Restriction map for
pFN21AB4009
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# NO CUT ENZYMES ( 84 enzymes) #
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Acc65I AclI AhdI Alw44I AlwI
ApaLI AscI Asp718I AspEI AspI
AvrII BalI BamHI BbvCI BcgI
BglII BlnI BsaBI BsiWI BsmBI
BsmI Bsp1407I BspDI BspHI BspLU11I
BsrDI BsrGI BssHII BssSI Bst1107I
BstYI BstZ17I ClaI CpoI DraI
DraIII DrdI Eam1105I EcoRV EcoT22I
FseI HindIII HpaI KpnI MamI
MfeI MflI MluI MluNI MscI
MunI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
PacI PaeR7I PflFI PmeI PshAI
Psp1406I PvuI RcaI RsrII SalI
ScaI SfiI SgrAI SnaBI SpeI
SphI SspBI SspI StuI SwaI
Tth111I XbaI XhoI XhoII
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# ONE CUT ENZYMES ( 62 enzymes) #
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AatII 1327 GACGTC GACGT^C
AccI 2062 GTCTAC GT^[AC][GT]AC
AccIII 1155 TCCGGA T^CCGGA
AflII 255 CTTAAG C^TTAAG
AflIII 1202 ACACGT A^C[GA][CT]GT
AgeI 2304 ACCGGT A^CCGGT
AlwNI 1480 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1420 AGCGCT AGC^GCT
ApaI 2187 GGGCCC GGGCC^C
Asp700I 856 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 1871 TGCGCA TGC^GCA
BbeI 1594 GGCGCC GGCGC^C
BbrPI 1006 CACGTG CAC^GTG
BclI 381 TGATCA T^GATCA
BfrI 255 CTTAAG C^TTAAG
BglI 1567 GCCCTGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1887 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1887 GCTGAGC GC^T[ACGT]AGC
BsaAI 1006 CACGTG [CT]AC^GT[GA]
BseAI 1155 TCCGGA T^CCGGA
BsiEI 1736 CGGCCG CG[GA][CT]^CG
BspEI 1155 TCCGGA T^CCGGA
BstAPI 36 GCAAGTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2366 TTCGAA TT^CGAA
BstEII 1755 GGTGACC G^GT[ACGT]ACC
BstPI 1755 GGTGACC G^GT[ACGT]ACC
BstXI 1760 CCAAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1887 GCTGAGC GC^T[ACGT]AGC
DpnII 382 GATC ^GATC
EagI 1736 CGGCCG C^GGCCG
EclXI 1736 CGGCCG C^GGCCG
Eco47III 1420 AGCGCT AGC^GCT
Eco52I 1736 CGGCCG C^GGCCG
EcoNI 677 CCTTGGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1755 GGTGACC G^GT[ACGT]ACC
EcoRI 2287 GAATTC G^AATTC
FbaI 381 TGATCA T^GATCA
FspI 1871 TGCGCA TGC^GCA
HincII 1456 GTCAAC GT[CT]^[GA]AC
HindII 1456 GTCAAC GT[CT]^[GA]AC
KasI 1594 GGCGCC G^GCGCC
KspI 1605 CCGCGG CCGC^GG
MboI 382 GATC ^GATC
MroI 1155 TCCGGA T^CCGGA
NarI 1594 GGCGCC GG^CGCC
NdeII 382 GATC ^GATC
NlaIII 847 CATG CATG^
NspV 2366 TTCGAA TT^CGAA
PflMI 1200 CCACACGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 2304 ACCGGT A^CCGGT
PmaCI 1006 CACGTG CAC^GTG
PmlI 1006 CACGTG CAC^GTG
SacII 1605 CCGCGG CCGC^GG
Sau3AI 382 GATC ^GATC
SbfI 1569 CCTGCAGG CCTGCA^GG
SexAI 1751 ACCTGGT A^CC[AT]GGT
SfoI 1594 GGCGCC GGC^GCC
SfuI 2366 TTCGAA TT^CGAA
Sse8387I 1569 CCTGCAGG CCTGCA^GG
Van91I 1200 CCACACGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1907 CCAAGCACAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 856 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
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AcsI 721 GAATTT [GA]^AATT[CT]
2287 GAATTC
ApoI 721 GAATTT [GA]^AATT[CT]
2287 GAATTC
AseI 703 ATTAAT AT^TAAT
880 ATTAAT
AsnI 703 ATTAAT AT^TAAT
880 ATTAAT
BbsI 1159 GAAGAC GAAGAC(2/6)
2091 GTCTTC
BciVI 1280 GGATAC GTATCC(6/5)
1106 GTATCC
BpmI 90 CTGGAG CTGGAG(16/14)
893 CTGGAG
BpuAI 1159 GAAGAC GAAGAC(2/6)
2091 GTCTTC
BsaWI 1155 TCCGGA [AT]^CCGG[AT]
2304 ACCGGT
BseRI 277 GAGGAG GAGGAG(10/8)
2145 GAGGAG
BsrFI 2031 ACCGGC [GA]^CCGG[CT]
2304 ACCGGT
Cfr10I 2031 ACCGGC [GA]^CCGG[CT]
2304 ACCGGT
HgaI 1657 GACGC GACGC(5/10)
1900 GACGC
MaeIII 1267 GTCAC ^GT[ACGT]AC
1756 GTGAC
MslI 537 CATTAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1308 CACAACTGTG
PshBI 703 ATTAAT AT^TAAT
880 ATTAAT
PstI 1570 CTGCAG CTGCA^G
1693 CTGCAG
PvuII 226 CAGCTG CAG^CTG
1813 CAGCTG
SapI 607 GAAGAGC GCTCTTC(1/4)
2097 GCTCTTC
SfaNI 764 GCATC GCATC(5/9)
2202 GCATC
SmaI 1730 CCCGGG CCC^GGG
1834 CCCGGG
SmlI 255 CTTAAG C^T[CT][GA]AG
1819 CTCAAG
TfiI 488 GAATC G^A[AT]TC
750 GATTC
Tsp45I 1267 GTCAC ^GT[GC]AC
1756 GTGAC
XmaCI 1730 CCCGGG C^CCGGG
1834 CCCGGG
XmaI 1730 CCCGGG C^CCGGG
1834 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AocI AspHI AvaI AvaII BanI
BanII BbvI BcnI BfaI BmyI
BsaHI BsaI BsaJI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrI BssKI
BstF5I BstNI BstUI Bsu36I Cac8I
CfoI Cfr13I DdeI DraII DsaI
EaeI EarI Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MboII MnlI MseI
MspA1I MspI MvaI MvnI MwoI
NciI NlaIV PleI PpuMI RsaI
SacI Sau96I ScrFI SfcI StyI
TaqI Tru9I TseI Tsp509I TspRI
TthHB8I