(Circular) (Six-base) MAPSORT of: pFN21AB4784.seq Check: 9863 from: 1 to: 5518 pFN21AB4784 5518 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2787 3328 278 Size: 53 83 186 936 1251 541 2468 Fragments arranged by size: 2468 1251 936 541 186 83 53 AccI GT'mk_AC Cuts at: 1937 2787 2861 1937 Size: 850 74 4594 Fragments arranged by size: 4594 850 74 Acc65I G'GTAC_C Cuts at: 2839 2839 Size: 5518 AclI AA'CG_TT Cuts at: 1563 3645 4018 1563 Size: 2082 373 3063 Fragments arranged by size: 3063 2082 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3581 4965 5233 1632 Size: 1949 1384 268 1917 Fragments arranged by size: 1949 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5321 AflIII A'CryG_T Cuts at: 1203 4417 5394 1203 Size: 3214 977 1327 Fragments arranged by size: 3214 1327 977 AgeI A'CCGG_T Cuts at: 2538 4349 2538 Size: 1811 3707 AhdI GACnn_n'nnGTC Cuts at: 4247 4247 Size: 5518 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5518 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5518 AlwNI CAG_nnn'CTG Cuts at: 2699 2732 3261 4833 2699 Size: 33 529 1572 3384 Fragments arranged by size: 3384 1572 529 33 ApaI G_GGCC'C Cuts at: 2560 2815 2560 Size: 255 5263 ApaLI G'TGCA_C Cuts at: 3575 4731 3575 Size: 1156 4362 ApoI r'AATT_y Cuts at: 1493 2827 3053 3205 5314 1493 Size: 1334 226 152 2109 1697 Fragments arranged by size: 2109 1697 1334 226 152 AseI AT'TA_AT Cuts at: 160 1032 4072 160 Size: 872 3040 1606 Fragments arranged by size: 3040 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5518 AvaI C'yCGr_G Cuts at: 1870 1943 2684 2843 1870 Size: 73 741 159 4545 Fragments arranged by size: 4545 741 159 73 BamHI G'GATC_C Cuts at: 2848 3215 2848 Size: 367 5151 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2839 4294 618 Size: 325 217 627 1052 1455 1842 Fragments arranged by size: 1842 1455 1052 627 325 217 BanII G_rGCy'C Cuts at: 1391 2181 2560 2815 2837 1391 Size: 790 379 255 22 4072 Fragments arranged by size: 4072 790 379 255 22 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2049 928 Size: 971 150 4397 Fragments arranged by size: 4397 971 150 BbvCI CC'TCA_GC Cuts at: 2612 2612 Size: 5518 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3709 3743 1903 Size: 34 1772 34 3678 Fragments arranged by size: 3678 1772 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3410 4620 3410 Size: 1210 4308 BclI T'GATC_A Cuts at: 1548 1548 Size: 5518 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2501 4129 136 Size: 107 122 71 631 605 829 1628 1525 Fragments arranged by size: 1628 1525 829 631 605 122 107 71 BglII A'GATC_T Cuts at: 5513 5513 Size: 5518 BlpI GC'TnA_GC Cuts at: 2768 2935 2768 Size: 167 5351 Bme1580I G_kGCm'C Cuts at: 1790 2560 2815 3579 4735 1790 Size: 770 255 764 1156 2573 Fragments arranged by size: 2573 1156 770 764 255 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5518 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3249 4207 442 Size: 416 53 83 399 24 1832 958 1753 Fragments arranged by size: 1832 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5518 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2213 2222 4178 1239 Size: 974 9 1956 2579 Fragments arranged by size: 2579 1956 974 9 Bpu10I CC'TnA_GC Cuts at: 2612 2612 Size: 5518 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2411 3643 4508 4806 2411 Size: 1232 865 298 3123 Fragments arranged by size: 3123 1232 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2080 4181 882 Size: 617 581 2101 2219 Fragments arranged by size: 2219 2101 617 581 BsaAI yAC'GTr Cuts at: 493 1204 3309 493 Size: 711 2105 2702 Fragments arranged by size: 2702 2105 711 BsaBI GATnn'nnATC Cuts at: 2753 5512 2753 Size: 2759 2759 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2046 2784 3325 3707 Size: 53 83 186 936 513 738 541 382 Cuts at: 3707 4342 275 Size: 635 1451 Fragments arranged by size: 1451 936 738 635 541 513 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2538 3951 4349 4623 4770 5181 2538 Size: 1413 398 274 147 411 2875 Fragments arranged by size: 2875 1413 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5293 Fragments arranged by size: 5293 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2777 1186 Size: 1591 3927 BseYI C'CCAG_C Cuts at: 2145 4721 5217 2145 Size: 2576 496 2446 Fragments arranged by size: 2576 2446 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2723 1163 Size: 573 987 3958 Fragments arranged by size: 3958 987 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2968 3729 4757 1540 Size: 275 186 967 761 1028 2301 Fragments arranged by size: 2301 1028 967 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2058 2181 2427 3579 3664 4735 2058 Size: 123 246 1152 85 1071 2841 Fragments arranged by size: 2841 1152 1071 246 123 85 BsmI GAATG_Cn' Cuts at: 2695 3029 3122 2695 Size: 334 93 5091 Fragments arranged by size: 5091 334 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2449 2449 Size: 5518 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2058 2181 2427 2560 2815 2837 3579 Size: 399 268 123 246 133 255 22 742 Cuts at: 3579 3664 4735 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 268 255 246 133 123 85 22 BspEI T'CCGG_A Cuts at: 5181 5181 Size: 5518 BspHI T'CATG_A Cuts at: 3407 5137 3407 Size: 1730 3788 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2276 2538 2873 844 Size: 1432 262 335 3489 Fragments arranged by size: 3489 1432 335 262 BsrBI CCG'CTC Cuts at: 1394 1823 2772 3405 1394 Size: 429 949 633 3507 Fragments arranged by size: 3507 949 633 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2208 4013 4187 65 Size: 1183 960 1805 174 1396 Fragments arranged by size: 1805 1396 1183 960 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2538 4162 4349 1700 Size: 110 728 1624 187 2869 Fragments arranged by size: 2869 1624 728 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5518 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5518 BssSI C'ACGA_G Cuts at: 2100 3578 4590 2100 Size: 1478 1012 3028 Fragments arranged by size: 3028 1478 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5518 BstBI TT'CG_AA Cuts at: 4322 4322 Size: 5518 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5518 BstXI CCAn_nnnn'nTGG Cuts at: 2153 2153 Size: 5518 BstYI r'GATC_y Cuts at: 1972 2221 2256 2307 2848 3215 3600 3617 5069 Size: 249 35 51 541 367 385 17 1452 Cuts at: 5069 5155 5167 5513 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 541 385 367 346 249 86 51 35 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4027 Fragments arranged by size: 4027 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4779 Fragments arranged by size: 4779 683 56 BtsI GCAGTG_nn' Cuts at: 2490 2700 3034 3828 3848 2490 Size: 210 334 794 20 4160 Fragments arranged by size: 4160 794 334 210 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5518 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5483 DraI TTT'AAA Cuts at: 2822 2822 Size: 5518 DraIII CAC_nnn'GTG Cuts at: 2416 2416 Size: 5518 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4525 809 Size: 507 3209 1802 Fragments arranged by size: 3209 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2155 2502 2965 Size: 54 1425 50 275 18 325 347 463 Cuts at: 2965 3854 5483 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 463 347 325 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2965 1537 Size: 275 1153 4090 Fragments arranged by size: 4090 1153 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2492 3448 1353 Size: 1139 956 3423 Fragments arranged by size: 3423 1139 956 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4105 4479 4625 387 Size: 1064 138 2516 374 146 1280 Fragments arranged by size: 2516 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2179 2179 Size: 5518 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2213 2222 3581 4178 4965 5233 1239 Size: 393 581 9 1359 597 787 268 1524 Fragments arranged by size: 1524 1359 787 597 581 393 268 9 EcoO109I rG'GnC_Cy Cuts at: 2122 2265 2608 2629 2812 2122 Size: 143 343 21 183 4828 Fragments arranged by size: 4828 343 183 143 21 EcoRI G'AATT_C Cuts at: 2827 3205 2827 Size: 378 5140 FspI TGC'GCA Cuts at: 4024 4024 Size: 5518 HaeII r_GCGC'y Cuts at: 1678 4665 1678 Size: 2987 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2097 2129 4159 4191 4233 Size: 32 135 32 525 32 2030 32 42 Cuts at: 4233 4265 1373 Size: 32 2626 Fragments arranged by size: 2626 2030 525 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2862 3108 669 Size: 988 281 924 246 3079 Fragments arranged by size: 3079 988 924 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5518 HpaI GTT'AAC Cuts at: 3108 3108 Size: 5518 KpnI G_GTAC'C Cuts at: 2843 2843 Size: 5518 MfeI C'AATT_G Cuts at: 3117 3117 Size: 5518 MluI A'CGCG_T Cuts at: 5394 5394 Size: 5518 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2199 4632 4816 1305 Size: 894 2433 184 2007 Fragments arranged by size: 2433 2007 894 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2157 2504 10 Size: 54 1425 343 325 347 3024 Fragments arranged by size: 3024 1425 347 343 325 54 MslI CAynn'nnrTG Cuts at: 518 1166 3476 3835 3994 518 Size: 648 2310 359 159 2042 Fragments arranged by size: 2310 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2806 2934 3611 4759 5004 1606 Size: 1200 128 677 1148 245 2120 Fragments arranged by size: 2120 1200 1148 677 245 128 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4027 Fragments arranged by size: 4027 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5518 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5518 NotI GC'GGCC_GC Cuts at: 2965 2965 Size: 5518 NspI r_CATG'y Cuts at: 1132 1229 2153 2876 4421 1132 Size: 97 924 723 1545 2229 Fragments arranged by size: 2229 1545 924 723 97 PasI CC'CwG_GG Cuts at: 1348 2261 1348 Size: 913 4605 PciI A'CATG_T Cuts at: 4417 4417 Size: 5518 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5518 PfoI T'CCnGG_A Cuts at: 2649 2649 Size: 5518 PmeI GTTT'AAAC Cuts at: 2822 2822 Size: 5518 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3587 3619 5100 5132 3587 Size: 32 1481 32 3973 Fragments arranged by size: 3973 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2265 2608 2629 2265 Size: 343 21 5154 Fragments arranged by size: 5154 343 21 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5518 PsiI TTA'TAA Cuts at: 3088 3088 Size: 5518 PspOMI G'GGCC_C Cuts at: 2556 2811 2556 Size: 255 5263 PstI C_TGCA'G Cuts at: 830 2870 830 Size: 2040 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5518 PvuII CAG'CTG Cuts at: 2806 2806 Size: 5518 SacI G_AGCT'C Cuts at: 2181 2181 Size: 5518 SalI G'TCGA_C Cuts at: 1936 2860 1936 Size: 924 4594 SbfI CC_TGCA'GG Cuts at: 2870 2870 Size: 5518 ScaI AGT'ACT Cuts at: 3766 5478 3766 Size: 1712 3806 SexAI A'CCwGG_T Cuts at: 2412 2412 Size: 5518 SfcI C'TryA_G Cuts at: 826 1045 2388 2866 4001 4682 4873 826 Size: 219 1343 478 1135 681 191 1471 Fragments arranged by size: 1471 1343 1135 681 478 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5518 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5518 SmaI CCC'GGG Cuts at: 1872 2845 1872 Size: 973 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2426 3622 4523 4785 820 Size: 197 926 483 1196 901 262 1553 Fragments arranged by size: 1553 1196 926 901 483 262 197 SnaBI TAC'GTA Cuts at: 493 3309 493 Size: 2816 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5518 SphI G_CATG'C Cuts at: 1132 2876 1132 Size: 1744 3774 SspI AAT'ATT Cuts at: 5 52 3442 5 Size: 47 3390 2081 Fragments arranged by size: 3390 2081 47 StuI AGG'CCT Cuts at: 1517 2070 2434 1517 Size: 553 364 4601 Fragments arranged by size: 4601 553 364 StyI C'CwwG_G Cuts at: 513 1065 2004 2797 2957 513 Size: 552 939 793 160 3074 Fragments arranged by size: 3074 939 793 552 160 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2042 3545 3562 3715 3900 4345 325 Size: 1255 462 1503 17 153 185 445 1498 Fragments arranged by size: 1503 1498 1255 462 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3764 5476 96 Size: 274 80 33 51 1443 1787 1712 138 Fragments arranged by size: 1787 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5478 5510 5478 Size: 32 5486 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5518 XbaI T'CTAG_A Cuts at: 2854 2854 Size: 5518 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2068 2068 Size: 5518 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5518 XmaI C'CCGG_G Cuts at: 1870 2843 1870 Size: 973 4545 XmnI GAAnn'nnTTC Cuts at: 2481 3647 2481 Size: 1166 4352 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2785 3326 276 Size: 53 83 186 936 1251 541 2468 Fragments arranged by size: 2468 1251 936 541 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI