Restriction map for
pFN21AB4019
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BanI BbeI BbsI BbvCI
BcgI BclI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaBI BsaHI BsaI BseAI BsiEI
BsiWI BsoBI BspDI BspEI BsrDI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstYI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV FbaI FseI FspI
HaeII HgaI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MaeI MamI MfeI MflI MluI
MluNI MroI MscI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmeI PpuMI PshAI PshBI
Psp1406I PstI PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SwaI TaqI Tth111I TthHB8I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AccII 698 CGCG CG^CG
AflIII 1016 ACATGT A^C[GA][CT]GT
AlwI 672 GATCC GGATC(4/5)
ApaI 1079 GGGCCC GGGCC^C
AspHI 651 GAGCAC G[AT]GC[AT]^C
BcnI 58 CCCGG CC^[GC]GG
BsaWI 866 TCCGGT [AT]^CCGG[AT]
BsgI 1048 GTGCAG GTGCAG(16/14)
BsiHKAI 651 GAGCAC G[AT]GC[AT]^C
BsmBI 708 GAGACG CGTCTC(1/5)
Bsp1407I 1178 TGTACA T^GTACA
BspHI 1040 TCATGA T^CATGA
BspLU11I 1016 ACATGT A^CATGT
BspMI 415 GCAGGT ACCTGC(4/8)
BsrBI 592 GAGCGG CCGCTC(-3/-3)
BsrGI 1178 TGTACA T^GTACA
BstUI 698 CGCG CG^CG
DraI 201 TTTAAA TTT^AAA
DsaI 954 CCATGG C^C[GA][CT]GG
EaeI 47 TGGCCG [CT]^GGCC[GA]
EarI 705 GAAGAG CTCTTC(1/4)
EcoNI 1249 CCTACAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 22 ATGCAT ATGCA^T
Ksp632I 705 GAAGAG CTCTTC(1/4)
MvnI 698 CGCG CG^CG
NciI 58 CCCGG CC^[GC]GG
NcoI 954 CCATGG C^CATGG
NsiI 22 ATGCAT ATGCA^T
PflMI 165 CCAGAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1160 GACTC GAGTC(4/5)
RcaI 1040 TCATGA T^CATGA
SfcI 1250 CTACAG C^T[GA][CT]AG
SmlI 643 CTCAAG C^T[CT][GA]AG
SspBI 1178 TGTACA T^GTACA
StuI 509 AGGCCT AGG^CCT
Van91I 165 CCAGAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcsI 714 GAATTT [GA]^AATT[CT]
889 AAATTT
AfaI 870 GTAC GT^AC
1179 GTAC
ApoI 714 GAATTT [GA]^AATT[CT]
889 AAATTT
AvaII 95 GGACC G^G[AT]CC
563 GGACC
BanII 281 GAGCCC G[GA]GC[CT]^C
1079 GGGCCC
BbrPI 532 CACGTG CAC^GTG
654 CACGTG
BsaAI 532 CACGTG [CT]AC^GT[GA]
654 CACGTG
BsmFI 94 GGGAC GGGAC(10/14)
66 GTCCC
BsmI 24 GCATTC GAATGC(1/-1)
438 GCATTC
CfoI 181 GCGC GCG^C
697 GCGC
DpnII 468 GATC ^GATC
672 GATC
EcoT14I 954 CCATGG C^C[AT][AT]GG
1074 CCAAGG
FauI 110 GCGGG CCCGC(4/6)
352 GCGGG
HhaI 181 GCGC GCG^C
697 GCGC
HinP1I 181 GCGC G^CGC
697 GCGC
HphI 666 GGTGA GGTGA(8/7)
524 TCACC
MboI 468 GATC ^GATC
672 GATC
NdeII 468 GATC ^GATC
672 GATC
PmaCI 532 CACGTG CAC^GTG
654 CACGTG
PmlI 532 CACGTG CAC^GTG
654 CACGTG
PvuII 310 CAGCTG CAG^CTG
926 CAGCTG
RsaI 870 GTAC GT^AC
1179 GTAC
Sau3AI 468 GATC ^GATC
672 GATC
SspI 749 AATATT AAT^ATT
907 AATATT
StyI 954 CCATGG C^C[AT][AT]GG
1074 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AluI BbvI BciVI BmyI
BpmI BsaJI BseRI BsiYI BslI
BsmAI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DraII
EcoO109I EcoRII Fnu4HI FokI HaeIII
HapII HinfI HpaII ItaI MaeII
MaeIII MboII MnlI MseI MslI
MspA1I MspI MvaI MwoI NlaIII
NlaIV NspI Sau96I ScrFI SfaNI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI