Restriction map for
pFN21AB4140
(Linear) (Six-base) MAPSORT of: KIBB4140.seq Check: 7545 from: 1 to: 2241
KIBB4140.orf
With 182 enzymes: SgfI *
July 18, 2007 13:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1456 2085 2241
Size: 1456 629 156
Fragments arranged by size:
1456 629 156
AatII G_ACGT'C
Cuts at: 0 260 2241
Size: 260 1981
AccI GT'mk_AC
Cuts at: 0 1268 1812 2036 2241
Size: 1268 544 224 205
Fragments arranged by size:
1268 544 224 205
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1867 2233 2241
Size: 1867 366 8
Fragments arranged by size:
1867 366 8
AflIII A'CryG_T
Cuts at: 0 476 1782 2241
Size: 476 1306 459
Fragments arranged by size:
1306 476 459
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1394 2241
Size: 1394 847
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1474 1506 2241
Size: 1474 32 735
Fragments arranged by size:
1474 735 32
AlwNI CAG_nnn'CTG
Cuts at: 0 179 415 435 978 1945 2091 2100 2135
Size: 179 236 20 543 967 146 9 35
Cuts at: 2135 2241
Size: 106
Fragments arranged by size:
967 543 236 179 146 106 35 20
9
ApaI G_GGCC'C
Cuts at: 0 1993 2211 2241
Size: 1993 218 30
Fragments arranged by size:
1993 218 30
ApaLI G'TGCA_C
Cuts at: 0 1327 2241
Size: 1327 914
ApoI r'AATT_y
Cuts at: 0 1094 1722 2241
Size: 1094 628 519
Fragments arranged by size:
1094 628 519
AvaI C'yCGr_G
Cuts at: 0 740 2063 2241
Size: 740 1323 178
Fragments arranged by size:
1323 740 178
BanI G'GyrC_C
Cuts at: 0 1141 1468 2241
Size: 1141 327 773
Fragments arranged by size:
1141 773 327
BanII G_rGCy'C
Cuts at: 0 169 806 1704 1993 2147 2211 2241
Size: 169 637 898 289 154 64 30
Fragments arranged by size:
898 637 289 169 154 64 30
BbsI GAAGACnn'nnnn_
Cuts at: 0 260 2241
Size: 260 1981
BbvCI CC'TCA_GC
Cuts at: 0 305 1793 2241
Size: 305 1488 448
Fragments arranged by size:
1488 448 305
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 207 241 1237 1271 2241
Size: 207 34 996 34 970
Fragments arranged by size:
996 970 207 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1911 1988 2225 2241
Size: 1911 77 237 16
Fragments arranged by size:
1911 237 77 16
BglII A'GATC_T
Cuts at: 0 893 2241
Size: 893 1348
Bme1580I G_kGCm'C
Cuts at: 0 1331 1473 1559 1993 2211 2241
Size: 1331 142 86 434 218 30
Fragments arranged by size:
1331 434 218 142 86 30
BmgBI CAC'GTC
Cuts at: 0 236 426 1569 2241
Size: 236 190 1143 672
Fragments arranged by size:
1143 672 236 190
BmrI ACTGGGnnnn_n'
Cuts at: 0 1482 2241
Size: 1482 759
BmtI G_CTAG'C
Cuts at: 0 1361 2241
Size: 1361 880
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 288 481 523 538 1726 2077 2241
Size: 288 193 42 15 1188 351 164
Fragments arranged by size:
1188 351 288 193 164 42 15
Bpu10I CC'TnA_GC
Cuts at: 0 305 393 1793 1865 2241
Size: 305 88 1400 72 376
Fragments arranged by size:
1400 376 305 88 72
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1057 1576 1944 2241
Size: 1057 519 368 297
Fragments arranged by size:
1057 519 368 297
BsaI GGTCTCn'nnnn_
Cuts at: 0 304 2241
Size: 304 1937
BsaAI yAC'GTr
Cuts at: 0 479 1326 2241
Size: 479 847 915
Fragments arranged by size:
915 847 479
BsaHI Gr'CG_yC
Cuts at: 0 257 455 2241
Size: 257 198 1786
Fragments arranged by size:
1786 257 198
BsaWI w'CCGG_w
Cuts at: 0 722 2241
Size: 722 1519
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 4 34 2241
Size: 4 30 2207
Fragments arranged by size:
2207 30 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 18 322 535 2241
Size: 18 304 213 1706
Fragments arranged by size:
1706 304 213 18
BseYI C'CCAG_C
Cuts at: 0 162 486 623 1308 1474 1639 2100 2241
Size: 162 324 137 685 166 165 461 141
Fragments arranged by size:
685 461 324 166 165 162 141 137
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 341 448 902 907 1036 1267 1522 1684
Size: 341 107 454 5 129 231 255 162
Cuts at: 1684 1810 1829 2241
Size: 126 19 412
Fragments arranged by size:
454 412 341 255 231 162 129 126
107 19 5
BsiEI CG_ry'CG
Cuts at: 0 97 2241
Size: 97 2144
BsiHKAI G_wGCw'C
Cuts at: 0 755 1331 1871 2241
Size: 755 576 540 370
Fragments arranged by size:
755 576 540 370
Bsp1286I G_dGCh'C
Cuts at: 0 169 755 806 1331 1473 1559 1704 1871
Size: 169 586 51 525 142 86 145 167
Cuts at: 1871 1993 2147 2211 2241
Size: 122 154 64 30
Fragments arranged by size:
586 525 169 167 154 145 142 122
86 64 51 30
BspEI T'CCGG_A
Cuts at: 0 722 2241
Size: 722 1519
BspHI T'CATG_A
Cuts at: 0 471 2241
Size: 471 1770
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 722 794 941 1456 2085 2241
Size: 722 72 147 515 629 156
Fragments arranged by size:
722 629 515 156 147 72
BsrDI GCAATG_nn'
Cuts at: 0 1948 2241
Size: 1948 293
BsrFI r'CCGG_y
Cuts at: 0 273 1028 1788 2241
Size: 273 755 760 453
Fragments arranged by size:
760 755 453 273
BsrGI T'GTAC_A
Cuts at: 0 2180 2241
Size: 2180 61
BstAPI GCAn_nnn'nTGC
Cuts at: 0 435 2241
Size: 435 1806
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2031 2241
Size: 2031 210
BstYI r'GATC_y
Cuts at: 0 893 2241
Size: 893 1348
Bsu36I CC'TnA_GG
Cuts at: 0 1802 2045 2241
Size: 1802 243 196
Fragments arranged by size:
1802 243 196
BtgI C'CryG_G
Cuts at: 0 91 185 2241
Size: 91 94 2056
Fragments arranged by size:
2056 94 91
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 204 1708 2241
Size: 204 1504 533
Fragments arranged by size:
1504 533 204
BtsI GCAGTG_nn'
Cuts at: 0 213 842 919 1010 1510 1921 2241
Size: 213 629 77 91 500 411 320
Fragments arranged by size:
629 500 411 320 213 91 77
DraI TTT'AAA
Cuts at: 0 1113 2241
Size: 1113 1128
DraIII CAC_nnn'GTG
Cuts at: 0 1059 1398 1516 2241
Size: 1059 339 118 725
Fragments arranged by size:
1059 725 339 118
EaeI y'GGCC_r
Cuts at: 0 210 248 1010 1026 1507 2174 2226 2241
Size: 210 38 762 16 481 667 52 15
Fragments arranged by size:
762 667 481 210 52 38 16 15
EarI CTCTTCn'nnn_
Cuts at: 0 548 1078 2241
Size: 548 530 1163
Fragments arranged by size:
1163 548 530
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 154 2241
Size: 154 2087
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 288 481 523 538 1726 1867 2077 2233
Size: 288 193 42 15 1188 141 210 156
Cuts at: 2233 2241
Size: 8
Fragments arranged by size:
1188 288 210 193 156 141 42 15
8
EcoO109I rG'GnC_Cy
Cuts at: 0 767 1302 1482 1798 1989 1990 2207 2208
Size: 767 535 180 316 191 1 217 1
Cuts at: 2208 2241
Size: 33
Fragments arranged by size:
767 535 316 217 191 180 33 1
1
EcoRI G'AATT_C
Cuts at: 0 1722 2241
Size: 1722 519
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1617 2241
Size: 1617 624
FseI GG_CCGG'CC
Cuts at: 0 277 1032 2241
Size: 277 755 1209
Fragments arranged by size:
1209 755 277
FspI TGC'GCA
Cuts at: 0 646 2241
Size: 646 1595
HaeII r_GCGC'y
Cuts at: 0 80 103 179 247 978 2241
Size: 80 23 76 68 731 1263
Fragments arranged by size:
1263 731 80 76 68 23
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 688 720 898 930 2241
Size: 688 32 178 32 1311
Fragments arranged by size:
1311 688 178 32 32
HincII GTy'rAC
Cuts at: 0 1813 2037 2241
Size: 1813 224 204
Fragments arranged by size:
1813 224 204
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 140 2241
Size: 140 2101
MscI TGG'CCA
Cuts at: 0 1012 1509 2176 2228 2241
Size: 1012 497 667 52 13
Fragments arranged by size:
1012 667 497 52 13
MspA1I CmG'CkG
Cuts at: 0 17 93 123 1417 1639 1648 2031 2055
Size: 17 76 30 1294 222 9 383 24
Cuts at: 2055 2241
Size: 186
Fragments arranged by size:
1294 383 222 186 76 30 24 17
9
NaeI GCC'GGC
Cuts at: 0 275 1030 1790 2241
Size: 275 755 760 451
Fragments arranged by size:
760 755 451 275
NgoMIV G'CCGG_C
Cuts at: 0 273 1028 1788 2241
Size: 273 755 760 453
Fragments arranged by size:
760 755 453 273
NheI G'CTAG_C
Cuts at: 0 1357 2241
Size: 1357 884
NspI r_CATG'y
Cuts at: 0 1786 2241
Size: 1786 455
PasI CC'CwG_GG
Cuts at: 0 1738 2241
Size: 1738 503
PciI A'CATG_T
Cuts at: 0 1782 2241
Size: 1782 459
PflMI CCAn_nnn'nTGG
Cuts at: 0 415 869 2241
Size: 415 454 1372
Fragments arranged by size:
1372 454 415
PfoI T'CCnGG_A
Cuts at: 0 962 1622 2241
Size: 962 660 619
Fragments arranged by size:
962 660 619
PmlI CAC'GTG
Cuts at: 0 479 2241
Size: 479 1762
PpuMI rG'GwC_Cy
Cuts at: 0 767 1302 1482 1798 2241
Size: 767 535 180 316 443
Fragments arranged by size:
767 535 443 316 180
PspOMI G'GGCC_C
Cuts at: 0 1989 2207 2241
Size: 1989 218 34
Fragments arranged by size:
1989 218 34
PstI C_TGCA'G
Cuts at: 0 137 331 791 1638 2096 2238 2241
Size: 137 194 460 847 458 142 3
Fragments arranged by size:
847 460 458 194 142 137 3
PvuII CAG'CTG
Cuts at: 0 1639 1648 2031 2055 2241
Size: 1639 9 383 24 186
Fragments arranged by size:
1639 383 186 24 9
SacII CC_GC'GG
Cuts at: 0 94 2241
Size: 94 2147
SalI G'TCGA_C
Cuts at: 0 1811 2035 2241
Size: 1811 224 206
Fragments arranged by size:
1811 224 206
SfcI C'TryA_G
Cuts at: 0 133 327 787 1101 1634 2092 2234 2241
Size: 133 194 460 314 533 458 142 7
Fragments arranged by size:
533 460 458 314 194 142 133 7
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2225 2241
Size: 2225 16
SmlI C'TyrA_G
Cuts at: 0 1072 1591 1923 2241
Size: 1072 519 332 318
Fragments arranged by size:
1072 519 332 318
StuI AGG'CCT
Cuts at: 0 376 2241
Size: 376 1865
StyI C'CwwG_G
Cuts at: 0 1289 1295 1496 2241
Size: 1289 6 201 745
Fragments arranged by size:
1289 745 201 6
TatI w'GTAC_w
Cuts at: 0 2180 2241
Size: 2180 61
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1125 1157 2241
Size: 1125 32 1084
Fragments arranged by size:
1125 1084 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 897 1395 2241
Size: 897 498 846
Fragments arranged by size:
897 846 498
ZraI GAC'GTC
Cuts at: 0 258 2241
Size: 258 1983
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AlfI AloI AlwNI
ApaI ApaLI ApoI AvaI BanI BanII BbsI BbvCI
BcgI BglI BglII Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrDI BsrFI BsrGI BstAPI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII EaeI EarI EciI Eco57MI
EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII
MmeI MscI MspA1I NaeI NgoMIV NheI NspI PasI
PciI PflMI PfoI PmlI PpuMI PspOMI PstI PvuII
SacII SalI SfcI SfiI SmlI StuI StyI TatI
TstI XcmI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AgeI AhdI AleI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BciVI BclI
BfrBI BlpI BplI BsaBI BsiWI BsmI BsmBI BsrBI
BssHII BssSI BstBI BstEII BstZ17I ClaI CspCI DrdI
EagI EcoICRI EcoNI EcoRV FspAI HindIII HpaI KasI
KpnI MfeI MluI MslI NarI NcoI NdeI NotI
NruI NsiI PacI PmeI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SanDI SapI SbfI ScaI
SexAI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI SwaI TaqII Tth111I XbaI XhoI XmaI
XmnI