Restriction map for
pFN21AB4171
(Linear) (Six-base) MAPSORT of: KIBB4171.seq Check: 1578 from: 1 to: 3453
KIBB4171.orf
With 182 enzymes: SgfI *
July 18, 2007 13:37 ..
AccI GT'mk_AC
Cuts at: 0 1554 3453
Size: 1554 1899
Acc65I G'GTAC_C
Cuts at: 0 2208 3453
Size: 2208 1245
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2070 2614 2926 3453
Size: 2070 544 312 527
Fragments arranged by size:
2070 544 527 312
AfeI AGC'GCT
Cuts at: 0 901 3453
Size: 901 2552
AflIII A'CryG_T
Cuts at: 0 198 638 1829 3453
Size: 198 440 1191 1624
Fragments arranged by size:
1624 1191 440 198
AhdI GACnn_n'nnGTC
Cuts at: 0 1101 3453
Size: 1101 2352
AleI CACnn'nnGTG
Cuts at: 0 3436 3453
Size: 3436 17
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2735 3370 3409 3453
Size: 2735 635 39 44
Fragments arranged by size:
2735 635 44 39
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 505 537 1942 1974 3453
Size: 505 32 1405 32 1479
Fragments arranged by size:
1479 1405 505 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 502 2871 3019 3064 3453
Size: 502 2369 148 45 389
Fragments arranged by size:
2369 502 389 148 45
ApaI G_GGCC'C
Cuts at: 0 3096 3115 3453
Size: 3096 19 338
Fragments arranged by size:
3096 338 19
ApoI r'AATT_y
Cuts at: 0 295 3453
Size: 295 3158
AvaI C'yCGr_G
Cuts at: 0 543 1339 2147 2366 2414 3414 3453
Size: 543 796 808 219 48 1000 39
Fragments arranged by size:
1000 808 796 543 219 48 39
AvrII C'CTAG_G
Cuts at: 0 700 2055 3453
Size: 700 1355 1398
Fragments arranged by size:
1398 1355 700
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2836 2869 3453
Size: 2836 33 584
Fragments arranged by size:
2836 584 33
BamHI G'GATC_C
Cuts at: 0 644 722 3453
Size: 644 78 2731
Fragments arranged by size:
2731 644 78
BanI G'GyrC_C
Cuts at: 0 1123 1623 1710 2208 3296 3385 3453
Size: 1123 500 87 498 1088 89 68
Fragments arranged by size:
1123 1088 500 498 89 87 68
BanII G_rGCy'C
Cuts at: 0 449 1037 1335 2405 2928 3096 3115 3180
Size: 449 588 298 1070 523 168 19 65
Cuts at: 3180 3421 3453
Size: 241 32
Fragments arranged by size:
1070 588 523 449 298 241 168 65
32 19
BbsI GAAGACnn'nnnn_
Cuts at: 0 770 1016 2005 2030 2632 3453
Size: 770 246 989 25 602 821
Fragments arranged by size:
989 821 770 602 246 25
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1990 2024 3453
Size: 1990 34 1429
Fragments arranged by size:
1990 1429 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 545 3453
Size: 545 2908
BglI GCCn_nnn'nGGC
Cuts at: 0 2128 2686 3331 3453
Size: 2128 558 645 122
Fragments arranged by size:
2128 645 558 122
BglII A'GATC_T
Cuts at: 0 1379 3453
Size: 1379 2074
BlpI GC'TnA_GC
Cuts at: 0 2708 3453
Size: 2708 745
Bme1580I G_kGCm'C
Cuts at: 0 436 3096 3115 3299 3453
Size: 436 2660 19 184 154
Fragments arranged by size:
2660 436 184 154 19
BmrI ACTGGGnnnn_n'
Cuts at: 0 60 621 1105 3353 3453
Size: 60 561 484 2248 100
Fragments arranged by size:
2248 561 484 100 60
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1761 2205 3453
Size: 1761 444 1248
Fragments arranged by size:
1761 1248 444
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1358 1939 2056 2836 2869 3041 3083 3194
Size: 1358 581 117 780 33 172 42 111
Cuts at: 3194 3383 3453
Size: 189 70
Fragments arranged by size:
1358 780 581 189 172 117 111 70
42 33
Bpu10I CC'TnA_GC
Cuts at: 0 1724 2333 3453
Size: 1724 609 1120
Fragments arranged by size:
1724 1120 609
BsaI GGTCTCn'nnnn_
Cuts at: 0 2528 2761 3453
Size: 2528 233 692
Fragments arranged by size:
2528 692 233
BsaAI yAC'GTr
Cuts at: 0 641 3453
Size: 641 2812
BsaBI GATnn'nnATC
Cuts at: 0 857 3453
Size: 857 2596
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 505 535 781 811 3453
Size: 505 30 246 30 2642
Fragments arranged by size:
2642 505 246 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1015 2197 2359 2452 2497 2824 3248 3453
Size: 1015 1182 162 93 45 327 424 205
Fragments arranged by size:
1182 1015 424 327 205 162 93 45
BseYI C'CCAG_C
Cuts at: 0 648 1279 1678 2996 3022 3182 3453
Size: 648 631 399 1318 26 160 271
Fragments arranged by size:
1318 648 631 399 271 160 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 415 592 3453
Size: 415 177 2861
Fragments arranged by size:
2861 415 177
BsiHKAI G_wGCw'C
Cuts at: 0 2116 3453
Size: 2116 1337
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1159 1711 3453
Size: 1159 552 1742
Fragments arranged by size:
1742 1159 552
Bsp1286I G_dGCh'C
Cuts at: 0 436 449 1037 1335 2116 2405 2928 3096
Size: 436 13 588 298 781 289 523 168
Cuts at: 3096 3115 3180 3299 3421 3453
Size: 19 65 119 122 32
Fragments arranged by size:
781 588 523 436 298 289 168 122
119 65 32 19 13
BspHI T'CATG_A
Cuts at: 0 581 1490 3453
Size: 581 909 1963
Fragments arranged by size:
1963 909 581
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 588 1241 3453
Size: 588 653 2212
Fragments arranged by size:
2212 653 588
BsrBI CCG'CTC
Cuts at: 0 216 1326 2337 2351 2640 3453
Size: 216 1110 1011 14 289 813
Fragments arranged by size:
1110 1011 813 289 216 14
BsrDI GCAATG_nn'
Cuts at: 0 213 363 2306 3453
Size: 213 150 1943 1147
Fragments arranged by size:
1943 1147 213 150
BsrGI T'GTAC_A
Cuts at: 0 159 1832 1934 3453
Size: 159 1673 102 1519
Fragments arranged by size:
1673 1519 159 102
BssSI C'ACGA_G
Cuts at: 0 1883 3453
Size: 1883 1570
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1876 3453
Size: 1876 1577
BstBI TT'CG_AA
Cuts at: 0 2238 3453
Size: 2238 1215
BstEII G'GTnAC_C
Cuts at: 0 3429 3453
Size: 3429 24
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2220 3453
Size: 2220 1233
BstYI r'GATC_y
Cuts at: 0 644 722 1379 3453
Size: 644 78 657 2074
Fragments arranged by size:
2074 657 644 78
Bsu36I CC'TnA_GG
Cuts at: 0 1646 3453
Size: 1646 1807
BtgI C'CryG_G
Cuts at: 0 50 2213 3039 3225 3453
Size: 50 2163 826 186 228
Fragments arranged by size:
2163 826 228 186 50
BtsI GCAGTG_nn'
Cuts at: 0 2307 2705 3093 3287 3370 3453
Size: 2307 398 388 194 83 83
Fragments arranged by size:
2307 398 388 194 83 83
DrdI GACnn_nn'nnGTC
Cuts at: 0 2542 3453
Size: 2542 911
EaeI y'GGCC_r
Cuts at: 0 987 2687 2873 3453
Size: 987 1700 186 580
Fragments arranged by size:
1700 987 580 186
EarI CTCTTCn'nnn_
Cuts at: 0 554 580 886 992 1052 2198 2518 3453
Size: 554 26 306 106 60 1146 320 935
Fragments arranged by size:
1146 935 554 320 306 106 60 26
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1565 2290 3453
Size: 1565 725 1163
Fragments arranged by size:
1565 1163 725
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1358 1939 2056 2070 2614 2836 2869 2926
Size: 1358 581 117 14 544 222 33 57
Cuts at: 2926 3041 3083 3194 3383 3453
Size: 115 42 111 189 70
Fragments arranged by size:
1358 581 544 222 189 117 115 111
70 57 42 33 14
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1018 1237 1778 3453
Size: 1018 219 541 1675
Fragments arranged by size:
1675 1018 541 219
EcoO109I rG'GnC_Cy
Cuts at: 0 955 1570 2059 3092 3093 3112 3453
Size: 955 615 489 1033 1 19 341
Fragments arranged by size:
1033 955 615 489 341 19 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1456 3453
Size: 1456 1997
FspI TGC'GCA
Cuts at: 0 1822 3453
Size: 1822 1631
HaeII r_GCGC'y
Cuts at: 0 903 3453
Size: 903 2550
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1021 1053 1087 1119 1209 1241 1597 1629
Size: 1021 32 34 32 90 32 356 32
Cuts at: 1629 2500 2532 2910 2942 3453
Size: 871 32 378 32 511
Fragments arranged by size:
1021 871 511 378 356 90 34 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1083 1555 3143 3453
Size: 1083 472 1588 310
Fragments arranged by size:
1588 1083 472 310
KpnI G_GTAC'C
Cuts at: 0 2212 3453
Size: 2212 1241
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 709 2765 2967 3453
Size: 709 2056 202 486
Fragments arranged by size:
2056 709 486 202
MscI TGG'CCA
Cuts at: 0 989 3453
Size: 989 2464
MslI CAynn'nnrTG
Cuts at: 0 1592 1828 3436 3453
Size: 1592 236 1608 17
Fragments arranged by size:
1608 1592 236 17
MspA1I CmG'CkG
Cuts at: 0 52 286 2671 2904 3172 3293 3337 3453
Size: 52 234 2385 233 268 121 44 116
Fragments arranged by size:
2385 268 234 233 121 116 52 44
NcoI C'CATG_G
Cuts at: 0 3039 3225 3453
Size: 3039 186 228
Fragments arranged by size:
3039 228 186
NspI r_CATG'y
Cuts at: 0 202 1806 1833 1969 3453
Size: 202 1604 27 136 1484
Fragments arranged by size:
1604 1484 202 136 27
PciI A'CATG_T
Cuts at: 0 198 1829 3453
Size: 198 1631 1624
Fragments arranged by size:
1631 1624 198
PflMI CCAn_nnn'nTGG
Cuts at: 0 1599 3045 3453
Size: 1599 1446 408
Fragments arranged by size:
1599 1446 408
PfoI T'CCnGG_A
Cuts at: 0 725 1916 3099 3453
Size: 725 1191 1183 354
Fragments arranged by size:
1191 1183 725 354
PmlI CAC'GTG
Cuts at: 0 641 3453
Size: 641 2812
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 505 537 3453
Size: 505 32 2916
Fragments arranged by size:
2916 505 32
PpuMI rG'GwC_Cy
Cuts at: 0 955 3453
Size: 955 2498
PspOMI G'GGCC_C
Cuts at: 0 3092 3111 3453
Size: 3092 19 342
Fragments arranged by size:
3092 342 19
PstI C_TGCA'G
Cuts at: 0 1634 1872 3150 3453
Size: 1634 238 1278 303
Fragments arranged by size:
1634 1278 303 238
PvuII CAG'CTG
Cuts at: 0 286 2904 3172 3337 3453
Size: 286 2618 268 165 116
Fragments arranged by size:
2618 286 268 165 116
SacII CC_GC'GG
Cuts at: 0 53 3453
Size: 53 3400
SalI G'TCGA_C
Cuts at: 0 1553 3453
Size: 1553 1900
ScaI AGT'ACT
Cuts at: 0 40 3453
Size: 40 3413
SexAI A'CCwGG_T
Cuts at: 0 1535 1706 3453
Size: 1535 171 1747
Fragments arranged by size:
1747 1535 171
SfcI C'TryA_G
Cuts at: 0 1630 1868 2432 3065 3146 3453
Size: 1630 238 564 633 81 307
Fragments arranged by size:
1630 633 564 307 238 81
SmaI CCC'GGG
Cuts at: 0 545 3453
Size: 545 2908
StuI AGG'CCT
Cuts at: 0 2050 3453
Size: 2050 1403
StyI C'CwwG_G
Cuts at: 0 389 700 1673 2055 2219 2405 3039 3225
Size: 389 311 973 382 164 186 634 186
Cuts at: 3225 3453
Size: 228
Fragments arranged by size:
973 634 389 382 311 228 186 186
164
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 144 1147 2278 3314 3453
Size: 144 1003 1131 1036 139
Fragments arranged by size:
1131 1036 1003 144 139
TatI w'GTAC_w
Cuts at: 0 38 159 1832 1934 3453
Size: 38 121 1673 102 1519
Fragments arranged by size:
1673 1519 121 102 38
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3020 3052 3453
Size: 3020 32 401
Fragments arranged by size:
3020 401 32
XbaI T'CTAG_A
Cuts at: 0 552 3453
Size: 552 2901
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1134 2220 3453
Size: 1134 1086 1233
Fragments arranged by size:
1233 1134 1086
XmaI C'CCGG_G
Cuts at: 0 543 3453
Size: 543 2910
Enzymes that do cut:
AccI Acc65I AcuI AfeI AflIII AhdI AleI AlfI
AloI AlwNI ApaI ApoI AvaI AvrII BaeI BamHI
BanI BanII BbsI BcgI BciVI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BsaI BsaAI BsaBI
BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtsI DrdI EaeI EarI
EciI Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I
HincII KpnI MmeI MscI MslI MspA1I NcoI NspI
PciI PflMI PfoI PmlI PpiI PpuMI PspOMI PstI
PvuII SacII SalI ScaI SexAI SfcI SmaI StuI
StyI TaqII TatI TstI XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AclI AflII AgeI ApaLI AscI AseI
AsiSI BbeI BbvCI BclI BfrBI BmgBI BmtI BpuEI
BsaHI BsaWI BsiEI BsiWI BsmI BspEI BsrFI BssHII
BstZ17I BtgZI ClaI CspCI DraI DraIII EagI EcoICRI
EcoRI EcoRV FseI FspAI HindIII HpaI KasI MfeI
MluI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PmeI PshAI PsiI PspXI PsrI
PvuI RsrII SacI SanDI SapI SbfI SfiI SfoI
SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI
SwaI Tth111I XhoI XmnI ZraI