Restriction map for
pFN21AB3903
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# NO CUT ENZYMES ( 75 enzymes) #
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AatII Acc65I AclI AflII AgeI
AhdI AscI Asp718I AspEI AviII
AvrII BbrPI BbsI BciVI BfrI
BlnI BlpI Bpu1102I BpuAI BsaBI
BsiWI BsmBI BsmI BspDI BspLU11I
BsrFI BssSI Bst1107I BstAPI BstZ17I
CelII Cfr10I ClaI CpoI DraI
DraIII Eam1105I EcoRI EcoT22I FseI
FspI HindIII HpaI KpnI MamI
MfeI MluI MunI NaeI NdeI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PvuI
RsrII SapI ScaI SexAI SfiI
SgrAI SnaBI SphI SwaI XhoI
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# ONE CUT ENZYMES ( 75 enzymes) #
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AccI 1449 GTCGAC GT^[AC][GT]AC
AccIII 2220 TCCGGA T^CCGGA
AcsI 817 AAATTT [GA]^AATT[CT]
AcyI 2282 GGCGCC G[GA]^CG[CT]C
AflIII 431 ACGTGT A^C[GA][CT]GT
Alw44I 1534 GTGCAC G^TGCAC
AlwNI 1382 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1506 CCTCAGG CC^T[ACGT]AGG
Aor51HI 525 AGCGCT AGC^GCT
ApaLI 1534 GTGCAC G^TGCAC
ApoI 817 AAATTT [GA]^AATT[CT]
AspI 838 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 2 TGGCCA TGG^CCA
BanI 2282 GGCGCC G^G[CT][GA]CC
BbeI 2282 GGCGCC GGCGC^C
BcgI 1438 GCAATTACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1736 TGATCA T^GATCA
BglI 211 GCCAAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 430 TACGTG [CT]AC^GT[GA]
BsaHI 2282 GGCGCC G[GA]^CG[CT]C
BsaI 2058 GGTCTC GGTCTC(1/5)
BseAI 2220 TCCGGA T^CCGGA
BsgI 735 CTGCAC GTGCAG(16/14)
Bsp1407I 1487 TGTACA T^GTACA
BspEI 2220 TCCGGA T^CCGGA
BspHI 2069 TCATGA T^CATGA
BsrDI 1686 CATTGC GCAATG(2/0)
BsrGI 1487 TGTACA T^GTACA
BssHII 220 GCGCGC G^CGCGC
BstBI 605 TTCGAA TT^CGAA
BstEII 393 GGTCACC G^GT[ACGT]ACC
BstPI 393 GGTCACC G^GT[ACGT]ACC
BstXI 1664 CCACTTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1506 CCTCAGG CC^T[ACGT]AGG
DrdI 712 GACTCAGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 58 CGGCCG C^GGCCG
EclXI 58 CGGCCG C^GGCCG
Eco47III 525 AGCGCT AGC^GCT
Eco52I 58 CGGCCG C^GGCCG
Eco81I 1506 CCTCAGG CC^T[ACGT]AGG
EcoNI 345 CCTGCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 393 GGTCACC G^GT[ACGT]ACC
EcoRV 688 GATATC GAT^ATC
FbaI 1736 TGATCA T^GATCA
HgaI 938 GACGC GACGC(5/10)
Hin1I 2282 GGCGCC G[GA]^CG[CT]C
HincII 1449 GTCGAC GT[CT]^[GA]AC
HindII 1449 GTCGAC GT[CT]^[GA]AC
KasI 2282 GGCGCC G^GCGCC
MluNI 2 TGGCCA TGG^CCA
MroI 2220 TCCGGA T^CCGGA
MscI 2 TGGCCA TGG^CCA
MslI 1665 CACTTATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 2282 GGCGCC GG^CGCC
NspV 605 TTCGAA TT^CGAA
PflFI 838 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1809 CCAGATCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 2069 TCATGA T^CATGA
SalI 1449 GTCGAC G^TCGAC
SbfI 183 CCTGCAGG CCTGCA^GG
SfoI 2282 GGCGCC GGC^GCC
SfuI 605 TTCGAA TT^CGAA
SmaI 2256 CCCGGG CCC^GGG
SmlI 1171 CTTGAG C^T[CT][GA]AG
SpeI 1027 ACTAGT A^CTAGT
Sse8387I 183 CCTGCAGG CCTGCA^GG
SspBI 1487 TGTACA T^GTACA
SspI 478 AATATT AAT^ATT
StuI 2134 AGGCCT AGG^CCT
Tth111I 838 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1809 CCAGATCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2082 TCTAGA T^CTAGA
XcmI 1809 CCAGATCTTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 2256 CCCGGG C^CCGGG
XmaI 2256 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AseI 481 ATTAAT AT^TAAT
1878 ATTAAT
AsnI 481 ATTAAT AT^TAAT
1878 ATTAAT
Asp700I 448 GAACCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
814 GAAAAATTTC
BamHI 638 GGATCC G^GATCC
913 GGATCC
BsiEI 58 CGGCCG CG[GA][CT]^CG
279 CGACCG
BspMI 344 ACCTGC ACCTGC(4/8)
753 GCAGGT
BsrBI 321 CCGCTC CCGCTC(-3/-3)
2266 GAGCGG
EaeI 2 TGGCCA [CT]^GGCC[GA]
58 CGGCCG
EarI 1166 CTCTTC CTCTTC(1/4)
1801 CTCTTC
HaeII 525 AGCGCT [GA]GCGC^[CT]
2282 GGCGCC
Ksp632I 1166 CTCTTC CTCTTC(1/4)
1801 CTCTTC
KspI 55 CCGCGG CCGC^GG
1922 CCGCGG
MaeII 338 ACGT A^CGT
431 ACGT
NcoI 1481 CCATGG C^CATGG
1518 CCATGG
NspI 802 GCATGT [GA]CATG^[CT]
1979 ACATGC
PshBI 481 ATTAAT AT^TAAT
1878 ATTAAT
PvuII 896 CAGCTG CAG^CTG
1727 CAGCTG
SacI 313 GAGCTC GAGCT^C
1390 GAGCTC
SacII 55 CCGCGG CCGC^GG
1922 CCGCGG
XmnI 448 GAACCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
814 GAAAAATTTC
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# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
ApaI AspHI AvaI AvaII BanII
BbvCI BbvI BcnI BfaI BglII
BmyI BpmI BsaJI BsaWI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeI
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII