Restriction map for
pFN21AB3844
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# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
AlwI AocI Aor51HI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvrII BamHI BbvCI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BlpI Bpu1102I BsaBI BsaWI
BseAI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstYI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI Eam1105I
Eco47III Eco81I EcoRI EcoT22I FbaI
FseI HindIII HpaI KpnI MaeI
MamI MfeI MflI MluI MroI
MseI MunI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SalI ScaI SexAI SfiI SfuI
SgrAI SpeI SspBI SspI StuI
SwaI TfiI Tru9I Tth111I XbaI
XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 27 enzymes) #
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Alw44I 1729 GTGCAC G^TGCAC
ApaI 1074 GGGCCC GGGCC^C
ApaLI 1729 GTGCAC G^TGCAC
BbsI 1690 GTCTTC GAAGAC(2/6)
BpuAI 1690 GTCTTC GAAGAC(2/6)
BsmBI 1138 GAGACG CGTCTC(1/5)
DpnII 276 GATC ^GATC
EagI 1040 CGGCCG C^GGCCG
EclXI 1040 CGGCCG C^GGCCG
Eco52I 1040 CGGCCG C^GGCCG
EcoRV 232 GATATC GAT^ATC
MboI 276 GATC ^GATC
NdeII 276 GATC ^GATC
NspI 645 GCATGC [GA]CATG^[CT]
PflMI 1715 CCATCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 299 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 964 CAGCTG CAG^CTG
SacI 754 GAGCTC GAGCT^C
SapI 543 GCTCTTC GCTCTTC(1/4)
Sau3AI 276 GATC ^GATC
SmaI 1754 CCCGGG CCC^GGG
SnaBI 1546 TACGTA TAC^GTA
SphI 645 GCATGC GCATG^C
Tsp509I 772 AATT ^AATT
Van91I 1715 CCATCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 1754 CCCGGG C^CCGGG
XmaI 1754 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 38 enzymes) #
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AspHI 754 GAGCTC G[AT]GC[AT]^C
1729 GTGCAC
AvaI 579 CTCGGG C^[CT]CG[GA]G
1754 CCCGGG
AviII 416 TGCGCA TGC^GCA
767 TGCGCA
BbeI 674 GGCGCC GGCGC^C
1303 GGCGCC
BbrPI 1324 CACGTG CAC^GTG
1359 CACGTG
BglI 1162 GCCAATGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1333 GCCCTGACGGC
BsaI 1444 GAGACC GGTCTC(1/5)
1531 GAGACC
BsiEI 204 CGACCG CG[GA][CT]^CG
1040 CGGCCG
BsiHKAI 754 GAGCTC G[AT]GC[AT]^C
1729 GTGCAC
BsoBI 579 CTCGGG C^[CT]CG[GA]G
1754 CCCGGG
BsrBI 378 CCGCTC CCGCTC(-3/-3)
889 GAGCGG
BsrDI 423 CATTGC GCAATG(2/0)
1538 GCAATG
BsrI 98 ACTGG ACTGG(1/-1)
1022 ACTGG
BstEII 691 GGTAACC G^GT[ACGT]ACC
1521 GGTCACC
BstPI 691 GGTAACC G^GT[ACGT]ACC
1521 GGTCACC
BstXI 1115 CCAGCGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1307 CCAAGACCCTGG
EarI 544 CTCTTC CTCTTC(1/4)
1696 CTCTTC
EcoNI 132 CCTGACGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1935 CCTCTGCAAGG
EcoO65I 691 GGTAACC G^GT[ACGT]ACC
1521 GGTCACC
FspI 416 TGCGCA TGC^GCA
767 TGCGCA
HgaI 244 GACGC GACGC(5/10)
511 GACGC
HincII 316 GTCAAC GT[CT]^[GA]AC
1048 GTCAAC
HindII 316 GTCAAC GT[CT]^[GA]AC
1048 GTCAAC
KasI 674 GGCGCC G^GCGCC
1303 GGCGCC
Ksp632I 544 CTCTTC CTCTTC(1/4)
1696 CTCTTC
KspI 930 CCGCGG CCGC^GG
1842 CCGCGG
NarI 674 GGCGCC GG^CGCC
1303 GGCGCC
PmaCI 1324 CACGTG CAC^GTG
1359 CACGTG
PmlI 1324 CACGTG CAC^GTG
1359 CACGTG
PshAI 135 GACGGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
309 GACCAAGGTC
SacII 930 CCGCGG CCGC^GG
1842 CCGCGG
SbfI 399 CCTGCAGG CCTGCA^GG
828 CCTGCAGG
SfoI 674 GGCGCC GGC^GCC
1303 GGCGCC
Sse8387I 399 CCTGCAGG CCTGCA^GG
828 CCTGCAGG
TaqI 146 TCGA T^CGA
941 TCGA
Tsp45I 52 GTCAC ^GT[GC]AC
1522 GTCAC
TthHB8I 146 TCGA T^CGA
941 TCGA
XcmI 74 CCACGGCCACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
428 CCAGGGACTCGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI AvaII BalI BanI BanII
BbvI BcnI BmyI BpmI BsaAI
BsaHI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BssKI BstAPI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeII MaeIII
MboII MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NlaIII NlaIV PleI
PstI RsaI Sau96I ScrFI SfaNI
SfcI SmlI StyI TseI TspRI