(Circular) (Six-base) MAPSORT of: pFN21AB4360.seq Check: 5169 from: 1 to: 7090 pFN21AB4360 7090 bp With 182 enzymes: SgfI * February 27, 2009 14:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3501 3501 Size: 7090 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3333 4900 278 Size: 53 83 186 936 1797 1567 2468 Fragments arranged by size: 2468 1797 1567 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3050 4065 4433 1937 Size: 1113 1015 368 4594 Fragments arranged by size: 4594 1113 1015 368 Acc65I G'GTAC_C Cuts at: 4411 4411 Size: 7090 AclI AA'CG_TT Cuts at: 1563 5217 5590 1563 Size: 3654 373 3063 Fragments arranged by size: 3654 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4322 5153 6537 6805 1632 Size: 2690 831 1384 268 1917 Fragments arranged by size: 2690 1917 1384 831 268 AfeI AGC'GCT Cuts at: 2601 2601 Size: 7090 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6893 AflIII A'CryG_T Cuts at: 1203 5989 6966 1203 Size: 4786 977 1327 Fragments arranged by size: 4786 1327 977 AgeI A'CCGG_T Cuts at: 5921 5921 Size: 7090 AhdI GACnn_n'nnGTC Cuts at: 5819 5819 Size: 7090 AleI CACnn'nnGTG Cuts at: 1166 3209 1166 Size: 2043 5047 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7090 AlwNI CAG_nnn'CTG Cuts at: 2453 4833 6405 2453 Size: 2380 1572 3138 Fragments arranged by size: 3138 2380 1572 ApaI G_GGCC'C Cuts at: 4193 4193 Size: 7090 ApaLI G'TGCA_C Cuts at: 5147 6303 5147 Size: 1156 5934 ApoI r'AATT_y Cuts at: 1493 2527 2726 2901 4399 4625 4777 6886 1493 Size: 1034 199 175 1498 226 152 2109 1697 Fragments arranged by size: 2109 1697 1498 1034 226 199 175 152 AseI AT'TA_AT Cuts at: 160 1032 5644 160 Size: 872 4612 1606 Fragments arranged by size: 4612 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7090 AvaI C'yCGr_G Cuts at: 1870 1943 2187 2759 2802 3732 3787 3836 4184 Size: 73 244 572 43 930 55 49 348 Cuts at: 4184 4415 1870 Size: 231 4545 Fragments arranged by size: 4545 930 572 348 244 231 73 55 49 43 BamHI G'GATC_C Cuts at: 4420 4787 4420 Size: 367 6723 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2755 3443 3709 3727 4180 Size: 325 217 627 968 688 266 18 453 Cuts at: 4180 4223 4411 5866 618 Size: 43 188 1455 1842 Fragments arranged by size: 1842 1455 968 688 627 453 325 266 217 188 43 18 BanII G_rGCy'C Cuts at: 1391 2226 2628 2662 3837 4193 4409 1391 Size: 835 402 34 1175 356 216 4072 Fragments arranged by size: 4072 1175 835 402 356 216 34 BbeI G_GCGC'C Cuts at: 3713 3713 Size: 7090 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6119 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4068 4102 5281 5315 1903 Size: 34 2131 34 1179 34 3678 Fragments arranged by size: 3678 2131 1179 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3699 4982 6192 3699 Size: 1283 1210 4597 Fragments arranged by size: 4597 1283 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7090 BfrBI ATG'CAT Cuts at: 2203 2203 Size: 7090 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2147 3575 5701 Size: 107 122 71 631 605 475 1428 2126 Cuts at: 5701 136 Size: 1525 Fragments arranged by size: 2126 1525 1428 631 605 475 122 107 71 BglII A'GATC_T Cuts at: 7085 7085 Size: 7090 BlpI GC'TnA_GC Cuts at: 2627 3623 3890 4507 2627 Size: 996 267 617 5210 Fragments arranged by size: 5210 996 617 267 Bme1580I G_kGCm'C Cuts at: 1790 2760 3732 4185 4193 5151 6307 1790 Size: 970 972 453 8 958 1156 2573 Fragments arranged by size: 2573 1156 972 970 958 453 8 BmgBI CAC'GTC Cuts at: 1318 4216 1318 Size: 2898 4192 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4821 5779 442 Size: 416 53 83 399 24 3404 958 1753 Fragments arranged by size: 3404 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2021 1056 Size: 965 6125 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2101 2101 Size: 7090 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3043 5750 1239 Size: 1804 2707 2579 Fragments arranged by size: 2707 2579 1804 Bpu10I CC'TnA_GC Cuts at: 2622 2622 Size: 7090 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3806 5215 6080 6378 3806 Size: 1409 865 298 4518 Fragments arranged by size: 4518 1409 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3328 3517 5753 882 Size: 617 1829 189 2236 2219 Fragments arranged by size: 2236 2219 1829 617 189 BsaAI yAC'GTr Cuts at: 493 1204 3979 4881 493 Size: 711 2775 902 2702 Fragments arranged by size: 2775 2702 902 711 BsaBI GATnn'nnATC Cuts at: 7084 7084 Size: 7090 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3330 3660 3710 4897 Size: 53 83 186 936 1797 330 50 1187 Cuts at: 4897 5279 5914 275 Size: 382 635 1451 Fragments arranged by size: 1797 1451 1187 936 635 382 330 186 83 53 50 BsaWI w'CCGG_w Cuts at: 4268 4368 5523 5921 6195 6342 6753 4268 Size: 100 1155 398 274 147 411 4605 Fragments arranged by size: 4605 1155 411 398 274 147 100 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2273 2303 3596 3626 3983 Size: 30 165 30 870 30 1293 30 357 Cuts at: 3983 4013 1178 Size: 30 4255 Fragments arranged by size: 4255 1293 870 357 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2627 4162 1186 Size: 1441 1535 4114 Fragments arranged by size: 4114 1535 1441 BseYI C'CCAG_C Cuts at: 2442 4002 6293 6789 2442 Size: 1560 2291 496 2743 Fragments arranged by size: 2743 2291 1560 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2366 2628 3389 3959 4162 1163 Size: 573 630 262 761 570 203 4091 Fragments arranged by size: 4091 761 630 573 570 262 203 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3741 4167 4171 4540 5301 6329 Size: 275 186 1740 426 4 369 761 1028 Cuts at: 6329 1540 Size: 2301 Fragments arranged by size: 2301 1740 1028 761 426 369 275 186 4 BsiHKAI G_wGCw'C Cuts at: 2226 2441 2628 3306 3984 4119 5151 5236 6307 Size: 215 187 678 678 135 1032 85 1071 Cuts at: 6307 2226 Size: 3009 Fragments arranged by size: 3009 1071 1032 678 678 215 187 135 85 BsmI GAATG_Cn' Cuts at: 2205 4601 4694 2205 Size: 2396 93 4601 Fragments arranged by size: 4601 2396 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2226 2441 2628 2662 2760 3306 3732 Size: 399 436 215 187 34 98 546 426 Cuts at: 3732 3837 3984 4119 4185 4193 4409 5151 5236 Size: 105 147 135 66 8 216 742 85 Cuts at: 5236 6307 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 546 436 426 399 216 215 187 147 135 105 98 85 66 34 8 BspEI T'CCGG_A Cuts at: 4368 6753 4368 Size: 2385 4705 BspHI T'CATG_A Cuts at: 4979 6709 4979 Size: 1730 5360 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3501 4020 4445 844 Size: 2657 519 425 3489 Fragments arranged by size: 3489 2657 519 425 BsrBI CCG'CTC Cuts at: 1394 1823 2617 2801 4221 4977 1394 Size: 429 794 184 1420 756 3507 Fragments arranged by size: 3507 1420 794 756 429 184 BsrDI GCAATG_nn' Cuts at: 65 1248 5585 5759 65 Size: 1183 4337 174 1396 Fragments arranged by size: 4337 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2246 4283 5734 5921 1700 Size: 110 436 2037 1451 187 2869 Fragments arranged by size: 2869 2037 1451 436 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7090 BssHII G'CGCG_C Cuts at: 1925 2193 1925 Size: 268 6822 BssSI C'ACGA_G Cuts at: 5150 6162 5150 Size: 1012 6078 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7090 BstBI TT'CG_AA Cuts at: 5894 5894 Size: 7090 BstEII G'GTnAC_C Cuts at: 1184 3457 3757 1184 Size: 2273 300 4517 Fragments arranged by size: 4517 2273 300 BstXI CCAn_nnnn'nTGG Cuts at: 3769 4337 3769 Size: 568 6522 BstYI r'GATC_y Cuts at: 1972 4420 4787 5172 5189 6641 6727 6739 7085 Size: 2448 367 385 17 1452 86 12 346 Cuts at: 7085 1972 Size: 1977 Fragments arranged by size: 2448 1977 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3548 3548 Size: 7090 BtgI C'CryG_G Cuts at: 513 1065 2004 3607 3828 513 Size: 552 939 1603 221 3775 Fragments arranged by size: 3775 1603 939 552 221 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2871 487 Size: 683 56 1645 4706 Fragments arranged by size: 4706 1645 683 56 BtsI GCAGTG_nn' Cuts at: 2640 3421 4137 4606 5400 5420 2640 Size: 781 716 469 794 20 4310 Fragments arranged by size: 4310 794 781 716 469 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7090 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7055 DraI TTT'AAA Cuts at: 4394 4394 Size: 7090 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6097 809 Size: 507 4781 1802 Fragments arranged by size: 4781 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2139 2181 3532 Size: 54 1425 50 275 18 309 42 1351 Cuts at: 3532 3738 3906 3960 4537 5426 7055 8 Size: 206 168 54 577 889 1629 43 Fragments arranged by size: 1629 1425 1351 889 577 309 275 206 168 54 54 50 43 42 18 EagI C'GGCC_G Cuts at: 1537 1812 3738 4537 1537 Size: 275 1926 799 4090 Fragments arranged by size: 4090 1926 799 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4106 5020 1353 Size: 2753 914 3423 Fragments arranged by size: 3423 2753 914 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2652 5677 6051 6197 387 Size: 1064 138 1063 3025 374 146 1280 Fragments arranged by size: 3025 1280 1064 1063 374 146 138 EcoICRI GAG'CTC Cuts at: 2224 2626 2224 Size: 402 6688 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3043 4322 5153 5750 6537 6805 1239 Size: 393 1411 1279 831 597 787 268 1524 Fragments arranged by size: 1524 1411 1279 831 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2977 3568 4189 4190 4230 2977 Size: 591 621 1 40 5837 Fragments arranged by size: 5837 621 591 40 1 EcoRI G'AATT_C Cuts at: 4399 4777 4399 Size: 378 6712 FspI TGC'GCA Cuts at: 3875 5596 3875 Size: 1721 5369 FspAI rTGC'GCAy Cuts at: 3875 3875 Size: 7090 HaeII r_GCGC'y Cuts at: 1678 2603 3426 3623 3638 3713 4338 6237 1678 Size: 925 823 197 15 75 625 1899 2531 Fragments arranged by size: 2531 1899 925 823 625 197 75 15 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3107 3139 3422 3454 3596 Size: 32 135 32 1535 32 283 32 142 Cuts at: 3596 3628 3770 3802 3983 4015 5731 5763 5805 Size: 32 142 32 181 32 1716 32 42 Cuts at: 5805 5837 1373 Size: 32 2626 Fragments arranged by size: 2626 1716 1535 283 181 142 142 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4434 4680 669 Size: 988 281 2496 246 3079 Fragments arranged by size: 3079 2496 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7090 HpaI GTT'AAC Cuts at: 4680 4680 Size: 7090 KasI G'GCGC_C Cuts at: 3709 3709 Size: 7090 KpnI G_GTAC'C Cuts at: 4415 4415 Size: 7090 MfeI C'AATT_G Cuts at: 4689 4689 Size: 7090 MluI A'CGCG_T Cuts at: 6966 6966 Size: 7090 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3351 6204 6388 1305 Size: 2046 2853 184 2007 Fragments arranged by size: 2853 2046 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2141 2183 10 Size: 54 1425 343 309 42 4917 Fragments arranged by size: 4917 1425 343 309 54 42 MslI CAynn'nnrTG Cuts at: 518 1166 2538 3209 3275 5048 5407 5566 518 Size: 648 1372 671 66 1773 359 159 2042 Fragments arranged by size: 2042 1773 1372 671 648 359 159 66 MspA1I CmG'CkG Cuts at: 1606 3609 3694 3817 4018 4506 5183 6331 6576 Size: 2003 85 123 201 488 677 1148 245 Cuts at: 6576 1606 Size: 2120 Fragments arranged by size: 2120 2003 1148 677 488 245 201 123 85 NaeI GCC'GGC Cuts at: 4285 4285 Size: 7090 NarI GG'CG_CC Cuts at: 3710 3710 Size: 7090 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5599 Fragments arranged by size: 5599 939 552 NdeI CA'TA_TG Cuts at: 387 2824 387 Size: 2437 4653 NgoMIV G'CCGG_C Cuts at: 4283 4283 Size: 7090 NheI G'CTAG_C Cuts at: 1052 2017 1052 Size: 965 6125 NotI GC'GGCC_GC Cuts at: 4537 4537 Size: 7090 NsiI A_TGCA'T Cuts at: 2205 2205 Size: 7090 NspI r_CATG'y Cuts at: 1132 1229 3853 4448 5993 1132 Size: 97 2624 595 1545 2229 Fragments arranged by size: 2624 2229 1545 595 97 PasI CC'CwG_GG Cuts at: 1348 4330 1348 Size: 2982 4108 PciI A'CATG_T Cuts at: 5989 5989 Size: 7090 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7090 PfoI T'CCnGG_A Cuts at: 2672 2672 Size: 7090 PmeI GTTT'AAAC Cuts at: 4394 4394 Size: 7090 PmlI CAC'GTG Cuts at: 3979 3979 Size: 7090 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4280 4312 5159 5191 6672 6704 4280 Size: 32 847 32 1481 32 4666 Fragments arranged by size: 4666 1481 847 32 32 32 PpuMI rG'GwC_Cy Cuts at: 4230 4230 Size: 7090 PshAI GACnn'nnGTC Cuts at: 1613 2160 1613 Size: 547 6543 PsiI TTA'TAA Cuts at: 2946 4660 2946 Size: 1714 5376 PspOMI G'GGCC_C Cuts at: 4189 4189 Size: 7090 PstI C_TGCA'G Cuts at: 830 2370 3576 3699 4442 830 Size: 1540 1206 123 743 3478 Fragments arranged by size: 3478 1540 1206 743 123 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7090 PvuII CAG'CTG Cuts at: 3817 3817 Size: 7090 SacI G_AGCT'C Cuts at: 2226 2628 2226 Size: 402 6688 SacII CC_GC'GG Cuts at: 3610 3610 Size: 7090 SalI G'TCGA_C Cuts at: 1936 4432 1936 Size: 2496 4594 SanDI GG'GwC_CC Cuts at: 4230 4230 Size: 7090 SapI GCTCTTCn'nnn_ Cuts at: 4106 4106 Size: 7090 SbfI CC_TGCA'GG Cuts at: 3576 4442 3576 Size: 866 6224 ScaI AGT'ACT Cuts at: 5338 7050 5338 Size: 1712 5378 SexAI A'CCwGG_T Cuts at: 3753 4068 3753 Size: 315 6775 SfcI C'TryA_G Cuts at: 826 1045 2366 3003 3090 3572 3695 4438 5573 Size: 219 1321 637 87 482 123 743 1135 Cuts at: 5573 6254 6445 826 Size: 681 191 1471 Fragments arranged by size: 1471 1321 1135 743 681 637 482 219 191 123 87 SfoI GGC'GCC Cuts at: 3711 3711 Size: 7090 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7090 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7090 SmaI CCC'GGG Cuts at: 1872 3734 3838 4417 1872 Size: 1862 104 579 4545 Fragments arranged by size: 4545 1862 579 104 SmlI C'TyrA_G Cuts at: 820 1017 1943 2802 3821 5194 6095 6357 820 Size: 197 926 859 1019 1373 901 262 1553 Fragments arranged by size: 1553 1373 1019 926 901 859 262 197 SnaBI TAC'GTA Cuts at: 493 4881 493 Size: 4388 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7090 SphI G_CATG'C Cuts at: 1132 4448 1132 Size: 3316 3774 SrfI GCCC'GGGC Cuts at: 3838 3838 Size: 7090 SspI AAT'ATT Cuts at: 5 52 5014 5 Size: 47 4962 2081 Fragments arranged by size: 4962 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7090 StyI C'CwwG_G Cuts at: 513 1065 2004 2851 3762 4529 513 Size: 552 939 847 911 767 3074 Fragments arranged by size: 3074 939 911 847 767 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2456 3301 3371 4157 5117 5134 5287 Size: 1255 876 845 70 786 960 17 153 Cuts at: 5287 5472 5917 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 960 876 845 786 445 185 153 70 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3249 5336 7048 Size: 274 80 33 51 1443 1272 2087 1712 Cuts at: 7048 96 Size: 138 Fragments arranged by size: 2087 1712 1443 1272 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2273 2305 7050 7082 2273 Size: 32 4745 32 2281 Fragments arranged by size: 4745 2281 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7090 XbaI T'CTAG_A Cuts at: 4426 4426 Size: 7090 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3916 3916 Size: 7090 XhoI C'TCGA_G Cuts at: 1943 2802 1943 Size: 859 6231 XmaI C'CCGG_G Cuts at: 1870 3732 3836 4415 1870 Size: 1862 104 579 4545 Fragments arranged by size: 4545 1862 579 104 XmnI GAAnn'nnTTC Cuts at: 5219 5219 Size: 7090 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3331 4898 276 Size: 53 83 186 936 1797 1567 2468 Fragments arranged by size: 2468 1797 1567 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbvCI BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FalI FseI NruI PacI PspXI PsrI RsrII SfiI SwaI