Restriction map for
pFN21AB4275
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# NO CUT ENZYMES ( 116 enzymes) #
##################################
AatII AccII AclI AcyI AflII
AgeI AhdI Aor51HI AscI AseI
AsnI Asp700I AspEI AspI AviII
AvrII BamHI BbeI BcgI BfrI
BglII BlnI BlpI Bpu1102I BsaHI
BsaI BsiEI BsiWI BsmAI BsmBI
Bsp1407I BspDI BspHI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstUI BstXI BstZ17I CelII
CfoI Cfr10I ClaI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoRV EcoT14I FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HpaI KasI
KspI MluI MslI MvnI NaeI
NarI NcoI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI StuI
StyI SwaI Tth111I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
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Acc65I 1453 GGTACC G^GTACC
AccI 1340 GTCTAC GT^[AC][GT]AC
AccIII 371 TCCGGA T^CCGGA
AflIII 863 ACATGT A^C[GA][CT]GT
Alw44I 181 GTGCAC G^TGCAC
AocI 559 CCTGAGG CC^T[ACGT]AGG
ApaI 975 GGGCCC GGGCC^C
ApaLI 181 GTGCAC G^TGCAC
Asp718I 1453 GGTACC G^GTACC
BalI 1088 TGGCCA TGG^CCA
BbrPI 1435 CACGTG CAC^GTG
BbsI 455 GAAGAC GAAGAC(2/6)
BciVI 1621 GGATAC GTATCC(6/5)
BclI 1715 TGATCA T^GATCA
BfaI 13 CTAG C^TAG
BglI 88 GCCTCCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 455 GAAGAC GAAGAC(2/6)
BsaAI 1435 CACGTG [CT]AC^GT[GA]
BsaBI 469 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 371 TCCGGA [AT]^CCGG[AT]
BseAI 371 TCCGGA T^CCGGA
BsgI 1006 CTGCAC GTGCAG(16/14)
BsmI 312 GAATGC GAATGC(1/-1)
BspEI 371 TCCGGA T^CCGGA
BspLU11I 863 ACATGT A^CATGT
BsrDI 1386 CATTGC GCAATG(2/0)
BstEII 134 GGTCACC G^GT[ACGT]ACC
BstPI 134 GGTCACC G^GT[ACGT]ACC
Bsu36I 559 CCTGAGG CC^T[ACGT]AGG
DsaI 517 CCACGG C^C[GA][CT]GG
EarI 798 CTCTTC CTCTTC(1/4)
Eco81I 559 CCTGAGG CC^T[ACGT]AGG
EcoO65I 134 GGTCACC G^GT[ACGT]ACC
EcoRI 333 GAATTC G^AATTC
EcoT22I 1093 ATGCAT ATGCA^T
FauI 1104 CCCGC CCCGC(4/6)
FbaI 1715 TGATCA T^GATCA
KpnI 1453 GGTACC GGTAC^C
Ksp632I 798 CTCTTC CTCTTC(1/4)
MaeI 13 CTAG C^TAG
MaeII 1436 ACGT A^CGT
MamI 469 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1088 TGGCCA TGG^CCA
MroI 371 TCCGGA T^CCGGA
MscI 1088 TGGCCA TGG^CCA
NdeI 1642 CATATG CA^TATG
NsiI 1093 ATGCAT ATGCA^T
NspI 863 ACATGT [GA]CATG^[CT]
PmaCI 1435 CACGTG CAC^GTG
PmlI 1435 CACGTG CAC^GTG
PpuMI 681 GGGTCCC [GA]G^G[AT]CC[CT]
PstI 1108 CTGCAG CTGCA^G
SmaI 106 CCCGGG CCC^GGG
SmlI 1445 CTCAAG C^T[CT][GA]AG
SspI 1249 AATATT AAT^ATT
XcmI 1566 CCACCTCTACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 106 CCCGGG C^CCGGG
XmaI 106 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcsI 333 GAATTC [GA]^AATT[CT]
718 AAATTC
AlwNI 623 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG
1469 CAGCCCCTG
ApoI 333 GAATTC [GA]^AATT[CT]
718 AAATTC
AvaI 106 CCCGGG C^[CT]CG[GA]G
389 CTCGGG
BcnI 106 CCCGG CC^[GC]GG
107 CCGGG
BsoBI 106 CCCGGG C^[CT]CG[GA]G
389 CTCGGG
BspMI 430 ACCTGC ACCTGC(4/8)
966 ACCTGC
DraI 715 TTTAAA TTT^AAA
1260 TTTAAA
EaeI 1088 TGGCCA [CT]^GGCC[GA]
1654 CGGCCA
HapII 107 CCGG C^CGG
372 CCGG
HindIII 76 AAGCTT A^AGCTT
1269 AAGCTT
HpaII 107 CCGG C^CGG
372 CCGG
MfeI 422 CAATTG C^AATTG
1293 CAATTG
MseI 716 TTAA T^TAA
1261 TTAA
MspI 107 CCGG C^CGG
372 CCGG
MunI 422 CAATTG C^AATTG
1293 CAATTG
NciI 106 CCCGG CC^[GC]GG
107 CCGGG
PflMI 32 CCATAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1666 CCACCTCCTGG
PvuII 49 CAGCTG CAG^CTG
983 CAGCTG
SfaNI 1095 GCATC GCATC(5/9)
1380 GCATC
TfiI 567 GAATC G^A[AT]TC
1284 GAATC
Tru9I 716 TTAA T^TAA
1261 TTAA
Van91I 32 CCATAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1666 CCACCTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BanI BanII BbvCI BbvI
BmyI BpmI BsaJI BseRI BsiHKAI
BsiYI BslI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraII EcoO109I
EcoRII Fnu4HI FokI HaeIII HinfI
HphI ItaI MaeIII MboI MboII
MflI MnlI MspA1I MvaI MwoI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfcI TaqI
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII