Restriction map for
pFN21AB4171
##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII AccIII AclI AcyI AflII
AgeI Alw44I ApaLI AscI AseI
AsnI Asp700I AspI BbeI BbvCI
BclI BfrI BsaHI BsaWI BseAI
BsiEI BsiWI BsmI BspDI BspEI
BsrFI BssHII Bst1107I BstZ17I Cfr10I
ClaI CpoI DraI DraIII EagI
EclXI Eco52I EcoRI EcoRV EcoT22I
FbaI FseI HgaI Hin1I HindIII
HpaI KasI MfeI MluI MroI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI RsrII SacI
SapI SbfI SfiI SfoI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspI SwaI Tth111I XhoI XmnI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
Acc65I 2208 GGTACC G^GTACC
AccI 1553 GTCGAC GT^[AC][GT]AC
AccII 51 CGCG CG^CG
AcsI 295 AAATTC [GA]^AATT[CT]
AhdI 1096 GACTCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1645 CCTCAGG CC^T[ACGT]AGG
Aor51HI 899 AGCGCT AGC^GCT
ApoI 295 AAATTC [GA]^AATT[CT]
Asp718I 2208 GGTACC G^GTACC
AspEI 1096 GACTCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 2112 GAGCAC G[AT]GC[AT]^C
AviII 1820 TGCGCA TGC^GCA
BalI 987 TGGCCA TGG^CCA
BbrPI 639 CACGTG CAC^GTG
BcgI 2001 GCATTTTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 534 GTATCC GTATCC(6/5)
BglII 1379 AGATCT A^GATCT
BlpI 2707 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 2707 GCTAAGC GC^T[ACGT]AGC
BsaAI 639 CACGTG [CT]AC^GT[GA]
BsaBI 853 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 2112 GAGCAC G[AT]GC[AT]^C
BssSI 1883 CTCGTG CACGAG(-5/-1)
BstAPI 1870 GCAGCCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2237 TTCGAA TT^CGAA
BstEII 3429 GGTCACC G^GT[ACGT]ACC
BstPI 3429 GGTCACC G^GT[ACGT]ACC
BstUI 51 CGCG CG^CG
BstXI 2213 CCACGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1645 CCTCAGG CC^T[ACGT]AGG
CelII 2707 GCTAAGC GC^T[ACGT]AGC
DrdI 2536 GACCCAATAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1096 GACTCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 899 AGCGCT AGC^GCT
Eco81I 1645 CCTCAGG CC^T[ACGT]AGG
EcoO65I 3429 GGTCACC G^GT[ACGT]ACC
FspI 1820 TGCGCA TGC^GCA
HaeII 899 AGCGCT [GA]GCGC^[CT]
KpnI 2208 GGTACC GGTAC^C
KspI 50 CCGCGG CCGC^GG
MamI 853 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 987 TGGCCA TGG^CCA
MscI 987 TGGCCA TGG^CCA
MvnI 51 CGCG CG^CG
NspV 2237 TTCGAA TT^CGAA
PmaCI 639 CACGTG CAC^GTG
PmlI 639 CACGTG CAC^GTG
PpuMI 954 AGGTCCC [GA]G^G[AT]CC[CT]
SacII 50 CCGCGG CCGC^GG
SalI 1553 GTCGAC G^TCGAC
ScaI 38 AGTACT AGT^ACT
SfuI 2237 TTCGAA TT^CGAA
SmaI 543 CCCGGG CCC^GGG
StuI 2048 AGGCCT AGG^CCT
XbaI 552 TCTAGA T^CTAGA
XmaCI 543 CCCGGG C^CCGGG
XmaI 543 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
ApaI 3092 GGGCCC GGGCC^C
3111 GGGCCC
AvrII 700 CCTAGG C^CTAGG
2055 CCTAGG
BamHI 644 GGATCC G^GATCC
722 GGATCC
BlnI 700 CCTAGG C^CTAGG
2055 CCTAGG
BsaI 2534 GAGACC GGTCTC(1/5)
2767 GAGACC
BsgI 607 CTGCAC GTGCAG(16/14)
394 GTGCAG
BsmBI 1165 GAGACG CGTCTC(1/5)
1717 GAGACG
BspHI 581 TCATGA T^CATGA
1490 TCATGA
BspLU11I 198 ACATGT A^CATGT
1829 ACATGT
BspMI 1232 ACCTGC ACCTGC(4/8)
597 GCAGGT
CfoI 900 GCGC GCG^C
1821 GCGC
HapII 544 CCGG C^CGG
2832 CCGG
HhaI 900 GCGC GCG^C
1821 GCGC
HinP1I 900 GCGC G^CGC
1821 GCGC
HpaII 544 CCGG C^CGG
2832 CCGG
MspI 544 CCGG C^CGG
2832 CCGG
NcoI 3039 CCATGG C^CATGG
3225 CCATGG
PflMI 1593 CCATGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3039 CCATGGCTTGG
RcaI 581 TCATGA T^CATGA
1490 TCATGA
SexAI 1535 ACCAGGT A^CC[AT]GGT
1706 ACCTGGT
Van91I 1593 CCATGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3039 CCATGGCTTGG
XcmI 1127 CCATGACCGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2213 CCACGGCCTTGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AlwNI AvaI AvaII BanI BanII
BbsI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaJI BseRI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I Bsp1407I BsrBI BsrDI BsrGI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HincII HindII HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SspBI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII