Restriction map for
pFN21AB4399
(Linear) (Six-base) MAPSORT of: KIBB4399.seq Check: 1292 from: 1 to: 6984
KIBB4399.orf
With 182 enzymes: SgfI *
July 18, 2007 13:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 4691 5546 5946 6984
Size: 4691 855 400 1038
Fragments arranged by size:
4691 1038 855 400
Acc65I G'GTAC_C
Cuts at: 0 1796 6984
Size: 1796 5188
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 694 1014 1795 2203 2284 3560 4282 4888
Size: 694 320 781 408 81 1276 722 606
Cuts at: 4888 4975 5044 5709 5859 6984
Size: 87 69 665 150 1125
Fragments arranged by size:
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320 150 87 81 69
AfeI AGC'GCT
Cuts at: 0 6190 6984
Size: 6190 794
AflIII A'CryG_T
Cuts at: 0 5063 6984
Size: 5063 1921
AleI CACnn'nnGTG
Cuts at: 0 805 6984
Size: 805 6179
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3323 4178 4543 4907 5389 6984
Size: 3323 855 365 364 482 1595
Fragments arranged by size:
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AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 385 417 6984
Size: 385 32 6567
Fragments arranged by size:
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AlwNI CAG_nnn'CTG
Cuts at: 0 1020 1184 1461 1545 2475 2529 2823 3060
Size: 1020 164 277 84 930 54 294 237
Cuts at: 3060 3135 3207 3286 3297 3426 3613 4566 4629
Size: 75 72 79 11 129 187 953 63
Cuts at: 4629 4737 4947 5376 5661 5882 6375 6605 6897
Size: 108 210 429 285 221 493 230 292
Cuts at: 6897 6984
Size: 87
Fragments arranged by size:
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277 237 230 221 210 187 164 129
108 87 84 79 75 72 63 54
11
ApaI G_GGCC'C
Cuts at: 0 2340 4941 6581 6984
Size: 2340 2601 1640 403
Fragments arranged by size:
2601 2340 1640 403
ApaLI G'TGCA_C
Cuts at: 0 2866 5509 6984
Size: 2866 2643 1475
Fragments arranged by size:
2866 2643 1475
ApoI r'AATT_y
Cuts at: 0 5713 6984
Size: 5713 1271
AvaI C'yCGr_G
Cuts at: 0 206 1570 4218 5207 5461 6042 6342 6984
Size: 206 1364 2648 989 254 581 300 642
Fragments arranged by size:
2648 1364 989 642 581 300 254 206
AvrII C'CTAG_G
Cuts at: 0 4398 6984
Size: 4398 2586
BamHI G'GATC_C
Cuts at: 0 3617 6984
Size: 3617 3367
BanI G'GyrC_C
Cuts at: 0 1559 1796 2129 3335 4323 4679 4852 5416
Size: 1559 237 333 1206 988 356 173 564
Cuts at: 5416 5681 6984
Size: 265 1303
Fragments arranged by size:
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237 173
BanII G_rGCy'C
Cuts at: 0 403 1241 1440 2057 2340 2486 3587 3857
Size: 403 838 199 617 283 146 1101 270
Cuts at: 3857 4141 4693 4814 4941 5354 5534 6089 6452
Size: 284 552 121 127 413 180 555 363
Cuts at: 6452 6497 6581 6984
Size: 45 84 403
Fragments arranged by size:
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363 284 283 270 199 180 146 127
121 84 45
BbeI G_GCGC'C
Cuts at: 0 1563 4856 5685 6984
Size: 1563 3293 829 1299
Fragments arranged by size:
3293 1563 1299 829
BbsI GAAGACnn'nnnn_
Cuts at: 0 562 1697 2698 4117 6984
Size: 562 1135 1001 1419 2867
Fragments arranged by size:
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BbvCI CC'TCA_GC
Cuts at: 0 3550 3707 4787 6062 6772 6984
Size: 3550 157 1080 1275 710 212
Fragments arranged by size:
3550 1275 1080 710 212 157
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 187 197 221 231 849 883 1396 1430
Size: 187 10 24 10 618 34 513 34
Cuts at: 1430 5656 5690 6984
Size: 4226 34 1294
Fragments arranged by size:
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24 10 10
BciVI GTATCCnnnnn_n'
Cuts at: 0 3432 6984
Size: 3432 3552
BglI GCCn_nnn'nGGC
Cuts at: 0 734 2335 3022 3535 3793 4753 4936 5488
Size: 734 1601 687 513 258 960 183 552
Cuts at: 5488 6058 6181 6984
Size: 570 123 803
Fragments arranged by size:
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258 183 123
BglII A'GATC_T
Cuts at: 0 1973 2084 6984
Size: 1973 111 4900
Fragments arranged by size:
4900 1973 111
BlpI GC'TnA_GC
Cuts at: 0 105 519 722 1864 3287 4256 6984
Size: 105 414 203 1142 1423 969 2728
Fragments arranged by size:
2728 1423 1142 969 414 203 105
Bme1580I G_kGCm'C
Cuts at: 0 2132 2340 2870 3338 4682 4941 5513 6581
Size: 2132 208 530 468 1344 259 572 1068
Cuts at: 6581 6984
Size: 403
Fragments arranged by size:
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208
BmgBI CAC'GTC
Cuts at: 0 3009 5031 5469 5571 6762 6984
Size: 3009 2022 438 102 1191 222
Fragments arranged by size:
3009 2022 1191 438 222 102
BmrI ACTGGGnnnn_n'
Cuts at: 0 4026 4497 6275 6434 6984
Size: 4026 471 1778 159 550
Fragments arranged by size:
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BmtI G_CTAG'C
Cuts at: 0 931 6984
Size: 931 6053
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 300 4511 6984
Size: 300 4211 2473
Fragments arranged by size:
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BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 308 343 358 409 582 721 1216
Size: 295 13 35 15 51 173 139 495
Cuts at: 1216 1462 1483 1918 2098 2200 2386 2395 2425
Size: 246 21 435 180 102 186 9 30
Cuts at: 2425 2659 2668 3019 3061 3448 3823 3841 5077
Size: 234 9 351 42 387 375 18 1236
Cuts at: 5077 5125 5368 5908 6046 6223 6290 6592 6984
Size: 48 243 540 138 177 67 302 392
Fragments arranged by size:
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302 295 246 243 234 186 180 177
173 139 138 102 67 51 48 42
35 30 21 18 15 13 9 9
Bpu10I CC'TnA_GC
Cuts at: 0 1454 3077 3550 3707 4787 6062 6628 6772
Size: 1454 1623 473 157 1080 1275 566 144
Cuts at: 6772 6984
Size: 212
Fragments arranged by size:
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144
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 352 3693 4380 4684 5068 6241 6257 6984
Size: 352 3341 687 304 384 1173 16 727
Fragments arranged by size:
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BsaI GGTCTCn'nnnn_
Cuts at: 0 1297 1396 1567 2887 5086 5206 5773 6243
Size: 1297 99 171 1320 2199 120 567 470
Cuts at: 6243 6984
Size: 741
Fragments arranged by size:
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99
BsaAI yAC'GTr
Cuts at: 0 5172 6984
Size: 5172 1812
BsaBI GATnn'nnATC
Cuts at: 0 325 6984
Size: 325 6659
BsaHI Gr'CG_yC
Cuts at: 0 71 1007 1560 2282 2573 4745 4853 5480
Size: 71 936 553 722 291 2172 108 627
Cuts at: 5480 5682 6926 6984
Size: 202 1244 58
Fragments arranged by size:
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108 71 58
BsaWI w'CCGG_w
Cuts at: 0 1283 3620 6984
Size: 1283 2337 3364
Fragments arranged by size:
3364 2337 1283
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 385 415 580 610 814 844 3034 3064
Size: 385 30 165 30 204 30 2190 30
Cuts at: 3064 3109 3139 4807 4837 5050 5080 5731 5761
Size: 45 30 1668 30 213 30 651 30
Cuts at: 5761 6814 6844 6984
Size: 1053 30 140
Fragments arranged by size:
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140 45 30 30 30 30 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 739 1438 1873 2383 2392 2905 3133 4312
Size: 739 699 435 510 9 513 228 1179
Cuts at: 4312 4354 4361 4498 4681 5734 5796 5947 6274
Size: 42 7 137 183 1053 62 151 327
Cuts at: 6274 6292 6832 6835 6984
Size: 18 540 3 149
Fragments arranged by size:
1179 1053 739 699 540 513 510 435
327 228 183 151 149 137 62 42
18 9 7 3
BseYI C'CCAG_C
Cuts at: 0 65 732 1879 3053 3168 3613 4115 4302
Size: 65 667 1147 1174 115 445 502 187
Cuts at: 4302 5541 5599 5690 6002 6179 6984
Size: 1239 58 91 312 177 805
Fragments arranged by size:
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187 177 115 91 65 58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1597 2677 3766 4054 5380 5575 6556 6984
Size: 1597 1080 1089 288 1326 195 981 428
Fragments arranged by size:
1597 1326 1089 1080 981 428 288 195
BsiEI CG_ry'CG
Cuts at: 0 4721 4911 5492 6984
Size: 4721 190 581 1492
Fragments arranged by size:
4721 1492 581 190
BsiHKAI G_wGCw'C
Cuts at: 0 284 707 1241 2018 2057 2402 2486 2870
Size: 284 423 534 777 39 345 84 384
Cuts at: 2870 3857 4141 4693 4814 5354 5513 5534 6629
Size: 987 284 552 121 540 159 21 1095
Cuts at: 6629 6853 6984
Size: 224 131
Fragments arranged by size:
1095 987 777 552 540 534 423 384
345 284 284 224 159 131 121 84
39 21
BsmI GAATG_Cn'
Cuts at: 0 709 3456 5256 6984
Size: 709 2747 1800 1728
Fragments arranged by size:
2747 1800 1728 709
BsmBI CGTCTCn'nnnn_
Cuts at: 0 4462 6984
Size: 4462 2522
Bsp1286I G_dGCh'C
Cuts at: 0 284 403 707 1241 1440 2018 2057 2132
Size: 284 119 304 534 199 578 39 75
Cuts at: 2132 2340 2402 2486 2870 3338 3587 3857 4141
Size: 208 62 84 384 468 249 270 284
Cuts at: 4141 4682 4693 4814 4941 5354 5513 5534 6089
Size: 541 11 121 127 413 159 21 555
Cuts at: 6089 6452 6497 6581 6629 6853 6984
Size: 363 45 84 48 224 131
Fragments arranged by size:
578 555 541 534 468 413 384 363
304 284 284 270 249 224 208 199
159 131 127 121 119 84 84 75
62 48 45 39 21 11
BspEI T'CCGG_A
Cuts at: 0 3620 6984
Size: 3620 3364
BspHI T'CATG_A
Cuts at: 0 4547 5822 6984
Size: 4547 1275 1162
Fragments arranged by size:
4547 1275 1162
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 251 947 1544 1703 2522 2953 3301 4154
Size: 251 696 597 159 819 431 348 853
Cuts at: 4154 4691 5402 5546 5946 6383 6984
Size: 537 711 144 400 437 601
Fragments arranged by size:
853 819 711 696 601 597 537 437
431 400 348 251 159 144
BsrBI CCG'CTC
Cuts at: 0 153 747 1428 2835 5235 5526 6009 6984
Size: 153 594 681 1407 2400 291 483 975
Fragments arranged by size:
2400 1407 975 681 594 483 291 153
BsrDI GCAATG_nn'
Cuts at: 0 1301 6984
Size: 1301 5683
BsrFI r'CCGG_y
Cuts at: 0 644 2288 4073 6507 6984
Size: 644 1644 1785 2434 477
Fragments arranged by size:
2434 1785 1644 644 477
BsrGI T'GTAC_A
Cuts at: 0 381 1139 2549 6984
Size: 381 758 1410 4435
Fragments arranged by size:
4435 1410 758 381
BssHII G'CGCG_C
Cuts at: 0 2113 6984
Size: 2113 4871
BssSI C'ACGA_G
Cuts at: 0 1330 6984
Size: 1330 5654
BstAPI GCAn_nnn'nTGC
Cuts at: 0 332 1020 1545 2475 2529 3135 3147 3297
Size: 332 688 525 930 54 606 12 150
Cuts at: 3297 4536 5661 6534 6984
Size: 1239 1125 873 450
Fragments arranged by size:
1239 1125 930 873 688 606 525 450
332 150 54 12
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2739 6669 6984
Size: 2739 3930 315
Fragments arranged by size:
3930 2739 315
BstYI r'GATC_y
Cuts at: 0 1973 2084 3204 3617 4832 5423 5936 6984
Size: 1973 111 1120 413 1215 591 513 1048
Fragments arranged by size:
1973 1215 1120 1048 591 513 413 111
Bsu36I CC'TnA_GG
Cuts at: 0 4967 6984
Size: 4967 2017
BtgI C'CryG_G
Cuts at: 0 1046 2058 2385 4190 4594 6170 6443 6514
Size: 1046 1012 327 1805 404 1576 273 71
Cuts at: 6514 6522 6690 6804 6984
Size: 8 168 114 180
Fragments arranged by size:
1805 1576 1046 1012 404 327 273 180
168 114 71 8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1713 2264 2955 5286 6984
Size: 1713 551 691 2331 1698
Fragments arranged by size:
2331 1713 1698 691 551
BtsI GCAGTG_nn'
Cuts at: 0 330 857 1585 1855 4564 5116 6984
Size: 330 527 728 270 2709 552 1868
Fragments arranged by size:
2709 1868 728 552 527 330 270
ClaI AT'CG_AT
Cuts at: 0 3995 6984
Size: 3995 2989
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 35 2909 2944 6201 6236 6984
Size: 35 2874 35 3257 35 748
Fragments arranged by size:
3257 2874 748 35 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 2804 5508 6120 6984
Size: 2804 2704 612 864
Fragments arranged by size:
2804 2704 864 612
DrdI GACnn_nn'nnGTC
Cuts at: 0 487 1816 2650 2826 6984
Size: 487 1329 834 176 4158
Fragments arranged by size:
4158 1329 834 487 176
EaeI y'GGCC_r
Cuts at: 0 8 2612 2751 3011 4718 4908 4928 5294
Size: 8 2604 139 260 1707 190 20 366
Cuts at: 5294 5378 5489 5573 6095 6173 6323 6984
Size: 84 111 84 522 78 150 661
Fragments arranged by size:
2604 1707 661 522 366 260 190 150
139 111 84 84 78 20 8
EagI C'GGCC_G
Cuts at: 0 4718 4908 5489 6984
Size: 4718 190 581 1495
Fragments arranged by size:
4718 1495 581 190
EarI CTCTTCn'nnn_
Cuts at: 0 1603 2426 4841 5033 5145 5183 6347 6805
Size: 1603 823 2415 192 112 38 1164 458
Cuts at: 6805 6949 6984
Size: 144 35
Fragments arranged by size:
2415 1603 1164 823 458 192 144 112
38 35
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1899 2524 4657 5050 5722 6984
Size: 1899 625 2133 393 672 1262
Fragments arranged by size:
2133 1899 1262 672 625 393
EcoICRI GAG'CTC
Cuts at: 0 1239 2055 2484 3855 4139 4691 4812 5352
Size: 1239 816 429 1371 284 552 121 540
Cuts at: 5352 5532 6984
Size: 180 1452
Fragments arranged by size:
1452 1371 1239 816 552 540 429 284
180 121
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 308 343 358 409 582 694 721
Size: 295 13 35 15 51 173 112 27
Cuts at: 721 1014 1216 1462 1483 1795 1918 2098 2200
Size: 293 202 246 21 312 123 180 102
Cuts at: 2200 2203 2284 2386 2395 2425 2659 2668 3019
Size: 3 81 102 9 30 234 9 351
Cuts at: 3019 3061 3448 3560 3823 3841 4282 4888 4975
Size: 42 387 112 263 18 441 606 87
Cuts at: 4975 5044 5077 5125 5368 5709 5859 5908 6046
Size: 69 33 48 243 341 150 49 138
Cuts at: 6046 6223 6290 6592 6984
Size: 177 67 302 392
Fragments arranged by size:
606 441 392 387 351 341 312 302
295 293 263 246 243 234 202 180
177 173 150 138 123 112 112 102
102 87 81 69 67 51 49 48
42 35 33 30 27 21 18 15
13 9 9 3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1453 2680 3280 4698 6457 6984
Size: 1453 1227 600 1418 1759 527
Fragments arranged by size:
1759 1453 1418 1227 600 527
EcoO109I rG'GnC_Cy
Cuts at: 0 125 240 1181 1446 2187 2337 2472 2643
Size: 125 115 941 265 741 150 135 171
Cuts at: 2643 2955 3132 3432 4227 4659 4944 6462 6577
Size: 312 177 300 795 432 285 1518 115
Cuts at: 6577 6602 6984
Size: 25 382
Fragments arranged by size:
1518 941 795 741 432 382 312 300
285 265 177 171 150 135 125 115
115 25
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 630 1111 6780 6984
Size: 630 481 5669 204
Fragments arranged by size:
5669 630 481 204
FspI TGC'GCA
Cuts at: 0 325 6984
Size: 325 6659
FspAI rTGC'GCAy
Cuts at: 0 325 6984
Size: 325 6659
HaeII r_GCGC'y
Cuts at: 0 729 1278 1329 1563 4190 4674 4856 5662
Size: 729 549 51 234 2627 484 182 806
Cuts at: 5662 5685 6192 6984
Size: 23 507 792
Fragments arranged by size:
2627 806 792 729 549 507 484 234
182 51 23
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 88 120 775 807 1629 1661 3034 3066
Size: 88 32 655 32 822 32 1373 32
Cuts at: 3066 5731 5763 5791 5823 6255 6287 6322 6354
Size: 2665 32 28 32 432 32 35 32
Cuts at: 6354 6707 6739 6814 6846 6984
Size: 353 32 75 32 138
Fragments arranged by size:
2665 1373 822 655 432 353 138 88
75 35 32 32 32 32 32 32
32 32 32 32 28
KasI G'GCGC_C
Cuts at: 0 1559 4852 5681 6984
Size: 1559 3293 829 1303
Fragments arranged by size:
3293 1559 1303 829
KpnI G_GTAC'C
Cuts at: 0 1800 6984
Size: 1800 5184
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1661 2810 4166 6620 6984
Size: 1661 1149 1356 2454 364
Fragments arranged by size:
2454 1661 1356 1149 364
MscI TGG'CCA
Cuts at: 0 2753 4930 5575 6097 6984
Size: 2753 2177 645 522 887
Fragments arranged by size:
2753 2177 887 645 522
MslI CAynn'nnrTG
Cuts at: 0 578 805 4861 5365 6407 6984
Size: 578 227 4056 504 1042 577
Fragments arranged by size:
4056 1042 578 577 504 227
MspA1I CmG'CkG
Cuts at: 0 65 602 732 923 1020 1366 1879 2181
Size: 65 537 130 191 97 346 513 302
Cuts at: 2181 3060 3102 3186 3423 3750 4014 4203 4419
Size: 879 42 84 237 327 264 189 216
Cuts at: 4419 4596 4629 4717 4926 5373 5913 6002 6101
Size: 177 33 88 209 447 540 89 99
Cuts at: 6101 6179 6516 6984
Size: 78 337 468
Fragments arranged by size:
879 540 537 513 468 447 346 337
327 302 264 237 216 209 191 189
177 130 99 97 89 88 84 78
65 42 33
NaeI GCC'GGC
Cuts at: 0 6509 6984
Size: 6509 475
NarI GG'CG_CC
Cuts at: 0 1560 4853 5682 6984
Size: 1560 3293 829 1302
Fragments arranged by size:
3293 1560 1302 829
NcoI C'CATG_G
Cuts at: 0 4190 6522 6690 6804 6984
Size: 4190 2332 168 114 180
Fragments arranged by size:
4190 2332 180 168 114
NdeI CA'TA_TG
Cuts at: 0 5595 6984
Size: 5595 1389
NgoMIV G'CCGG_C
Cuts at: 0 6507 6984
Size: 6507 477
NheI G'CTAG_C
Cuts at: 0 927 6984
Size: 927 6057
NspI r_CATG'y
Cuts at: 0 261 1611 3267 3310 5067 5370 6021 6721
Size: 261 1350 1656 43 1757 303 651 700
Cuts at: 6721 6984
Size: 263
Fragments arranged by size:
1757 1656 1350 700 651 303 263 261
43
PasI CC'CwG_GG
Cuts at: 0 2340 2418 3456 4941 5795 6675 6984
Size: 2340 78 1038 1485 854 880 309
Fragments arranged by size:
2340 1485 1038 880 854 309 78
PciI A'CATG_T
Cuts at: 0 5063 6984
Size: 5063 1921
PflMI CCAn_nnn'nTGG
Cuts at: 0 336 432 2496 2903 3426 4059 5541 5571
Size: 336 96 2064 407 523 633 1482 30
Cuts at: 5571 5697 6984
Size: 126 1287
Fragments arranged by size:
2064 1482 1287 633 523 407 336 126
96 30
PfoI T'CCnGG_A
Cuts at: 0 127 1111 1601 1823 2090 2216 2363 2675
Size: 127 984 490 222 267 126 147 312
Cuts at: 2675 3509 4379 4388 5195 5345 5564 5749 5965
Size: 834 870 9 807 150 219 185 216
Cuts at: 5965 6604 6984
Size: 639 380
Fragments arranged by size:
984 870 834 807 639 490 380 312
267 222 219 216 185 150 147 127
126 9
PmlI CAC'GTG
Cuts at: 0 5172 6984
Size: 5172 1812
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 385 417 4360 4392 6351 6383 6984
Size: 385 32 3943 32 1959 32 601
Fragments arranged by size:
3943 1959 601 385 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 125 240 1446 2187 2643 2955 4227 4659
Size: 125 115 1206 741 456 312 1272 432
Cuts at: 4659 6462 6602 6984
Size: 1803 140 382
Fragments arranged by size:
1803 1272 1206 741 456 432 382 312
140 125 115
PshAI GACnn'nnGTC
Cuts at: 0 1006 2516 4237 6984
Size: 1006 1510 1721 2747
Fragments arranged by size:
2747 1721 1510 1006
PspOMI G'GGCC_C
Cuts at: 0 2336 4937 6577 6984
Size: 2336 2601 1640 407
Fragments arranged by size:
2601 2336 1640 407
PspXI vC'TCGA_Gb
Cuts at: 0 206 6984
Size: 206 6778
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2542 2574 5059 5091 6601 6633 6984
Size: 2542 32 2485 32 1510 32 351
Fragments arranged by size:
2542 2485 1510 351 32 32 32
PstI C_TGCA'G
Cuts at: 0 1025 1541 1553 1592 2471 2534 3140 3152
Size: 1025 516 12 39 879 63 606 12
Cuts at: 3152 3191 3203 3302 3609 4094 4445 4571 4616
Size: 39 12 99 307 485 351 126 45
Cuts at: 4616 4688 4733 4959 5588 5912 5954 6380 6842
Size: 72 45 226 629 324 42 426 462
Cuts at: 6842 6984
Size: 142
Fragments arranged by size:
1025 879 629 606 516 485 462 426
351 324 307 226 142 126 99 72
63 45 45 42 39 39 12 12
12
PvuII CAG'CTG
Cuts at: 0 602 732 1020 1366 1879 2181 3060 3186
Size: 602 130 288 346 513 302 879 126
Cuts at: 3186 3750 4014 4203 4419 4629 4926 5373 6179
Size: 564 264 189 216 210 297 447 806
Cuts at: 6179 6984
Size: 805
Fragments arranged by size:
879 806 805 602 564 513 447 346
302 297 288 264 216 210 189 130
126
SacI G_AGCT'C
Cuts at: 0 1241 2057 2486 3857 4141 4693 4814 5354
Size: 1241 816 429 1371 284 552 121 540
Cuts at: 5354 5534 6984
Size: 180 1450
Fragments arranged by size:
1450 1371 1241 816 552 540 429 284
180 121
SacII CC_GC'GG
Cuts at: 0 4597 6517 6984
Size: 4597 1920 467
Fragments arranged by size:
4597 1920 467
SapI GCTCTTCn'nnn_
Cuts at: 0 1603 5033 6805 6984
Size: 1603 3430 1772 179
Fragments arranged by size:
3430 1772 1603 179
SbfI CC_TGCA'GG
Cuts at: 0 1541 3152 3203 6984
Size: 1541 1611 51 3781
Fragments arranged by size:
3781 1611 1541 51
ScaI AGT'ACT
Cuts at: 0 4765 6984
Size: 4765 2219
SexAI A'CCwGG_T
Cuts at: 0 2800 6984
Size: 2800 4184
SfcI C'TryA_G
Cuts at: 0 1021 1537 1549 1588 1699 2467 2530 3136
Size: 1021 516 12 39 111 768 63 606
Cuts at: 3136 3148 3187 3199 3298 3605 3826 4090 4441
Size: 12 39 12 99 307 221 264 351
Cuts at: 4441 4567 4612 4684 4729 4955 5584 5908 5950
Size: 126 45 72 45 226 629 324 42
Cuts at: 5950 6376 6838 6984
Size: 426 462 146
Fragments arranged by size:
1021 768 629 606 516 462 426 351
324 307 264 226 221 146 126 111
99 72 63 45 45 42 39 39
12 12 12
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2335 3793 4936 6984
Size: 2335 1458 1143 2048
Fragments arranged by size:
2335 2048 1458 1143
SfoI GGC'GCC
Cuts at: 0 1561 4854 5683 6984
Size: 1561 3293 829 1301
Fragments arranged by size:
3293 1561 1301 829
SmaI CCC'GGG
Cuts at: 0 4220 5209 6344 6984
Size: 4220 989 1135 640
Fragments arranged by size:
4220 1135 989 640
SmlI C'TyrA_G
Cuts at: 0 206 367 1570 3672 4359 4663 5083 6042
Size: 206 161 1203 2102 687 304 420 959
Cuts at: 6042 6220 6272 6984
Size: 178 52 712
Fragments arranged by size:
2102 1203 959 712 687 420 304 206
178 161 52
SpeI A'CTAG_T
Cuts at: 0 1161 6984
Size: 1161 5823
SphI G_CATG'C
Cuts at: 0 261 3310 5370 6984
Size: 261 3049 2060 1614
Fragments arranged by size:
3049 2060 1614 261
StuI AGG'CCT
Cuts at: 0 139 1027 2041 3193 3223 3796 4792 5926
Size: 139 888 1014 1152 30 573 996 1134
Cuts at: 5926 6984
Size: 1058
Fragments arranged by size:
1152 1134 1058 1014 996 888 573 139
30
StyI C'CwwG_G
Cuts at: 0 140 311 524 1737 3384 3501 4190 4398
Size: 140 171 213 1213 1647 117 689 208
Cuts at: 4398 4838 6522 6690 6804 6984
Size: 440 1684 168 114 180
Fragments arranged by size:
1684 1647 1213 689 440 213 208 180
171 168 140 117 114
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 177 1420 2771 3253 4467 5110 5533 6984
Size: 177 1243 1351 482 1214 643 423 1451
Fragments arranged by size:
1451 1351 1243 1214 643 482 423 177
TatI w'GTAC_w
Cuts at: 0 381 917 1139 2549 4763 6617 6984
Size: 381 536 222 1410 2214 1854 367
Fragments arranged by size:
2214 1854 1410 536 381 367 222
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 814 846 3109 3141 6104 6136 6984
Size: 814 32 2263 32 2963 32 848
Fragments arranged by size:
2963 2263 848 814 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1345 2098 2337 2943 6984
Size: 1345 753 239 606 4041
Fragments arranged by size:
4041 1345 753 606 239
XhoI C'TCGA_G
Cuts at: 0 206 1570 6042 6984
Size: 206 1364 4472 942
Fragments arranged by size:
4472 1364 942 206
XmaI C'CCGG_G
Cuts at: 0 4218 5207 6342 6984
Size: 4218 989 1135 642
Fragments arranged by size:
4218 1135 989 642
XmnI GAAnn'nnTTC
Cuts at: 0 161 1597 1924 2233 2435 4375 5254 6958
Size: 161 1436 327 309 202 1940 879 1704
Cuts at: 6958 6984
Size: 26
Fragments arranged by size:
1940 1704 1436 879 327 309 202 161
26
Enzymes that do cut:
AarI Acc65I AcuI AfeI AflIII AleI AlfI AloI
AlwNI ApaI ApaLI ApoI AvaI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I FalI FspI FspAI HaeII Hin4I
KasI KpnI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NspI PasI PciI PflMI
PfoI PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI
PstI PvuII SacI SacII SapI SbfI ScaI SexAI
SfcI SfiI SfoI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AccI AclI AflII AgeI AhdI AscI AseI
AsiSI BaeI BclI BfrBI BsiWI BstBI BstEII BstZ17I
DraI EcoRI EcoRV FseI HincII HindIII HpaI MfeI
MluI NotI NruI NsiI PacI PmeI PsiI PvuI
RsrII SalI SanDI SgfI SgrAI SnaBI SrfI SspI
SwaI Tth111I XbaI ZraI