(Circular) (Six-base) MAPSORT of: pFN21AB3903.seq Check: 1886 from: 1 to: 7003 pFN21AB3903 7003 bp With 182 enzymes: SgfI * February 27, 2009 14:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4813 278 Size: 53 83 186 936 3277 2468 Fragments arranged by size: 3277 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3455 4346 1937 Size: 1518 891 4594 Fragments arranged by size: 4594 1518 891 Acc65I G'GTAC_C Cuts at: 4324 4324 Size: 7003 AclI AA'CG_TT Cuts at: 1563 5130 5503 1563 Size: 3567 373 3063 Fragments arranged by size: 3567 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2897 2946 3153 3734 3884 5066 6450 6718 Size: 1265 49 207 581 150 1182 1384 268 Cuts at: 6718 1632 Size: 1917 Fragments arranged by size: 1917 1384 1265 1182 581 268 207 150 49 AfeI AGC'GCT Cuts at: 2532 2532 Size: 7003 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6806 AflIII A'CryG_T Cuts at: 1203 2436 5902 6879 1203 Size: 1233 3466 977 1327 Fragments arranged by size: 3466 1327 1233 977 AgeI A'CCGG_T Cuts at: 5834 5834 Size: 7003 AhdI GACnn_n'nnGTC Cuts at: 5732 5732 Size: 7003 AleI CACnn'nnGTG Cuts at: 1166 3674 1166 Size: 2508 4495 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3084 1184 Size: 1900 5103 AlwNI CAG_nnn'CTG Cuts at: 3392 4746 6318 3392 Size: 1354 1572 4077 Fragments arranged by size: 4077 1572 1354 ApaI G_GGCC'C Cuts at: 2086 2120 2937 2086 Size: 34 817 6152 Fragments arranged by size: 6152 817 34 ApaLI G'TGCA_C Cuts at: 3539 5060 6216 3539 Size: 1521 1156 4326 Fragments arranged by size: 4326 1521 1156 ApoI r'AATT_y Cuts at: 1493 2822 4312 4538 4690 6799 1493 Size: 1329 1490 226 152 2109 1697 Fragments arranged by size: 2109 1697 1490 1329 226 152 AseI AT'TA_AT Cuts at: 160 1032 2487 3884 5557 160 Size: 872 1455 1397 1673 1606 Fragments arranged by size: 1673 1606 1455 1397 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7003 AvaI C'yCGr_G Cuts at: 1870 1943 2113 2526 4261 4328 1870 Size: 73 170 413 1735 67 4545 Fragments arranged by size: 4545 1735 413 170 73 67 BamHI G'GATC_C Cuts at: 2643 2918 4333 4700 2643 Size: 275 1415 367 4946 Fragments arranged by size: 4946 1415 367 275 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4287 4324 5779 618 Size: 325 217 627 2500 37 1455 1842 Fragments arranged by size: 2500 1842 1455 627 325 217 37 BanII G_rGCy'C Cuts at: 1391 2086 2120 2136 2322 2937 3399 4155 4322 Size: 695 34 16 186 615 462 756 167 Cuts at: 4322 1391 Size: 4072 Fragments arranged by size: 4072 756 695 615 462 186 167 34 16 BbeI G_GCGC'C Cuts at: 4291 4291 Size: 7003 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6032 BbvCI CC'TCA_GC Cuts at: 2198 2899 3530 3993 2198 Size: 701 631 463 5208 Fragments arranged by size: 5208 701 631 463 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3432 3466 5194 5228 1903 Size: 34 1495 34 1728 34 3678 Fragments arranged by size: 3678 1728 1495 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4895 6105 4895 Size: 1210 5793 BclI T'GATC_A Cuts at: 1548 3741 1548 Size: 2193 4810 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2222 5614 136 Size: 107 122 71 631 605 550 3392 1525 Fragments arranged by size: 3392 1525 631 605 550 122 107 71 BglII A'GATC_T Cuts at: 3080 3430 3802 3816 6998 3080 Size: 350 372 14 3182 3085 Fragments arranged by size: 3182 3085 372 350 14 BlpI GC'TnA_GC Cuts at: 4420 4420 Size: 7003 Bme1580I G_kGCm'C Cuts at: 1790 2086 2120 2161 2937 3543 5064 6220 1790 Size: 296 34 41 776 606 1521 1156 2573 Fragments arranged by size: 2573 1521 1156 776 606 296 41 34 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7003 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2136 3617 3862 Size: 416 53 83 399 24 719 1481 245 Cuts at: 3862 4734 5692 442 Size: 872 958 1753 Fragments arranged by size: 1753 1481 958 872 719 416 399 245 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7003 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4174 4174 Size: 7003 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2051 2263 3001 3666 3779 3854 4146 5663 Size: 812 212 738 665 113 75 292 1517 Cuts at: 5663 1239 Size: 2579 Fragments arranged by size: 2579 1517 812 738 665 292 212 113 75 Bpu10I CC'TnA_GC Cuts at: 2198 2899 3108 3530 3993 2198 Size: 701 209 422 463 5208 Fragments arranged by size: 5208 701 463 422 209 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3197 5128 5993 6291 3197 Size: 1931 865 298 3909 Fragments arranged by size: 3909 1931 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4069 5666 882 Size: 617 2570 1597 2219 Fragments arranged by size: 2570 2219 1597 617 BsaAI yAC'GTr Cuts at: 493 1204 2437 4794 493 Size: 711 1233 2357 2702 Fragments arranged by size: 2702 2357 1233 711 BsaBI GATnn'nnATC Cuts at: 6997 6997 Size: 7003 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4288 4810 5192 5827 Size: 53 83 186 936 2755 522 382 635 Cuts at: 5827 275 Size: 1451 Fragments arranged by size: 2755 1451 936 635 522 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2235 2292 4225 5436 5834 6108 6255 6666 2235 Size: 57 1933 1211 398 274 147 411 2572 Fragments arranged by size: 2572 1933 1211 411 398 274 147 57 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6778 Fragments arranged by size: 6778 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2083 2300 2536 3389 4196 1186 Size: 897 217 236 853 807 3993 Fragments arranged by size: 3993 897 853 807 236 217 BseYI C'CCAG_C Cuts at: 2087 2978 3824 6206 6702 2087 Size: 891 846 2382 496 2388 Fragments arranged by size: 2388 2382 891 846 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2725 1163 Size: 573 989 5441 Fragments arranged by size: 5441 989 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2066 2287 4453 5214 6242 1540 Size: 275 186 65 221 2166 761 1028 2301 Fragments arranged by size: 2301 2166 1028 761 275 221 186 65 BsiHKAI G_wGCw'C Cuts at: 2037 2322 3399 3543 5064 5149 6220 2037 Size: 285 1077 144 1521 85 1071 2820 Fragments arranged by size: 2820 1521 1077 1071 285 144 85 BsmI GAATG_Cn' Cuts at: 4514 4607 4514 Size: 93 6910 BsmBI CGTCTCn'nnnn_ Cuts at: 4293 4293 Size: 7003 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2037 2086 2120 2136 2161 2322 2937 Size: 399 247 49 34 16 25 161 615 Cuts at: 2937 3399 3543 4155 4322 5064 5149 6220 1391 Size: 462 144 612 167 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 615 612 462 399 247 167 161 144 85 49 34 25 16 BspEI T'CCGG_A Cuts at: 4225 6666 4225 Size: 2441 4562 BspHI T'CATG_A Cuts at: 4074 4892 6622 4074 Size: 818 1730 4455 Fragments arranged by size: 4455 1730 818 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2358 2749 4358 844 Size: 1514 391 1609 3489 Fragments arranged by size: 3489 1609 1514 391 BsrBI CCG'CTC Cuts at: 1394 1823 2328 4273 4890 1394 Size: 429 505 1945 617 3507 Fragments arranged by size: 3507 1945 617 505 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3690 5498 5672 65 Size: 1183 2442 1808 174 1396 Fragments arranged by size: 2442 1808 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5647 5834 1700 Size: 110 3837 187 2869 Fragments arranged by size: 3837 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3492 96 Size: 3396 3607 BssHII G'CGCG_C Cuts at: 1925 2225 1925 Size: 300 6703 BssSI C'ACGA_G Cuts at: 5063 6075 5063 Size: 1012 5991 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7003 BstBI TT'CG_AA Cuts at: 2611 5807 2611 Size: 3196 3807 BstEII G'GTnAC_C Cuts at: 1184 2398 1184 Size: 1214 5789 BstXI CCAn_nnnn'nTGG Cuts at: 3676 3676 Size: 7003 BstYI r'GATC_y Cuts at: 1972 2643 2918 3080 3430 3802 3816 4185 4333 Size: 671 275 162 350 372 14 369 148 Cuts at: 4333 4700 5085 5102 6554 6640 6652 6998 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 671 385 372 369 367 350 346 275 162 148 86 17 14 12 Bsu36I CC'TnA_GG Cuts at: 3512 3512 Size: 7003 BtgI C'CryG_G Cuts at: 513 1065 2004 2060 3486 3523 3927 513 Size: 552 939 56 1426 37 404 3589 Fragments arranged by size: 3589 1426 939 552 404 56 37 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6264 Fragments arranged by size: 6264 683 56 BtsI GCAGTG_nn' Cuts at: 2161 3487 4519 5313 5333 2161 Size: 1326 1032 794 20 3831 Fragments arranged by size: 3831 1326 1032 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7003 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3277 3312 529 Size: 35 2713 35 4220 Fragments arranged by size: 4220 2713 35 35 DraI TTT'AAA Cuts at: 4307 4307 Size: 7003 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2723 6010 809 Size: 507 1407 3287 1802 Fragments arranged by size: 3287 1802 1407 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2063 4450 Size: 54 1425 50 275 18 177 56 2387 Cuts at: 4450 5339 6968 8 Size: 889 1629 43 Fragments arranged by size: 2387 1629 1425 889 275 177 56 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2063 4450 1537 Size: 275 251 2387 4090 Fragments arranged by size: 4090 2387 275 251 EarI CTCTTCn'nnn_ Cuts at: 1353 3177 3812 4933 1353 Size: 1824 635 1121 3423 Fragments arranged by size: 3423 1824 1121 635 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5590 5964 6110 387 Size: 1064 138 4001 374 146 1280 Fragments arranged by size: 4001 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2320 3397 2320 Size: 1077 5926 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2051 2263 2897 2946 3001 3153 3666 Size: 393 419 212 634 49 55 152 513 Cuts at: 3666 3734 3779 3854 3884 4146 5066 5663 6450 Size: 68 45 75 30 262 920 597 787 Cuts at: 6450 6718 1239 Size: 268 1524 Fragments arranged by size: 1524 920 787 634 597 513 419 393 268 262 212 152 75 68 55 49 45 30 EcoNI CCTnn'n_nnAGG Cuts at: 2354 2354 Size: 7003 EcoO109I rG'GnC_Cy Cuts at: 2082 2083 2117 2194 2260 2934 3641 2082 Size: 1 34 77 66 674 707 5444 Fragments arranged by size: 5444 707 674 77 66 34 1 EcoRI G'AATT_C Cuts at: 4312 4690 4312 Size: 378 6625 EcoRV GAT'ATC Cuts at: 2695 2695 Size: 7003 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2245 2245 Size: 7003 FspI TGC'GCA Cuts at: 5509 5509 Size: 7003 HaeII r_GCGC'y Cuts at: 1678 2534 4291 6150 1678 Size: 856 1757 1859 2531 Fragments arranged by size: 2531 1859 1757 856 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3055 3087 3953 3985 5644 Size: 32 135 32 1483 32 866 32 1659 Cuts at: 5644 5676 5718 5750 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1659 1483 866 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3456 4347 4593 669 Size: 988 281 1518 891 246 3079 Fragments arranged by size: 3079 1518 988 891 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7003 HpaI GTT'AAC Cuts at: 4593 4593 Size: 7003 KasI G'GCGC_C Cuts at: 4287 4287 Size: 7003 KpnI G_GTAC'C Cuts at: 4328 4328 Size: 7003 MfeI C'AATT_G Cuts at: 4602 4602 Size: 7003 MluI A'CGCG_T Cuts at: 6879 6879 Size: 7003 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2316 3053 6117 6301 1305 Size: 1011 737 3064 184 2007 Fragments arranged by size: 3064 2007 1011 737 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 10 Size: 54 1425 343 177 5004 Fragments arranged by size: 5004 1425 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 3674 4961 5320 5479 518 Size: 648 2508 1287 359 159 2042 Fragments arranged by size: 2508 2042 1287 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2062 2903 3734 3929 4134 4256 4419 5096 Size: 456 841 831 195 205 122 163 677 Cuts at: 5096 6244 6489 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 841 831 677 456 245 205 195 163 122 NarI GG'CG_CC Cuts at: 4288 4288 Size: 7003 NcoI C'CATG_G Cuts at: 513 1065 2004 3486 3523 513 Size: 552 939 1482 37 3993 Fragments arranged by size: 3993 1482 939 552 37 NdeI CA'TA_TG Cuts at: 387 387 Size: 7003 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7003 NotI GC'GGCC_GC Cuts at: 4450 4450 Size: 7003 NspI r_CATG'y Cuts at: 1132 1229 2811 3988 4361 5906 1132 Size: 97 1582 1177 373 1545 2229 Fragments arranged by size: 2229 1582 1545 1177 373 97 PasI CC'CwG_GG Cuts at: 1348 2356 2930 3951 1348 Size: 1008 574 1021 4400 Fragments arranged by size: 4400 1021 1008 574 PciI A'CATG_T Cuts at: 5902 5902 Size: 7003 PflMI CCAn_nnn'nTGG Cuts at: 1108 3820 1108 Size: 2712 4291 PfoI T'CCnGG_A Cuts at: 2850 3037 3795 2850 Size: 187 758 6058 Fragments arranged by size: 6058 758 187 PmeI GTTT'AAAC Cuts at: 4307 4307 Size: 7003 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5072 5104 6585 6617 5072 Size: 32 1481 32 5458 Fragments arranged by size: 5458 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7003 PsiI TTA'TAA Cuts at: 4573 4573 Size: 7003 PspOMI G'GGCC_C Cuts at: 2082 2116 2933 2082 Size: 34 817 6152 Fragments arranged by size: 6152 817 34 PstI C_TGCA'G Cuts at: 830 2168 2193 4115 4355 830 Size: 1338 25 1922 240 3478 Fragments arranged by size: 3478 1922 1338 240 25 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7003 PvuII CAG'CTG Cuts at: 2903 3734 2903 Size: 831 6172 SacI G_AGCT'C Cuts at: 2322 3399 2322 Size: 1077 5926 SacII CC_GC'GG Cuts at: 2063 3930 2063 Size: 1867 5136 SalI G'TCGA_C Cuts at: 1936 3454 4345 1936 Size: 1518 891 4594 Fragments arranged by size: 4594 1518 891 SbfI CC_TGCA'GG Cuts at: 2193 4355 2193 Size: 2162 4841 ScaI AGT'ACT Cuts at: 5251 6963 5251 Size: 1712 5291 SfcI C'TryA_G Cuts at: 826 1045 2164 2189 4111 4351 5486 6167 6358 Size: 219 1119 25 1922 240 1135 681 191 Cuts at: 6358 826 Size: 1471 Fragments arranged by size: 1922 1471 1135 1119 681 240 219 191 25 SfoI GGC'GCC Cuts at: 4289 4289 Size: 7003 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7003 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7003 SmaI CCC'GGG Cuts at: 1872 4263 4330 1872 Size: 2391 67 4545 Fragments arranged by size: 4545 2391 67 SmlI C'TyrA_G Cuts at: 820 1017 1943 3176 5107 6008 6270 820 Size: 197 926 1233 1931 901 262 1553 Fragments arranged by size: 1931 1553 1233 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4794 493 Size: 4301 2702 SpeI A'CTAG_T Cuts at: 152 3032 152 Size: 2880 4123 SphI G_CATG'C Cuts at: 1132 4361 1132 Size: 3229 3774 SspI AAT'ATT Cuts at: 5 52 2485 4927 5 Size: 47 2433 2442 2081 Fragments arranged by size: 2442 2433 2081 47 StuI AGG'CCT Cuts at: 1517 4141 1517 Size: 2624 4379 StyI C'CwwG_G Cuts at: 513 1065 2004 2121 3486 3523 3876 4442 513 Size: 552 939 117 1365 37 353 566 3074 Fragments arranged by size: 3074 1365 939 566 552 353 117 37 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3682 3943 5030 5047 5200 5385 5830 Size: 1255 2102 261 1087 17 153 185 445 Cuts at: 5830 325 Size: 1498 Fragments arranged by size: 2102 1498 1255 1087 445 261 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3462 3492 5249 Size: 274 80 33 51 1443 1485 30 1757 Cuts at: 5249 6961 96 Size: 1712 138 Fragments arranged by size: 1757 1712 1485 1443 274 138 80 51 33 30 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6963 6995 6963 Size: 32 6971 Tth111I GACn'n_nGTC Cuts at: 1316 2846 1316 Size: 1530 5473 XbaI T'CTAG_A Cuts at: 4087 4339 4087 Size: 252 6751 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3821 3821 Size: 7003 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7003 XmaI C'CCGG_G Cuts at: 1870 4261 4328 1870 Size: 2391 67 4545 Fragments arranged by size: 4545 2391 67 XmnI GAAnn'nnTTC Cuts at: 2457 2823 5132 2457 Size: 366 2309 4328 Fragments arranged by size: 4328 2309 366 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4811 276 Size: 53 83 186 936 3277 2468 Fragments arranged by size: 3277 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I DraIII FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SanDI SapI SexAI SfiI SrfI SwaI