(Circular) (Six-base) MAPSORT of: pFN21AB4336.seq Check: 436 from: 1 to: 7984 pFN21AB4336 7984 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5794 278 Size: 53 83 186 936 4258 2468 Fragments arranged by size: 4258 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2073 2440 4905 5327 1937 Size: 136 367 2465 422 4594 Fragments arranged by size: 4594 2465 422 367 136 Acc65I G'GTAC_C Cuts at: 5027 5305 5027 Size: 278 7706 AclI AA'CG_TT Cuts at: 1563 6111 6484 1563 Size: 4548 373 3063 Fragments arranged by size: 4548 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2530 3120 3413 4193 4808 6047 7431 7699 Size: 898 590 293 780 615 1239 1384 268 Cuts at: 7699 1632 Size: 1917 Fragments arranged by size: 1917 1384 1239 898 780 615 590 293 268 AflII C'TTAA_G Cuts at: 820 1017 2747 820 Size: 197 1730 6057 Fragments arranged by size: 6057 1730 197 AflIII A'CryG_T Cuts at: 1203 2088 2257 6883 7860 1203 Size: 885 169 4626 977 1327 Fragments arranged by size: 4626 1327 977 885 169 AgeI A'CCGG_T Cuts at: 6815 6815 Size: 7984 AhdI GACnn_n'nnGTC Cuts at: 3286 5168 6713 3286 Size: 1882 1545 4557 Fragments arranged by size: 4557 1882 1545 AleI CACnn'nnGTG Cuts at: 1166 3359 3836 1166 Size: 2193 477 5314 Fragments arranged by size: 5314 2193 477 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4551 1184 Size: 3367 4617 AlwNI CAG_nnn'CTG Cuts at: 4058 4123 4210 4225 4864 4951 5054 5132 5727 Size: 65 87 15 639 87 103 78 595 Cuts at: 5727 7299 4058 Size: 1572 4743 Fragments arranged by size: 4743 1572 639 595 103 87 87 78 65 15 ApaI G_GGCC'C Cuts at: 3027 3219 3262 3482 3750 4338 4567 4765 3027 Size: 192 43 220 268 588 229 198 6246 Fragments arranged by size: 6246 588 268 229 220 198 192 43 ApaLI G'TGCA_C Cuts at: 6041 7197 6041 Size: 1156 6828 ApoI r'AATT_y Cuts at: 1493 5293 5519 5671 7780 1493 Size: 3800 226 152 2109 1697 Fragments arranged by size: 3800 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6538 160 Size: 872 5506 1606 Fragments arranged by size: 5506 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7984 AvaI C'yCGr_G Cuts at: 1870 1943 2282 2404 3161 3261 4465 4613 5309 Size: 73 339 122 757 100 1204 148 696 Cuts at: 5309 1870 Size: 4545 Fragments arranged by size: 4545 1204 757 696 339 148 122 100 73 BamHI G'GATC_C Cuts at: 5314 5681 5314 Size: 367 7617 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2134 3265 3323 3902 4609 Size: 325 217 627 347 1131 58 579 707 Cuts at: 4609 5027 5305 6760 618 Size: 418 278 1455 1842 Fragments arranged by size: 1842 1455 1131 707 627 579 418 347 325 278 217 58 BanII G_rGCy'C Cuts at: 1391 2319 3027 3059 3168 3219 3262 3482 3750 Size: 928 708 32 109 51 43 220 268 Cuts at: 3750 4338 4394 4535 4567 4701 4765 4970 5083 Size: 588 56 141 32 134 64 205 113 Cuts at: 5083 5303 1391 Size: 220 4072 Fragments arranged by size: 4072 928 708 588 268 220 220 205 141 134 113 109 64 56 51 43 32 32 BbeI G_GCGC'C Cuts at: 3269 3269 Size: 7984 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2564 2904 3193 3999 928 Size: 971 665 340 289 806 4913 Fragments arranged by size: 4913 971 806 665 340 289 BbvCI CC'TCA_GC Cuts at: 3154 3776 4647 5198 5263 3154 Size: 622 871 551 65 5875 Fragments arranged by size: 5875 871 622 551 65 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6175 6209 1903 Size: 34 4238 34 3678 Fragments arranged by size: 4238 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5876 7086 5876 Size: 1210 6774 BclI T'GATC_A Cuts at: 1548 1548 Size: 7984 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2471 3512 4755 Size: 107 122 71 631 605 799 1041 1243 Cuts at: 4755 5087 6595 136 Size: 332 1508 1525 Fragments arranged by size: 1525 1508 1243 1041 799 631 605 332 122 107 71 BglII A'GATC_T Cuts at: 7979 7979 Size: 7984 BlpI GC'TnA_GC Cuts at: 5401 5401 Size: 7984 Bme1580I G_kGCm'C Cuts at: 1790 2088 2210 2257 2272 2467 3027 3219 3262 Size: 298 122 47 15 195 560 192 43 Cuts at: 3262 3473 3482 3508 3750 4284 4338 4567 4612 Size: 211 9 26 242 534 54 229 45 Cuts at: 4612 4765 6045 7201 1790 Size: 153 1280 1156 2573 Fragments arranged by size: 2573 1280 1156 560 534 298 242 229 211 195 192 153 122 54 47 45 43 26 15 9 BmgBI CAC'GTC Cuts at: 1318 2455 3298 1318 Size: 1137 843 6004 Fragments arranged by size: 6004 1137 843 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3483 3495 5715 Size: 416 53 83 399 24 2066 12 2220 Cuts at: 5715 6673 442 Size: 958 1753 Fragments arranged by size: 2220 2066 1753 958 416 399 83 53 24 12 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7984 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2522 2619 3107 3229 3570 3653 3718 3977 Size: 1283 97 488 122 341 83 65 259 Cuts at: 3977 4145 4508 4829 4838 5097 5151 6644 1239 Size: 168 363 321 9 259 54 1493 2579 Fragments arranged by size: 2579 1493 1283 488 363 341 321 259 259 168 122 97 83 65 54 9 Bpu10I CC'TnA_GC Cuts at: 3154 3250 3776 4226 4647 5198 5263 3154 Size: 96 526 450 421 551 65 5875 Fragments arranged by size: 5875 551 526 450 421 96 65 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3646 4396 4765 6109 6974 7272 3646 Size: 750 369 1344 865 298 4358 Fragments arranged by size: 4358 1344 865 750 369 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2830 2867 3368 6647 882 Size: 617 1331 37 501 3279 2219 Fragments arranged by size: 3279 2219 1331 617 501 37 BsaAI yAC'GTr Cuts at: 493 1204 2260 2335 5775 493 Size: 711 1056 75 3440 2702 Fragments arranged by size: 3440 2702 1056 711 75 BsaBI GATnn'nnATC Cuts at: 7978 7978 Size: 7984 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3266 4152 5791 6173 Size: 53 83 186 936 1733 886 1639 382 Cuts at: 6173 6808 275 Size: 635 1451 Fragments arranged by size: 1733 1639 1451 936 886 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3972 6417 6815 7089 7236 7647 3972 Size: 2445 398 274 147 411 4309 Fragments arranged by size: 4309 2445 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2810 2840 3092 3095 3122 Size: 30 165 30 1407 30 252 3 27 Cuts at: 3122 3125 3668 3698 3989 4019 5070 5100 1178 Size: 3 543 30 291 30 1051 30 4062 Fragments arranged by size: 4062 1407 1051 543 291 252 165 30 30 30 30 30 30 27 3 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2390 2604 2616 2619 2665 2818 2821 2977 Size: 1204 214 12 3 46 153 3 156 Cuts at: 2977 2989 2992 3001 3004 3113 3436 3721 3799 Size: 12 3 9 3 109 323 285 78 Cuts at: 3799 3908 4511 1186 Size: 109 603 4659 Fragments arranged by size: 4659 1204 603 323 285 214 156 153 109 109 78 46 12 12 9 3 3 3 3 BseYI C'CCAG_C Cuts at: 2052 2250 2783 3481 3507 3620 4584 4627 4961 Size: 198 533 698 26 113 964 43 334 Cuts at: 4961 4971 7187 7683 2052 Size: 10 2216 496 2353 Fragments arranged by size: 2353 2216 964 698 533 496 334 198 113 43 26 10 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2113 4044 4166 4289 1163 Size: 573 377 1931 122 123 4858 Fragments arranged by size: 4858 1931 573 377 123 122 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5434 6195 7223 1540 Size: 275 186 3433 761 1028 2301 Fragments arranged by size: 3433 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2307 3059 4382 6045 6130 7201 2307 Size: 752 1323 1663 85 1071 3090 Fragments arranged by size: 3090 1663 1323 1071 752 85 BsmI GAATG_Cn' Cuts at: 3074 5495 5588 3074 Size: 2421 93 5470 Fragments arranged by size: 5470 2421 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2088 2210 2257 2272 2307 2319 2467 Size: 399 298 122 47 15 35 12 148 Cuts at: 2467 3027 3059 3168 3219 3262 3473 3482 3508 Size: 560 32 109 51 43 211 9 26 Cuts at: 3508 3750 4284 4338 4382 4394 4535 4567 4612 Size: 242 534 54 44 12 141 32 45 Cuts at: 4612 4701 4765 4970 5083 5303 6045 6130 7201 Size: 89 64 205 113 220 742 85 1071 Cuts at: 7201 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 560 534 399 298 242 220 211 205 148 141 122 113 109 89 85 64 54 51 47 45 44 43 35 32 32 26 15 12 12 9 BspEI T'CCGG_A Cuts at: 7647 7647 Size: 7984 BspHI T'CATG_A Cuts at: 2341 5873 7603 2341 Size: 3532 1730 2722 Fragments arranged by size: 3532 2722 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5339 844 Size: 4495 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3783 4249 5871 1394 Size: 429 1960 466 1622 3507 Fragments arranged by size: 3507 1960 1622 466 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6479 6653 65 Size: 1183 5231 174 1396 Fragments arranged by size: 5231 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2658 4148 4358 6628 6815 1700 Size: 110 848 1490 210 2270 187 2869 Fragments arranged by size: 2869 2270 1490 848 210 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7984 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7984 BssSI C'ACGA_G Cuts at: 2705 2855 6044 7056 2705 Size: 150 3189 1012 3633 Fragments arranged by size: 3633 3189 1012 150 BstAPI GCAn_nnn'nTGC Cuts at: 794 2335 3350 4189 4951 5132 794 Size: 1541 1015 839 762 181 3646 Fragments arranged by size: 3646 1541 1015 839 762 181 BstBI TT'CG_AA Cuts at: 6788 6788 Size: 7984 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7984 BstXI CCAn_nnnn'nTGG Cuts at: 3515 3742 3887 4642 4993 3515 Size: 227 145 755 351 6506 Fragments arranged by size: 6506 755 351 227 145 BstYI r'GATC_y Cuts at: 1972 4221 4923 5314 5681 6066 6083 7535 7621 Size: 2249 702 391 367 385 17 1452 86 Cuts at: 7621 7633 7979 1972 Size: 12 346 1977 Fragments arranged by size: 2249 1977 1452 702 391 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3993 3993 Size: 7984 BtgI C'CryG_G Cuts at: 513 1065 2310 2431 2802 3430 3897 4641 4980 Size: 552 1245 121 371 628 467 744 339 Cuts at: 4980 513 Size: 3517 Fragments arranged by size: 3517 1245 744 628 552 467 371 339 121 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2094 487 Size: 683 56 868 6377 Fragments arranged by size: 6377 868 683 56 BtsI GCAGTG_nn' Cuts at: 2638 2811 2959 3528 4681 5175 5227 5500 6294 Size: 173 148 569 1153 494 52 273 794 Cuts at: 6294 6314 2638 Size: 20 4308 Fragments arranged by size: 4308 1153 794 569 494 273 173 148 52 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7984 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2096 2131 529 Size: 35 1532 35 6382 Fragments arranged by size: 6382 1532 35 35 DraI TTT'AAA Cuts at: 5288 5288 Size: 7984 DraIII CAC_nnn'GTG Cuts at: 2263 3350 4067 2263 Size: 1087 717 6180 Fragments arranged by size: 6180 1087 717 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6991 809 Size: 507 5675 1802 Fragments arranged by size: 5675 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2100 4075 4236 Size: 54 1425 50 275 18 270 1975 161 Cuts at: 4236 4983 5121 5431 6320 7949 8 Size: 747 138 310 889 1629 43 Fragments arranged by size: 1975 1629 1425 889 747 310 275 270 161 138 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5431 1537 Size: 275 3619 4090 Fragments arranged by size: 4090 3619 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2225 2717 2848 2995 3010 3817 4046 5914 Size: 872 492 131 147 15 807 229 1868 Cuts at: 5914 1353 Size: 3423 Fragments arranged by size: 3423 1868 872 807 492 229 147 131 15 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2601 2971 5175 6571 6945 7091 Size: 1064 138 1012 370 2204 1396 374 146 Cuts at: 7091 387 Size: 1280 Fragments arranged by size: 2204 1396 1280 1064 1012 374 370 146 138 EcoICRI GAG'CTC Cuts at: 3057 3057 Size: 7984 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2522 2530 2619 3107 3120 3229 3413 Size: 393 890 8 89 488 13 109 184 Cuts at: 3413 3570 3653 3718 3977 4145 4193 4508 4808 Size: 157 83 65 259 168 48 315 300 Cuts at: 4808 4829 4838 5097 5151 6047 6644 7431 7699 Size: 21 9 259 54 896 597 787 268 Cuts at: 7699 1239 Size: 1524 Fragments arranged by size: 1524 896 890 787 597 488 393 315 300 268 259 259 184 168 157 109 89 83 65 54 48 21 13 9 8 EcoNI CCTnn'n_nnAGG Cuts at: 3384 3384 Size: 7984 EcoO109I rG'GnC_Cy Cuts at: 2144 3023 3024 3312 3478 3676 3746 3747 3756 Size: 879 1 288 166 198 70 1 9 Cuts at: 3756 3984 4335 4447 4563 4564 4681 4761 4762 Size: 228 351 112 116 1 117 80 1 Cuts at: 4762 2144 Size: 5366 Fragments arranged by size: 5366 879 351 288 228 198 166 117 116 112 80 70 9 1 1 1 1 EcoRI G'AATT_C Cuts at: 5293 5671 5293 Size: 378 7606 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2762 3217 2762 Size: 455 7529 FspI TGC'GCA Cuts at: 6490 6490 Size: 7984 HaeII r_GCGC'y Cuts at: 1678 2598 3269 4181 4951 7131 1678 Size: 920 671 912 770 2180 2531 Fragments arranged by size: 2531 2180 920 912 770 671 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2810 2842 3095 3127 3272 Size: 32 135 32 1238 32 253 32 145 Cuts at: 3272 3304 3668 3700 3976 3989 4008 4021 4439 Size: 32 364 32 276 13 19 13 418 Cuts at: 4439 4471 5154 5186 6625 6657 6699 6731 1373 Size: 32 683 32 1439 32 42 32 2626 Fragments arranged by size: 2626 1439 1238 683 418 364 276 253 145 135 42 32 32 32 32 32 32 32 32 32 32 19 13 13 HincII GTy'rAC Cuts at: 669 1657 1938 2441 4156 5328 5574 669 Size: 988 281 503 1715 1172 246 3079 Fragments arranged by size: 3079 1715 1172 988 503 281 246 HindIII A'AGCT_T Cuts at: 748 2112 2445 4741 748 Size: 1364 333 2296 3991 Fragments arranged by size: 3991 2296 1364 333 HpaI GTT'AAC Cuts at: 5574 5574 Size: 7984 KasI G'GCGC_C Cuts at: 3265 3265 Size: 7984 KpnI G_GTAC'C Cuts at: 5031 5309 5031 Size: 278 7706 MfeI C'AATT_G Cuts at: 5583 5583 Size: 7984 MluI A'CGCG_T Cuts at: 2088 7860 2088 Size: 5772 2212 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 7098 7282 1305 Size: 5793 184 2007 Fragments arranged by size: 5793 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4985 10 Size: 54 1425 343 3153 3009 Fragments arranged by size: 3153 3009 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3359 3836 4640 5942 6301 6460 518 Size: 648 2193 477 804 1302 359 159 2042 Fragments arranged by size: 2193 2042 1302 804 648 477 359 159 MspA1I CmG'CkG Cuts at: 1606 2068 2577 3485 3511 4207 4513 4622 4724 Size: 462 509 908 26 696 306 109 102 Cuts at: 4724 4777 4879 4975 5014 5075 5132 5138 5249 Size: 53 102 96 39 61 57 6 111 Cuts at: 5249 5400 6077 7225 7470 1606 Size: 151 677 1148 245 2120 Fragments arranged by size: 2120 1148 908 696 677 509 462 306 245 151 111 109 102 102 96 61 57 53 39 26 6 NaeI GCC'GGC Cuts at: 4150 4360 4150 Size: 210 7774 NarI GG'CG_CC Cuts at: 3266 3266 Size: 7984 NcoI C'CATG_G Cuts at: 513 1065 2310 2431 3430 3897 4641 4980 513 Size: 552 1245 121 999 467 744 339 3517 Fragments arranged by size: 3517 1245 999 744 552 467 339 121 NdeI CA'TA_TG Cuts at: 387 387 Size: 7984 NgoMIV G'CCGG_C Cuts at: 4148 4358 4148 Size: 210 7774 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7984 NotI GC'GGCC_GC Cuts at: 5431 5431 Size: 7984 NspI r_CATG'y Cuts at: 1132 1229 5342 6887 1132 Size: 97 4113 1545 2229 Fragments arranged by size: 4113 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3275 3752 4097 4331 1348 Size: 1927 477 345 234 5001 Fragments arranged by size: 5001 1927 477 345 234 PciI A'CATG_T Cuts at: 6883 6883 Size: 7984 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7984 PfoI T'CCnGG_A Cuts at: 4396 4396 Size: 7984 PmeI GTTT'AAAC Cuts at: 5288 5288 Size: 7984 PmlI CAC'GTG Cuts at: 2260 2260 Size: 7984 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6053 6085 7566 7598 6053 Size: 32 1481 32 6439 Fragments arranged by size: 6439 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2144 3676 3756 3984 4447 2144 Size: 1532 80 228 463 5681 Fragments arranged by size: 5681 1532 463 228 80 PshAI GACnn'nnGTC Cuts at: 1613 4901 1613 Size: 3288 4696 PsiI TTA'TAA Cuts at: 5554 5554 Size: 7984 PspOMI G'GGCC_C Cuts at: 3023 3215 3258 3478 3746 4334 4563 4761 3023 Size: 192 43 220 268 588 229 198 6246 Fragments arranged by size: 6246 588 268 229 220 198 192 43 PstI C_TGCA'G Cuts at: 830 2331 3040 3054 3719 3968 4194 4257 4776 Size: 1501 709 14 665 249 226 63 519 Cuts at: 4776 4947 4956 5137 5143 5336 830 Size: 171 9 181 6 193 3478 Fragments arranged by size: 3478 1501 709 665 519 249 226 193 181 171 63 14 9 6 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7984 PvuII CAG'CTG Cuts at: 3485 3511 4777 4879 4975 5132 5138 3485 Size: 26 1266 102 96 157 6 6331 Fragments arranged by size: 6331 1266 157 102 96 26 6 SacI G_AGCT'C Cuts at: 3059 3059 Size: 7984 SalI G'TCGA_C Cuts at: 1936 2439 5326 1936 Size: 503 2887 4594 Fragments arranged by size: 4594 2887 503 SanDI GG'GwC_CC Cuts at: 2144 2144 Size: 7984 SbfI CC_TGCA'GG Cuts at: 5336 5336 Size: 7984 ScaI AGT'ACT Cuts at: 2522 3110 6232 7944 2522 Size: 588 3122 1712 2562 Fragments arranged by size: 3122 2562 1712 588 SfcI C'TryA_G Cuts at: 826 1045 2327 3036 3050 3715 3964 4190 4253 Size: 219 1282 709 14 665 249 226 63 Cuts at: 4253 4772 4943 4952 5133 5139 5332 6467 7148 Size: 519 171 9 181 6 193 1135 681 Cuts at: 7148 7339 826 Size: 191 1471 Fragments arranged by size: 1471 1282 1135 709 681 665 519 249 226 219 193 191 181 171 63 14 9 6 SfoI GGC'GCC Cuts at: 3267 3267 Size: 7984 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7984 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7984 SmaI CCC'GGG Cuts at: 1872 3263 4615 5311 1872 Size: 1391 1352 696 4545 Fragments arranged by size: 4545 1391 1352 696 SmlI C'TyrA_G Cuts at: 820 1017 1943 2747 3625 4375 4744 6088 6989 Size: 197 926 804 878 750 369 1344 901 Cuts at: 6989 7251 820 Size: 262 1553 Fragments arranged by size: 1553 1344 926 901 878 804 750 369 262 197 SnaBI TAC'GTA Cuts at: 493 5775 493 Size: 5282 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7984 SphI G_CATG'C Cuts at: 1132 5342 1132 Size: 4210 3774 SrfI GCCC'GGGC Cuts at: 3263 3263 Size: 7984 SspI AAT'ATT Cuts at: 5 52 5908 5 Size: 47 5856 2081 Fragments arranged by size: 5856 2081 47 StuI AGG'CCT Cuts at: 1517 3390 1517 Size: 1873 6111 StyI C'CwwG_G Cuts at: 513 1065 2310 2431 3171 3430 3447 3897 4641 Size: 552 1245 121 740 259 17 450 744 Cuts at: 4641 4702 4980 5423 513 Size: 61 278 443 3074 Fragments arranged by size: 3074 1245 744 740 552 450 443 278 259 121 61 17 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2714 3341 3890 6011 6028 6181 6366 Size: 1255 1134 627 549 2121 17 153 185 Cuts at: 6366 6811 325 Size: 445 1498 Fragments arranged by size: 2121 1498 1255 1134 627 549 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2520 3108 6230 Size: 274 80 33 51 1443 543 588 3122 Cuts at: 6230 7942 96 Size: 1712 138 Fragments arranged by size: 3122 1712 1443 588 543 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5070 5102 7944 7976 5070 Size: 32 2842 32 5078 Fragments arranged by size: 5078 2842 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2513 2822 1316 Size: 1197 309 6478 Fragments arranged by size: 6478 1197 309 XbaI T'CTAG_A Cuts at: 5320 5320 Size: 7984 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2117 2145 3508 3628 4546 2011 Size: 106 28 1363 120 918 5449 Fragments arranged by size: 5449 1363 918 120 106 28 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7984 XmaI C'CCGG_G Cuts at: 1870 3261 4613 5309 1870 Size: 1391 1352 696 4545 Fragments arranged by size: 4545 1391 1352 696 XmnI GAAnn'nnTTC Cuts at: 6113 6113 Size: 7984 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5792 276 Size: 53 83 186 936 4258 2468 Fragments arranged by size: 4258 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I EcoRV FseI FspAI NruI NsiI PacI PspXI PsrI RsrII SacII SapI SexAI SfiI SwaI