(Circular) (Six-base) MAPSORT of: pFN21AB4019.seq Check: 1402 from: 1 to: 5995 pFN21AB4019 5995 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3805 278 Size: 53 83 186 936 2269 2468 Fragments arranged by size: 2468 2269 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3338 1937 Size: 1401 4594 Acc65I G'GTAC_C Cuts at: 3316 3316 Size: 5995 AclI AA'CG_TT Cuts at: 1563 3294 4122 4495 1563 Size: 1731 828 373 3063 Fragments arranged by size: 3063 1731 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4058 5442 5710 1632 Size: 2426 1384 268 1917 Fragments arranged by size: 2426 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5798 AflIII A'CryG_T Cuts at: 1203 3021 4894 5871 1203 Size: 1818 1873 977 1327 Fragments arranged by size: 1873 1818 1327 977 AgeI A'CCGG_T Cuts at: 4826 4826 Size: 5995 AhdI GACnn_n'nnGTC Cuts at: 4724 4724 Size: 5995 AleI CACnn'nnGTG Cuts at: 1166 2537 1166 Size: 1371 4624 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2052 1184 Size: 868 5127 AlwNI CAG_nnn'CTG Cuts at: 3738 5310 3738 Size: 1572 4423 ApaI G_GGCC'C Cuts at: 3088 3088 Size: 5995 ApaLI G'TGCA_C Cuts at: 4052 5208 4052 Size: 1156 4839 ApoI r'AATT_y Cuts at: 1493 2719 2894 3304 3530 3682 5791 1493 Size: 1226 175 410 226 152 2109 1697 Fragments arranged by size: 2109 1697 1226 410 226 175 152 AseI AT'TA_AT Cuts at: 160 1032 4549 160 Size: 872 3517 1606 Fragments arranged by size: 3517 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5995 AvaI C'yCGr_G Cuts at: 1870 1943 3320 1870 Size: 73 1377 4545 Fragments arranged by size: 4545 1377 73 BamHI G'GATC_C Cuts at: 3325 3692 3325 Size: 367 5628 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3316 4771 618 Size: 325 217 627 1529 1455 1842 Fragments arranged by size: 1842 1529 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2290 3088 3314 1391 Size: 899 798 226 4072 Fragments arranged by size: 4072 899 798 226 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5024 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4186 4220 1903 Size: 34 2249 34 3678 Fragments arranged by size: 3678 2249 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2288 3112 3146 3887 5097 2288 Size: 824 34 741 1210 3186 Fragments arranged by size: 3186 1210 824 741 34 BclI T'GATC_A Cuts at: 1548 1548 Size: 5995 BfrBI ATG'CAT Cuts at: 2029 2029 Size: 5995 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4606 136 Size: 107 122 71 631 605 2934 1525 Fragments arranged by size: 2934 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5990 5990 Size: 5995 BlpI GC'TnA_GC Cuts at: 3412 3412 Size: 5995 Bme1580I G_kGCm'C Cuts at: 1790 3088 4056 5212 1790 Size: 1298 968 1156 2573 Fragments arranged by size: 2573 1298 1156 968 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5995 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2781 3726 4684 Size: 416 53 83 399 24 1364 945 958 Cuts at: 4684 442 Size: 1753 Fragments arranged by size: 1753 1364 958 945 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5995 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2480 2480 Size: 5995 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2433 2519 3054 4655 1239 Size: 1194 86 535 1601 2579 Fragments arranged by size: 2579 1601 1194 535 86 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2633 4120 4985 5283 2633 Size: 1487 865 298 3345 Fragments arranged by size: 3345 1487 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4658 882 Size: 617 3159 2219 Fragments arranged by size: 3159 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2539 2661 3786 493 Size: 711 1335 122 1125 2702 Fragments arranged by size: 2702 1335 1125 711 122 BsaBI GATnn'nnATC Cuts at: 5989 5989 Size: 5995 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3802 4184 4819 275 Size: 53 83 186 936 2269 382 635 1451 Fragments arranged by size: 2269 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2871 4428 4826 5100 5247 5658 2871 Size: 1557 398 274 147 411 3208 Fragments arranged by size: 3208 1557 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3087 3117 1178 Size: 30 165 30 1684 30 4056 Fragments arranged by size: 4056 1684 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2298 2309 2819 1186 Size: 1112 11 510 4362 Fragments arranged by size: 4362 1112 510 11 BseYI C'CCAG_C Cuts at: 2039 2073 2313 5198 5694 2039 Size: 34 240 2885 496 2340 Fragments arranged by size: 2885 2340 496 240 34 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3074 1163 Size: 573 1338 4084 Fragments arranged by size: 4084 1338 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3445 4206 5234 1540 Size: 275 186 1444 761 1028 2301 Fragments arranged by size: 2301 1444 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2660 4056 4141 5212 2660 Size: 1396 85 1071 3443 Fragments arranged by size: 3443 1396 1071 85 BsmI GAATG_Cn' Cuts at: 2029 2443 3506 3599 2029 Size: 414 1063 93 4425 Fragments arranged by size: 4425 1063 414 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2707 2707 Size: 5995 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2290 2660 3088 3314 4056 4141 5212 Size: 399 500 370 428 226 742 85 1071 Cuts at: 5212 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 500 428 399 370 226 85 BspEI T'CCGG_A Cuts at: 5658 5658 Size: 5995 BspHI T'CATG_A Cuts at: 3045 3884 5614 3045 Size: 839 1730 3426 Fragments arranged by size: 3426 1730 839 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2411 3350 844 Size: 1567 939 3489 Fragments arranged by size: 3489 1567 939 BsrBI CCG'CTC Cuts at: 1394 1823 2599 3882 1394 Size: 429 776 1283 3507 Fragments arranged by size: 3507 1283 776 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4490 4664 65 Size: 1183 3242 174 1396 Fragments arranged by size: 3242 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4639 4826 1700 Size: 110 2829 187 2869 Fragments arranged by size: 2869 2829 187 110 BsrGI T'GTAC_A Cuts at: 96 3183 96 Size: 3087 2908 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5995 BssSI C'ACGA_G Cuts at: 4055 5067 4055 Size: 1012 4983 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5995 BstBI TT'CG_AA Cuts at: 4799 4799 Size: 5995 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5995 BstYI r'GATC_y Cuts at: 1972 3325 3692 4077 4094 5546 5632 5644 5990 Size: 1353 367 385 17 1452 86 12 346 Cuts at: 5990 1972 Size: 1977 Fragments arranged by size: 1977 1452 1353 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2959 513 Size: 552 939 955 3549 Fragments arranged by size: 3549 955 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5256 Fragments arranged by size: 5256 683 56 BtsI GCAGTG_nn' Cuts at: 2937 3277 3511 4305 4325 2937 Size: 340 234 794 20 4607 Fragments arranged by size: 4607 794 340 234 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5995 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2803 2838 529 Size: 35 2239 35 3686 Fragments arranged by size: 3686 2239 35 35 DraI TTT'AAA Cuts at: 2208 3299 2208 Size: 1091 4904 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5002 809 Size: 507 3686 1802 Fragments arranged by size: 3686 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2052 3442 4331 Size: 54 1425 50 275 18 222 1390 889 Cuts at: 4331 5960 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1390 889 275 222 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3442 1537 Size: 275 1630 4090 Fragments arranged by size: 4090 1630 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2705 3925 1353 Size: 1352 1220 3423 Fragments arranged by size: 3423 1352 1220 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2388 2722 4582 4956 5102 387 Size: 1064 138 799 334 1860 374 146 1280 Fragments arranged by size: 1860 1280 1064 799 374 334 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2433 2519 3054 4058 4655 5442 5710 Size: 393 801 86 535 1004 597 787 268 Cuts at: 5710 1239 Size: 1524 Fragments arranged by size: 1524 1004 801 787 597 535 393 268 86 EcoNI CCTnn'n_nnAGG Cuts at: 3258 3258 Size: 5995 EcoO109I rG'GnC_Cy Cuts at: 2360 3084 3263 2360 Size: 724 179 5092 Fragments arranged by size: 5092 724 179 EcoRI G'AATT_C Cuts at: 3304 3682 3304 Size: 378 5617 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2248 2248 Size: 5995 FspI TGC'GCA Cuts at: 4501 4501 Size: 5995 HaeII r_GCGC'y Cuts at: 1678 5142 1678 Size: 3464 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3172 3204 4636 4668 4710 Size: 32 135 32 1600 32 1432 32 42 Cuts at: 4710 4742 1373 Size: 32 2626 Fragments arranged by size: 2626 1600 1432 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3339 3585 669 Size: 988 281 1401 246 3079 Fragments arranged by size: 3079 1401 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5995 HpaI GTT'AAC Cuts at: 3585 3585 Size: 5995 KpnI G_GTAC'C Cuts at: 3320 3320 Size: 5995 MfeI C'AATT_G Cuts at: 3594 3594 Size: 5995 MluI A'CGCG_T Cuts at: 5871 5871 Size: 5995 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3073 3315 5109 5293 1305 Size: 1768 242 1794 184 2007 Fragments arranged by size: 2007 1794 1768 242 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4173 Fragments arranged by size: 4173 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2034 2537 2639 3050 3953 4312 4471 Size: 648 868 503 102 411 903 359 159 Cuts at: 4471 518 Size: 2042 Fragments arranged by size: 2042 903 868 648 503 411 359 159 102 MspA1I CmG'CkG Cuts at: 1606 2317 2390 2399 2933 3411 4088 5236 5481 Size: 711 73 9 534 478 677 1148 245 Cuts at: 5481 1606 Size: 2120 Fragments arranged by size: 2120 1148 711 677 534 478 245 73 9 NcoI C'CATG_G Cuts at: 513 1065 2004 2959 513 Size: 552 939 955 3549 Fragments arranged by size: 3549 955 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5995 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5995 NotI GC'GGCC_GC Cuts at: 3442 3442 Size: 5995 NsiI A_TGCA'T Cuts at: 2031 2031 Size: 5995 NspI r_CATG'y Cuts at: 1132 1229 2204 2338 3025 3353 4898 1132 Size: 97 975 134 687 328 1545 2229 Fragments arranged by size: 2229 1545 975 687 328 134 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5995 PciI A'CATG_T Cuts at: 3021 4894 3021 Size: 1873 4122 PflMI CCAn_nnn'nTGG Cuts at: 1108 2176 1108 Size: 1068 4927 PmeI GTTT'AAAC Cuts at: 3299 3299 Size: 5995 PmlI CAC'GTG Cuts at: 2539 2661 2539 Size: 122 5873 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4064 4096 5577 5609 4064 Size: 32 1481 32 4450 Fragments arranged by size: 4450 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5995 PsiI TTA'TAA Cuts at: 3565 3565 Size: 5995 PspOMI G'GGCC_C Cuts at: 3084 3084 Size: 5995 PstI C_TGCA'G Cuts at: 830 3347 830 Size: 2517 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5995 PvuII CAG'CTG Cuts at: 2317 2933 2317 Size: 616 5379 SalI G'TCGA_C Cuts at: 1936 3337 1936 Size: 1401 4594 SbfI CC_TGCA'GG Cuts at: 3347 3347 Size: 5995 ScaI AGT'ACT Cuts at: 4243 5955 4243 Size: 1712 4283 SfcI C'TryA_G Cuts at: 826 1045 3255 3343 4478 5159 5350 826 Size: 219 2210 88 1135 681 191 1471 Fragments arranged by size: 2210 1471 1135 681 219 191 88 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5995 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5995 SmaI CCC'GGG Cuts at: 1872 3322 1872 Size: 1450 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2648 4099 5000 5262 820 Size: 197 926 705 1451 901 262 1553 Fragments arranged by size: 1553 1451 926 901 705 262 197 SnaBI TAC'GTA Cuts at: 493 3786 493 Size: 3293 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5995 SphI G_CATG'C Cuts at: 1132 3353 1132 Size: 2221 3774 SspI AAT'ATT Cuts at: 5 52 2756 2914 3919 5 Size: 47 2704 158 1005 2081 Fragments arranged by size: 2704 2081 1005 158 47 StuI AGG'CCT Cuts at: 1517 2516 1517 Size: 999 4996 StyI C'CwwG_G Cuts at: 513 1065 2004 2959 3079 3434 513 Size: 552 939 955 120 355 3074 Fragments arranged by size: 3074 955 939 552 355 120 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2117 2549 4022 4039 4192 4377 4822 Size: 1255 537 432 1473 17 153 185 445 Cuts at: 4822 325 Size: 1498 Fragments arranged by size: 1498 1473 1255 537 445 432 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3183 4241 5953 Size: 274 80 33 51 1443 1206 1058 1712 Cuts at: 5953 96 Size: 138 Fragments arranged by size: 1712 1443 1206 1058 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3087 3119 5955 5987 3087 Size: 32 2836 32 3095 Fragments arranged by size: 3095 2836 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5995 XbaI T'CTAG_A Cuts at: 3331 3331 Size: 5995 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5995 XmaI C'CCGG_G Cuts at: 1870 3320 1870 Size: 1450 4545 XmnI GAAnn'nnTTC Cuts at: 4124 4124 Size: 5995 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3803 276 Size: 53 83 186 936 2269 2468 Fragments arranged by size: 2468 2269 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI