(Circular) (Six-base) MAPSORT of: pFN21AB4009.seq Check: 9022 from: 1 to: 7096 pFN21AB4009 7096 bp With 182 enzymes: SgfI * February 27, 2009 14:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2314 3504 2314 Size: 1190 5906 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3336 4906 278 Size: 53 83 186 936 1800 1570 2468 Fragments arranged by size: 2468 1800 1570 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4068 4439 1937 Size: 2131 371 4594 Fragments arranged by size: 4594 2131 371 Acc65I G'GTAC_C Cuts at: 4417 4417 Size: 7096 AclI AA'CG_TT Cuts at: 1563 5223 5596 1563 Size: 3660 373 3063 Fragments arranged by size: 3660 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2186 2351 3174 5159 6543 6811 1632 Size: 554 165 823 1985 1384 268 1917 Fragments arranged by size: 1985 1917 1384 823 554 268 165 AfeI AGC'GCT Cuts at: 3427 3427 Size: 7096 AflII C'TTAA_G Cuts at: 820 1017 2260 820 Size: 197 1243 5656 Fragments arranged by size: 5656 1243 197 AflIII A'CryG_T Cuts at: 1203 3207 5995 6972 1203 Size: 2004 2788 977 1327 Fragments arranged by size: 2788 2004 1327 977 AgeI A'CCGG_T Cuts at: 4309 5927 4309 Size: 1618 5478 AhdI GACnn_n'nnGTC Cuts at: 5825 5825 Size: 7096 AleI CACnn'nnGTG Cuts at: 1166 3317 1166 Size: 2151 4945 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7096 AlwNI CAG_nnn'CTG Cuts at: 3490 4839 6411 3490 Size: 1349 1572 4175 Fragments arranged by size: 4175 1572 1349 ApaI G_GGCC'C Cuts at: 4196 4196 Size: 7096 ApaLI G'TGCA_C Cuts at: 5153 6309 5153 Size: 1156 5940 ApoI r'AATT_y Cuts at: 1493 2726 4292 4405 4631 4783 6892 1493 Size: 1233 1566 113 226 152 2109 1697 Fragments arranged by size: 2109 1697 1566 1233 226 152 113 AseI AT'TA_AT Cuts at: 160 1032 2709 2886 5650 160 Size: 872 1677 177 2764 1606 Fragments arranged by size: 2764 1677 1606 872 177 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7096 AvaI C'yCGr_G Cuts at: 1870 1943 2196 2579 3735 3790 3839 4421 1870 Size: 73 253 383 1156 55 49 582 4545 Fragments arranged by size: 4545 1156 582 383 253 73 55 49 BamHI G'GATC_C Cuts at: 4426 4793 4426 Size: 367 6729 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3446 3599 3730 4033 4183 Size: 325 217 627 1659 153 131 303 150 Cuts at: 4183 4226 4417 5872 618 Size: 43 191 1455 1842 Fragments arranged by size: 1842 1659 1455 627 325 303 217 191 153 150 131 43 BanII G_rGCy'C Cuts at: 1391 2087 2197 2289 2619 2628 3840 4196 4415 Size: 696 110 92 330 9 1212 356 219 Cuts at: 4415 1391 Size: 4072 Fragments arranged by size: 4072 1212 696 356 330 219 110 92 9 BbeI G_GCGC'C Cuts at: 3603 3603 Size: 7096 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3171 4089 928 Size: 971 1272 918 3935 Fragments arranged by size: 3935 1272 971 918 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5287 5321 1903 Size: 34 3350 34 3678 Fragments arranged by size: 3678 3350 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3122 3279 4988 6198 3122 Size: 157 1709 1210 4020 Fragments arranged by size: 4020 1709 1210 157 BclI T'GATC_A Cuts at: 1548 2386 1548 Size: 838 6258 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3578 5707 136 Size: 107 122 71 631 605 1906 2129 1525 Fragments arranged by size: 2129 1906 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7091 7091 Size: 7096 BlpI GC'TnA_GC Cuts at: 3893 4513 3893 Size: 620 6476 Bme1580I G_kGCm'C Cuts at: 1790 3735 4196 5157 6313 1790 Size: 1945 461 961 1156 2573 Fragments arranged by size: 2573 1945 1156 961 461 BmgBI CAC'GTC Cuts at: 1318 4219 1318 Size: 2901 4195 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2256 4827 5785 Size: 416 53 83 399 24 839 2571 958 Cuts at: 5785 442 Size: 1753 Fragments arranged by size: 2571 1753 958 839 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7096 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2965 2965 Size: 7096 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2116 2919 5756 1239 Size: 877 803 2837 2579 Fragments arranged by size: 2837 2579 877 803 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3809 5221 6086 6384 3809 Size: 1412 865 298 4521 Fragments arranged by size: 4521 1412 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2209 3083 3217 3331 3520 5759 882 Size: 617 710 874 134 114 189 2239 2219 Fragments arranged by size: 2239 2219 874 710 617 189 134 114 BsaAI yAC'GTr Cuts at: 493 1204 3013 4887 493 Size: 711 1809 1874 2702 Fragments arranged by size: 2702 1874 1809 711 BsaBI GATnn'nnATC Cuts at: 7090 7090 Size: 7096 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3333 3600 3663 4903 Size: 53 83 186 936 1800 267 63 1240 Cuts at: 4903 5285 5920 275 Size: 382 635 1451 Fragments arranged by size: 1800 1451 1240 936 635 382 267 186 83 63 53 BsaWI w'CCGG_w Cuts at: 3160 4309 5529 5927 6201 6348 6759 3160 Size: 1149 1220 398 274 147 411 3497 Fragments arranged by size: 3497 1220 1149 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2403 2433 3599 3629 3986 Size: 30 165 30 1000 30 1166 30 357 Cuts at: 3986 4016 1178 Size: 30 4258 Fragments arranged by size: 4258 1166 1000 357 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2297 4165 1186 Size: 1111 1868 4117 Fragments arranged by size: 4117 1868 1111 BseYI C'CCAG_C Cuts at: 4005 6299 6795 4005 Size: 2294 496 4306 Fragments arranged by size: 4306 2294 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3392 3476 3703 4165 1163 Size: 573 1656 84 227 462 4094 Fragments arranged by size: 4094 1656 573 462 227 84 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3744 4546 5307 6335 1540 Size: 275 186 1743 802 761 1028 2301 Fragments arranged by size: 2301 1743 1028 802 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2289 2619 2628 2920 3245 3309 3987 4122 5157 Size: 330 9 292 325 64 678 135 1035 Cuts at: 5157 5242 6313 2289 Size: 85 1071 3072 Fragments arranged by size: 3072 1071 1035 678 330 325 292 135 85 64 9 BsmI GAATG_Cn' Cuts at: 4607 4700 4607 Size: 93 7003 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2087 2197 2289 2619 2628 2920 3245 Size: 399 297 110 92 330 9 292 325 Cuts at: 3245 3309 3735 3840 3987 4122 4196 4415 5157 Size: 64 426 105 147 135 74 219 742 Cuts at: 5157 5242 6313 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 426 399 330 325 297 292 219 147 135 110 105 92 85 74 64 9 BspEI T'CCGG_A Cuts at: 3160 6759 3160 Size: 3599 3497 BspHI T'CATG_A Cuts at: 4985 6715 4985 Size: 1730 5366 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2314 3498 3504 4023 4451 844 Size: 1470 1184 6 519 428 3489 Fragments arranged by size: 3489 1470 1184 519 428 6 BsrBI CCG'CTC Cuts at: 1394 1823 2127 4021 4224 4983 1394 Size: 429 304 1894 203 759 3507 Fragments arranged by size: 3507 1894 759 429 304 203 BsrDI GCAATG_nn' Cuts at: 65 1248 5591 5765 65 Size: 1183 4343 174 1396 Fragments arranged by size: 4343 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4036 4309 5740 5927 1700 Size: 110 2226 273 1431 187 2869 Fragments arranged by size: 2869 2226 1431 273 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7096 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7096 BssSI C'ACGA_G Cuts at: 5156 6168 5156 Size: 1012 6084 BstAPI GCAn_nnn'nTGC Cuts at: 794 2047 794 Size: 1253 5843 BstBI TT'CG_AA Cuts at: 4372 5900 4372 Size: 1528 5568 BstEII G'GTnAC_C Cuts at: 1184 3760 1184 Size: 2576 4520 BstXI CCAn_nnnn'nTGG Cuts at: 3772 3772 Size: 7096 BstYI r'GATC_y Cuts at: 1972 4426 4793 5178 5195 6647 6733 6745 7091 Size: 2454 367 385 17 1452 86 12 346 Cuts at: 7091 1972 Size: 1977 Fragments arranged by size: 2454 1977 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3551 4026 4188 3551 Size: 475 162 6459 Fragments arranged by size: 6459 475 162 BtgI C'CryG_G Cuts at: 513 1065 2004 3093 3610 3831 513 Size: 552 939 1089 517 221 3778 Fragments arranged by size: 3778 1089 939 552 517 221 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6357 Fragments arranged by size: 6357 683 56 BtsI GCAGTG_nn' Cuts at: 3424 4612 5406 5426 3424 Size: 1188 794 20 5094 Fragments arranged by size: 5094 1188 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7096 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7061 DraI TTT'AAA Cuts at: 4400 4400 Size: 7096 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6103 809 Size: 507 4787 1802 Fragments arranged by size: 4787 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3535 3741 3909 Size: 54 1425 50 275 18 1705 206 168 Cuts at: 3909 3963 4543 5432 7061 8 Size: 54 580 889 1629 43 Fragments arranged by size: 1705 1629 1425 889 580 275 206 168 54 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3741 4543 1537 Size: 275 1929 802 4090 Fragments arranged by size: 4090 1929 802 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2607 4109 4115 5026 1353 Size: 1254 1502 6 911 3423 Fragments arranged by size: 3423 1502 1254 911 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5683 6057 6203 387 Size: 1064 138 4094 374 146 1280 Fragments arranged by size: 4094 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2287 2617 2626 2287 Size: 330 9 6757 Fragments arranged by size: 6757 330 9 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2116 2186 2351 2919 3174 5159 5756 Size: 393 484 70 165 568 255 1985 597 Cuts at: 5756 6543 6811 1239 Size: 787 268 1524 Fragments arranged by size: 1985 1524 787 597 568 484 393 268 255 165 70 EcoNI CCTnn'n_nnAGG Cuts at: 2686 2686 Size: 7096 EcoO109I rG'GnC_Cy Cuts at: 2155 2658 3487 3571 4192 4193 4233 2155 Size: 503 829 84 621 1 40 5018 Fragments arranged by size: 5018 829 621 503 84 40 1 EcoRI G'AATT_C Cuts at: 4292 4405 4783 4292 Size: 113 378 6605 Fragments arranged by size: 6605 378 113 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2066 4213 2066 Size: 2147 4949 FspI TGC'GCA Cuts at: 3878 5602 3878 Size: 1724 5372 FspAI rTGC'GCAy Cuts at: 3878 3878 Size: 7096 HaeII r_GCGC'y Cuts at: 1678 2603 3429 3603 3641 6243 1678 Size: 925 826 174 38 2602 2531 Fragments arranged by size: 2602 2531 925 826 174 38 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2011 2043 2642 2674 2798 Size: 32 135 32 439 32 599 32 124 Cuts at: 2798 2830 3110 3142 3425 3457 3599 3631 3773 Size: 32 280 32 283 32 142 32 142 Cuts at: 3773 3805 3986 4018 5737 5769 5811 5843 1373 Size: 32 181 32 1719 32 42 32 2626 Fragments arranged by size: 2626 1719 599 439 283 280 181 142 142 135 124 42 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3463 4440 4686 669 Size: 988 281 1525 977 246 3079 Fragments arranged by size: 3079 1525 988 977 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7096 HpaI GTT'AAC Cuts at: 4686 4686 Size: 7096 KasI G'GCGC_C Cuts at: 3599 3599 Size: 7096 KpnI G_GTAC'C Cuts at: 4421 4421 Size: 7096 MfeI C'AATT_G Cuts at: 4695 4695 Size: 7096 MluI A'CGCG_T Cuts at: 6972 6972 Size: 7096 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3192 6210 6394 1305 Size: 1887 3018 184 2007 Fragments arranged by size: 3018 2007 1887 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5274 Fragments arranged by size: 5274 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2546 3317 5054 5413 5572 518 Size: 648 1380 771 1737 359 159 2042 Fragments arranged by size: 2042 1737 1380 771 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2233 3612 3697 3820 4512 5189 6337 6582 Size: 627 1379 85 123 692 677 1148 245 Cuts at: 6582 1606 Size: 2120 Fragments arranged by size: 2120 1379 1148 692 677 627 245 123 85 NarI GG'CG_CC Cuts at: 3600 3600 Size: 7096 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5605 Fragments arranged by size: 5605 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7096 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7096 NotI GC'GGCC_GC Cuts at: 4543 4543 Size: 7096 NspI r_CATG'y Cuts at: 1132 1229 4454 5999 1132 Size: 97 3225 1545 2229 Fragments arranged by size: 3225 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7096 PciI A'CATG_T Cuts at: 5995 5995 Size: 7096 PflMI CCAn_nnn'nTGG Cuts at: 1108 3211 1108 Size: 2103 4993 PfoI T'CCnGG_A Cuts at: 2619 2619 Size: 7096 PmeI GTTT'AAAC Cuts at: 4400 4400 Size: 7096 PmlI CAC'GTG Cuts at: 3013 3013 Size: 7096 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5165 5197 6678 6710 5165 Size: 32 1481 32 5551 Fragments arranged by size: 5551 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2155 2658 3487 4233 2155 Size: 503 829 746 5018 Fragments arranged by size: 5018 829 746 503 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7096 PsiI TTA'TAA Cuts at: 4666 4666 Size: 7096 PspOMI G'GGCC_C Cuts at: 4192 4192 Size: 7096 PstI C_TGCA'G Cuts at: 830 3579 3702 4448 830 Size: 2749 123 746 3478 Fragments arranged by size: 3478 2749 746 123 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7096 PvuII CAG'CTG Cuts at: 2233 3820 2233 Size: 1587 5509 SacI G_AGCT'C Cuts at: 2289 2619 2628 2289 Size: 330 9 6757 Fragments arranged by size: 6757 330 9 SacII CC_GC'GG Cuts at: 3613 3613 Size: 7096 SalI G'TCGA_C Cuts at: 1936 4438 1936 Size: 2502 4594 SanDI GG'GwC_CC Cuts at: 2658 4233 2658 Size: 1575 5521 SapI GCTCTTCn'nnn_ Cuts at: 2607 4109 2607 Size: 1502 5594 SbfI CC_TGCA'GG Cuts at: 3579 4448 3579 Size: 869 6227 ScaI AGT'ACT Cuts at: 5344 7056 5344 Size: 1712 5384 SexAI A'CCwGG_T Cuts at: 3756 3756 Size: 7096 SfcI C'TryA_G Cuts at: 826 1045 2997 3429 3575 3698 4444 5579 6260 Size: 219 1952 432 146 123 746 1135 681 Cuts at: 6260 6451 826 Size: 191 1471 Fragments arranged by size: 1952 1471 1135 746 681 432 219 191 146 123 SfoI GGC'GCC Cuts at: 3601 3601 Size: 7096 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7096 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7096 SmaI CCC'GGG Cuts at: 1872 3737 3841 4423 1872 Size: 1865 104 582 4545 Fragments arranged by size: 4545 1865 582 104 SmlI C'TyrA_G Cuts at: 820 1017 1943 2260 3824 5200 6101 6363 820 Size: 197 926 317 1564 1376 901 262 1553 Fragments arranged by size: 1564 1553 1376 926 901 317 262 197 SnaBI TAC'GTA Cuts at: 493 4887 493 Size: 4394 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7096 SphI G_CATG'C Cuts at: 1132 4454 1132 Size: 3322 3774 SrfI GCCC'GGGC Cuts at: 3841 3841 Size: 7096 SspI AAT'ATT Cuts at: 5 52 5020 5 Size: 47 4968 2081 Fragments arranged by size: 4968 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7096 StyI C'CwwG_G Cuts at: 513 1065 2004 2014 2682 2773 3765 4535 513 Size: 552 939 10 668 91 992 770 3074 Fragments arranged by size: 3074 992 939 770 668 552 91 10 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3374 5123 5140 5293 5478 5923 325 Size: 1255 1794 1749 17 153 185 445 1498 Fragments arranged by size: 1794 1749 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3252 5342 7054 Size: 274 80 33 51 1443 1275 2090 1712 Cuts at: 7054 96 Size: 138 Fragments arranged by size: 2090 1712 1443 1275 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4336 4368 7056 7088 4336 Size: 32 2688 32 4344 Fragments arranged by size: 4344 2688 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7096 XbaI T'CTAG_A Cuts at: 4432 4432 Size: 7096 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3919 3919 Size: 7096 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7096 XmaI C'CCGG_G Cuts at: 1870 3735 3839 4421 1870 Size: 1865 104 582 4545 Fragments arranged by size: 4545 1865 582 104 XmnI GAAnn'nnTTC Cuts at: 2865 5225 2865 Size: 2360 4736 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3334 4904 276 Size: 53 83 186 936 1800 1570 2468 Fragments arranged by size: 2468 1800 1570 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoRV FseI NaeI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SfiI SwaI