at: 5 52 4861 5 Size: 47 4809 2081 Fragments arranged by size: 4809 2081 47 StuI AGG'CCT Cuts at: 1517 2390 1517 Size: 873 6064 StyI C'CwwG_G Cuts at: 513 1065 2004 2466 2607 4376 513 Size: 552 939 462 141 1769 3074 Fragments arranged by size: 3074 1769 939 552 462 141 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4964 4981 5134 5319 5764 325 Size: 1255 3384 17 153 185 445 1498 Fragments arranged by size: 3384 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3383 5183 6895 Size: 274 80 33 51 1443 1406 1800 1712 Cuts at: 6895 96 Size: 138 Fragments arranged by size: 1800 1712 1443 1406 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2938 2970 6897 6929 2938 Size: 32 3927 32 2946 Fragments arranged by size: 3927 2946 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6937 XbaI T'CTAG_A Cuts at: 2671 3556 4273 2671 Size: 885 717 5335 Fragments arranged by size: 5335 885 717 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3388 3388 Size: 6937 XhoI C'TCGA_G Cuts at: 1943 3549 1943 Size: 1606 5331 XmaI C'CCGG_G Cuts at: 1870 4262 1870 Size: 2392 4545 XmnI GAAnn'nnTTC Cuts at: 2367 5066 2367 Size: 2699 4238 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4745 276 Size: 53 83 186 936 3211 2468 Fragments arranged by size: 3211 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspOMI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI