Restriction map for
pFN21AB4141
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# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AflIII AgeI AhdI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
AvrII BamHI BbrPI BbvCI BcgI
BciVI BclI BfrI BglII BlnI
BsaBI BsiWI BsmBI BsmI BspDI
BspLU11I BssHII Bst1107I BstBI BstZ17I
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco52I EcoRV EcoT22I FbaI
FseI FspI HpaI KpnI KspI
MamI MfeI MluI MunI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SfuI SnaBI SpeI Sse8387I SspI
SwaI TfiI XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccII 168 CGCG CG^CG
AccIII 501 TCCGGA T^CCGGA
AcsI 1251 GAATTC [GA]^AATT[CT]
Alw44I 374 GTGCAC G^TGCAC
Aor51HI 986 AGCGCT AGC^GCT
ApaLI 374 GTGCAC G^TGCAC
ApoI 1251 GAATTC [GA]^AATT[CT]
AspHI 374 GTGCAC G[AT]GC[AT]^C
AspI 56 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 616 GGCGCC GGCGC^C
BfaI 331 CTAG C^TAG
BsaAI 226 TACGTG [CT]AC^GT[GA]
BseAI 501 TCCGGA T^CCGGA
BsgI 2023 GTGCAG GTGCAG(16/14)
BsiEI 2168 CGGTCG CG[GA][CT]^CG
BsiHKAI 374 GTGCAC G[AT]GC[AT]^C
BspEI 501 TCCGGA T^CCGGA
BspHI 2048 TCATGA T^CATGA
BsrFI 613 ACCGGC [GA]^CCGG[CT]
BstAPI 338 GCATGGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1968 GGTCACC G^GT[ACGT]ACC
BstPI 1968 GGTCACC G^GT[ACGT]ACC
BstUI 168 CGCG CG^CG
Cfr10I 613 ACCGGC [GA]^CCGG[CT]
DraI 1320 TTTAAA TTT^AAA
DraIII 379 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 986 AGCGCT AGC^GCT
EcoO65I 1968 GGTCACC G^GT[ACGT]ACC
EcoRI 1251 GAATTC G^AATTC
HincII 1009 GTTGAC GT[CT]^[GA]AC
HindII 1009 GTTGAC GT[CT]^[GA]AC
HindIII 13 AAGCTT A^AGCTT
KasI 616 GGCGCC G^GCGCC
MaeI 331 CTAG C^TAG
MaeII 227 ACGT A^CGT
MroI 501 TCCGGA T^CCGGA
MseI 1321 TTAA T^TAA
MvnI 168 CGCG CG^CG
NarI 616 GGCGCC GG^CGCC
PflFI 56 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 681 CCAGATCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 2048 TCATGA T^CATGA
SexAI 57 ACCAGGT A^CC[AT]GGT
SfiI 929 GGCCGAAGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 616 GGCGCC GGC^GCC
SgrAI 612 CACCGGCG C[GA]^CCGG[CT]G
SmaI 1462 CCCGGG CCC^GGG
SphI 1421 GCATGC GCATG^C
Tru9I 1321 TTAA T^TAA
Tth111I 56 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 681 CCAGATCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 1462 CCCGGG C^CCGGG
XmaI 1462 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
BanI 616 GGCGCC G^G[CT][GA]CC
1867 GGCACC
BglI 733 GCCAGTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
930 GCCGAAGTGGC
BlpI 1833 GCTGAGC GC^T[ACGT]AGC
2065 GCTGAGC
Bpu1102I 1833 GCTGAGC GC^T[ACGT]AGC
2065 GCTGAGC
BsaWI 8 ACCGGA [AT]^CCGG[AT]
501 TCCGGA
Bsp1407I 1655 TGTACA T^GTACA
2240 TGTACA
BspMI 1897 ACCTGC ACCTGC(4/8)
948 GCAGGT
BsrBI 1821 CCGCTC CCGCTC(-3/-3)
30 GAGCGG
BsrDI 2061 CATTGC GCAATG(2/0)
956 GCAATG
BsrGI 1655 TGTACA T^GTACA
2240 TGTACA
BsrI 734 CCAGT ACTGG(1/-1)
1539 CCAGT
BstXI 1121 CCAATTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1871 CCAGCCTGGTGG
BstYI 1730 AGATCC [GA]^GATC[CT]
2177 AGATCC
CelII 1833 GCTGAGC GC^T[ACGT]AGC
2065 GCTGAGC
EarI 1291 CTCTTC CTCTTC(1/4)
922 GAAGAG
Ksp632I 1291 CTCTTC CTCTTC(1/4)
922 GAAGAG
MaeIII 551 GTCAC ^GT[ACGT]AC
1969 GTCAC
MflI 1730 AGATCC [GA]^GATC[CT]
2177 AGATCC
NspI 35 GCATGT [GA]CATG^[CT]
1421 GCATGC
SspBI 1655 TGTACA T^GTACA
2240 TGTACA
StuI 788 AGGCCT AGG^CCT
1553 AGGCCT
Tsp45I 551 GTCAC ^GT[GC]AC
1969 GTCAC
XhoII 1730 AGATCC [GA]^GATC[CT]
2177 AGATCC
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# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AocI ApaI AvaI AvaII
BalI BanII BbsI BbvI BcnI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BssKI BssSI
BstF5I BstNI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MboI MboII MluNI MnlI MscI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TseI Tsp509I TspRI
TthHB8I XcmI