Restriction map for
pFN21AB4009
(Linear) (Six-base) MAPSORT of: KIBB4009.seq Check: 2457 from: 1 to: 2391
KIBB4009.orf
With 182 enzymes: SgfI *
July 18, 2007 13:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 309 1499 2391
Size: 309 1190 892
Fragments arranged by size:
1190 892 309
AatII G_ACGT'C
Cuts at: 0 1331 2391
Size: 1331 1060
AccI GT'mk_AC
Cuts at: 0 2063 2391
Size: 2063 328
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 346 1169 2391
Size: 181 165 823 1222
Fragments arranged by size:
1222 823 181 165
AfeI AGC'GCT
Cuts at: 0 1422 2391
Size: 1422 969
AflII C'TTAA_G
Cuts at: 0 255 2391
Size: 255 2136
AflIII A'CryG_T
Cuts at: 0 1202 2391
Size: 1202 1189
AgeI A'CCGG_T
Cuts at: 0 2304 2391
Size: 2304 87
AleI CACnn'nnGTG
Cuts at: 0 1312 2391
Size: 1312 1079
AlwNI CAG_nnn'CTG
Cuts at: 0 1485 2391
Size: 1485 906
ApaI G_GGCC'C
Cuts at: 0 2191 2391
Size: 2191 200
ApoI r'AATT_y
Cuts at: 0 721 2287 2391
Size: 721 1566 104
Fragments arranged by size:
1566 721 104
AseI AT'TA_AT
Cuts at: 0 704 881 2391
Size: 704 177 1510
Fragments arranged by size:
1510 704 177
AvaI C'yCGr_G
Cuts at: 0 191 574 1730 1785 1834 2391
Size: 191 383 1156 55 49 557
Fragments arranged by size:
1156 557 383 191 55 49
BanI G'GyrC_C
Cuts at: 0 1441 1594 1725 2028 2178 2221 2391
Size: 1441 153 131 303 150 43 170
Fragments arranged by size:
1441 303 170 153 150 131 43
BanII G_rGCy'C
Cuts at: 0 82 192 284 614 623 1835 2191 2391
Size: 82 110 92 330 9 1212 356 200
Fragments arranged by size:
1212 356 330 200 110 92 82 9
BbeI G_GCGC'C
Cuts at: 0 1598 2391
Size: 1598 793
BbsI GAAGACnn'nnnn_
Cuts at: 0 1166 2084 2391
Size: 1166 918 307
Fragments arranged by size:
1166 918 307
BciVI GTATCCnnnnn_n'
Cuts at: 0 1117 1274 2391
Size: 1117 157 1117
Fragments arranged by size:
1117 1117 157
BclI T'GATC_A
Cuts at: 0 381 2391
Size: 381 2010
BglI GCCn_nnn'nGGC
Cuts at: 0 1573 2391
Size: 1573 818
BlpI GC'TnA_GC
Cuts at: 0 1888 2391
Size: 1888 503
Bme1580I G_kGCm'C
Cuts at: 0 1730 2191 2391
Size: 1730 461 200
Fragments arranged by size:
1730 461 200
BmgBI CAC'GTC
Cuts at: 0 2214 2391
Size: 2214 177
BmrI ACTGGGnnnn_n'
Cuts at: 0 251 2391
Size: 251 2140
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 960 2391
Size: 960 1431
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 111 914 2391
Size: 111 803 1477
Fragments arranged by size:
1477 803 111
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1804 2391
Size: 1804 587
BsaI GGTCTCn'nnnn_
Cuts at: 0 204 1078 1212 1326 1515 2391
Size: 204 874 134 114 189 876
Fragments arranged by size:
876 874 204 189 134 114
BsaAI yAC'GTr
Cuts at: 0 1008 2391
Size: 1008 1383
BsaHI Gr'CG_yC
Cuts at: 0 1328 1595 1658 2391
Size: 1328 267 63 733
Fragments arranged by size:
1328 733 267 63
BsaWI w'CCGG_w
Cuts at: 0 1155 2304 2391
Size: 1155 1149 87
Fragments arranged by size:
1155 1149 87
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 398 428 1594 1624 1981 2011 2391
Size: 398 30 1166 30 357 30 380
Fragments arranged by size:
1166 398 380 357 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 292 2160 2391
Size: 292 1868 231
Fragments arranged by size:
1868 292 231
BseYI C'CCAG_C
Cuts at: 0 2000 2391
Size: 2000 391
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1387 1471 1698 2160 2391
Size: 1387 84 227 462 231
Fragments arranged by size:
1387 462 231 227 84
BsiEI CG_ry'CG
Cuts at: 0 1739 2391
Size: 1739 652
BsiHKAI G_wGCw'C
Cuts at: 0 284 614 623 915 1240 1304 1982 2117
Size: 284 330 9 292 325 64 678 135
Cuts at: 2117 2391
Size: 274
Fragments arranged by size:
678 330 325 292 284 274 135 64
9
Bsp1286I G_dGCh'C
Cuts at: 0 82 192 284 614 623 915 1240 1304
Size: 82 110 92 330 9 292 325 64
Cuts at: 1304 1730 1835 1982 2117 2191 2391
Size: 426 105 147 135 74 200
Fragments arranged by size:
426 330 325 292 200 147 135 110
105 92 82 74 64 9
BspEI T'CCGG_A
Cuts at: 0 1155 2391
Size: 1155 1236
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 309 1493 1499 2018 2391
Size: 309 1184 6 519 373
Fragments arranged by size:
1184 519 373 309 6
BsrBI CCG'CTC
Cuts at: 0 122 2016 2219 2391
Size: 122 1894 203 172
Fragments arranged by size:
1894 203 172 122
BsrFI r'CCGG_y
Cuts at: 0 2031 2304 2391
Size: 2031 273 87
Fragments arranged by size:
2031 273 87
BstAPI GCAn_nnn'nTGC
Cuts at: 0 42 2391
Size: 42 2349
BstBI TT'CG_AA
Cuts at: 0 2367 2391
Size: 2367 24
BstEII G'GTnAC_C
Cuts at: 0 1755 2391
Size: 1755 636
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1767 2391
Size: 1767 624
Bsu36I CC'TnA_GG
Cuts at: 0 1546 2021 2183 2391
Size: 1546 475 162 208
Fragments arranged by size:
1546 475 208 162
BtgI C'CryG_G
Cuts at: 0 1088 1605 1826 2391
Size: 1088 517 221 565
Fragments arranged by size:
1088 565 517 221
BtsI GCAGTG_nn'
Cuts at: 0 1419 2391
Size: 1419 972
EaeI y'GGCC_r
Cuts at: 0 1530 1736 1904 1958 2391
Size: 1530 206 168 54 433
Fragments arranged by size:
1530 433 206 168 54
EagI C'GGCC_G
Cuts at: 0 1736 2391
Size: 1736 655
EarI CTCTTCn'nnn_
Cuts at: 0 602 2104 2110 2391
Size: 602 1502 6 281
Fragments arranged by size:
1502 602 281 6
EcoICRI GAG'CTC
Cuts at: 0 282 612 621 2391
Size: 282 330 9 1770
Fragments arranged by size:
1770 330 282 9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 111 181 346 914 1169 2391
Size: 111 70 165 568 255 1222
Fragments arranged by size:
1222 568 255 165 111 70
EcoNI CCTnn'n_nnAGG
Cuts at: 0 681 2391
Size: 681 1710
EcoO109I rG'GnC_Cy
Cuts at: 0 150 653 1482 1566 2187 2188 2228 2391
Size: 150 503 829 84 621 1 40 163
Fragments arranged by size:
829 621 503 163 150 84 40 1
EcoRI G'AATT_C
Cuts at: 0 2287 2391
Size: 2287 104
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 61 2208 2391
Size: 61 2147 183
Fragments arranged by size:
2147 183 61
FspI TGC'GCA
Cuts at: 0 1873 2391
Size: 1873 518
FspAI rTGC'GCAy
Cuts at: 0 1873 2391
Size: 1873 518
HaeII r_GCGC'y
Cuts at: 0 598 1424 1598 1636 2391
Size: 598 826 174 38 755
Fragments arranged by size:
826 755 598 174 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 6 38 637 669 793 825 1105 1137
Size: 6 32 599 32 124 32 280 32
Cuts at: 1137 1420 1452 1594 1626 1768 1800 1981 2013
Size: 283 32 142 32 142 32 181 32
Cuts at: 2013 2391
Size: 378
Fragments arranged by size:
599 378 283 280 181 142 142 124
32 32 32 32 32 32 32 32
6
HincII GTy'rAC
Cuts at: 0 1458 2391
Size: 1458 933
KasI G'GCGC_C
Cuts at: 0 1594 2391
Size: 1594 797
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1187 2391
Size: 1187 1204
MslI CAynn'nnrTG
Cuts at: 0 541 1312 2391
Size: 541 771 1079
Fragments arranged by size:
1079 771 541
MspA1I CmG'CkG
Cuts at: 0 228 1607 1692 1815 2391
Size: 228 1379 85 123 576
Fragments arranged by size:
1379 576 228 123 85
NarI GG'CG_CC
Cuts at: 0 1595 2391
Size: 1595 796
PflMI CCAn_nnn'nTGG
Cuts at: 0 1206 2391
Size: 1206 1185
PfoI T'CCnGG_A
Cuts at: 0 614 2391
Size: 614 1777
PmlI CAC'GTG
Cuts at: 0 1008 2391
Size: 1008 1383
PpuMI rG'GwC_Cy
Cuts at: 0 150 653 1482 2228 2391
Size: 150 503 829 746 163
Fragments arranged by size:
829 746 503 163 150
PspOMI G'GGCC_C
Cuts at: 0 2187 2391
Size: 2187 204
PstI C_TGCA'G
Cuts at: 0 1574 1697 2391
Size: 1574 123 694
Fragments arranged by size:
1574 694 123
PvuII CAG'CTG
Cuts at: 0 228 1815 2391
Size: 228 1587 576
Fragments arranged by size:
1587 576 228
SacI G_AGCT'C
Cuts at: 0 284 614 623 2391
Size: 284 330 9 1768
Fragments arranged by size:
1768 330 284 9
SacII CC_GC'GG
Cuts at: 0 1608 2391
Size: 1608 783
SanDI GG'GwC_CC
Cuts at: 0 653 2228 2391
Size: 653 1575 163
Fragments arranged by size:
1575 653 163
SapI GCTCTTCn'nnn_
Cuts at: 0 602 2104 2391
Size: 602 1502 287
Fragments arranged by size:
1502 602 287
SbfI CC_TGCA'GG
Cuts at: 0 1574 2391
Size: 1574 817
SexAI A'CCwGG_T
Cuts at: 0 1751 2391
Size: 1751 640
SfcI C'TryA_G
Cuts at: 0 992 1424 1570 1693 2391
Size: 992 432 146 123 698
Fragments arranged by size:
992 698 432 146 123
SfoI GGC'GCC
Cuts at: 0 1596 2391
Size: 1596 795
SmaI CCC'GGG
Cuts at: 0 1732 1836 2391
Size: 1732 104 555
Fragments arranged by size:
1732 555 104
SmlI C'TyrA_G
Cuts at: 0 255 1819 2391
Size: 255 1564 572
Fragments arranged by size:
1564 572 255
SrfI GCCC'GGGC
Cuts at: 0 1836 2391
Size: 1836 555
StyI C'CwwG_G
Cuts at: 0 9 677 768 1760 2391
Size: 9 668 91 992 631
Fragments arranged by size:
992 668 631 91 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1369 2391
Size: 1369 1022
TatI w'GTAC_w
Cuts at: 0 1247 2391
Size: 1247 1144
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2331 2363 2391
Size: 2331 32 28
Fragments arranged by size:
2331 32 28
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1914 2391
Size: 1914 477
XmaI C'CCGG_G
Cuts at: 0 1730 1834 2391
Size: 1730 104 557
Fragments arranged by size:
1730 557 104
XmnI GAAnn'nnTTC
Cuts at: 0 860 2391
Size: 860 1531
ZraI GAC'GTC
Cuts at: 0 1329 2391
Size: 1329 1062
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflII AflIII AgeI
AleI AlwNI ApaI ApoI AseI AvaI BanI BanII
BbeI BbsI BciVI BclI BglI BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspMI BsrBI BsrFI BstAPI BstBI BstEII BstXI Bsu36I
BtgI BtsI EaeI EagI EarI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII
KasI MmeI MslI MspA1I NarI PflMI PfoI PmlI
PpuMI PspOMI PstI PvuII SacI SacII SanDI SapI
SbfI SexAI SfcI SfoI SmaI SmlI SrfI StyI
TaqII TatI TstI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AhdI AlfI AloI ApaLI AscI AsiSI
AvrII BaeI BamHI BbvCI BcgI BfrBI BglII BmtI
Bpu10I BsaBI BsiWI BsmI BsmBI BspHI BsrDI BsrGI
BssHII BssSI BstYI BstZ17I BtgZI ClaI CspCI DraI
DraIII DrdI EciI EcoRV FseI HindIII HpaI KpnI
MfeI MluI MscI NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PmeI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SalI
ScaI SfiI SgfI SgrAI SnaBI SpeI SphI SspI
StuI SwaI Tth111I XbaI XhoI