(Circular) (Six-base) MAPSORT of: pFN21AB4846.seq Check: 206 from: 1 to: 6145 pFN21AB4846 6145 bp With 182 enzymes: SgfI * February 27, 2009 14:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2097 3955 278 Size: 53 83 186 936 561 1858 2468 Fragments arranged by size: 2468 1858 936 561 186 83 53 AccI GT'mk_AC Cuts at: 1937 2962 3488 1937 Size: 1025 526 4594 Fragments arranged by size: 4594 1025 526 Acc65I G'GTAC_C Cuts at: 3466 3466 Size: 6145 AclI AA'CG_TT Cuts at: 1563 4272 4645 1563 Size: 2709 373 3063 Fragments arranged by size: 3063 2709 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 1995 4208 5592 5860 1632 Size: 363 2213 1384 268 1917 Fragments arranged by size: 2213 1917 1384 363 268 AfeI AGC'GCT Cuts at: 3272 3272 Size: 6145 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5948 AflIII A'CryG_T Cuts at: 1203 5044 6021 1203 Size: 3841 977 1327 Fragments arranged by size: 3841 1327 977 AgeI A'CCGG_T Cuts at: 4976 4976 Size: 6145 AhdI GACnn_n'nnGTC Cuts at: 4874 4874 Size: 6145 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6145 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6145 AlwNI CAG_nnn'CTG Cuts at: 3888 5460 3888 Size: 1572 4573 ApaI G_GGCC'C Cuts at: 2604 2604 Size: 6145 ApaLI G'TGCA_C Cuts at: 2891 4202 5358 2891 Size: 1311 1156 3678 Fragments arranged by size: 3678 1311 1156 ApoI r'AATT_y Cuts at: 1493 3454 3680 3832 5941 1493 Size: 1961 226 152 2109 1697 Fragments arranged by size: 2109 1961 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4699 160 Size: 872 3667 1606 Fragments arranged by size: 3667 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6145 AvaI C'yCGr_G Cuts at: 1870 1943 2260 2604 2651 3088 3470 1870 Size: 73 317 344 47 437 382 4545 Fragments arranged by size: 4545 437 382 344 317 73 47 AvrII C'CTAG_G Cuts at: 2365 2365 Size: 6145 BamHI G'GATC_C Cuts at: 2870 3475 3842 2870 Size: 605 367 5173 Fragments arranged by size: 5173 605 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2180 2974 3056 3227 3403 Size: 325 217 627 393 794 82 171 176 Cuts at: 3403 3466 4921 618 Size: 63 1455 1842 Fragments arranged by size: 1842 1455 794 627 393 325 217 176 171 82 63 BanII G_rGCy'C Cuts at: 1391 2150 2604 3122 3464 1391 Size: 759 454 518 342 4072 Fragments arranged by size: 4072 759 518 454 342 BbeI G_GCGC'C Cuts at: 2184 3060 3231 3407 2184 Size: 876 171 176 4922 Fragments arranged by size: 4922 876 176 171 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2533 2623 928 Size: 971 634 90 4450 Fragments arranged by size: 4450 971 634 90 BbvCI CC'TCA_GC Cuts at: 2216 2787 2216 Size: 571 5574 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3082 3116 4336 4370 1903 Size: 34 1145 34 1220 34 3678 Fragments arranged by size: 3678 1220 1145 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2803 4037 5247 2803 Size: 1234 1210 3701 Fragments arranged by size: 3701 1234 1210 BclI T'GATC_A Cuts at: 1548 2638 1548 Size: 1090 5055 BfrBI ATG'CAT Cuts at: 2067 2067 Size: 6145 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4756 136 Size: 107 122 71 631 605 3084 1525 Fragments arranged by size: 3084 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6140 6140 Size: 6145 BlpI GC'TnA_GC Cuts at: 2144 2430 3562 2144 Size: 286 1132 4727 Fragments arranged by size: 4727 1132 286 Bme1580I G_kGCm'C Cuts at: 1790 2604 2895 4206 5362 1790 Size: 814 291 1311 1156 2573 Fragments arranged by size: 2573 1311 1156 814 291 BmgBI CAC'GTC Cuts at: 1318 2921 3358 1318 Size: 1603 437 4105 Fragments arranged by size: 4105 1603 437 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3876 4834 442 Size: 416 53 83 399 24 2459 958 1753 Fragments arranged by size: 2459 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6145 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2708 3440 4805 1239 Size: 1469 732 1365 2579 Fragments arranged by size: 2579 1469 1365 732 Bpu10I CC'TnA_GC Cuts at: 2216 2787 2216 Size: 571 5574 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4270 5135 5433 4270 Size: 865 298 4982 Fragments arranged by size: 4982 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4808 882 Size: 617 3309 2219 Fragments arranged by size: 3309 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3936 493 Size: 711 2732 2702 Fragments arranged by size: 2732 2702 711 BsaBI GATnn'nnATC Cuts at: 6139 6139 Size: 6145 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2094 2168 2181 2190 Size: 53 83 186 936 561 74 13 9 Cuts at: 2190 3057 3077 3228 3404 3952 4334 4969 275 Size: 867 20 151 176 548 382 635 1451 Fragments arranged by size: 1451 936 867 635 561 548 382 186 176 151 83 74 53 20 13 9 BsaWI w'CCGG_w Cuts at: 4578 4976 5250 5397 5808 4578 Size: 398 274 147 411 4915 Fragments arranged by size: 4915 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3236 3266 3371 3401 3416 Size: 30 165 30 1833 30 105 30 15 Cuts at: 3416 3446 1178 Size: 30 3877 Fragments arranged by size: 3877 1833 165 105 30 30 30 30 30 15 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2233 3437 1186 Size: 1047 1204 3894 Fragments arranged by size: 3894 1204 1047 BseYI C'CCAG_C Cuts at: 2176 2731 2896 3335 5348 5844 2176 Size: 555 165 439 2013 496 2477 Fragments arranged by size: 2477 2013 555 496 439 165 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3283 1163 Size: 573 1547 4025 Fragments arranged by size: 4025 1547 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2230 2500 3237 3595 4356 5384 Size: 275 186 229 270 737 358 761 1028 Cuts at: 5384 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 737 358 275 270 229 186 BsiHKAI G_wGCw'C Cuts at: 2895 4206 4291 5362 2895 Size: 1311 85 1071 3678 Fragments arranged by size: 3678 1311 1071 85 BsmI GAATG_Cn' Cuts at: 2069 3656 3749 2069 Size: 1587 93 4465 Fragments arranged by size: 4465 1587 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2085 2085 Size: 6145 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2150 2604 2895 3122 3464 4206 4291 Size: 399 360 454 291 227 342 742 85 Cuts at: 4291 5362 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 454 399 360 342 291 227 85 BspEI T'CCGG_A Cuts at: 5808 5808 Size: 6145 BspHI T'CATG_A Cuts at: 2055 4034 5764 2055 Size: 1979 1730 2436 Fragments arranged by size: 2436 1979 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3153 3500 844 Size: 2309 347 3489 Fragments arranged by size: 3489 2309 347 BsrBI CCG'CTC Cuts at: 1394 1823 2223 2674 4032 1394 Size: 429 400 451 1358 3507 Fragments arranged by size: 3507 1358 451 429 400 BsrDI GCAATG_nn' Cuts at: 65 1248 2416 2874 4640 4814 65 Size: 1183 1168 458 1766 174 1396 Fragments arranged by size: 1766 1396 1183 1168 458 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2192 2207 3079 3236 3314 4789 4976 Size: 110 382 15 872 157 78 1475 187 Cuts at: 4976 1700 Size: 2869 Fragments arranged by size: 2869 1475 872 382 187 157 110 78 15 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6145 BssHII G'CGCG_C Cuts at: 1925 3413 1925 Size: 1488 4657 BssSI C'ACGA_G Cuts at: 2110 2751 4205 5217 2110 Size: 641 1454 1012 3038 Fragments arranged by size: 3038 1454 1012 641 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6145 BstBI TT'CG_AA Cuts at: 4949 4949 Size: 6145 BstEII G'GTnAC_C Cuts at: 1184 3082 1184 Size: 1898 4247 BstXI CCAn_nnnn'nTGG Cuts at: 3400 3400 Size: 6145 BstYI r'GATC_y Cuts at: 1972 2820 2870 3475 3842 4227 4244 5696 5782 Size: 848 50 605 367 385 17 1452 86 Cuts at: 5782 5794 6140 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 848 605 385 367 346 86 50 17 12 Bsu36I CC'TnA_GG Cuts at: 2486 2486 Size: 6145 BtgI C'CryG_G Cuts at: 513 1065 2004 2837 3114 3255 513 Size: 552 939 833 277 141 3403 Fragments arranged by size: 3403 939 833 552 277 141 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2329 487 Size: 683 56 1103 4303 Fragments arranged by size: 4303 1103 683 56 BtsI GCAGTG_nn' Cuts at: 2772 3661 4455 4475 2772 Size: 889 794 20 4442 Fragments arranged by size: 4442 889 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6145 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6110 DraI TTT'AAA Cuts at: 3449 3449 Size: 6145 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5152 809 Size: 507 3836 1802 Fragments arranged by size: 3836 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2205 2250 3111 Size: 54 1425 50 275 18 375 45 861 Cuts at: 3111 3123 3234 3592 4481 6110 8 Size: 12 111 358 889 1629 43 Fragments arranged by size: 1629 1425 889 861 375 358 275 111 54 50 45 43 18 12 EagI C'GGCC_G Cuts at: 1537 1812 3234 3592 1537 Size: 275 1422 358 4090 Fragments arranged by size: 4090 1422 358 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2503 4075 1353 Size: 1150 1572 3423 Fragments arranged by size: 3423 1572 1150 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4732 5106 5252 387 Size: 1064 138 3143 374 146 1280 Fragments arranged by size: 3143 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 1995 2708 3440 4208 4805 5592 5860 Size: 393 363 713 732 768 597 787 268 Cuts at: 5860 1239 Size: 1524 Fragments arranged by size: 1524 787 768 732 713 597 393 363 268 EcoNI CCTnn'n_nnAGG Cuts at: 3422 3422 Size: 6145 EcoO109I rG'GnC_Cy Cuts at: 2601 3438 2601 Size: 837 5308 EcoRI G'AATT_C Cuts at: 3454 3832 3454 Size: 378 5767 FspI TGC'GCA Cuts at: 2276 4651 2276 Size: 2375 3770 FspAI rTGC'GCAy Cuts at: 2276 2276 Size: 6145 HaeII r_GCGC'y Cuts at: 1678 2184 2199 2326 3060 3231 3274 3407 5292 Size: 506 15 127 734 171 43 133 1885 Cuts at: 5292 1678 Size: 2531 Fragments arranged by size: 2531 1885 734 506 171 133 127 43 15 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4786 4818 4860 4892 1373 Size: 32 135 32 3214 32 42 32 2626 Fragments arranged by size: 3214 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2963 3489 3735 669 Size: 988 281 1025 526 246 3079 Fragments arranged by size: 3079 1025 988 526 281 246 HindIII A'AGCT_T Cuts at: 748 2518 748 Size: 1770 4375 HpaI GTT'AAC Cuts at: 3735 3735 Size: 6145 KasI G'GCGC_C Cuts at: 2180 3056 3227 3403 2180 Size: 876 171 176 4922 Fragments arranged by size: 4922 876 176 171 KpnI G_GTAC'C Cuts at: 3470 3470 Size: 6145 MfeI C'AATT_G Cuts at: 3744 3744 Size: 6145 MluI A'CGCG_T Cuts at: 6021 6021 Size: 6145 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2848 3057 5259 5443 1305 Size: 1543 209 2202 184 2007 Fragments arranged by size: 2202 2007 1543 209 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4323 Fragments arranged by size: 4323 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2399 2708 3104 3398 4103 4462 4621 Size: 648 1233 309 396 294 705 359 159 Cuts at: 4621 518 Size: 2042 Fragments arranged by size: 2042 1233 705 648 396 359 309 294 159 MspA1I CmG'CkG Cuts at: 1606 2176 2695 2928 3561 4238 5386 5631 1606 Size: 570 519 233 633 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 633 570 519 245 233 NaeI GCC'GGC Cuts at: 2194 2209 3238 2194 Size: 15 1029 5101 Fragments arranged by size: 5101 1029 15 NarI GG'CG_CC Cuts at: 2181 3057 3228 3404 2181 Size: 876 171 176 4922 Fragments arranged by size: 4922 876 176 171 NcoI C'CATG_G Cuts at: 513 1065 2004 3114 3255 513 Size: 552 939 1110 141 3403 Fragments arranged by size: 3403 1110 939 552 141 NdeI CA'TA_TG Cuts at: 387 387 Size: 6145 NgoMIV G'CCGG_C Cuts at: 2192 2207 3236 2192 Size: 15 1029 5101 Fragments arranged by size: 5101 1029 15 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6145 NotI GC'GGCC_GC Cuts at: 3592 3592 Size: 6145 NsiI A_TGCA'T Cuts at: 2069 2069 Size: 6145 NspI r_CATG'y Cuts at: 1132 1229 3046 3109 3503 5048 1132 Size: 97 1817 63 394 1545 2229 Fragments arranged by size: 2229 1817 1545 394 97 63 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6145 PciI A'CATG_T Cuts at: 5044 5044 Size: 6145 PflMI CCAn_nnn'nTGG Cuts at: 1108 2866 1108 Size: 1758 4387 PmeI GTTT'AAAC Cuts at: 3449 3449 Size: 6145 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4214 4246 5727 5759 4214 Size: 32 1481 32 4600 Fragments arranged by size: 4600 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3080 1613 Size: 1467 4678 PsiI TTA'TAA Cuts at: 3715 3715 Size: 6145 PspOMI G'GGCC_C Cuts at: 2600 2600 Size: 6145 PstI C_TGCA'G Cuts at: 830 2950 3497 830 Size: 2120 547 3478 Fragments arranged by size: 3478 2120 547 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6145 PvuII CAG'CTG Cuts at: 2695 2695 Size: 6145 SalI G'TCGA_C Cuts at: 1936 2961 3487 1936 Size: 1025 526 4594 Fragments arranged by size: 4594 1025 526 SbfI CC_TGCA'GG Cuts at: 3497 3497 Size: 6145 ScaI AGT'ACT Cuts at: 4393 6105 4393 Size: 1712 4433 SexAI A'CCwGG_T Cuts at: 2463 2463 Size: 6145 SfcI C'TryA_G Cuts at: 826 1045 2946 3493 4628 5309 5500 826 Size: 219 1901 547 1135 681 191 1471 Fragments arranged by size: 1901 1471 1135 681 547 219 191 SfoI GGC'GCC Cuts at: 2182 3058 3229 3405 2182 Size: 876 171 176 4922 Fragments arranged by size: 4922 876 176 171 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6145 SgrAI Cr'CCGG_yG Cuts at: 1700 2192 1700 Size: 492 5653 SmaI CCC'GGG Cuts at: 1872 2262 2653 3472 1872 Size: 390 391 819 4545 Fragments arranged by size: 4545 819 391 390 SmlI C'TyrA_G Cuts at: 820 1017 1943 4249 5150 5412 820 Size: 197 926 2306 901 262 1553 Fragments arranged by size: 2306 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3936 493 Size: 3443 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6145 SphI G_CATG'C Cuts at: 1132 3046 3503 1132 Size: 1914 457 3774 Fragments arranged by size: 3774 1914 457 SspI AAT'ATT Cuts at: 5 52 4069 5 Size: 47 4017 2081 Fragments arranged by size: 4017 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6145 StyI C'CwwG_G Cuts at: 513 1065 2004 2365 3114 3255 3584 513 Size: 552 939 361 749 141 329 3074 Fragments arranged by size: 3074 939 749 552 361 329 141 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2486 2745 2910 4172 4189 4342 4527 Size: 1255 906 259 165 1262 17 153 185 Cuts at: 4527 4972 325 Size: 445 1498 Fragments arranged by size: 1498 1262 1255 906 445 259 185 165 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3219 4391 6103 Size: 274 80 33 51 1443 1242 1172 1712 Cuts at: 6103 96 Size: 138 Fragments arranged by size: 1712 1443 1242 1172 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3371 3403 3416 3448 6105 6137 3371 Size: 32 13 32 2657 32 3379 Fragments arranged by size: 3379 2657 32 32 32 13 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6145 XbaI T'CTAG_A Cuts at: 2087 3481 2087 Size: 1394 4751 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2835 2835 Size: 6145 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6145 XmaI C'CCGG_G Cuts at: 1870 2260 2651 3470 1870 Size: 390 391 819 4545 Fragments arranged by size: 4545 819 391 390 XmnI GAAnn'nnTTC Cuts at: 2519 4274 2519 Size: 1755 4390 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2095 3953 276 Size: 53 83 186 936 561 1858 2468 Fragments arranged by size: 2468 1858 936 561 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI BaeI BplI BsiWI BstZ17I DraIII EcoICRI EcoRV FalI FseI NruI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SrfI SwaI