Restriction map for
pFN21AB4141
(Linear) (Six-base) MAPSORT of: KIBB4141.seq Check: 3977 from: 1 to: 2247
KIBB4141.orf
With 182 enzymes: SgfI *
July 18, 2007 13:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1906 2247
Size: 1906 341
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 482 1542 1942 2001 2247
Size: 482 1060 400 59 246
Fragments arranged by size:
1060 482 400 246 59
AfeI AGC'GCT
Cuts at: 0 988 2247
Size: 988 1259
AleI CACnn'nnGTG
Cuts at: 0 557 2247
Size: 557 1690
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1244 1720 2247
Size: 1244 476 527
Fragments arranged by size:
1244 527 476
AlwNI CAG_nnn'CTG
Cuts at: 0 64 772 1275 2247
Size: 64 708 503 972
Fragments arranged by size:
972 708 503 64
ApaI G_GGCC'C
Cuts at: 0 439 1079 1470 2247
Size: 439 640 391 777
Fragments arranged by size:
777 640 439 391
ApaLI G'TGCA_C
Cuts at: 0 374 2247
Size: 374 1873
ApoI r'AATT_y
Cuts at: 0 1251 2247
Size: 1251 996
AvaI C'yCGr_G
Cuts at: 0 85 127 592 748 862 1462 1824 2247
Size: 85 42 465 156 114 600 362 423
Fragments arranged by size:
600 465 423 362 156 114 85 42
BanI G'GyrC_C
Cuts at: 0 616 1867 2247
Size: 616 1251 380
Fragments arranged by size:
1251 616 380
BanII G_rGCy'C
Cuts at: 0 86 166 439 515 1079 1470 1768 2247
Size: 86 80 273 76 564 391 298 479
Fragments arranged by size:
564 479 391 298 273 86 80 76
BbeI G_GCGC'C
Cuts at: 0 620 2247
Size: 620 1627
BbsI GAAGACnn'nnnn_
Cuts at: 0 1097 1613 2194 2247
Size: 1097 516 581 53
Fragments arranged by size:
1097 581 516 53
BglI GCCn_nnn'nGGC
Cuts at: 0 739 936 2247
Size: 739 197 1311
Fragments arranged by size:
1311 739 197
BlpI GC'TnA_GC
Cuts at: 0 1834 2066 2247
Size: 1834 232 181
Fragments arranged by size:
1834 232 181
Bme1580I G_kGCm'C
Cuts at: 0 128 378 439 1079 1470 2247
Size: 128 250 61 640 391 777
Fragments arranged by size:
777 640 391 250 128 61
BmrI ACTGGGnnnn_n'
Cuts at: 0 1533 2247
Size: 1533 714
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 198 676 985 1732 2247
Size: 157 41 478 309 747 515
Fragments arranged by size:
747 515 478 309 157 41
Bpu10I CC'TnA_GC
Cuts at: 0 761 2247
Size: 761 1486
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 544 1632 1984 2247
Size: 37 507 1088 352 263
Fragments arranged by size:
1088 507 352 263 37
BsaI GGTCTCn'nnnn_
Cuts at: 0 48 875 1447 2224 2247
Size: 48 827 572 777 23
Fragments arranged by size:
827 777 572 48 23
BsaAI yAC'GTr
Cuts at: 0 228 2247
Size: 228 2019
BsaHI Gr'CG_yC
Cuts at: 0 617 1416 2078 2247
Size: 617 799 662 169
Fragments arranged by size:
799 662 617 169
BsaWI w'CCGG_w
Cuts at: 0 8 501 2247
Size: 8 493 1746
Fragments arranged by size:
1746 493 8
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 259 262 289 292 295 370 430 610
Size: 259 3 27 3 3 75 60 180
Cuts at: 610 682 2206 2247
Size: 72 1524 41
Fragments arranged by size:
1524 259 180 75 72 60 41 27
3 3 3
BseYI C'CCAG_C
Cuts at: 0 820 1139 1526 1760 2010 2247
Size: 820 319 387 234 250 237
Fragments arranged by size:
820 387 319 250 237 234
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2044 2247
Size: 2044 203
BsiEI CG_ry'CG
Cuts at: 0 2171 2247
Size: 2171 76
BsiHKAI G_wGCw'C
Cuts at: 0 378 2247
Size: 378 1869
Bsp1286I G_dGCh'C
Cuts at: 0 86 128 166 378 439 515 1079 1470
Size: 86 42 38 212 61 76 564 391
Cuts at: 1470 1768 2247
Size: 298 479
Fragments arranged by size:
564 479 391 298 212 86 76 61
42 38
BspEI T'CCGG_A
Cuts at: 0 501 2247
Size: 501 1746
BspHI T'CATG_A
Cuts at: 0 2048 2247
Size: 2048 199
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 939 1906 2247
Size: 939 967 341
Fragments arranged by size:
967 939 341
BsrBI CCG'CTC
Cuts at: 0 32 1823 2247
Size: 32 1791 424
Fragments arranged by size:
1791 424 32
BsrDI GCAATG_nn'
Cuts at: 0 963 2060 2247
Size: 963 1097 187
Fragments arranged by size:
1097 963 187
BsrFI r'CCGG_y
Cuts at: 0 613 2247
Size: 613 1634
BsrGI T'GTAC_A
Cuts at: 0 1655 2240 2247
Size: 1655 585 7
Fragments arranged by size:
1655 585 7
BssSI C'ACGA_G
Cuts at: 0 454 829 943 2137 2247
Size: 454 375 114 1194 110
Fragments arranged by size:
1194 454 375 114 110
BstAPI GCAn_nnn'nTGC
Cuts at: 0 344 2247
Size: 344 1903
BstEII G'GTnAC_C
Cuts at: 0 1968 2247
Size: 1968 279
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1128 1878 2247
Size: 1128 750 369
Fragments arranged by size:
1128 750 369
BstYI r'GATC_y
Cuts at: 0 1730 2177 2247
Size: 1730 447 70
Fragments arranged by size:
1730 447 70
Bsu36I CC'TnA_GG
Cuts at: 0 272 314 634 773 2247
Size: 272 42 320 139 1474
Fragments arranged by size:
1474 320 272 139 42
BtgI C'CryG_G
Cuts at: 0 447 1778 1973 2247
Size: 447 1331 195 274
Fragments arranged by size:
1331 447 274 195
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1362 1518 2247
Size: 1362 156 729
Fragments arranged by size:
1362 729 156
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1022 1043 1057 1078 2247
Size: 1022 21 14 21 1169
Fragments arranged by size:
1169 1022 21 21 14
DraI TTT'AAA
Cuts at: 0 1322 2247
Size: 1322 925
DraIII CAC_nnn'GTG
Cuts at: 0 384 2247
Size: 384 1863
EaeI y'GGCC_r
Cuts at: 0 154 450 486 1409 1548 1790 1990 2126
Size: 154 296 36 923 139 242 200 136
Cuts at: 2126 2247
Size: 121
Fragments arranged by size:
923 296 242 200 154 139 136 121
36
EarI CTCTTCn'nnn_
Cuts at: 0 917 1297 2247
Size: 917 380 950
Fragments arranged by size:
950 917 380
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 198 482 676 985 1542 1732 1942
Size: 157 41 284 194 309 557 190 210
Cuts at: 1942 2001 2247
Size: 59 246
Fragments arranged by size:
557 309 284 246 210 194 190 157
59 41
EcoNI CCTnn'n_nnAGG
Cuts at: 0 271 764 862 2247
Size: 271 493 98 1385
Fragments arranged by size:
1385 493 271 98
EcoO109I rG'GnC_Cy
Cuts at: 0 61 132 264 651 769 914 1075 1076
Size: 61 71 132 387 118 145 161 1
Cuts at: 1076 1466 1917 2247
Size: 390 451 330
Fragments arranged by size:
451 390 387 330 161 145 132 118
71 61 1
EcoRI G'AATT_C
Cuts at: 0 1251 2247
Size: 1251 996
HaeII r_GCGC'y
Cuts at: 0 620 734 990 2247
Size: 620 114 256 1257
Fragments arranged by size:
1257 620 256 114
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 45 77 196 202 228 234 499 531
Size: 45 32 119 6 26 6 265 32
Cuts at: 531 2063 2095 2247
Size: 1532 32 152
Fragments arranged by size:
1532 265 152 119 45 32 32 32
26 6 6
HincII GTy'rAC
Cuts at: 0 1011 2247
Size: 1011 1236
HindIII A'AGCT_T
Cuts at: 0 13 2247
Size: 13 2234
KasI G'GCGC_C
Cuts at: 0 616 2247
Size: 616 1631
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 20 1496 2151 2186 2247
Size: 20 1476 655 35 61
Fragments arranged by size:
1476 655 61 35 20
MscI TGG'CCA
Cuts at: 0 1411 1792 2128 2247
Size: 1411 381 336 119
Fragments arranged by size:
1411 381 336 119
MslI CAynn'nnrTG
Cuts at: 0 557 1177 1399 2247
Size: 557 620 222 848
Fragments arranged by size:
848 620 557 222
MspA1I CmG'CkG
Cuts at: 0 812 1143 1710 1958 2010 2238 2247
Size: 812 331 567 248 52 228 9
Fragments arranged by size:
812 567 331 248 228 52 9
NarI GG'CG_CC
Cuts at: 0 617 2247
Size: 617 1630
NspI r_CATG'y
Cuts at: 0 39 1425 2247
Size: 39 1386 822
Fragments arranged by size:
1386 822 39
PflMI CCAn_nnn'nTGG
Cuts at: 0 687 2247
Size: 687 1560
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 223 255 2247
Size: 223 32 1992
Fragments arranged by size:
1992 223 32
PpuMI rG'GwC_Cy
Cuts at: 0 61 651 914 2247
Size: 61 590 263 1333
Fragments arranged by size:
1333 590 263 61
PspOMI G'GGCC_C
Cuts at: 0 435 1075 1466 2247
Size: 435 640 391 781
Fragments arranged by size:
781 640 435 391
PstI C_TGCA'G
Cuts at: 0 817 1709 2044 2247
Size: 817 892 335 203
Fragments arranged by size:
892 817 335 203
PvuII CAG'CTG
Cuts at: 0 812 1143 1710 1958 2010 2247
Size: 812 331 567 248 52 237
Fragments arranged by size:
812 567 331 248 237 52
SexAI A'CCwGG_T
Cuts at: 0 57 2247
Size: 57 2190
SfcI C'TryA_G
Cuts at: 0 782 813 1705 2040 2247
Size: 782 31 892 335 207
Fragments arranged by size:
892 782 335 207 31
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 936 2247
Size: 936 1311
SfoI GGC'GCC
Cuts at: 0 618 2247
Size: 618 1629
SgrAI Cr'CCGG_yG
Cuts at: 0 613 2247
Size: 613 1634
SmaI CCC'GGG
Cuts at: 0 1464 2247
Size: 1464 783
SmlI C'TyrA_G
Cuts at: 0 16 523 1611 1999 2247
Size: 16 507 1088 388 248
Fragments arranged by size:
1088 507 388 248 16
SphI G_CATG'C
Cuts at: 0 1425 2247
Size: 1425 822
StuI AGG'CCT
Cuts at: 0 790 1555 2247
Size: 790 765 692
Fragments arranged by size:
790 765 692
StyI C'CwwG_G
Cuts at: 0 110 973 1902 2247
Size: 110 863 929 345
Fragments arranged by size:
929 863 345 110
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 813 1153 1318 1660 2157 2247
Size: 813 340 165 342 497 90
Fragments arranged by size:
813 497 342 340 165 90
TatI w'GTAC_w
Cuts at: 0 1655 2240 2247
Size: 1655 585 7
Fragments arranged by size:
1655 585 7
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 169 201 2247
Size: 169 32 2046
Fragments arranged by size:
2046 169 32
Tth111I GACn'n_nGTC
Cuts at: 0 59 2247
Size: 59 2188
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 202 544 1785 2247
Size: 202 342 1241 462
Fragments arranged by size:
1241 462 342 202
XmaI C'CCGG_G
Cuts at: 0 1462 2247
Size: 1462 785
Enzymes that do cut:
AarI AcuI AfeI AleI AlfI AlwNI ApaI ApaLI
ApoI AvaI BanI BanII BbeI BbsI BglI BlpI
Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstEII BstXI BstYI Bsu36I BtgI BtgZI CspCI DraI
DraIII EaeI EarI Eco57MI EcoNI EcoO109I EcoRI HaeII
Hin4I HincII HindIII KasI MmeI MscI MslI MspA1I
NarI NspI PflMI PpiI PpuMI PspOMI PstI PvuII
SexAI SfcI SfiI SfoI SgrAI SmaI SmlI SphI
StuI StyI TaqII TatI TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AloI AscI AseI AsiSI AvrII BaeI BamHI BbvCI
BcgI BciVI BclI BfrBI BglII BmgBI BmtI BplI
BsaBI BsaXI BsiWI BsmI BsmBI BssHII BstBI BstZ17I
BtsI ClaI DrdI EagI EciI EcoICRI EcoRV FalI
FseI FspI FspAI HpaI KpnI MfeI MluI NaeI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PfoI PmeI PmlI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SgfI SnaBI SpeI SrfI SspI SwaI
XbaI XhoI XmnI ZraI