(Circular) (Six-base) MAPSORT of: pFN21AB4249.seq Check: 9337 from: 1 to: 5791 pFN21AB4249 5791 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2870 2870 Size: 5791 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3601 278 Size: 53 83 186 936 2065 2468 Fragments arranged by size: 2468 2065 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3134 1937 Size: 1197 4594 Acc65I G'GTAC_C Cuts at: 3112 3112 Size: 5791 AclI AA'CG_TT Cuts at: 1563 3918 4291 1563 Size: 2355 373 3063 Fragments arranged by size: 3063 2355 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2573 3854 5238 5506 1632 Size: 941 1281 1384 268 1917 Fragments arranged by size: 1917 1384 1281 941 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5594 AflIII A'CryG_T Cuts at: 1203 4690 5667 1203 Size: 3487 977 1327 Fragments arranged by size: 3487 1327 977 AgeI A'CCGG_T Cuts at: 4622 4622 Size: 5791 AhdI GACnn_n'nnGTC Cuts at: 4520 4520 Size: 5791 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5791 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5791 AlwNI CAG_nnn'CTG Cuts at: 2079 2911 3534 5106 2079 Size: 832 623 1572 2764 Fragments arranged by size: 2764 1572 832 623 ApaLI G'TGCA_C Cuts at: 2921 3848 5004 2921 Size: 927 1156 3708 Fragments arranged by size: 3708 1156 927 ApoI r'AATT_y Cuts at: 1493 3100 3326 3478 5587 1493 Size: 1607 226 152 2109 1697 Fragments arranged by size: 2109 1697 1607 226 152 AseI AT'TA_AT Cuts at: 160 1032 4345 160 Size: 872 3313 1606 Fragments arranged by size: 3313 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5791 AvaI C'yCGr_G Cuts at: 1870 1943 2606 3116 1870 Size: 73 663 510 4545 Fragments arranged by size: 4545 663 510 73 BamHI G'GATC_C Cuts at: 3121 3488 3121 Size: 367 5424 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3112 4567 618 Size: 325 217 627 1325 1455 1842 Fragments arranged by size: 1842 1455 1325 627 325 217 BanII G_rGCy'C Cuts at: 1391 2349 3024 3110 1391 Size: 958 675 86 4072 Fragments arranged by size: 4072 958 675 86 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2561 2686 2776 928 Size: 971 662 125 90 3943 Fragments arranged by size: 3943 971 662 125 90 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3982 4016 1903 Size: 34 2045 34 3678 Fragments arranged by size: 3678 2045 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3683 4893 3683 Size: 1210 4581 BclI T'GATC_A Cuts at: 1548 1548 Size: 5791 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2723 4402 136 Size: 107 122 71 631 605 1051 1679 1525 Fragments arranged by size: 1679 1525 1051 631 605 122 107 71 BglII A'GATC_T Cuts at: 5786 5786 Size: 5791 BlpI GC'TnA_GC Cuts at: 3208 3208 Size: 5791 Bme1580I G_kGCm'C Cuts at: 1790 2925 3852 5008 1790 Size: 1135 927 1156 2573 Fragments arranged by size: 2573 1156 1135 927 BmgBI CAC'GTC Cuts at: 1318 2565 1318 Size: 1247 4544 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2291 3522 4480 Size: 416 53 83 399 24 874 1231 958 Cuts at: 4480 442 Size: 1753 Fragments arranged by size: 1753 1231 958 874 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5791 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2665 2665 Size: 5791 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2891 3028 4451 1239 Size: 1652 137 1423 2579 Fragments arranged by size: 2579 1652 1423 137 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3916 4781 5079 3916 Size: 865 298 4628 Fragments arranged by size: 4628 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2576 2653 4454 882 Size: 617 1077 77 1801 2219 Fragments arranged by size: 2219 1801 1077 617 77 BsaAI yAC'GTr Cuts at: 493 1204 2352 2412 2776 2812 3582 493 Size: 711 1148 60 364 36 770 2702 Fragments arranged by size: 2702 1148 770 711 364 60 36 BsaBI GATnn'nnATC Cuts at: 2013 5785 2013 Size: 3772 2019 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2726 3598 3980 4615 Size: 53 83 186 936 1193 872 382 635 Cuts at: 4615 275 Size: 1451 Fragments arranged by size: 1451 1193 936 872 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4224 4622 4896 5043 5454 4224 Size: 398 274 147 411 4561 Fragments arranged by size: 4561 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2129 2159 2819 2849 1178 Size: 30 165 30 726 30 660 30 4120 Fragments arranged by size: 4120 726 660 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5791 BseYI C'CCAG_C Cuts at: 2462 4994 5490 2462 Size: 2532 496 2763 Fragments arranged by size: 2763 2532 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2339 2828 1163 Size: 573 603 489 4126 Fragments arranged by size: 4126 603 573 489 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3241 4002 5030 1540 Size: 275 186 1240 761 1028 2301 Fragments arranged by size: 2301 1240 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2349 2613 2877 2925 3086 3852 3937 5008 2349 Size: 264 264 48 161 766 85 1071 3132 Fragments arranged by size: 3132 1071 766 264 264 161 85 48 BsmI GAATG_Cn' Cuts at: 3302 3395 3302 Size: 93 5698 BsmBI CGTCTCn'nnnn_ Cuts at: 3050 3050 Size: 5791 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2349 2613 2877 2925 3024 3086 3110 Size: 399 559 264 264 48 99 62 24 Cuts at: 3110 3852 3937 5008 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 559 399 264 264 99 85 62 48 24 BspEI T'CCGG_A Cuts at: 5454 5454 Size: 5791 BspHI T'CATG_A Cuts at: 3680 5410 3680 Size: 1730 4061 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2870 3146 844 Size: 2026 276 3489 Fragments arranged by size: 3489 2026 276 BsrBI CCG'CTC Cuts at: 1394 1823 3678 1394 Size: 429 1855 3507 Fragments arranged by size: 3507 1855 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2265 4286 4460 65 Size: 1183 1017 2021 174 1396 Fragments arranged by size: 2021 1396 1183 1017 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2592 2865 4435 4622 1700 Size: 110 782 273 1570 187 2869 Fragments arranged by size: 2869 1570 782 273 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5791 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5791 BssSI C'ACGA_G Cuts at: 2315 3851 4863 2315 Size: 1536 1012 3243 Fragments arranged by size: 3243 1536 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2079 794 Size: 1285 4506 BstBI TT'CG_AA Cuts at: 4595 4595 Size: 5791 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5791 BstXI CCAn_nnnn'nTGG Cuts at: 2194 2194 Size: 5791 BstYI r'GATC_y Cuts at: 1972 2008 3121 3488 3873 3890 5342 5428 5440 Size: 36 1113 367 385 17 1452 86 12 Cuts at: 5440 5786 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1113 385 367 346 86 36 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2193 2337 513 Size: 552 939 189 144 3967 Fragments arranged by size: 3967 939 552 189 144 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5052 Fragments arranged by size: 5052 683 56 BtsI GCAGTG_nn' Cuts at: 2242 2923 3307 4101 4121 2242 Size: 681 384 794 20 3912 Fragments arranged by size: 3912 794 681 384 20 ClaI AT'CG_AT Cuts at: 1554 3036 1554 Size: 1482 4309 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5756 DraI TTT'AAA Cuts at: 3095 3095 Size: 5791 DraIII CAC_nnn'GTG Cuts at: 2907 2907 Size: 5791 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2563 4798 809 Size: 507 1247 2235 1802 Fragments arranged by size: 2235 1802 1247 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2060 2262 2709 Size: 54 1425 50 275 18 230 202 447 Cuts at: 2709 3009 3238 4127 5756 8 Size: 300 229 889 1629 43 Fragments arranged by size: 1629 1425 889 447 300 275 230 229 202 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3238 1537 Size: 275 1426 4090 Fragments arranged by size: 4090 1426 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2024 2393 2888 3721 1353 Size: 671 369 495 833 3423 Fragments arranged by size: 3423 833 671 495 369 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4378 4752 4898 387 Size: 1064 138 2789 374 146 1280 Fragments arranged by size: 2789 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2347 2347 Size: 5791 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2573 2891 3028 3854 4451 5238 5506 Size: 393 941 318 137 826 597 787 268 Cuts at: 5506 1239 Size: 1524 Fragments arranged by size: 1524 941 826 787 597 393 318 268 137 EcoRI G'AATT_C Cuts at: 3100 3478 3100 Size: 378 5413 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2782 2782 Size: 5791 FspI TGC'GCA Cuts at: 4297 4297 Size: 5791 HaeII r_GCGC'y Cuts at: 1678 4938 1678 Size: 3260 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2819 2851 4432 4464 4506 Size: 32 135 32 1247 32 1581 32 42 Cuts at: 4506 4538 1373 Size: 32 2626 Fragments arranged by size: 2626 1581 1247 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2935 3135 3381 669 Size: 988 281 997 200 246 3079 Fragments arranged by size: 3079 997 988 281 246 200 HindIII A'AGCT_T Cuts at: 748 748 Size: 5791 HpaI GTT'AAC Cuts at: 3381 3381 Size: 5791 KpnI G_GTAC'C Cuts at: 3116 3116 Size: 5791 MfeI C'AATT_G Cuts at: 3390 3390 Size: 5791 MluI A'CGCG_T Cuts at: 5667 5667 Size: 5791 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2598 4905 5089 1305 Size: 1293 2307 184 2007 Fragments arranged by size: 2307 2007 1293 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2062 2264 2711 10 Size: 54 1425 343 230 202 447 3090 Fragments arranged by size: 3090 1425 447 343 230 202 54 MslI CAynn'nnrTG Cuts at: 518 1166 2087 2099 2177 2414 2420 2504 3749 Size: 648 921 12 78 237 6 84 1245 Cuts at: 3749 4108 4267 518 Size: 359 159 2042 Fragments arranged by size: 2042 1245 921 648 359 237 159 84 78 12 6 MspA1I CmG'CkG Cuts at: 1606 2058 3207 3884 5032 5277 1606 Size: 452 1149 677 1148 245 2120 Fragments arranged by size: 2120 1149 1148 677 452 245 NaeI GCC'GGC Cuts at: 2867 2867 Size: 5791 NcoI C'CATG_G Cuts at: 513 1065 2004 2337 513 Size: 552 939 333 3967 Fragments arranged by size: 3967 939 552 333 NdeI CA'TA_TG Cuts at: 387 387 Size: 5791 NgoMIV G'CCGG_C Cuts at: 2865 2865 Size: 5791 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5791 NotI GC'GGCC_GC Cuts at: 3238 3238 Size: 5791 NspI r_CATG'y Cuts at: 1132 1229 2116 2900 3149 4694 1132 Size: 97 887 784 249 1545 2229 Fragments arranged by size: 2229 1545 887 784 249 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5791 PciI A'CATG_T Cuts at: 4690 4690 Size: 5791 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5791 PmeI GTTT'AAAC Cuts at: 3095 3095 Size: 5791 PmlI CAC'GTG Cuts at: 2352 2412 2812 2352 Size: 60 400 5331 Fragments arranged by size: 5331 400 60 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3860 3892 5373 5405 3860 Size: 32 1481 32 4246 Fragments arranged by size: 4246 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5791 PsiI TTA'TAA Cuts at: 3361 3361 Size: 5791 PstI C_TGCA'G Cuts at: 830 3143 830 Size: 2313 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5791 PvuII CAG'CTG Cuts at: 2058 2058 Size: 5791 SacI G_AGCT'C Cuts at: 2349 2349 Size: 5791 SalI G'TCGA_C Cuts at: 1936 3133 1936 Size: 1197 4594 SbfI CC_TGCA'GG Cuts at: 3143 3143 Size: 5791 ScaI AGT'ACT Cuts at: 3005 3065 4039 5751 3005 Size: 60 974 1712 3045 Fragments arranged by size: 3045 1712 974 60 SfcI C'TryA_G Cuts at: 826 1045 2947 2968 3139 4274 4955 5146 826 Size: 219 1902 21 171 1135 681 191 1471 Fragments arranged by size: 1902 1471 1135 681 219 191 171 21 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5791 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5791 SmaI CCC'GGG Cuts at: 1872 3118 1872 Size: 1246 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3895 4796 5058 820 Size: 197 926 1952 901 262 1553 Fragments arranged by size: 1952 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3582 493 Size: 3089 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5791 SphI G_CATG'C Cuts at: 1132 3149 1132 Size: 2017 3774 SspI AAT'ATT Cuts at: 5 52 3715 5 Size: 47 3663 2081 Fragments arranged by size: 3663 2081 47 StuI AGG'CCT Cuts at: 1517 2990 1517 Size: 1473 4318 StyI C'CwwG_G Cuts at: 513 1065 2004 2337 3072 3230 513 Size: 552 939 333 735 158 3074 Fragments arranged by size: 3074 939 735 552 333 158 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2194 2293 3818 3835 3988 4173 4618 Size: 1255 614 99 1525 17 153 185 445 Cuts at: 4618 325 Size: 1498 Fragments arranged by size: 1525 1498 1255 614 445 185 153 99 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3003 3063 4037 Size: 274 80 33 51 1443 1026 60 974 Cuts at: 4037 5749 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1026 974 274 138 80 60 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5751 5783 5751 Size: 32 5759 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5791 XbaI T'CTAG_A Cuts at: 3127 3127 Size: 5791 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2194 2194 Size: 5791 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5791 XmaI C'CCGG_G Cuts at: 1870 3116 1870 Size: 1246 4545 XmnI GAAnn'nnTTC Cuts at: 2990 3920 2990 Size: 930 4861 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3599 276 Size: 53 83 186 936 2065 2468 Fragments arranged by size: 2468 2065 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I EcoNI EcoO109I EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI