Restriction map for
pFN21AB4336
(Linear) (Six-base) MAPSORT of: KIBB4336.seq Check: 4427 from: 1 to: 3279
KIBB4336.orf
With 182 enzymes: SgfI *
July 18, 2007 13:37 ..
AccI GT'mk_AC
Cuts at: 0 68 435 2900 3279
Size: 68 367 2465 379
Fragments arranged by size:
2465 379 367 68
Acc65I G'GTAC_C
Cuts at: 0 3022 3279
Size: 3022 257
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 525 1115 1408 2188 2803 3279
Size: 525 590 293 780 615 476
Fragments arranged by size:
780 615 590 525 476 293
AflII C'TTAA_G
Cuts at: 0 742 3279
Size: 742 2537
AflIII A'CryG_T
Cuts at: 0 83 252 3279
Size: 83 169 3027
Fragments arranged by size:
3027 169 83
AhdI GACnn_n'nnGTC
Cuts at: 0 1281 3163 3279
Size: 1281 1882 116
Fragments arranged by size:
1882 1281 116
AleI CACnn'nnGTG
Cuts at: 0 1354 1831 3279
Size: 1354 477 1448
Fragments arranged by size:
1448 1354 477
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2546 3279
Size: 2546 733
AlwNI CAG_nnn'CTG
Cuts at: 0 2053 2118 2205 2220 2859 2946 3049 3127
Size: 2053 65 87 15 639 87 103 78
Cuts at: 3127 3279
Size: 152
Fragments arranged by size:
2053 639 152 103 87 87 78 65
15
ApaI G_GGCC'C
Cuts at: 0 1022 1214 1257 1477 1745 2333 2562 2760
Size: 1022 192 43 220 268 588 229 198
Cuts at: 2760 3279
Size: 519
Fragments arranged by size:
1022 588 519 268 229 220 198 192
43
AvaI C'yCGr_G
Cuts at: 0 277 399 1156 1256 2460 2608 3279
Size: 277 122 757 100 1204 148 671
Fragments arranged by size:
1204 757 671 277 148 122 100
BanI G'GyrC_C
Cuts at: 0 129 1260 1318 1897 2604 3022 3279
Size: 129 1131 58 579 707 418 257
Fragments arranged by size:
1131 707 579 418 257 129 58
BanII G_rGCy'C
Cuts at: 0 314 1022 1054 1163 1214 1257 1477 1745
Size: 314 708 32 109 51 43 220 268
Cuts at: 1745 2333 2389 2530 2562 2696 2760 2965 3078
Size: 588 56 141 32 134 64 205 113
Cuts at: 3078 3279
Size: 201
Fragments arranged by size:
708 588 314 268 220 205 201 141
134 113 109 64 56 51 43 32
32
BbeI G_GCGC'C
Cuts at: 0 1264 3279
Size: 1264 2015
BbsI GAAGACnn'nnnn_
Cuts at: 0 559 899 1188 1994 3279
Size: 559 340 289 806 1285
Fragments arranged by size:
1285 806 559 340 289
BbvCI CC'TCA_GC
Cuts at: 0 1149 1771 2642 3193 3258 3279
Size: 1149 622 871 551 65 21
Fragments arranged by size:
1149 871 622 551 65 21
BglI GCCn_nnn'nGGC
Cuts at: 0 466 1507 2750 3082 3279
Size: 466 1041 1243 332 197
Fragments arranged by size:
1243 1041 466 332 197
Bme1580I G_kGCm'C
Cuts at: 0 83 205 252 267 462 1022 1214 1257
Size: 83 122 47 15 195 560 192 43
Cuts at: 1257 1468 1477 1503 1745 2279 2333 2562 2607
Size: 211 9 26 242 534 54 229 45
Cuts at: 2607 2760 3279
Size: 153 519
Fragments arranged by size:
560 534 519 242 229 211 195 192
153 122 83 54 47 45 43 26
15 9
BmgBI CAC'GTC
Cuts at: 0 450 1293 3279
Size: 450 843 1986
Fragments arranged by size:
1986 843 450
BmrI ACTGGGnnnn_n'
Cuts at: 0 1478 1490 3279
Size: 1478 12 1789
Fragments arranged by size:
1789 1478 12
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 517 614 1102 1224 1565 1648 1713 1972
Size: 517 97 488 122 341 83 65 259
Cuts at: 1972 2140 2503 2824 2833 3092 3146 3279
Size: 168 363 321 9 259 54 133
Fragments arranged by size:
517 488 363 341 321 259 259 168
133 122 97 83 65 54 9
Bpu10I CC'TnA_GC
Cuts at: 0 1149 1245 1771 2221 2642 3193 3258 3279
Size: 1149 96 526 450 421 551 65 21
Fragments arranged by size:
1149 551 526 450 421 96 65 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1641 2391 2760 3279
Size: 1641 750 369 519
Fragments arranged by size:
1641 750 519 369
BsaI GGTCTCn'nnnn_
Cuts at: 0 825 862 1363 3279
Size: 825 37 501 1916
Fragments arranged by size:
1916 825 501 37
BsaAI yAC'GTr
Cuts at: 0 255 330 3279
Size: 255 75 2949
Fragments arranged by size:
2949 255 75
BsaHI Gr'CG_yC
Cuts at: 0 1261 2147 3279
Size: 1261 886 1132
Fragments arranged by size:
1261 1132 886
BsaWI w'CCGG_w
Cuts at: 0 1967 3279
Size: 1967 1312
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 805 835 1087 1090 1117 1120 1663 1693
Size: 805 30 252 3 27 3 543 30
Cuts at: 1693 1984 2014 3065 3095 3279
Size: 291 30 1051 30 184
Fragments arranged by size:
1051 805 543 291 252 184 30 30
30 30 27 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 385 599 611 614 660 813 816 972
Size: 385 214 12 3 46 153 3 156
Cuts at: 972 984 987 996 999 1108 1431 1716 1794
Size: 12 3 9 3 109 323 285 78
Cuts at: 1794 1903 2506 3279
Size: 109 603 773
Fragments arranged by size:
773 603 385 323 285 214 156 153
109 109 78 46 12 12 9 3
3 3 3
BseYI C'CCAG_C
Cuts at: 0 47 245 778 1476 1502 1615 2579 2622
Size: 47 198 533 698 26 113 964 43
Cuts at: 2622 2956 2966 3279
Size: 334 10 313
Fragments arranged by size:
964 698 533 334 313 198 113 47
43 26 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 108 2039 2161 2284 3279
Size: 108 1931 122 123 995
Fragments arranged by size:
1931 995 123 122 108
BsiHKAI G_wGCw'C
Cuts at: 0 302 1054 2377 3279
Size: 302 752 1323 902
Fragments arranged by size:
1323 902 752 302
BsmI GAATG_Cn'
Cuts at: 0 1069 3279
Size: 1069 2210
Bsp1286I G_dGCh'C
Cuts at: 0 83 205 252 267 302 314 462 1022
Size: 83 122 47 15 35 12 148 560
Cuts at: 1022 1054 1163 1214 1257 1468 1477 1503 1745
Size: 32 109 51 43 211 9 26 242
Cuts at: 1745 2279 2333 2377 2389 2530 2562 2607 2696
Size: 534 54 44 12 141 32 45 89
Cuts at: 2696 2760 2965 3078 3279
Size: 64 205 113 201
Fragments arranged by size:
560 534 242 211 205 201 148 141
122 113 109 89 83 64 54 51
47 45 44 43 35 32 32 26
15 12 12 9
BspHI T'CATG_A
Cuts at: 0 336 3279
Size: 336 2943
BsrBI CCG'CTC
Cuts at: 0 1778 2244 3279
Size: 1778 466 1035
Fragments arranged by size:
1778 1035 466
BsrFI r'CCGG_y
Cuts at: 0 653 2143 2353 3279
Size: 653 1490 210 926
Fragments arranged by size:
1490 926 653 210
BssSI C'ACGA_G
Cuts at: 0 700 850 3279
Size: 700 150 2429
Fragments arranged by size:
2429 700 150
BstAPI GCAn_nnn'nTGC
Cuts at: 0 330 1345 2184 2946 3127 3279
Size: 330 1015 839 762 181 152
Fragments arranged by size:
1015 839 762 330 181 152
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1510 1737 1882 2637 2988 3279
Size: 1510 227 145 755 351 291
Fragments arranged by size:
1510 755 351 291 227 145
BstYI r'GATC_y
Cuts at: 0 2216 2918 3279
Size: 2216 702 361
Fragments arranged by size:
2216 702 361
Bsu36I CC'TnA_GG
Cuts at: 0 1988 3279
Size: 1988 1291
BtgI C'CryG_G
Cuts at: 0 305 426 797 1425 1892 2636 2975 3279
Size: 305 121 371 628 467 744 339 304
Fragments arranged by size:
744 628 467 371 339 305 304 121
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 89 3279
Size: 89 3190
BtsI GCAGTG_nn'
Cuts at: 0 633 806 954 1523 2676 3170 3222 3279
Size: 633 173 148 569 1153 494 52 57
Fragments arranged by size:
1153 633 569 494 173 148 57 52
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 91 126 3279
Size: 91 35 3153
Fragments arranged by size:
3153 91 35
DraIII CAC_nnn'GTG
Cuts at: 0 258 1345 2062 3279
Size: 258 1087 717 1217
Fragments arranged by size:
1217 1087 717 258
EaeI y'GGCC_r
Cuts at: 0 95 2070 2231 2978 3116 3279
Size: 95 1975 161 747 138 163
Fragments arranged by size:
1975 747 163 161 138 95
EarI CTCTTCn'nnn_
Cuts at: 0 220 712 843 990 1005 1812 2041 3279
Size: 220 492 131 147 15 807 229 1238
Fragments arranged by size:
1238 807 492 229 220 147 131 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 596 966 3170 3279
Size: 596 370 2204 109
Fragments arranged by size:
2204 596 370 109
EcoICRI GAG'CTC
Cuts at: 0 1052 3279
Size: 1052 2227
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 517 525 614 1102 1115 1224 1408 1565
Size: 517 8 89 488 13 109 184 157
Cuts at: 1565 1648 1713 1972 2140 2188 2503 2803 2824
Size: 83 65 259 168 48 315 300 21
Cuts at: 2824 2833 3092 3146 3279
Size: 9 259 54 133
Fragments arranged by size:
517 488 315 300 259 259 184 168
157 133 109 89 83 65 54 48
21 13 9 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1379 3279
Size: 1379 1900
EcoO109I rG'GnC_Cy
Cuts at: 0 139 1018 1019 1307 1473 1671 1741 1742
Size: 139 879 1 288 166 198 70 1
Cuts at: 1742 1751 1979 2330 2442 2558 2559 2676 2756
Size: 9 228 351 112 116 1 117 80
Cuts at: 2756 2757 3279
Size: 1 522
Fragments arranged by size:
879 522 351 288 228 198 166 139
117 116 112 80 70 9 1 1
1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 757 1212 3279
Size: 757 455 2067
Fragments arranged by size:
2067 757 455
HaeII r_GCGC'y
Cuts at: 0 593 1264 2176 2946 3279
Size: 593 671 912 770 333
Fragments arranged by size:
912 770 671 593 333
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 805 837 1090 1122 1267 1299 1663 1695
Size: 805 32 253 32 145 32 364 32
Cuts at: 1695 1971 1984 2003 2016 2434 2466 3149 3181
Size: 276 13 19 13 418 32 683 32
Cuts at: 3181 3279
Size: 98
Fragments arranged by size:
805 683 418 364 276 253 145 98
32 32 32 32 32 32 19 13
13
HincII GTy'rAC
Cuts at: 0 436 2151 3279
Size: 436 1715 1128
Fragments arranged by size:
1715 1128 436
HindIII A'AGCT_T
Cuts at: 0 107 440 2736 3279
Size: 107 333 2296 543
Fragments arranged by size:
2296 543 333 107
KasI G'GCGC_C
Cuts at: 0 1260 3279
Size: 1260 2019
KpnI G_GTAC'C
Cuts at: 0 3026 3279
Size: 3026 253
MluI A'CGCG_T
Cuts at: 0 83 3279
Size: 83 3196
MscI TGG'CCA
Cuts at: 0 2980 3279
Size: 2980 299
MslI CAynn'nnrTG
Cuts at: 0 1354 1831 2635 3279
Size: 1354 477 804 644
Fragments arranged by size:
1354 804 644 477
MspA1I CmG'CkG
Cuts at: 0 63 572 1480 1506 2202 2508 2617 2719
Size: 63 509 908 26 696 306 109 102
Cuts at: 2719 2772 2874 2970 3009 3070 3127 3133 3244
Size: 53 102 96 39 61 57 6 111
Cuts at: 3244 3279
Size: 35
Fragments arranged by size:
908 696 509 306 111 109 102 102
96 63 61 57 53 39 35 26
6
NaeI GCC'GGC
Cuts at: 0 2145 2355 3279
Size: 2145 210 924
Fragments arranged by size:
2145 924 210
NarI GG'CG_CC
Cuts at: 0 1261 3279
Size: 1261 2018
NcoI C'CATG_G
Cuts at: 0 305 426 1425 1892 2636 2975 3279
Size: 305 121 999 467 744 339 304
Fragments arranged by size:
999 744 467 339 305 304 121
NgoMIV G'CCGG_C
Cuts at: 0 2143 2353 3279
Size: 2143 210 926
Fragments arranged by size:
2143 926 210
PasI CC'CwG_GG
Cuts at: 0 1270 1747 2092 2326 3279
Size: 1270 477 345 234 953
Fragments arranged by size:
1270 953 477 345 234
PfoI T'CCnGG_A
Cuts at: 0 2391 3279
Size: 2391 888
PmlI CAC'GTG
Cuts at: 0 255 3279
Size: 255 3024
PpuMI rG'GwC_Cy
Cuts at: 0 139 1671 1751 1979 2442 3279
Size: 139 1532 80 228 463 837
Fragments arranged by size:
1532 837 463 228 139 80
PshAI GACnn'nnGTC
Cuts at: 0 2896 3279
Size: 2896 383
PspOMI G'GGCC_C
Cuts at: 0 1018 1210 1253 1473 1741 2329 2558 2756
Size: 1018 192 43 220 268 588 229 198
Cuts at: 2756 3279
Size: 523
Fragments arranged by size:
1018 588 523 268 229 220 198 192
43
PstI C_TGCA'G
Cuts at: 0 326 1035 1049 1714 1963 2189 2252 2771
Size: 326 709 14 665 249 226 63 519
Cuts at: 2771 2942 2951 3132 3138 3279
Size: 171 9 181 6 141
Fragments arranged by size:
709 665 519 326 249 226 181 171
141 63 14 9 6
PvuII CAG'CTG
Cuts at: 0 1480 1506 2772 2874 2970 3127 3133 3279
Size: 1480 26 1266 102 96 157 6 146
Fragments arranged by size:
1480 1266 157 146 102 96 26 6
SacI G_AGCT'C
Cuts at: 0 1054 3279
Size: 1054 2225
SalI G'TCGA_C
Cuts at: 0 434 3279
Size: 434 2845
SanDI GG'GwC_CC
Cuts at: 0 139 3279
Size: 139 3140
ScaI AGT'ACT
Cuts at: 0 517 1105 3279
Size: 517 588 2174
Fragments arranged by size:
2174 588 517
SfcI C'TryA_G
Cuts at: 0 322 1031 1045 1710 1959 2185 2248 2767
Size: 322 709 14 665 249 226 63 519
Cuts at: 2767 2938 2947 3128 3134 3279
Size: 171 9 181 6 145
Fragments arranged by size:
709 665 519 322 249 226 181 171
145 63 14 9 6
SfoI GGC'GCC
Cuts at: 0 1262 3279
Size: 1262 2017
SmaI CCC'GGG
Cuts at: 0 1258 2610 3279
Size: 1258 1352 669
Fragments arranged by size:
1352 1258 669
SmlI C'TyrA_G
Cuts at: 0 742 1620 2370 2739 3279
Size: 742 878 750 369 540
Fragments arranged by size:
878 750 742 540 369
SrfI GCCC'GGGC
Cuts at: 0 1258 3279
Size: 1258 2021
StuI AGG'CCT
Cuts at: 0 1385 3279
Size: 1385 1894
StyI C'CwwG_G
Cuts at: 0 305 426 1166 1425 1442 1892 2636 2697
Size: 305 121 740 259 17 450 744 61
Cuts at: 2697 2975 3279
Size: 278 304
Fragments arranged by size:
744 740 450 305 304 278 259 121
61 17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 709 1336 1885 3279
Size: 709 627 549 1394
Fragments arranged by size:
1394 709 627 549
TatI w'GTAC_w
Cuts at: 0 515 1103 3279
Size: 515 588 2176
Fragments arranged by size:
2176 588 515
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3065 3097 3279
Size: 3065 32 182
Fragments arranged by size:
3065 182 32
Tth111I GACn'n_nGTC
Cuts at: 0 508 817 3279
Size: 508 309 2462
Fragments arranged by size:
2462 508 309
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 112 140 1503 1623 2541 3279
Size: 112 28 1363 120 918 738
Fragments arranged by size:
1363 918 738 120 112 28
XmaI C'CCGG_G
Cuts at: 0 1256 2608 3279
Size: 1256 1352 671
Fragments arranged by size:
1352 1256 671
Enzymes that do cut:
AccI Acc65I AcuI AflII AflIII AhdI AleI AlfI
AlwNI ApaI AvaI BanI BanII BbeI BbsI BbvCI
BglI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspHI BsrBI BsrFI BssSI BstAPI BstXI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraIII EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII
Hin4I HincII HindIII KasI KpnI MluI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV PasI PfoI PmlI
PpuMI PshAI PspOMI PstI PvuII SacI SalI SanDI
ScaI SfcI SfoI SmaI SmlI SrfI StuI StyI
TaqII TatI TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII AclI AfeI AgeI AloI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BcgI BciVI
BclI BfrBI BglII BlpI BmtI BplI BsaBI BsiEI
BsiWI BsmBI BspEI BspMI BsrDI BsrGI BssHII BstBI
BstEII BstZ17I ClaI DraI DrdI EagI EcoRI EcoRV
FseI FspI FspAI HpaI MfeI MmeI NdeI NheI
NotI NruI NsiI NspI PacI PciI PflMI PmeI
PpiI PsiI PspXI PsrI PvuI RsrII SacII SapI
SbfI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI
SspI SwaI XbaI XhoI XmnI ZraI