(Circular) (Six-base) MAPSORT of: pFN21AB4275.seq Check: 5934 from: 1 to: 6427 pFN21AB4275 6427 bp With 182 enzymes: SgfI * February 27, 2009 14:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2444 2980 2444 Size: 536 5891 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4237 278 Size: 53 83 186 936 2701 2468 Fragments arranged by size: 2701 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3346 3770 1937 Size: 1409 424 4594 Fragments arranged by size: 4594 1409 424 Acc65I G'GTAC_C Cuts at: 3458 3748 3458 Size: 290 6137 AclI AA'CG_TT Cuts at: 1563 4554 4927 1563 Size: 2991 373 3063 Fragments arranged by size: 3063 2991 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2573 2649 4490 5874 6142 1632 Size: 941 76 1841 1384 268 1917 Fragments arranged by size: 1917 1841 1384 941 268 76 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6230 AflIII A'CryG_T Cuts at: 1203 2868 5326 6303 1203 Size: 1665 2458 977 1327 Fragments arranged by size: 2458 1665 1327 977 AgeI A'CCGG_T Cuts at: 5258 5258 Size: 6427 AhdI GACnn_n'nnGTC Cuts at: 5156 5156 Size: 6427 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6427 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2988 3408 1184 Size: 1804 420 4203 Fragments arranged by size: 4203 1804 420 AlwNI CAG_nnn'CTG Cuts at: 2633 3479 4170 5742 2633 Size: 846 691 1572 3318 Fragments arranged by size: 3318 1572 846 691 ApaI G_GGCC'C Cuts at: 2984 2984 Size: 6427 ApaLI G'TGCA_C Cuts at: 2186 4484 5640 2186 Size: 2298 1156 2973 Fragments arranged by size: 2973 2298 1156 ApoI r'AATT_y Cuts at: 1493 2338 2723 3736 3962 4114 6223 1493 Size: 845 385 1013 226 152 2109 1697 Fragments arranged by size: 2109 1697 1013 845 385 226 152 AseI AT'TA_AT Cuts at: 160 1032 4981 160 Size: 872 3949 1606 Fragments arranged by size: 3949 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6427 AvaI C'yCGr_G Cuts at: 1870 1943 2111 2394 3752 1870 Size: 73 168 283 1358 4545 Fragments arranged by size: 4545 1358 283 168 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3581 3614 3581 Size: 33 6394 BamHI G'GATC_C Cuts at: 3757 4124 3757 Size: 367 6060 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2383 2968 3458 3555 3680 Size: 325 217 627 596 585 490 97 125 Cuts at: 3680 3748 5203 618 Size: 68 1455 1842 Fragments arranged by size: 1842 1455 627 596 585 490 325 217 125 97 68 BanII G_rGCy'C Cuts at: 1391 2522 2597 2984 3746 1391 Size: 1131 75 387 762 4072 Fragments arranged by size: 4072 1131 762 387 75 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2467 928 Size: 971 568 4888 Fragments arranged by size: 4888 971 568 BbvCI CC'TCA_GC Cuts at: 2270 2682 2986 2270 Size: 412 304 5711 Fragments arranged by size: 5711 412 304 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4618 4652 1903 Size: 34 2681 34 3678 Fragments arranged by size: 3678 2681 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3620 4319 5529 3620 Size: 699 1210 4518 Fragments arranged by size: 4518 1210 699 BclI T'GATC_A Cuts at: 1548 3720 1548 Size: 2172 4255 BfrBI ATG'CAT Cuts at: 3100 3100 Size: 6427 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2099 5038 136 Size: 107 122 71 631 605 427 2939 1525 Fragments arranged by size: 2939 1525 631 605 427 122 107 71 BglII A'GATC_T Cuts at: 6422 6422 Size: 6427 BlpI GC'TnA_GC Cuts at: 3844 3844 Size: 6427 Bme1580I G_kGCm'C Cuts at: 1790 2190 2388 2627 2984 3447 3505 3558 4488 Size: 400 198 239 357 463 58 53 930 Cuts at: 4488 5644 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 930 463 400 357 239 198 58 53 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6427 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3721 4158 5116 Size: 416 53 83 399 24 2304 437 958 Cuts at: 5116 442 Size: 1753 Fragments arranged by size: 2304 1753 958 437 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6427 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2818 2818 Size: 6427 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2235 2454 2813 5087 1239 Size: 996 219 359 2274 2579 Fragments arranged by size: 2579 2274 996 359 219 Bpu10I CC'TnA_GC Cuts at: 2165 2270 2682 2986 2165 Size: 105 412 304 5606 Fragments arranged by size: 5606 412 304 105 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3435 4552 5417 5715 3435 Size: 1117 865 298 4147 Fragments arranged by size: 4147 1117 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5090 882 Size: 617 3591 2219 Fragments arranged by size: 3591 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3442 4218 493 Size: 711 2238 776 2702 Fragments arranged by size: 2702 2238 776 711 BsaBI GATnn'nnATC Cuts at: 2478 6421 2478 Size: 3943 2484 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4234 4616 5251 275 Size: 53 83 186 936 2701 382 635 1451 Fragments arranged by size: 2701 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2376 4860 5258 5532 5679 6090 2376 Size: 2484 398 274 147 411 2713 Fragments arranged by size: 2713 2484 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6202 Fragments arranged by size: 6202 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2236 2800 2994 3704 1186 Size: 1050 564 194 710 3909 Fragments arranged by size: 3909 1050 710 564 194 BseYI C'CCAG_C Cuts at: 2052 2102 2940 2949 3059 3165 3637 5630 6126 Size: 50 838 9 110 106 472 1993 496 Cuts at: 6126 2052 Size: 2353 Fragments arranged by size: 2353 1993 838 496 472 110 106 50 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2996 1163 Size: 573 1260 4594 Fragments arranged by size: 4594 1260 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3877 4638 5666 1540 Size: 275 186 1876 761 1028 2301 Fragments arranged by size: 2301 1876 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2190 3004 3251 4488 4573 5644 2190 Size: 814 247 1237 85 1071 2973 Fragments arranged by size: 2973 1237 1071 814 247 85 BsmI GAATG_Cn' Cuts at: 2323 3938 4031 2323 Size: 1615 93 4719 Fragments arranged by size: 4719 1615 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2190 2388 2522 2597 2627 2984 3004 Size: 399 400 198 134 75 30 357 20 Cuts at: 3004 3251 3447 3505 3558 3746 4488 4573 5644 Size: 247 196 58 53 188 742 85 1071 Cuts at: 5644 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 400 399 357 247 198 196 188 134 85 75 58 53 30 20 BspEI T'CCGG_A Cuts at: 2376 6090 2376 Size: 3714 2713 BspHI T'CATG_A Cuts at: 4316 6046 4316 Size: 1730 4697 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2444 2980 3782 844 Size: 1600 536 802 3489 Fragments arranged by size: 3489 1600 802 536 BsrBI CCG'CTC Cuts at: 1394 1823 4314 1394 Size: 429 2491 3507 Fragments arranged by size: 3507 2491 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3390 4922 5096 65 Size: 1183 2142 1532 174 1396 Fragments arranged by size: 2142 1532 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5071 5258 1700 Size: 110 3261 187 2869 Fragments arranged by size: 3261 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6427 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6427 BssSI C'ACGA_G Cuts at: 4487 5499 4487 Size: 1012 5415 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6427 BstBI TT'CG_AA Cuts at: 5231 5231 Size: 6427 BstEII G'GTnAC_C Cuts at: 1184 2139 1184 Size: 955 5472 BstYI r'GATC_y Cuts at: 1972 2149 2289 2630 2752 3693 3757 4124 4509 Size: 177 140 341 122 941 64 367 385 Cuts at: 4509 4526 5978 6064 6076 6422 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 941 385 367 346 341 177 140 122 86 64 17 12 Bsu36I CC'TnA_GG Cuts at: 2565 2565 Size: 6427 BtgI C'CryG_G Cuts at: 513 1065 2004 2522 513 Size: 552 939 518 4418 Fragments arranged by size: 4418 939 552 518 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5688 Fragments arranged by size: 5688 683 56 BtsI GCAGTG_nn' Cuts at: 2190 2555 2655 3008 3943 4737 4757 2190 Size: 365 100 353 935 794 20 3860 Fragments arranged by size: 3860 935 794 365 353 100 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6427 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6392 DraI TTT'AAA Cuts at: 2722 3267 3731 2722 Size: 545 464 5418 Fragments arranged by size: 5418 545 464 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5434 809 Size: 507 4118 1802 Fragments arranged by size: 4118 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3093 3659 3874 Size: 54 1425 50 275 18 1263 566 215 Cuts at: 3874 4763 6392 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1263 889 566 275 215 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3874 1537 Size: 275 2062 4090 Fragments arranged by size: 4090 2062 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2809 4357 1353 Size: 1456 1548 3423 Fragments arranged by size: 3423 1548 1456 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3080 5014 5388 5534 387 Size: 1064 138 1491 1934 374 146 1280 Fragments arranged by size: 1934 1491 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2235 2454 2573 2649 2813 4490 5087 Size: 393 603 219 119 76 164 1677 597 Cuts at: 5087 5874 6142 1239 Size: 787 268 1524 Fragments arranged by size: 1677 1524 787 603 597 393 268 219 164 119 76 EcoO109I rG'GnC_Cy Cuts at: 2687 2980 2981 2687 Size: 293 1 6133 Fragments arranged by size: 6133 293 1 EcoRI G'AATT_C Cuts at: 2338 3736 4114 2338 Size: 1398 378 4651 Fragments arranged by size: 4651 1398 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3292 3292 Size: 6427 FspI TGC'GCA Cuts at: 4933 4933 Size: 6427 HaeII r_GCGC'y Cuts at: 1678 5574 1678 Size: 3896 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2468 2500 3233 3265 3274 Size: 32 135 32 896 32 733 32 9 Cuts at: 3274 3306 3328 3360 5068 5100 5142 5174 1373 Size: 32 22 32 1708 32 42 32 2626 Fragments arranged by size: 2626 1708 896 733 135 42 32 32 32 32 32 32 32 32 22 9 HincII GTy'rAC Cuts at: 669 1657 1938 3771 4017 669 Size: 988 281 1833 246 3079 Fragments arranged by size: 3079 1833 988 281 246 HindIII A'AGCT_T Cuts at: 748 2081 3274 748 Size: 1333 1193 3901 Fragments arranged by size: 3901 1333 1193 HpaI GTT'AAC Cuts at: 4017 4017 Size: 6427 KpnI G_GTAC'C Cuts at: 3462 3752 3462 Size: 290 6137 MfeI C'AATT_G Cuts at: 2427 3298 4026 2427 Size: 871 728 4828 Fragments arranged by size: 4828 871 728 MluI A'CGCG_T Cuts at: 6303 6303 Size: 6427 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2312 5541 5725 1305 Size: 1007 3229 184 2007 Fragments arranged by size: 3229 2007 1007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3095 10 Size: 54 1425 343 1263 3342 Fragments arranged by size: 3342 1425 1263 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4385 4744 4903 518 Size: 648 3219 359 159 2042 Fragments arranged by size: 3219 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2056 2286 2990 3112 3843 4520 5668 5913 Size: 450 230 704 122 731 677 1148 245 Cuts at: 5913 1606 Size: 2120 Fragments arranged by size: 2120 1148 731 704 677 450 245 230 122 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4936 Fragments arranged by size: 4936 939 552 NdeI CA'TA_TG Cuts at: 387 3648 387 Size: 3261 3166 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6427 NotI GC'GGCC_GC Cuts at: 3874 3874 Size: 6427 NsiI A_TGCA'T Cuts at: 3102 3102 Size: 6427 NspI r_CATG'y Cuts at: 1132 1229 2872 3785 5330 1132 Size: 97 1643 913 1545 2229 Fragments arranged by size: 2229 1643 1545 913 97 PasI CC'CwG_GG Cuts at: 1348 2405 2977 1348 Size: 1057 572 4798 Fragments arranged by size: 4798 1057 572 PciI A'CATG_T Cuts at: 2868 5326 2868 Size: 2458 3969 PflMI CCAn_nnn'nTGG Cuts at: 1108 2043 3677 1108 Size: 935 1634 3858 Fragments arranged by size: 3858 1634 935 PmeI GTTT'AAAC Cuts at: 3731 3731 Size: 6427 PmlI CAC'GTG Cuts at: 3442 3442 Size: 6427 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4496 4528 6009 6041 4496 Size: 32 1481 32 4882 Fragments arranged by size: 4882 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2687 2687 Size: 6427 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6427 PsiI TTA'TAA Cuts at: 3997 3997 Size: 6427 PspOMI G'GGCC_C Cuts at: 2980 2980 Size: 6427 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2849 2881 2849 Size: 32 6395 PstI C_TGCA'G Cuts at: 830 3117 3779 830 Size: 2287 662 3478 Fragments arranged by size: 3478 2287 662 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6427 PvuII CAG'CTG Cuts at: 2056 2990 2056 Size: 934 5493 SalI G'TCGA_C Cuts at: 1936 3769 1936 Size: 1833 4594 SanDI GG'GwC_CC Cuts at: 2687 2687 Size: 6427 SbfI CC_TGCA'GG Cuts at: 3779 3779 Size: 6427 ScaI AGT'ACT Cuts at: 4675 6387 4675 Size: 1712 4715 SfcI C'TryA_G Cuts at: 826 1045 2604 2614 2991 3113 3775 4910 5591 Size: 219 1559 10 377 122 662 1135 681 Cuts at: 5591 5782 826 Size: 191 1471 Fragments arranged by size: 1559 1471 1135 681 662 377 219 191 122 10 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6427 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6427 SmaI CCC'GGG Cuts at: 1872 2113 3754 1872 Size: 241 1641 4545 Fragments arranged by size: 4545 1641 241 SmlI C'TyrA_G Cuts at: 820 1017 1943 3450 4531 5432 5694 820 Size: 197 926 1507 1081 901 262 1553 Fragments arranged by size: 1553 1507 1081 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4218 493 Size: 3725 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6427 SphI G_CATG'C Cuts at: 1132 3785 1132 Size: 2653 3774 SspI AAT'ATT Cuts at: 5 52 3256 4351 5 Size: 47 3204 1095 2081 Fragments arranged by size: 3204 2081 1095 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6427 StyI C'CwwG_G Cuts at: 513 1065 2004 3866 513 Size: 552 939 1862 3074 Fragments arranged by size: 3074 1862 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2158 3573 3626 4454 4471 4624 4809 Size: 1255 578 1415 53 828 17 153 185 Cuts at: 4809 5254 325 Size: 445 1498 Fragments arranged by size: 1498 1415 1255 828 578 445 185 153 53 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4673 6385 96 Size: 274 80 33 51 1443 2696 1712 138 Fragments arranged by size: 2696 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6387 6419 6387 Size: 32 6395 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6427 XbaI T'CTAG_A Cuts at: 3763 3763 Size: 6427 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3578 3578 Size: 6427 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6427 XmaI C'CCGG_G Cuts at: 1870 2111 3752 1870 Size: 241 1641 4545 Fragments arranged by size: 4545 1641 241 XmnI GAAnn'nnTTC Cuts at: 4556 4556 Size: 6427 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4235 276 Size: 53 83 186 936 2701 2468 Fragments arranged by size: 2701 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbeI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PspXI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI