(Circular) (Six-base) MAPSORT of: pFN21AB4140.seq Check: 6550 from: 1 to: 6946 pFN21AB4140 6946 bp With 182 enzymes: SgfI * February 27, 2009 14:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3461 4090 3461 Size: 629 6317 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2265 4756 278 Size: 53 83 186 936 729 2491 2468 Fragments arranged by size: 2491 2468 936 729 186 83 53 AccI GT'mk_AC Cuts at: 1937 3273 3817 4041 4289 1937 Size: 1336 544 224 248 4594 Fragments arranged by size: 4594 1336 544 248 224 Acc65I G'GTAC_C Cuts at: 4267 4267 Size: 6946 AclI AA'CG_TT Cuts at: 1563 5073 5446 1563 Size: 3510 373 3063 Fragments arranged by size: 3510 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3872 4238 5009 6393 6661 1632 Size: 2240 366 771 1384 268 1917 Fragments arranged by size: 2240 1917 1384 771 366 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6749 AflIII A'CryG_T Cuts at: 1203 2481 3787 5845 6822 1203 Size: 1278 1306 2058 977 1327 Fragments arranged by size: 2058 1327 1306 1278 977 AgeI A'CCGG_T Cuts at: 5777 5777 Size: 6946 AhdI GACnn_n'nnGTC Cuts at: 5675 5675 Size: 6946 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6946 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3399 1184 Size: 2215 4731 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3479 3511 3479 Size: 32 6914 AlwNI CAG_nnn'CTG Cuts at: 2184 2420 2440 2983 3950 4096 4105 4140 4689 Size: 236 20 543 967 146 9 35 549 Cuts at: 4689 6261 2184 Size: 1572 2869 Fragments arranged by size: 2869 1572 967 549 543 236 146 35 20 9 ApaI G_GGCC'C Cuts at: 3998 4216 3998 Size: 218 6728 ApaLI G'TGCA_C Cuts at: 3332 5003 6159 3332 Size: 1671 1156 4119 Fragments arranged by size: 4119 1671 1156 ApoI r'AATT_y Cuts at: 1493 3099 3727 4255 4481 4633 6742 1493 Size: 1606 628 528 226 152 2109 1697 Fragments arranged by size: 2109 1697 1606 628 528 226 152 AseI AT'TA_AT Cuts at: 160 1032 5500 160 Size: 872 4468 1606 Fragments arranged by size: 4468 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6946 AvaI C'yCGr_G Cuts at: 1870 1943 2745 4068 4271 1870 Size: 73 802 1323 203 4545 Fragments arranged by size: 4545 1323 802 203 73 BamHI G'GATC_C Cuts at: 4276 4643 4276 Size: 367 6579 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3146 3473 4267 5722 618 Size: 325 217 627 1359 327 794 1455 1842 Fragments arranged by size: 1842 1455 1359 794 627 327 325 217 BanII G_rGCy'C Cuts at: 1391 2174 2811 3709 3998 4152 4216 4265 1391 Size: 783 637 898 289 154 64 49 4072 Fragments arranged by size: 4072 898 783 637 289 154 64 49 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2265 928 Size: 971 366 5609 Fragments arranged by size: 5609 971 366 BbvCI CC'TCA_GC Cuts at: 2310 3798 2310 Size: 1488 5458 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2212 2246 3242 3276 5137 5171 1903 Size: 34 275 34 996 34 1861 34 3678 Fragments arranged by size: 3678 1861 996 275 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4838 6048 4838 Size: 1210 5736 BclI T'GATC_A Cuts at: 1548 1548 Size: 6946 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3916 3993 4230 Size: 107 122 71 631 605 2244 77 237 Cuts at: 4230 5557 136 Size: 1327 1525 Fragments arranged by size: 2244 1525 1327 631 605 237 122 107 77 71 BglII A'GATC_T Cuts at: 2898 6941 2898 Size: 4043 2903 BlpI GC'TnA_GC Cuts at: 4363 4363 Size: 6946 Bme1580I G_kGCm'C Cuts at: 1790 3336 3478 3564 3998 4216 5007 6163 1790 Size: 1546 142 86 434 218 791 1156 2573 Fragments arranged by size: 2573 1546 1156 791 434 218 142 86 BmgBI CAC'GTC Cuts at: 1318 2241 2431 3574 1318 Size: 923 190 1143 4690 Fragments arranged by size: 4690 1143 923 190 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3487 4677 5635 Size: 416 53 83 399 24 2070 1190 958 Cuts at: 5635 442 Size: 1753 Fragments arranged by size: 2070 1753 1190 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3366 1056 Size: 2310 4636 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2293 2486 2528 2543 3731 4082 5606 1239 Size: 1054 193 42 15 1188 351 1524 2579 Fragments arranged by size: 2579 1524 1188 1054 351 193 42 15 Bpu10I CC'TnA_GC Cuts at: 2310 2398 3798 3870 2310 Size: 88 1400 72 5386 Fragments arranged by size: 5386 1400 88 72 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3062 3581 3949 5071 5936 6234 3062 Size: 519 368 1122 865 298 3774 Fragments arranged by size: 3774 1122 865 519 368 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2309 5609 882 Size: 617 810 3300 2219 Fragments arranged by size: 3300 2219 810 617 BsaAI yAC'GTr Cuts at: 493 1204 2484 3331 4737 493 Size: 711 1280 847 1406 2702 Fragments arranged by size: 2702 1406 1280 847 711 BsaBI GATnn'nnATC Cuts at: 6940 6940 Size: 6946 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2262 2460 4753 5135 Size: 53 83 186 936 729 198 2293 382 Cuts at: 5135 5770 275 Size: 635 1451 Fragments arranged by size: 2293 1451 936 729 635 382 198 186 83 53 BsaWI w'CCGG_w Cuts at: 2727 5379 5777 6051 6198 6609 2727 Size: 2652 398 274 147 411 3064 Fragments arranged by size: 3064 2652 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2009 2039 1178 Size: 30 165 30 606 30 6085 Fragments arranged by size: 6085 606 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2023 2327 2540 1186 Size: 837 304 213 5592 Fragments arranged by size: 5592 837 304 213 BseYI C'CCAG_C Cuts at: 2167 2491 2628 3313 3479 3644 4105 6149 6645 Size: 324 137 685 166 165 461 2044 496 Cuts at: 6645 2167 Size: 2468 Fragments arranged by size: 2468 2044 685 496 461 324 166 165 137 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2346 2453 2907 2912 3041 3272 3527 Size: 573 610 107 454 5 129 231 255 Cuts at: 3527 3689 3815 3834 1163 Size: 162 126 19 4275 Fragments arranged by size: 4275 610 573 454 255 231 162 129 126 107 19 5 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2102 4396 5157 6185 1540 Size: 275 186 101 2294 761 1028 2301 Fragments arranged by size: 2301 2294 1028 761 275 186 101 BsiHKAI G_wGCw'C Cuts at: 2760 3336 3876 5007 5092 6163 2760 Size: 576 540 1131 85 1071 3543 Fragments arranged by size: 3543 1131 1071 576 540 85 BsmI GAATG_Cn' Cuts at: 4457 4550 4457 Size: 93 6853 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2174 2760 2811 3336 3478 3564 3709 Size: 399 384 586 51 525 142 86 145 Cuts at: 3709 3876 3998 4152 4216 4265 5007 5092 6163 Size: 167 122 154 64 49 742 85 1071 Cuts at: 6163 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 586 525 399 384 167 154 145 142 122 86 85 64 51 49 BspEI T'CCGG_A Cuts at: 2727 6609 2727 Size: 3882 3064 BspHI T'CATG_A Cuts at: 2476 4835 6565 2476 Size: 2359 1730 2857 Fragments arranged by size: 2857 2359 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2727 2799 2946 3461 4090 4301 844 Size: 1883 72 147 515 629 211 3489 Fragments arranged by size: 3489 1883 629 515 211 147 72 BsrBI CCG'CTC Cuts at: 1394 1823 4833 1394 Size: 429 3010 3507 Fragments arranged by size: 3507 3010 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3953 5441 5615 65 Size: 1183 2705 1488 174 1396 Fragments arranged by size: 2705 1488 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2278 3033 3793 5590 5777 1700 Size: 110 468 755 760 1797 187 2869 Fragments arranged by size: 2869 1797 760 755 468 187 110 BsrGI T'GTAC_A Cuts at: 96 4185 96 Size: 4089 2857 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6946 BssSI C'ACGA_G Cuts at: 5006 6018 5006 Size: 1012 5934 BstAPI GCAn_nnn'nTGC Cuts at: 794 2440 794 Size: 1646 5300 BstBI TT'CG_AA Cuts at: 5750 5750 Size: 6946 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6946 BstXI CCAn_nnnn'nTGG Cuts at: 4036 4036 Size: 6946 BstYI r'GATC_y Cuts at: 1972 2898 4276 4643 5028 5045 6497 6583 6595 Size: 926 1378 367 385 17 1452 86 12 Cuts at: 6595 6941 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1378 926 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3807 4050 3807 Size: 243 6703 BtgI C'CryG_G Cuts at: 513 1065 2096 2190 513 Size: 552 1031 94 5269 Fragments arranged by size: 5269 1031 552 94 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2209 3713 487 Size: 683 56 983 1504 3720 Fragments arranged by size: 3720 1504 983 683 56 BtsI GCAGTG_nn' Cuts at: 2218 2847 2924 3015 3515 3926 4462 5256 5276 Size: 629 77 91 500 411 536 794 20 Cuts at: 5276 2218 Size: 3888 Fragments arranged by size: 3888 794 629 536 500 411 91 77 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6946 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6911 DraI TTT'AAA Cuts at: 3118 4250 3118 Size: 1132 5814 DraIII CAC_nnn'GTG Cuts at: 3064 3403 3521 3064 Size: 339 118 6489 Fragments arranged by size: 6489 339 118 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5953 809 Size: 507 4637 1802 Fragments arranged by size: 4637 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2215 2253 3015 Size: 54 1425 50 275 18 385 38 762 Cuts at: 3015 3031 3512 4179 4231 4393 5282 6911 8 Size: 16 481 667 52 162 889 1629 43 Fragments arranged by size: 1629 1425 889 762 667 481 385 275 162 54 52 50 43 38 18 16 EagI C'GGCC_G Cuts at: 1537 1812 4393 1537 Size: 275 2581 4090 Fragments arranged by size: 4090 2581 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2553 3083 4876 1353 Size: 1200 530 1793 3423 Fragments arranged by size: 3423 1793 1200 530 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2159 5533 5907 6053 387 Size: 1064 138 570 3374 374 146 1280 Fragments arranged by size: 3374 1280 1064 570 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2293 2486 2528 2543 3731 3872 4082 Size: 393 661 193 42 15 1188 141 210 Cuts at: 4082 4238 5009 5606 6393 6661 1239 Size: 156 771 597 787 268 1524 Fragments arranged by size: 1524 1188 787 771 661 597 393 268 210 193 156 141 42 15 EcoO109I rG'GnC_Cy Cuts at: 2772 3307 3487 3803 3994 3995 4212 4213 2772 Size: 535 180 316 191 1 217 1 5505 Fragments arranged by size: 5505 535 316 217 191 180 1 1 EcoRI G'AATT_C Cuts at: 3727 4255 4633 3727 Size: 528 378 6040 Fragments arranged by size: 6040 528 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3622 3622 Size: 6946 FseI GG_CCGG'CC Cuts at: 2282 3037 2282 Size: 755 6191 FspI TGC'GCA Cuts at: 2651 5452 2651 Size: 2801 4145 HaeII r_GCGC'y Cuts at: 1678 2085 2108 2184 2252 2983 6093 1678 Size: 407 23 76 68 731 3110 2531 Fragments arranged by size: 3110 2531 731 407 76 68 23 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2693 2725 2903 2935 5587 Size: 32 135 32 1121 32 178 32 2652 Cuts at: 5587 5619 5661 5693 1373 Size: 32 42 32 2626 Fragments arranged by size: 2652 2626 1121 178 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3818 4042 4290 4536 669 Size: 988 281 1880 224 248 246 3079 Fragments arranged by size: 3079 1880 988 281 248 246 224 HindIII A'AGCT_T Cuts at: 748 748 Size: 6946 HpaI GTT'AAC Cuts at: 4536 4536 Size: 6946 KpnI G_GTAC'C Cuts at: 4271 4271 Size: 6946 MfeI C'AATT_G Cuts at: 4545 4545 Size: 6946 MluI A'CGCG_T Cuts at: 6822 6822 Size: 6946 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2145 6060 6244 1305 Size: 840 3915 184 2007 Fragments arranged by size: 3915 2007 840 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3017 3514 4181 4233 10 Size: 54 1425 343 1185 497 667 52 2723 Fragments arranged by size: 2723 1425 1185 667 497 343 54 52 MslI CAynn'nnrTG Cuts at: 518 1166 4904 5263 5422 518 Size: 648 3738 359 159 2042 Fragments arranged by size: 3738 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2022 2098 2128 3422 3644 3653 4036 4060 Size: 416 76 30 1294 222 9 383 24 Cuts at: 4060 4362 5039 6187 6432 1606 Size: 302 677 1148 245 2120 Fragments arranged by size: 2120 1294 1148 677 416 383 302 245 222 76 30 24 9 NaeI GCC'GGC Cuts at: 2280 3035 3795 2280 Size: 755 760 5431 Fragments arranged by size: 5431 760 755 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6394 NdeI CA'TA_TG Cuts at: 387 387 Size: 6946 NgoMIV G'CCGG_C Cuts at: 2278 3033 3793 2278 Size: 755 760 5431 Fragments arranged by size: 5431 760 755 NheI G'CTAG_C Cuts at: 1052 3362 1052 Size: 2310 4636 NotI GC'GGCC_GC Cuts at: 4393 4393 Size: 6946 NspI r_CATG'y Cuts at: 1132 1229 3791 4304 5849 1132 Size: 97 2562 513 1545 2229 Fragments arranged by size: 2562 2229 1545 513 97 PasI CC'CwG_GG Cuts at: 1348 3743 1348 Size: 2395 4551 PciI A'CATG_T Cuts at: 3787 5845 3787 Size: 2058 4888 PflMI CCAn_nnn'nTGG Cuts at: 1108 2420 2874 1108 Size: 1312 454 5180 Fragments arranged by size: 5180 1312 454 PfoI T'CCnGG_A Cuts at: 2967 3627 2967 Size: 660 6286 PmeI GTTT'AAAC Cuts at: 4250 4250 Size: 6946 PmlI CAC'GTG Cuts at: 2484 2484 Size: 6946 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5015 5047 6528 6560 5015 Size: 32 1481 32 5401 Fragments arranged by size: 5401 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2772 3307 3487 3803 2772 Size: 535 180 316 5915 Fragments arranged by size: 5915 535 316 180 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6946 PsiI TTA'TAA Cuts at: 4516 4516 Size: 6946 PspOMI G'GGCC_C Cuts at: 3994 4212 3994 Size: 218 6728 PstI C_TGCA'G Cuts at: 830 2142 2336 2796 3643 4101 4243 4298 830 Size: 1312 194 460 847 458 142 55 3478 Fragments arranged by size: 3478 1312 847 460 458 194 142 55 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6946 PvuII CAG'CTG Cuts at: 3644 3653 4036 4060 3644 Size: 9 383 24 6530 Fragments arranged by size: 6530 383 24 9 SacII CC_GC'GG Cuts at: 2099 2099 Size: 6946 SalI G'TCGA_C Cuts at: 1936 3816 4040 4288 1936 Size: 1880 224 248 4594 Fragments arranged by size: 4594 1880 248 224 SbfI CC_TGCA'GG Cuts at: 4298 4298 Size: 6946 ScaI AGT'ACT Cuts at: 5194 6906 5194 Size: 1712 5234 SfcI C'TryA_G Cuts at: 826 1045 2138 2332 2792 3106 3639 4097 4239 Size: 219 1093 194 460 314 533 458 142 Cuts at: 4239 4294 5429 6110 6301 826 Size: 55 1135 681 191 1471 Fragments arranged by size: 1471 1135 1093 681 533 460 458 314 219 194 191 142 55 SfiI GGCCn_nnn'nGGCC Cuts at: 4230 4230 Size: 6946 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6946 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6946 SmaI CCC'GGG Cuts at: 1872 4273 1872 Size: 2401 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3077 3596 3928 5050 5951 6213 Size: 197 926 1134 519 332 1122 901 262 Cuts at: 6213 820 Size: 1553 Fragments arranged by size: 1553 1134 1122 926 901 519 332 262 197 SnaBI TAC'GTA Cuts at: 493 4737 493 Size: 4244 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6946 SphI G_CATG'C Cuts at: 1132 4304 1132 Size: 3172 3774 SspI AAT'ATT Cuts at: 5 52 4870 5 Size: 47 4818 2081 Fragments arranged by size: 4818 2081 47 StuI AGG'CCT Cuts at: 1517 2381 1517 Size: 864 6082 StyI C'CwwG_G Cuts at: 513 1065 3294 3300 3501 4385 513 Size: 552 2229 6 201 884 3074 Fragments arranged by size: 3074 2229 884 552 201 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4973 4990 5143 5328 5773 325 Size: 1255 3393 17 153 185 445 1498 Fragments arranged by size: 3393 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4185 5192 6904 Size: 274 80 33 51 1443 2208 1007 1712 Cuts at: 6904 96 Size: 138 Fragments arranged by size: 2208 1712 1443 1007 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3130 3162 6906 6938 3130 Size: 32 3744 32 3138 Fragments arranged by size: 3744 3138 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6946 XbaI T'CTAG_A Cuts at: 4282 4282 Size: 6946 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2902 3400 2902 Size: 498 6448 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6946 XmaI C'CCGG_G Cuts at: 1870 4271 1870 Size: 2401 4545 XmnI GAAnn'nnTTC Cuts at: 5075 5075 Size: 6946 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2263 4754 276 Size: 53 83 186 936 729 2491 2468 Fragments arranged by size: 2491 2468 936 729 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoRV FspAI KasI NarI NruI NsiI PacI PspXI PsrI RsrII SacI SanDI SapI SexAI SfoI SrfI SwaI