(Circular) (Six-base) MAPSORT of: pFN21AB4592.seq Check: 98 from: 1 to: 6508 pFN21AB4592 6508 bp With 182 enzymes: SgfI * February 27, 2009 14:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4318 278 Size: 53 83 186 936 2782 2468 Fragments arranged by size: 2782 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3098 3405 3851 1937 Size: 1161 307 446 4594 Fragments arranged by size: 4594 1161 446 307 Acc65I G'GTAC_C Cuts at: 3829 3829 Size: 6508 AclI AA'CG_TT Cuts at: 1563 4635 5008 1563 Size: 3072 373 3063 Fragments arranged by size: 3072 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2664 2949 4571 5955 6223 1632 Size: 1032 285 1622 1384 268 1917 Fragments arranged by size: 1917 1622 1384 1032 285 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6311 AflIII A'CryG_T Cuts at: 1203 3058 5407 6384 1203 Size: 1855 2349 977 1327 Fragments arranged by size: 2349 1855 1327 977 AgeI A'CCGG_T Cuts at: 5339 5339 Size: 6508 AhdI GACnn_n'nnGTC Cuts at: 5237 5237 Size: 6508 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6508 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6508 AlwNI CAG_nnn'CTG Cuts at: 2945 4251 5823 2945 Size: 1306 1572 3630 Fragments arranged by size: 3630 1572 1306 ApaI G_GGCC'C Cuts at: 3475 3475 Size: 6508 ApaLI G'TGCA_C Cuts at: 4565 5721 4565 Size: 1156 5352 ApoI r'AATT_y Cuts at: 1493 2263 2612 3015 3350 3370 3817 4043 4195 Size: 770 349 403 335 20 447 226 152 Cuts at: 4195 6304 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 770 447 403 349 335 226 152 20 AseI AT'TA_AT Cuts at: 160 1032 3555 5062 160 Size: 872 2523 1507 1606 Fragments arranged by size: 2523 1606 1507 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6508 AvaI C'yCGr_G Cuts at: 1870 1943 2024 3833 1870 Size: 73 81 1809 4545 Fragments arranged by size: 4545 1809 81 73 BamHI G'GATC_C Cuts at: 3838 4205 3838 Size: 367 6141 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3829 5284 618 Size: 325 217 627 2042 1455 1842 Fragments arranged by size: 2042 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2025 2100 2850 3475 3827 1391 Size: 634 75 750 625 352 4072 Fragments arranged by size: 4072 750 634 625 352 75 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2115 3759 928 Size: 971 216 1644 3677 Fragments arranged by size: 3677 1644 971 216 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4699 4733 1903 Size: 34 2762 34 3678 Fragments arranged by size: 3678 2762 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3052 4400 5610 3052 Size: 1348 1210 3950 Fragments arranged by size: 3950 1348 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6508 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5119 136 Size: 107 122 71 631 605 3447 1525 Fragments arranged by size: 3447 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2382 6503 2382 Size: 4121 2387 BlpI GC'TnA_GC Cuts at: 2250 2443 3925 2250 Size: 193 1482 4833 Fragments arranged by size: 4833 1482 193 Bme1580I G_kGCm'C Cuts at: 1790 3475 4569 5725 1790 Size: 1685 1094 1156 2573 Fragments arranged by size: 2573 1685 1156 1094 BmgBI CAC'GTC Cuts at: 1318 2113 2588 1318 Size: 795 475 5238 Fragments arranged by size: 5238 795 475 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2731 4239 5197 Size: 416 53 83 399 24 1314 1508 958 Cuts at: 5197 442 Size: 1753 Fragments arranged by size: 1753 1508 1314 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6508 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2834 5168 1239 Size: 1595 2334 2579 Fragments arranged by size: 2579 2334 1595 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2780 4633 5498 5796 2780 Size: 1853 865 298 3492 Fragments arranged by size: 3492 1853 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2990 3624 5171 882 Size: 617 1491 634 1547 2219 Fragments arranged by size: 2219 1547 1491 634 617 BsaAI yAC'GTr Cuts at: 493 1204 4299 493 Size: 711 3095 2702 Fragments arranged by size: 3095 2702 711 BsaBI GATnn'nnATC Cuts at: 3051 3227 6502 3051 Size: 176 3275 3057 Fragments arranged by size: 3275 3057 176 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4315 4697 5332 275 Size: 53 83 186 936 2782 382 635 1451 Fragments arranged by size: 2782 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4941 5339 5613 5760 6171 4941 Size: 398 274 147 411 5278 Fragments arranged by size: 5278 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3621 3651 1178 Size: 30 165 30 2218 30 4035 Fragments arranged by size: 4035 2218 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6508 BseYI C'CCAG_C Cuts at: 2257 3340 5711 6207 2257 Size: 1083 2371 496 2558 Fragments arranged by size: 2558 2371 1083 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2135 1163 Size: 573 399 5536 Fragments arranged by size: 5536 573 399 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2079 3958 4719 5747 1540 Size: 275 186 78 1879 761 1028 2301 Fragments arranged by size: 2301 1879 1028 761 275 186 78 BsiHKAI G_wGCw'C Cuts at: 2025 2100 2564 2850 4569 4654 5725 2025 Size: 75 464 286 1719 85 1071 2808 Fragments arranged by size: 2808 1719 1071 464 286 85 75 BsmI GAATG_Cn' Cuts at: 4019 4112 4019 Size: 93 6415 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2025 2100 2564 2850 3475 3827 4569 Size: 399 235 75 464 286 625 352 742 Cuts at: 4569 4654 5725 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 625 464 399 352 286 235 85 75 BspEI T'CCGG_A Cuts at: 6171 6171 Size: 6508 BspHI T'CATG_A Cuts at: 3438 4397 6127 3438 Size: 959 1730 3819 Fragments arranged by size: 3819 1730 959 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3863 844 Size: 3019 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4395 1394 Size: 429 2572 3507 Fragments arranged by size: 3507 2572 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2110 5003 5177 65 Size: 1183 862 2893 174 1396 Fragments arranged by size: 2893 1396 1183 862 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2030 5152 5339 1700 Size: 110 220 3122 187 2869 Fragments arranged by size: 3122 2869 220 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6508 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6508 BssSI C'ACGA_G Cuts at: 3392 4568 5580 3392 Size: 1176 1012 4320 Fragments arranged by size: 4320 1176 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2519 2945 3281 3604 794 Size: 1725 426 336 323 3698 Fragments arranged by size: 3698 1725 426 336 323 BstBI TT'CG_AA Cuts at: 5312 5312 Size: 6508 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6508 BstYI r'GATC_y Cuts at: 1972 2382 3838 4205 4590 4607 6059 6145 6157 Size: 410 1456 367 385 17 1452 86 12 Cuts at: 6157 6503 1972 Size: 346 1977 Fragments arranged by size: 1977 1456 1452 410 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3406 3406 Size: 6508 BtgI C'CryG_G Cuts at: 513 1065 2004 2859 513 Size: 552 939 855 4162 Fragments arranged by size: 4162 939 855 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5769 Fragments arranged by size: 5769 683 56 BtsI GCAGTG_nn' Cuts at: 4024 4818 4838 4024 Size: 794 20 5694 Fragments arranged by size: 5694 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6508 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6473 DraI TTT'AAA Cuts at: 3812 3812 Size: 6508 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3761 5515 809 Size: 507 2445 1754 1802 Fragments arranged by size: 2445 1802 1754 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3303 3660 3955 Size: 54 1425 50 275 18 1473 357 295 Cuts at: 3955 4844 6473 8 Size: 889 1629 43 Fragments arranged by size: 1629 1473 1425 889 357 295 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3955 1537 Size: 275 2143 4090 Fragments arranged by size: 4090 2143 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2334 4438 1353 Size: 981 2104 3423 Fragments arranged by size: 3423 2104 981 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3287 5095 5469 5615 387 Size: 1064 138 1698 1808 374 146 1280 Fragments arranged by size: 1808 1698 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2023 2098 2848 2023 Size: 75 750 5683 Fragments arranged by size: 5683 750 75 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2664 2834 2949 4571 5168 5955 6223 Size: 393 1032 170 115 1622 597 787 268 Cuts at: 6223 1239 Size: 1524 Fragments arranged by size: 1622 1524 1032 787 597 393 268 170 115 EcoO109I rG'GnC_Cy Cuts at: 2178 2285 2864 2178 Size: 107 579 5822 Fragments arranged by size: 5822 579 107 EcoRI G'AATT_C Cuts at: 3350 3370 3817 4195 3350 Size: 20 447 378 5663 Fragments arranged by size: 5663 447 378 20 FspI TGC'GCA Cuts at: 5014 5014 Size: 6508 HaeII r_GCGC'y Cuts at: 1678 5655 1678 Size: 3977 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5149 5181 5223 5255 1373 Size: 32 135 32 3577 32 42 32 2626 Fragments arranged by size: 3577 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3852 4098 669 Size: 988 281 1914 246 3079 Fragments arranged by size: 3079 1914 988 281 246 HindIII A'AGCT_T Cuts at: 748 2415 2718 748 Size: 1667 303 4538 Fragments arranged by size: 4538 1667 303 HpaI GTT'AAC Cuts at: 4098 4098 Size: 6508 KpnI G_GTAC'C Cuts at: 3833 3833 Size: 6508 MfeI C'AATT_G Cuts at: 2151 4107 2151 Size: 1956 4552 MluI A'CGCG_T Cuts at: 6384 6384 Size: 6508 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2146 5622 5806 1305 Size: 841 3476 184 2007 Fragments arranged by size: 3476 2007 841 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3305 3662 10 Size: 54 1425 343 1473 357 2856 Fragments arranged by size: 2856 1473 1425 357 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2479 2576 3005 4466 4825 4984 518 Size: 648 1313 97 429 1461 359 159 2042 Fragments arranged by size: 2042 1461 1313 648 429 359 159 97 MspA1I CmG'CkG Cuts at: 1606 2045 2257 2942 3325 3340 3924 4601 5749 Size: 439 212 685 383 15 584 677 1148 Cuts at: 5749 5994 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 685 677 584 439 383 245 212 15 NcoI C'CATG_G Cuts at: 513 1065 2004 2859 513 Size: 552 939 855 4162 Fragments arranged by size: 4162 939 855 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6508 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6508 NotI GC'GGCC_GC Cuts at: 3955 3955 Size: 6508 NspI r_CATG'y Cuts at: 1132 1229 2484 3062 3866 5411 1132 Size: 97 1255 578 804 1545 2229 Fragments arranged by size: 2229 1545 1255 804 578 97 PasI CC'CwG_GG Cuts at: 1348 2508 1348 Size: 1160 5348 PciI A'CATG_T Cuts at: 3058 5407 3058 Size: 2349 4159 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6508 PmeI GTTT'AAAC Cuts at: 3812 3812 Size: 6508 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4577 4609 6090 6122 4577 Size: 32 1481 32 4963 Fragments arranged by size: 4963 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2178 2285 2864 2178 Size: 107 579 5822 Fragments arranged by size: 5822 579 107 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6508 PsiI TTA'TAA Cuts at: 4078 4078 Size: 6508 PspOMI G'GGCC_C Cuts at: 3471 3471 Size: 6508 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3120 3152 3630 3662 3120 Size: 32 478 32 5966 Fragments arranged by size: 5966 478 32 32 PstI C_TGCA'G Cuts at: 830 3860 830 Size: 3030 3478 PvuI CG_AT'CG Cuts at: 2001 2079 2001 Size: 78 6430 PvuII CAG'CTG Cuts at: 2257 2942 3340 2257 Size: 685 398 5425 Fragments arranged by size: 5425 685 398 SacI G_AGCT'C Cuts at: 2025 2100 2850 2025 Size: 75 750 5683 Fragments arranged by size: 5683 750 75 SalI G'TCGA_C Cuts at: 1936 3850 1936 Size: 1914 4594 SbfI CC_TGCA'GG Cuts at: 3860 3860 Size: 6508 ScaI AGT'ACT Cuts at: 3729 4756 6468 3729 Size: 1027 1712 3769 Fragments arranged by size: 3769 1712 1027 SfcI C'TryA_G Cuts at: 826 1045 3731 3856 4991 5672 5863 826 Size: 219 2686 125 1135 681 191 1471 Fragments arranged by size: 2686 1471 1135 681 219 191 125 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6508 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6508 SmaI CCC'GGG Cuts at: 1872 3835 1872 Size: 1963 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2759 4612 5513 5775 820 Size: 197 926 816 1853 901 262 1553 Fragments arranged by size: 1853 1553 926 901 816 262 197 SnaBI TAC'GTA Cuts at: 493 4299 493 Size: 3806 2702 SpeI A'CTAG_T Cuts at: 152 2669 3445 152 Size: 2517 776 3215 Fragments arranged by size: 3215 2517 776 SphI G_CATG'C Cuts at: 1132 3866 1132 Size: 2734 3774 SspI AAT'ATT Cuts at: 5 52 4432 5 Size: 47 4380 2081 Fragments arranged by size: 4380 2081 47 StuI AGG'CCT Cuts at: 1517 3572 1517 Size: 2055 4453 StyI C'CwwG_G Cuts at: 513 1065 2004 2859 3947 513 Size: 552 939 855 1088 3074 Fragments arranged by size: 3074 1088 939 855 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2597 3332 4535 4552 4705 4890 5335 Size: 1255 1017 735 1203 17 153 185 445 Cuts at: 5335 325 Size: 1498 Fragments arranged by size: 1498 1255 1203 1017 735 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3727 4754 6466 Size: 274 80 33 51 1443 1750 1027 1712 Cuts at: 6466 96 Size: 138 Fragments arranged by size: 1750 1712 1443 1027 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2170 2202 6468 6500 2170 Size: 32 4266 32 2178 Fragments arranged by size: 4266 2178 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6508 XbaI T'CTAG_A Cuts at: 3691 3844 3691 Size: 153 6355 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3268 3268 Size: 6508 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6508 XmaI C'CCGG_G Cuts at: 1870 3833 1870 Size: 1963 4545 XmnI GAAnn'nnTTC Cuts at: 2655 3132 4637 2655 Size: 477 1505 4526 Fragments arranged by size: 4526 1505 477 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4316 276 Size: 53 83 186 936 2782 2468 Fragments arranged by size: 2782 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI