Restriction map for
pFN21AB4336
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# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII AccIII AclI AcsI AgeI
Alw44I Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I AviII AvrII
BamHI BcgI BciVI BclI BglII
BlnI BlpI Bpu1102I BsaBI BseAI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspEI BspLU11I BspMI BsrDI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I CelII ClaI CpoI DraI
DrdI EagI EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HpaI KspI MamI
MfeI MroI MunI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflMI PinAI PmeI
PshBI Psp1406I PvuI RsrII SacII
SapI SbfI SexAI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Van91I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 29 enzymes) #
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Acc65I 3022 GGTACC G^GTACC
AflII 742 CTTAAG C^TTAAG
AocI 1987 CCTCAGG CC^T[ACGT]AGG
Asp718I 3022 GGTACC G^GTACC
BalI 2978 TGGCCA TGG^CCA
BbeI 1260 GGCGCC GGCGC^C
BbrPI 253 CACGTG CAC^GTG
BfrI 742 CTTAAG C^TTAAG
BsaWI 1967 TCCGGT [AT]^CCGG[AT]
BsmI 1069 GCATTC GAATGC(1/-1)
BspHI 336 TCATGA T^CATGA
Bsu36I 1987 CCTCAGG CC^T[ACGT]AGG
Eco81I 1987 CCTCAGG CC^T[ACGT]AGG
EcoNI 1375 CCTGAGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
KasI 1260 GGCGCC G^GCGCC
KpnI 3022 GGTACC GGTAC^C
MluI 83 ACGCGT A^CGCGT
MluNI 2978 TGGCCA TGG^CCA
MscI 2978 TGGCCA TGG^CCA
NarI 1260 GGCGCC GG^CGCC
PmaCI 253 CACGTG CAC^GTG
PmlI 253 CACGTG CAC^GTG
PshAI 2892 GACTGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 336 TCATGA T^CATGA
SacI 1050 GAGCTC GAGCT^C
SalI 434 GTCGAC G^TCGAC
SfoI 1260 GGCGCC GGC^GCC
StuI 1383 AGGCCT AGG^CCT
Tsp509I 287 AATT ^AATT
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# TWO CUT ENZYMES ( 34 enzymes) #
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AccII 84 CGCG CG^CG
2181 CGCG
AcyI 1260 GGCGCC G[GA]^CG[CT]C
2146 GGCGTC
AflIII 83 ACGCGT A^C[GA][CT]GT
252 ACACGT
AhdI 1276 GACTATAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3158 GACCTCCAGTC
AspEI 1276 GACTATAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3158 GACCTCCAGTC
AspI 505 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
814 GACAAGGTC
BfaI 1642 CTAG C^TAG
3079 CTAG
BsaAI 253 CACGTG [CT]AC^GT[GA]
328 TACGTG
BsaHI 1260 GGCGCC G[GA]^CG[CT]C
2146 GGCGTC
BsrBI 1776 CCGCTC CCGCTC(-3/-3)
2242 GAGCGG
BssSI 700 CACGAG CACGAG(-5/-1)
850 CACGAG
BstUI 84 CGCG CG^CG
2181 CGCG
BstYI 2216 AGATCC [GA]^GATC[CT]
2918 AGATCC
Eam1105I 1276 GACTATAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3158 GACCTCCAGTC
HgaI 2147 GCGTC GACGC(5/10)
3046 GCGTC
Hin1I 1260 GGCGCC G[GA]^CG[CT]C
2146 GGCGTC
HincII 434 GTCGAC GT[CT]^[GA]AC
2149 GTCAAC
HindII 434 GTCGAC GT[CT]^[GA]AC
2149 GTCAAC
MaeI 1642 CTAG C^TAG
3079 CTAG
MflI 2216 AGATCC [GA]^GATC[CT]
2918 AGATCC
MseI 743 TTAA T^TAA
1904 TTAA
MvnI 84 CGCG CG^CG
2181 CGCG
NaeI 2143 GCCGGC GCC^GGC
2353 GCCGGC
NgoMIV 2143 GCCGGC G^CCGGC
2353 GCCGGC
PflFI 505 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
814 GACAAGGTC
ScaI 515 AGTACT AGT^ACT
1103 AGTACT
SmaI 1256 CCCGGG CCC^GGG
2608 CCCGGG
TfiI 163 GAATC G^A[AT]TC
2253 GATTC
Tru9I 743 TTAA T^TAA
1904 TTAA
Tsp45I 451 GTCAC ^GT[GC]AC
1294 GTCAC
Tth111I 505 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
814 GACAAGGTC
XhoII 2216 AGATCC [GA]^GATC[CT]
2918 AGATCC
XmaCI 1256 CCCGGG C^CCGGG
2608 CCCGGG
XmaI 1256 CCCGGG C^CCGGG
2608 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AccI AciI AfaI AluI AlwI
AlwNI ApaI AspHI AvaI AvaII
BanI BanII BbsI BbvCI BbvI
BcnI BglI BmyI BpmI BpuAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstXI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MnlI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TseI
TspRI TthHB8I XcmI