Restriction map for
pFN21AB4275
(Linear) (Six-base) MAPSORT of: KIBB4275.seq Check: 9536 from: 1 to: 1722
KIBB4275.orf
With 182 enzymes: SgfI *
July 18, 2007 13:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 439 975 1722
Size: 439 536 747
Fragments arranged by size:
747 536 439
AccI GT'mk_AC
Cuts at: 0 1341 1722
Size: 1341 381
Acc65I G'GTAC_C
Cuts at: 0 1453 1722
Size: 1453 269
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 568 644 1722
Size: 568 76 1078
Fragments arranged by size:
1078 568 76
AflIII A'CryG_T
Cuts at: 0 863 1722
Size: 863 859
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 983 1403 1722
Size: 983 420 319
Fragments arranged by size:
983 420 319
AlwNI CAG_nnn'CTG
Cuts at: 0 628 1474 1722
Size: 628 846 248
Fragments arranged by size:
846 628 248
ApaI G_GGCC'C
Cuts at: 0 979 1722
Size: 979 743
ApaLI G'TGCA_C
Cuts at: 0 181 1722
Size: 181 1541
ApoI r'AATT_y
Cuts at: 0 333 718 1722
Size: 333 385 1004
Fragments arranged by size:
1004 385 333
AvaI C'yCGr_G
Cuts at: 0 106 389 1722
Size: 106 283 1333
Fragments arranged by size:
1333 283 106
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1576 1609 1722
Size: 1576 33 113
Fragments arranged by size:
1576 113 33
BanI G'GyrC_C
Cuts at: 0 378 963 1453 1550 1675 1722
Size: 378 585 490 97 125 47
Fragments arranged by size:
585 490 378 125 97 47
BanII G_rGCy'C
Cuts at: 0 517 592 979 1722
Size: 517 75 387 743
Fragments arranged by size:
743 517 387 75
BbsI GAAGACnn'nnnn_
Cuts at: 0 462 1722
Size: 462 1260
BbvCI CC'TCA_GC
Cuts at: 0 265 677 981 1722
Size: 265 412 304 741
Fragments arranged by size:
741 412 304 265
BciVI GTATCCnnnnn_n'
Cuts at: 0 1615 1722
Size: 1615 107
BclI T'GATC_A
Cuts at: 0 1715 1722
Size: 1715 7
BfrBI ATG'CAT
Cuts at: 0 1095 1722
Size: 1095 627
BglI GCCn_nnn'nGGC
Cuts at: 0 94 1722
Size: 94 1628
Bme1580I G_kGCm'C
Cuts at: 0 185 383 622 979 1442 1500 1553 1722
Size: 185 198 239 357 463 58 53 169
Fragments arranged by size:
463 357 239 198 185 169 58 53
BmrI ACTGGGnnnn_n'
Cuts at: 0 1716 1722
Size: 1716 6
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 813 1722
Size: 813 909
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 230 449 808 1722
Size: 230 219 359 914
Fragments arranged by size:
914 359 230 219
Bpu10I CC'TnA_GC
Cuts at: 0 160 265 677 981 1722
Size: 160 105 412 304 741
Fragments arranged by size:
741 412 304 160 105
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1430 1722
Size: 1430 292
BsaAI yAC'GTr
Cuts at: 0 1437 1722
Size: 1437 285
BsaBI GATnn'nnATC
Cuts at: 0 473 1722
Size: 473 1249
BsaWI w'CCGG_w
Cuts at: 0 371 1722
Size: 371 1351
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 231 795 989 1699 1722
Size: 231 564 194 710 23
Fragments arranged by size:
710 564 231 194 23
BseYI C'CCAG_C
Cuts at: 0 47 97 935 944 1054 1160 1632 1722
Size: 47 50 838 9 110 106 472 90
Fragments arranged by size:
838 472 110 106 90 50 47 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 991 1722
Size: 991 731
BsiHKAI G_wGCw'C
Cuts at: 0 185 999 1246 1722
Size: 185 814 247 476
Fragments arranged by size:
814 476 247 185
BsmI GAATG_Cn'
Cuts at: 0 318 1722
Size: 318 1404
Bsp1286I G_dGCh'C
Cuts at: 0 185 383 517 592 622 979 999 1246
Size: 185 198 134 75 30 357 20 247
Cuts at: 1246 1442 1500 1553 1722
Size: 196 58 53 169
Fragments arranged by size:
357 247 198 196 185 169 134 75
58 53 30 20
BspEI T'CCGG_A
Cuts at: 0 371 1722
Size: 371 1351
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 439 975 1722
Size: 439 536 747
Fragments arranged by size:
747 536 439
BsrDI GCAATG_nn'
Cuts at: 0 1385 1722
Size: 1385 337
BstEII G'GTnAC_C
Cuts at: 0 134 1722
Size: 134 1588
BstYI r'GATC_y
Cuts at: 0 144 284 625 747 1688 1722
Size: 144 140 341 122 941 34
Fragments arranged by size:
941 341 144 140 122 34
Bsu36I CC'TnA_GG
Cuts at: 0 560 1722
Size: 560 1162
BtgI C'CryG_G
Cuts at: 0 517 1722
Size: 517 1205
BtsI GCAGTG_nn'
Cuts at: 0 185 550 650 1003 1722
Size: 185 365 100 353 719
Fragments arranged by size:
719 365 353 185 100
DraI TTT'AAA
Cuts at: 0 717 1262 1722
Size: 717 545 460
Fragments arranged by size:
717 545 460
EaeI y'GGCC_r
Cuts at: 0 1088 1654 1722
Size: 1088 566 68
Fragments arranged by size:
1088 566 68
EarI CTCTTCn'nnn_
Cuts at: 0 804 1722
Size: 804 918
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1075 1722
Size: 1075 647
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 230 449 568 644 808 1722
Size: 230 219 119 76 164 914
Fragments arranged by size:
914 230 219 164 119 76
EcoO109I rG'GnC_Cy
Cuts at: 0 682 975 976 1722
Size: 682 293 1 746
Fragments arranged by size:
746 682 293 1
EcoRI G'AATT_C
Cuts at: 0 333 1722
Size: 333 1389
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1287 1722
Size: 1287 435
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 463 495 1228 1260 1269 1301 1323 1355
Size: 463 32 733 32 9 32 22 32
Cuts at: 1355 1722
Size: 367
Fragments arranged by size:
733 463 367 32 32 32 32 22
9
HindIII A'AGCT_T
Cuts at: 0 76 1269 1722
Size: 76 1193 453
Fragments arranged by size:
1193 453 76
KpnI G_GTAC'C
Cuts at: 0 1457 1722
Size: 1457 265
MfeI C'AATT_G
Cuts at: 0 422 1293 1722
Size: 422 871 429
Fragments arranged by size:
871 429 422
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 1722
Size: 307 1415
MscI TGG'CCA
Cuts at: 0 1090 1722
Size: 1090 632
MspA1I CmG'CkG
Cuts at: 0 51 281 985 1107 1722
Size: 51 230 704 122 615
Fragments arranged by size:
704 615 230 122 51
NdeI CA'TA_TG
Cuts at: 0 1643 1722
Size: 1643 79
NsiI A_TGCA'T
Cuts at: 0 1097 1722
Size: 1097 625
NspI r_CATG'y
Cuts at: 0 867 1722
Size: 867 855
PasI CC'CwG_GG
Cuts at: 0 400 972 1722
Size: 400 572 750
Fragments arranged by size:
750 572 400
PciI A'CATG_T
Cuts at: 0 863 1722
Size: 863 859
PflMI CCAn_nnn'nTGG
Cuts at: 0 38 1672 1722
Size: 38 1634 50
Fragments arranged by size:
1634 50 38
PmlI CAC'GTG
Cuts at: 0 1437 1722
Size: 1437 285
PpuMI rG'GwC_Cy
Cuts at: 0 682 1722
Size: 682 1040
PspOMI G'GGCC_C
Cuts at: 0 975 1722
Size: 975 747
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 844 876 1722
Size: 844 32 846
Fragments arranged by size:
846 844 32
PstI C_TGCA'G
Cuts at: 0 1112 1722
Size: 1112 610
PvuII CAG'CTG
Cuts at: 0 51 985 1722
Size: 51 934 737
Fragments arranged by size:
934 737 51
SanDI GG'GwC_CC
Cuts at: 0 682 1722
Size: 682 1040
SfcI C'TryA_G
Cuts at: 0 599 609 986 1108 1722
Size: 599 10 377 122 614
Fragments arranged by size:
614 599 377 122 10
SmaI CCC'GGG
Cuts at: 0 108 1722
Size: 108 1614
SmlI C'TyrA_G
Cuts at: 0 1445 1722
Size: 1445 277
SspI AAT'ATT
Cuts at: 0 1251 1722
Size: 1251 471
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 153 1568 1621 1722
Size: 153 1415 53 101
Fragments arranged by size:
1415 153 101 53
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1573 1722
Size: 1573 149
XmaI C'CCGG_G
Cuts at: 0 106 1722
Size: 106 1616
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AlfI AlwNI ApaI
ApaLI ApoI AvaI BaeI BanI BanII BbsI BbvCI
BciVI BclI BfrBI BglI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaAI BsaBI BsaWI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspEI BspMI BsrDI BstEII BstYI
Bsu36I BtgI BtsI DraI EaeI EarI EciI Eco57MI
EcoO109I EcoRI FalI Hin4I HindIII KpnI MfeI MmeI
MscI MspA1I NdeI NsiI NspI PasI PciI PflMI
PmlI PpuMI PspOMI PsrI PstI PvuII SanDI SfcI
SmaI SmlI SspI TaqII XcmI XmaI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AhdI AleI AloI
AscI AseI AsiSI AvrII BamHI BbeI BcgI BglII
BlpI BmgBI BmtI BsaI BsaHI BsaXI BsiEI BsiWI
BsmBI BspHI BsrBI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstXI BstZ17I BtgZI ClaI CspCI DraIII DrdI
EagI EcoICRI EcoNI EcoRV FseI FspI FspAI HaeII
HincII HpaI KasI MluI MslI NaeI NarI NcoI
NgoMIV NheI NotI NruI PacI PfoI PmeI PpiI
PshAI PsiI PspXI PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI StuI StyI SwaI TatI
TstI Tth111I XbaI XhoI XmnI ZraI