(Circular) (Six-base) MAPSORT of: pFN21AB4618.seq Check: 7843 from: 1 to: 6943 pFN21AB4618 6943 bp With 182 enzymes: SgfI * February 27, 2009 14:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4753 278 Size: 53 83 186 936 3217 2468 Fragments arranged by size: 3217 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3911 4286 1937 Size: 1974 375 4594 Fragments arranged by size: 4594 1974 375 Acc65I G'GTAC_C Cuts at: 4264 4264 Size: 6943 AclI AA'CG_TT Cuts at: 1563 5070 5443 1563 Size: 3507 373 3063 Fragments arranged by size: 3507 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3168 3531 4116 4235 5006 6390 6658 1632 Size: 1536 363 585 119 771 1384 268 1917 Fragments arranged by size: 1917 1536 1384 771 585 363 268 119 AfeI AGC'GCT Cuts at: 2984 2984 Size: 6943 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6746 AflIII A'CryG_T Cuts at: 1203 2352 2354 2658 5842 6819 1203 Size: 1149 2 304 3184 977 1327 Fragments arranged by size: 3184 1327 1149 977 304 2 AgeI A'CCGG_T Cuts at: 3771 5774 3771 Size: 2003 4940 AhdI GACnn_n'nnGTC Cuts at: 5672 5672 Size: 6943 AleI CACnn'nnGTG Cuts at: 1166 3512 1166 Size: 2346 4597 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6943 AlwNI CAG_nnn'CTG Cuts at: 3686 4686 6258 3686 Size: 1000 1572 4371 Fragments arranged by size: 4371 1572 1000 ApaI G_GGCC'C Cuts at: 2719 2719 Size: 6943 ApaLI G'TGCA_C Cuts at: 4057 5000 6156 4057 Size: 943 1156 4844 Fragments arranged by size: 4844 1156 943 ApoI r'AATT_y Cuts at: 1493 3761 4040 4252 4478 4630 6739 1493 Size: 2268 279 212 226 152 2109 1697 Fragments arranged by size: 2268 2109 1697 279 226 212 152 AseI AT'TA_AT Cuts at: 160 1032 5497 160 Size: 872 4465 1606 Fragments arranged by size: 4465 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6943 AvaI C'yCGr_G Cuts at: 1870 1943 2469 3787 4268 1870 Size: 73 526 1318 481 4545 Fragments arranged by size: 4545 1318 526 481 73 BamHI G'GATC_C Cuts at: 4273 4640 4273 Size: 367 6576 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2011 3523 4264 5719 618 Size: 325 217 627 224 1512 741 1455 1842 Fragments arranged by size: 1842 1512 1455 741 627 325 224 217 BanII G_rGCy'C Cuts at: 1391 2719 3061 4262 1391 Size: 1328 342 1201 4072 Fragments arranged by size: 4072 1328 1201 342 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2150 2995 4129 928 Size: 971 251 845 1134 3742 Fragments arranged by size: 3742 1134 971 845 251 BbvCI CC'TCA_GC Cuts at: 2802 2802 Size: 6943 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5134 5168 1903 Size: 34 3197 34 3678 Fragments arranged by size: 3678 3197 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4835 6045 4835 Size: 1210 5733 BclI T'GATC_A Cuts at: 1548 1548 Size: 6943 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2723 5554 136 Size: 107 122 71 631 605 1051 2831 1525 Fragments arranged by size: 2831 1525 1051 631 605 122 107 71 BglII A'GATC_T Cuts at: 6938 6938 Size: 6943 BlpI GC'TnA_GC Cuts at: 4360 4360 Size: 6943 Bme1580I G_kGCm'C Cuts at: 1790 2719 3528 4061 5004 6160 1790 Size: 929 809 533 943 1156 2573 Fragments arranged by size: 2573 1156 943 929 809 533 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6943 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3522 3882 3952 Size: 416 53 83 399 24 2105 360 70 Cuts at: 3952 4674 5632 442 Size: 722 958 1753 Fragments arranged by size: 2105 1753 958 722 416 399 360 83 70 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6943 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2688 2888 3561 5603 1239 Size: 1449 200 673 2042 2579 Fragments arranged by size: 2579 2042 1449 673 200 Bpu10I CC'TnA_GC Cuts at: 2802 2802 Size: 6943 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3432 4139 5068 5933 6231 3432 Size: 707 929 865 298 4144 Fragments arranged by size: 4144 929 865 707 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2873 5606 882 Size: 617 1374 2733 2219 Fragments arranged by size: 2733 2219 1374 617 BsaAI yAC'GTr Cuts at: 493 1204 2355 2431 2745 4734 493 Size: 711 1151 76 314 1989 2702 Fragments arranged by size: 2702 1989 1151 711 314 76 BsaBI GATnn'nnATC Cuts at: 2367 6937 2367 Size: 4570 2373 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2445 4750 5132 5767 Size: 53 83 186 936 912 2305 382 635 Cuts at: 5767 275 Size: 1451 Fragments arranged by size: 2305 1451 936 912 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3336 3771 5376 5774 6048 6195 6606 3336 Size: 435 1605 398 274 147 411 3673 Fragments arranged by size: 3673 1605 435 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2450 2480 2910 2940 3408 Size: 30 165 30 1047 30 430 30 468 Cuts at: 3408 3438 1178 Size: 30 4683 Fragments arranged by size: 4683 1047 468 430 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6943 BseYI C'CCAG_C Cuts at: 2613 3064 6146 6642 2613 Size: 451 3082 496 2914 Fragments arranged by size: 3082 2914 496 451 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6370 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3481 4393 5154 6182 1540 Size: 275 186 1480 912 761 1028 2301 Fragments arranged by size: 2301 1480 1028 912 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3061 3870 4061 5004 5089 6160 3061 Size: 809 191 943 85 1071 3844 Fragments arranged by size: 3844 1071 943 809 191 85 BsmI GAATG_Cn' Cuts at: 2352 2775 4454 4547 2352 Size: 423 1679 93 4748 Fragments arranged by size: 4748 1679 423 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2452 2452 Size: 6943 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2719 3061 3528 3870 4061 4262 5004 Size: 399 929 342 467 342 191 201 742 Cuts at: 5004 5089 6160 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 929 742 467 399 342 342 201 191 85 BspEI T'CCGG_A Cuts at: 6606 6606 Size: 6943 BspHI T'CATG_A Cuts at: 4832 6562 4832 Size: 1730 5213 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4298 844 Size: 3454 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2326 4830 1394 Size: 429 503 2504 3507 Fragments arranged by size: 3507 2504 503 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2199 3117 3303 5438 5612 65 Size: 1183 951 918 186 2135 174 1396 Fragments arranged by size: 2135 1396 1183 951 918 186 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2287 2754 3771 5587 5774 1700 Size: 110 477 467 1017 1816 187 2869 Fragments arranged by size: 2869 1816 1017 477 467 187 110 BsrGI T'GTAC_A Cuts at: 96 3207 4188 96 Size: 3111 981 2851 Fragments arranged by size: 3111 2851 981 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6943 BssSI C'ACGA_G Cuts at: 5003 6015 5003 Size: 1012 5931 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6943 BstBI TT'CG_AA Cuts at: 5747 5747 Size: 6943 BstEII G'GTnAC_C Cuts at: 1184 3782 4024 1184 Size: 2598 242 4103 Fragments arranged by size: 4103 2598 242 BstXI CCAn_nnnn'nTGG Cuts at: 3310 3310 Size: 6943 BstYI r'GATC_y Cuts at: 1972 2332 2809 3339 4273 4640 5025 5042 6494 Size: 360 477 530 934 367 385 17 1452 Cuts at: 6494 6580 6592 6938 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 934 530 477 385 367 360 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3912 3912 Size: 6943 BtgI C'CryG_G Cuts at: 513 1065 2004 2036 2834 3861 513 Size: 552 939 32 798 1027 3595 Fragments arranged by size: 3595 1027 939 798 552 32 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2621 3099 487 Size: 683 56 1395 478 4331 Fragments arranged by size: 4331 1395 683 478 56 BtsI GCAGTG_nn' Cuts at: 2579 4459 5253 5273 2579 Size: 1880 794 20 4249 Fragments arranged by size: 4249 1880 794 20 ClaI AT'CG_AT Cuts at: 1554 2559 1554 Size: 1005 5938 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6908 DraI TTT'AAA Cuts at: 4247 4247 Size: 6943 DraIII CAC_nnn'GTG Cuts at: 4099 4099 Size: 6943 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2444 5950 809 Size: 507 1128 3506 1802 Fragments arranged by size: 3506 1802 1128 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2033 2493 3312 Size: 54 1425 50 275 18 203 460 819 Cuts at: 3312 3693 3993 4390 5279 6908 8 Size: 381 300 397 889 1629 43 Fragments arranged by size: 1629 1425 889 819 460 397 381 300 275 203 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4390 1537 Size: 275 2578 4090 Fragments arranged by size: 4090 2578 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2026 2064 2180 3158 3425 3500 4211 4873 Size: 673 38 116 978 267 75 711 662 Cuts at: 4873 1353 Size: 3423 Fragments arranged by size: 3423 978 711 673 662 267 116 75 38 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5530 5904 6050 387 Size: 1064 138 3941 374 146 1280 Fragments arranged by size: 3941 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3059 3059 Size: 6943 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2688 2888 3168 3531 3561 4116 4235 Size: 393 1056 200 280 363 30 555 119 Cuts at: 4235 5006 5603 6390 6658 1239 Size: 771 597 787 268 1524 Fragments arranged by size: 1524 1056 787 771 597 555 393 363 280 268 200 119 30 EcoNI CCTnn'n_nnAGG Cuts at: 2957 2957 Size: 6943 EcoO109I rG'GnC_Cy Cuts at: 2120 2672 2715 2716 2890 2120 Size: 552 43 1 174 6173 Fragments arranged by size: 6173 552 174 43 1 EcoRI G'AATT_C Cuts at: 4252 4630 4252 Size: 378 6565 EcoRV GAT'ATC Cuts at: 3709 3934 3709 Size: 225 6718 FspI TGC'GCA Cuts at: 2585 5449 2585 Size: 2864 4079 HaeII r_GCGC'y Cuts at: 1678 2986 6090 1678 Size: 1308 3104 2531 Fragments arranged by size: 3104 2531 1308 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4135 4167 5584 5616 5658 Size: 32 135 32 2563 32 1417 32 42 Cuts at: 5658 5690 1373 Size: 32 2626 Fragments arranged by size: 2626 2563 1417 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4287 4533 669 Size: 988 281 2349 246 3079 Fragments arranged by size: 3079 2349 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6943 HpaI GTT'AAC Cuts at: 4533 4533 Size: 6943 KpnI G_GTAC'C Cuts at: 4268 4268 Size: 6943 MfeI C'AATT_G Cuts at: 4542 4542 Size: 6943 MluI A'CGCG_T Cuts at: 6819 6819 Size: 6943 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6057 6241 1305 Size: 4752 184 2007 Fragments arranged by size: 4752 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2035 2495 3314 3695 3995 Size: 54 1425 343 203 460 819 381 300 Cuts at: 3995 10 Size: 2958 Fragments arranged by size: 2958 1425 819 460 381 343 300 203 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2210 2561 3512 4901 5260 5419 Size: 648 843 201 351 951 1389 359 159 Cuts at: 5419 518 Size: 2042 Fragments arranged by size: 2042 1389 951 843 648 359 351 201 159 MspA1I CmG'CkG Cuts at: 1606 2801 3430 4359 5036 6184 6429 1606 Size: 1195 629 929 677 1148 245 2120 Fragments arranged by size: 2120 1195 1148 929 677 629 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3861 513 Size: 552 939 1857 3595 Fragments arranged by size: 3595 1857 939 552 NdeI CA'TA_TG Cuts at: 387 3349 3957 387 Size: 2962 608 3373 Fragments arranged by size: 3373 2962 608 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6943 NotI GC'GGCC_GC Cuts at: 4390 4390 Size: 6943 NspI r_CATG'y Cuts at: 1132 1229 2215 2662 4301 5846 1132 Size: 97 986 447 1639 1545 2229 Fragments arranged by size: 2229 1639 1545 986 447 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6943 PciI A'CATG_T Cuts at: 2658 5842 2658 Size: 3184 3759 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6943 PmeI GTTT'AAAC Cuts at: 4247 4247 Size: 6943 PmlI CAC'GTG Cuts at: 2355 2355 Size: 6943 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5012 5044 6525 6557 5012 Size: 32 1481 32 5398 Fragments arranged by size: 5398 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2120 2672 2890 2120 Size: 552 218 6173 Fragments arranged by size: 6173 552 218 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6943 PsiI TTA'TAA Cuts at: 2685 4513 2685 Size: 1828 5115 PspOMI G'GGCC_C Cuts at: 2715 2715 Size: 6943 PstI C_TGCA'G Cuts at: 830 3324 4295 830 Size: 2494 971 3478 Fragments arranged by size: 3478 2494 971 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6943 PvuII CAG'CTG Cuts at: 2801 2801 Size: 6943 SacI G_AGCT'C Cuts at: 3061 3061 Size: 6943 SalI G'TCGA_C Cuts at: 1936 4285 1936 Size: 2349 4594 SapI GCTCTTCn'nnn_ Cuts at: 2026 4211 2026 Size: 2185 4758 SbfI CC_TGCA'GG Cuts at: 4295 4295 Size: 6943 ScaI AGT'ACT Cuts at: 2934 5191 6903 2934 Size: 2257 1712 2974 Fragments arranged by size: 2974 2257 1712 SexAI A'CCwGG_T Cuts at: 2827 2997 2827 Size: 170 6773 SfcI C'TryA_G Cuts at: 826 1045 2785 3023 3320 4291 5426 6107 6298 Size: 219 1740 238 297 971 1135 681 191 Cuts at: 6298 826 Size: 1471 Fragments arranged by size: 1740 1471 1135 971 681 297 238 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6943 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6943 SmaI CCC'GGG Cuts at: 1872 2471 3789 4270 1872 Size: 599 1318 481 4545 Fragments arranged by size: 4545 1318 599 481 SmlI C'TyrA_G Cuts at: 820 1017 1943 3447 4154 5047 5948 6210 820 Size: 197 926 1504 707 893 901 262 1553 Fragments arranged by size: 1553 1504 926 901 893 707 262 197 SnaBI TAC'GTA Cuts at: 493 4734 493 Size: 4241 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6943 SphI G_CATG'C Cuts at: 1132 4301 1132 Size: 3169 3774 SspI AAT'ATT Cuts at: 5 52 4867 5 Size: 47 4815 2081 Fragments arranged by size: 4815 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6943 StyI C'CwwG_G Cuts at: 513 1065 2004 3519 3861 4382 513 Size: 552 939 1515 342 521 3074 Fragments arranged by size: 3074 1515 939 552 521 342 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2089 3495 4970 4987 5140 5325 5770 Size: 1255 509 1406 1475 17 153 185 445 Cuts at: 5770 325 Size: 1498 Fragments arranged by size: 1498 1475 1406 1255 509 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2880 2932 3207 Size: 274 80 33 51 1443 903 52 275 Cuts at: 3207 4188 5189 6901 96 Size: 981 1001 1712 138 Fragments arranged by size: 1712 1443 1001 981 903 275 274 138 80 52 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2910 2942 3408 3440 6903 6935 2910 Size: 32 466 32 3463 32 2918 Fragments arranged by size: 3463 2918 466 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2829 4181 1316 Size: 1513 1352 4078 Fragments arranged by size: 4078 1513 1352 XbaI T'CTAG_A Cuts at: 4279 4279 Size: 6943 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2188 2912 3985 2188 Size: 724 1073 5146 Fragments arranged by size: 5146 1073 724 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6943 XmaI C'CCGG_G Cuts at: 1870 2469 3787 4268 1870 Size: 599 1318 481 4545 Fragments arranged by size: 4545 1318 599 481 XmnI GAAnn'nnTTC Cuts at: 3687 4220 5072 3687 Size: 533 852 5558 Fragments arranged by size: 5558 852 533 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4751 276 Size: 53 83 186 936 3217 2468 Fragments arranged by size: 3217 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI Bsu36I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI