Restriction map for
pFN21AB4140
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# NO CUT ENZYMES ( 97 enzymes) #
##################################
Acc65I AclI AflII AgeI AhdI
AlwI Aor51HI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvrII
BamHI BbeI BciVI BclI BfrI
BlnI BlpI Bpu1102I BsaBI BsiWI
BsmBI BsmI BspDI BsrBI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I CelII ClaI CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRV EcoT22I FbaI
HindIII HpaI KasI KpnI MamI
MfeI MluI MslI MunI NarI
NcoI NdeI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SacI SapI SbfI ScaI
SexAI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
SwaI Tth111I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AatII 256 GACGTC GACGT^C
AccIII 722 TCCGGA T^CCGGA
Alw44I 1327 GTGCAC G^TGCAC
ApaLI 1327 GTGCAC G^TGCAC
AviII 644 TGCGCA TGC^GCA
BbrPI 477 CACGTG CAC^GTG
BbsI 253 GAAGAC GAAGAC(2/6)
BglII 893 AGATCT A^GATCT
BpuAI 253 GAAGAC GAAGAC(2/6)
BsaI 310 GAGACC GGTCTC(1/5)
BsaWI 722 TCCGGA [AT]^CCGG[AT]
BseAI 722 TCCGGA T^CCGGA
BsiEI 94 CGGTCG CG[GA][CT]^CG
Bsp1407I 2180 TGTACA T^GTACA
BspEI 722 TCCGGA T^CCGGA
BspHI 471 TCATGA T^CATGA
BspLU11I 1782 ACATGT A^CATGT
BsrDI 1949 CATTGC GCAATG(2/0)
BsrGI 2180 TGTACA T^GTACA
BstAPI 429 GCAGACGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 2024 CCAAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 893 AGATCT [GA]^GATC[CT]
DraI 1111 TTTAAA TTT^AAA
EcoRI 1722 GAATTC G^AATTC
FspI 644 TGCGCA TGC^GCA
KspI 91 CCGCGG CCGC^GG
MaeIII 691 GTCAC ^GT[ACGT]AC
MflI 893 AGATCT [GA]^GATC[CT]
MroI 722 TCCGGA T^CCGGA
MseI 1112 TTAA T^TAA
NheI 1357 GCTAGC G^CTAGC
NspI 1782 ACATGT [GA]CATG^[CT]
PmaCI 477 CACGTG CAC^GTG
PmlI 477 CACGTG CAC^GTG
RcaI 471 TCATGA T^CATGA
SacII 91 CCGCGG CCGC^GG
SfaNI 218 GCATC GCATC(5/9)
SfiI 2218 GGCCATGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 2180 TGTACA T^GTACA
StuI 374 AGGCCT AGG^CCT
Tru9I 1112 TTAA T^TAA
Tsp45I 691 GTCAC ^GT[GC]AC
XhoII 893 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcsI 1094 AAATTC [GA]^AATT[CT]
1722 GAATTC
AcyI 256 GACGTC G[GA]^CG[CT]C
454 GACGCC
AfaI 558 GTAC GT^AC
2181 GTAC
AflIII 476 ACACGT A^C[GA][CT]GT
1782 ACATGT
AocI 1801 CCTGAGG CC^T[ACGT]AGG
2044 CCTGAGG
ApaI 1989 GGGCCC GGGCC^C
2207 GGGCCC
ApoI 1094 AAATTC [GA]^AATT[CT]
1722 GAATTC
AvaI 740 CCCGAG C^[CT]CG[GA]G
2063 CCCGAG
BanI 1141 GGTGCC G^G[CT][GA]CC
1468 GGTGCC
BbvCI 304 GCTGAGG CCTCAGC(-5/-2)
1792 GCTGAGG
BcgI 218 GCATCGCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1248 GCAGGCAGCTCG
BsaAI 477 CACGTG [CT]AC^GT[GA]
1324 TACGTG
BsaHI 256 GACGTC G[GA]^CG[CT]C
454 GACGCC
BsoBI 740 CCCGAG C^[CT]CG[GA]G
2063 CCCGAG
Bsu36I 1801 CCTGAGG CC^T[ACGT]AGG
2044 CCTGAGG
DsaI 91 CCGCGG C^C[GA][CT]GG
185 CCGTGG
EarI 553 GAAGAG CTCTTC(1/4)
1083 GAAGAG
Eco81I 1801 CCTGAGG CC^T[ACGT]AGG
2044 CCTGAGG
FseI 272 GGCCGGCC GGCCGG^CC
1027 GGCCGGCC
Hin1I 256 GACGTC G[GA]^CG[CT]C
454 GACGCC
HincII 1811 GTCGAC GT[CT]^[GA]AC
2035 GTCGAC
HindII 1811 GTCGAC GT[CT]^[GA]AC
2035 GTCGAC
Ksp632I 553 GAAGAG CTCTTC(1/4)
1083 GAAGAG
PflMI 409 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
863 CCACTGTTTGG
RsaI 558 GTAC GT^AC
2181 GTAC
SalI 1811 GTCGAC G^TCGAC
2035 GTCGAC
Van91I 409 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
863 CCACTGTTTGG
XcmI 890 CCAAGATCTACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1388 CCATGCACCGGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AccI AccII AciI AluI AlwNI
AspHI AvaII BalI BanII BbvI
BcnI BfaI BglI BmyI BpmI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrFI BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI MaeI MaeII MboI MboII
MluNI MnlI MscI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV PleI
PpuMI PstI PvuII Sau3AI Sau96I
ScrFI SfcI SmlI StyI TaqI
TfiI TseI Tsp509I TspRI TthHB8I