Restriction map for
pFN21AB4051
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# NO CUT ENZYMES ( 171 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflIII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AvaII
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbsI BbvCI
BcgI BclI BcnI BglI BlnI
BpmI BpuAI BsaAI BsaHI BsaI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Cfr10I Cfr13I ClaI CpoI DraII
DraIII DrdI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoO109I
EcoO65I EcoRI EcoRV EcoT14I FauI
FbaI FseI FspI HapII HgaI
Hin1I HincII HindII HindIII HpaI
HpaII KasI KpnI Ksp632I KspI
MaeII MfeI MluI MluNI MroI
MscI MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SapI
Sau96I SbfI ScaI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AcsI 113 AAATTC [GA]^AATT[CT]
AflII 493 CTTAAG C^TTAAG
AlwNI 593 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 946 CCTCAGG CC^T[ACGT]AGG
ApoI 113 AAATTC [GA]^AATT[CT]
BciVI 531 GGATAC GTATCC(6/5)
BfrI 493 CTTAAG C^TTAAG
BglII 489 AGATCT A^GATCT
BlpI 441 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 441 GCTGAGC GC^T[ACGT]AGC
BsaBI 732 GATTATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 371 GAGGAG GAGGAG(10/8)
BsgI 92 CTGCAC GTGCAG(16/14)
BsmAI 778 GAGAC GTCTC(1/5)
BsmFI 252 GTCCC GGGAC(10/14)
BsmI 214 GAATGC GAATGC(1/-1)
BsrDI 745 GCAATG GCAATG(2/0)
BsrI 536 CCAGT ACTGG(1/-1)
BstXI 536 CCAGTGACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 489 AGATCT [GA]^GATC[CT]
Bsu36I 946 CCTCAGG CC^T[ACGT]AGG
CelII 441 GCTGAGC GC^T[ACGT]AGC
CfoI 332 GCGC GCG^C
DraI 1000 TTTAAA TTT^AAA
DsaI 255 CCACGG C^C[GA][CT]GG
Eco81I 946 CCTCAGG CC^T[ACGT]AGG
EcoNI 222 CCTACAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 216 ATGCAT ATGCA^T
HaeII 331 AGCGCC [GA]GCGC^[CT]
HaeIII 43 GGCC GG^CC
HhaI 332 GCGC GCG^C
HinP1I 332 GCGC G^CGC
HinfI 201 GATTC G^A[ACGT]TC
HphI 3 GGTGA GGTGA(8/7)
MamI 732 GATTATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 489 AGATCT [GA]^GATC[CT]
MwoI 94 GCACGGGTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NsiI 216 ATGCAT ATGCA^T
NspI 652 GCATGT [GA]CATG^[CT]
SexAI 523 ACCAGGT A^CC[AT]GGT
SmlI 493 CTTAAG C^T[CT][GA]AG
TaqI 117 TCGA T^CGA
TfiI 201 GATTC G^A[AT]TC
TthHB8I 117 TCGA T^CGA
XhoII 489 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 16 enzymes) #
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AfaI 21 GTAC GT^AC
988 GTAC
BbvI 1010 GCAGC GCAGC(8/12)
937 GCTGC
BfaI 145 CTAG C^TAG
973 CTAG
BmyI 164 GGGCAC G[AGT]GC[ACT]^C
449 GTGCCC
Bsp1286I 164 GGGCAC G[AGT]GC[ACT]^C
449 GTGCCC
Cac8I 11 GCCAGC GC[ACGT]^[ACGT]GC
933 GCTGGC
Fnu4HI 937 GCTGC GC^[ACGT]GC
1010 GCAGC
ItaI 937 GCTGC GC^[ACGT]GC
1010 GCAGC
MaeI 145 CTAG C^TAG
973 CTAG
MslI 190 CATCCAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
876 CATTCCTGTG
MspA1I 439 CAGCTG C[AC]G^C[GT]G
1011 CAGCTG
NlaIV 546 GGAACC GG[ACGT]^[ACGT]CC
675 GGCTCC
PvuII 439 CAGCTG CAG^CTG
1011 CAGCTG
RsaI 21 GTAC GT^AC
988 GTAC
SfaNI 961 GATGC GCATC(5/9)
218 GCATC
TseI 937 GCTGC G^C[AT]GC
1010 GCAGC
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# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AluI AlwI BsaJI BsiYI BslI
BssKI BstF5I BstNI DdeI DpnII
EcoRII FokI MaeIII MboI MboII
MnlI MseI MvaI NdeII NlaIII
Sau3AI ScrFI Tru9I Tsp45I Tsp509I
TspRI