Restriction map for
pFN21AB3844
(Linear) (Six-base) MAPSORT of: KIBB3844.seq Check: 8603 from: 1 to: 2022
KIBB3844.orf
With 182 enzymes: SgfI *
July 18, 2007 13:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 166 407 821 2022
Size: 166 241 414 1201
Fragments arranged by size:
1201 414 241 166
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 714 2022
Size: 714 1308
AleI CACnn'nnGTG
Cuts at: 0 64 1566 2022
Size: 64 1502 456
Fragments arranged by size:
1502 456 64
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 469 854 2022
Size: 469 385 1168
Fragments arranged by size:
1168 469 385
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 88 120 2022
Size: 88 32 1902
Fragments arranged by size:
1902 88 32
AlwNI CAG_nnn'CTG
Cuts at: 0 969 1863 1937 2022
Size: 969 894 74 85
Fragments arranged by size:
969 894 85 74
ApaI G_GGCC'C
Cuts at: 0 1078 2022
Size: 1078 944
ApaLI G'TGCA_C
Cuts at: 0 1729 2022
Size: 1729 293
AvaI C'yCGr_G
Cuts at: 0 579 1754 2022
Size: 579 1175 268
Fragments arranged by size:
1175 579 268
BanI G'GyrC_C
Cuts at: 0 674 1303 1966 2022
Size: 674 629 663 56
Fragments arranged by size:
674 663 629 56
BanII G_rGCy'C
Cuts at: 0 122 758 1078 1435 1496 2022
Size: 122 636 320 357 61 526
Fragments arranged by size:
636 526 357 320 122 61
BbeI G_GCGC'C
Cuts at: 0 678 1307 2022
Size: 678 629 715
Fragments arranged by size:
715 678 629
BbsI GAAGACnn'nnnn_
Cuts at: 0 1683 2022
Size: 1683 339
BglI GCCn_nnn'nGGC
Cuts at: 0 1168 1339 2022
Size: 1168 171 683
Fragments arranged by size:
1168 683 171
Bme1580I G_kGCm'C
Cuts at: 0 1078 1733 2022
Size: 1078 655 289
Fragments arranged by size:
1078 655 289
BmrI ACTGGGnnnn_n'
Cuts at: 0 1032 2022
Size: 1032 990
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 478 619 907 1510 2022
Size: 478 141 288 603 512
Fragments arranged by size:
603 512 478 288 141
Bpu10I CC'TnA_GC
Cuts at: 0 550 565 973 2022
Size: 550 15 408 1049
Fragments arranged by size:
1049 550 408 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 520 1219 2022
Size: 289 231 699 803
Fragments arranged by size:
803 699 289 231
BsaI GGTCTCn'nnnn_
Cuts at: 0 1438 1525 2022
Size: 1438 87 497
Fragments arranged by size:
1438 497 87
BsaAI yAC'GTr
Cuts at: 0 1326 1361 1548 1728 1899 2022
Size: 1326 35 187 180 171 123
Fragments arranged by size:
1326 187 180 171 123 35
BsaHI Gr'CG_yC
Cuts at: 0 245 512 675 1304 2022
Size: 245 267 163 629 718
Fragments arranged by size:
718 629 267 245 163
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 613 643 1483 1513 2022
Size: 613 30 840 30 509
Fragments arranged by size:
840 613 509 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 520 616 1374 1906 2022
Size: 520 96 758 532 116
Fragments arranged by size:
758 532 520 116 96
BseYI C'CCAG_C
Cuts at: 0 802 962 1422 2022
Size: 802 160 460 600
Fragments arranged by size:
802 600 460 160
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 343 379 451 1474 2022
Size: 343 36 72 1023 548
Fragments arranged by size:
1023 548 343 72 36
BsiEI CG_ry'CG
Cuts at: 0 207 1043 2022
Size: 207 836 979
Fragments arranged by size:
979 836 207
BsiHKAI G_wGCw'C
Cuts at: 0 758 1733 2022
Size: 758 975 289
Fragments arranged by size:
975 758 289
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1132 2022
Size: 1132 890
Bsp1286I G_dGCh'C
Cuts at: 0 122 758 1078 1435 1496 1733 2022
Size: 122 636 320 357 61 237 289
Fragments arranged by size:
636 357 320 289 237 122 61
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 98 166 269 315 380 407 704 806
Size: 98 68 103 46 65 27 297 102
Cuts at: 806 821 836 1484 2022
Size: 15 15 648 538
Fragments arranged by size:
648 538 297 103 102 98 68 65
46 27 15 15
BsrBI CCG'CTC
Cuts at: 0 380 891 2022
Size: 380 511 1131
Fragments arranged by size:
1131 511 380
BsrDI GCAATG_nn'
Cuts at: 0 422 1545 2022
Size: 422 1123 477
Fragments arranged by size:
1123 477 422
BstAPI GCAn_nnn'nTGC
Cuts at: 0 158 1863 1937 2022
Size: 158 1705 74 85
Fragments arranged by size:
1705 158 85 74
BstEII G'GTnAC_C
Cuts at: 0 691 1521 2022
Size: 691 830 501
Fragments arranged by size:
830 691 501
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1122 1314 2022
Size: 1122 192 708
Fragments arranged by size:
1122 708 192
BtgI C'CryG_G
Cuts at: 0 74 83 930 1043 1181 1286 1292 1427
Size: 74 9 847 113 138 105 6 135
Cuts at: 1427 1526 1640 1842 2022
Size: 99 114 202 180
Fragments arranged by size:
847 202 180 138 135 114 113 105
99 74 9 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 108 699 2022
Size: 108 591 1323
Fragments arranged by size:
1323 591 108
BtsI GCAGTG_nn'
Cuts at: 0 23 41 1685 2022
Size: 23 18 1644 337
Fragments arranged by size:
1644 337 23 18
EaeI y'GGCC_r
Cuts at: 0 56 77 701 779 869 1040 1097 1160
Size: 56 21 624 78 90 171 57 63
Cuts at: 1160 1184 1289 1316 2022
Size: 24 105 27 706
Fragments arranged by size:
706 624 171 105 90 78 63 57
56 27 24 21
EagI C'GGCC_G
Cuts at: 0 1040 2022
Size: 1040 982
EarI CTCTTCn'nnn_
Cuts at: 0 550 1702 2022
Size: 550 1152 320
Fragments arranged by size:
1152 550 320
EcoICRI GAG'CTC
Cuts at: 0 756 2022
Size: 756 1266
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 478 619 714 907 1510 2022
Size: 478 141 95 193 603 512
Fragments arranged by size:
603 512 478 193 141 95
EcoNI CCTnn'n_nnAGG
Cuts at: 0 136 1939 2022
Size: 136 1803 83
Fragments arranged by size:
1803 136 83
EcoO109I rG'GnC_Cy
Cuts at: 0 300 657 1059 1074 1075 1332 1763 2022
Size: 300 357 402 15 1 257 431 259
Fragments arranged by size:
431 402 357 300 259 257 15 1
EcoRV GAT'ATC
Cuts at: 0 234 2022
Size: 234 1788
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 561 2022
Size: 561 1461
FspI TGC'GCA
Cuts at: 0 418 769 2022
Size: 418 351 1253
Fragments arranged by size:
1253 418 351
FspAI rTGC'GCAy
Cuts at: 0 418 2022
Size: 418 1604
HaeII r_GCGC'y
Cuts at: 0 678 809 1307 2022
Size: 678 131 498 715
Fragments arranged by size:
715 678 498 131
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 466 498 1483 1515 2022
Size: 466 32 985 32 507
Fragments arranged by size:
985 507 466 32 32
HincII GTy'rAC
Cuts at: 0 318 1050 2022
Size: 318 732 972
Fragments arranged by size:
972 732 318
KasI G'GCGC_C
Cuts at: 0 674 1303 2022
Size: 674 629 719
Fragments arranged by size:
719 674 629
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 929 1295 1946 2022
Size: 929 366 651 76
Fragments arranged by size:
929 651 366 76
MscI TGG'CCA
Cuts at: 0 703 871 1099 1162 1291 1318 2022
Size: 703 168 228 63 129 27 704
Fragments arranged by size:
704 703 228 168 129 63 27
MslI CAynn'nnrTG
Cuts at: 0 64 473 1324 1516 1566 2022
Size: 64 409 851 192 50 456
Fragments arranged by size:
851 456 409 192 64 50
MspA1I CmG'CkG
Cuts at: 0 176 453 642 932 966 1844 2022
Size: 176 277 189 290 34 878 178
Fragments arranged by size:
878 290 277 189 178 176 34
NarI GG'CG_CC
Cuts at: 0 675 1304 2022
Size: 675 629 718
Fragments arranged by size:
718 675 629
NcoI C'CATG_G
Cuts at: 0 83 1286 1526 1640 2022
Size: 83 1203 240 114 382
Fragments arranged by size:
1203 382 240 114 83
NspI r_CATG'y
Cuts at: 0 649 2022
Size: 649 1373
PasI CC'CwG_GG
Cuts at: 0 1215 1849 2022
Size: 1215 634 173
Fragments arranged by size:
1215 634 173
PflMI CCAn_nnn'nTGG
Cuts at: 0 1721 2022
Size: 1721 301
PfoI T'CCnGG_A
Cuts at: 0 386 1832 2022
Size: 386 1446 190
Fragments arranged by size:
1446 386 190
PmlI CAC'GTG
Cuts at: 0 1326 1361 2022
Size: 1326 35 661
Fragments arranged by size:
1326 661 35
PpuMI rG'GwC_Cy
Cuts at: 0 300 2022
Size: 300 1722
PshAI GACnn'nnGTC
Cuts at: 0 139 313 2022
Size: 139 174 1709
Fragments arranged by size:
1709 174 139
PspOMI G'GGCC_C
Cuts at: 0 1074 2022
Size: 1074 948
PstI C_TGCA'G
Cuts at: 0 266 404 818 833 1859 2022
Size: 266 138 414 15 1026 163
Fragments arranged by size:
1026 414 266 163 138 15
PvuII CAG'CTG
Cuts at: 0 966 2022
Size: 966 1056
SacI G_AGCT'C
Cuts at: 0 758 2022
Size: 758 1264
SacII CC_GC'GG
Cuts at: 0 933 1845 2022
Size: 933 912 177
Fragments arranged by size:
933 912 177
SapI GCTCTTCn'nnn_
Cuts at: 0 550 2022
Size: 550 1472
SbfI CC_TGCA'GG
Cuts at: 0 404 833 2022
Size: 404 429 1189
Fragments arranged by size:
1189 429 404
SfcI C'TryA_G
Cuts at: 0 262 400 739 814 829 1855 2022
Size: 262 138 339 75 15 1026 167
Fragments arranged by size:
1026 339 262 167 138 75 15
SfoI GGC'GCC
Cuts at: 0 676 1305 2022
Size: 676 629 717
Fragments arranged by size:
717 676 629
SmaI CCC'GGG
Cuts at: 0 1756 2022
Size: 1756 266
SmlI C'TyrA_G
Cuts at: 0 304 535 1234 2022
Size: 304 231 699 788
Fragments arranged by size:
788 699 304 231
SnaBI TAC'GTA
Cuts at: 0 1548 2022
Size: 1548 474
SphI G_CATG'C
Cuts at: 0 649 2022
Size: 649 1373
StyI C'CwwG_G
Cuts at: 0 83 311 985 1286 1448 1526 1574 1640
Size: 83 228 674 301 162 78 48 66
Cuts at: 1640 2022
Size: 382
Fragments arranged by size:
674 382 301 228 162 83 78 66
48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1014 2022
Size: 1014 1008
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 81 435 2022
Size: 81 354 1587
Fragments arranged by size:
1587 354 81
XmaI C'CCGG_G
Cuts at: 0 1754 2022
Size: 1754 268
Enzymes that do cut:
AarI AcuI AleI AlfI AloI AlwNI ApaI ApaLI
AvaI BanI BanII BbeI BbsI BglI Bme1580I BmrI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI
BsrDI BstAPI BstEII BstXI BtgI BtgZI BtsI EaeI
EagI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI
FspI FspAI HaeII Hin4I HincII KasI MmeI MscI
MslI MspA1I NarI NcoI NspI PasI PflMI PfoI
PmlI PpuMI PshAI PspOMI PstI PvuII SacI SacII
SapI SbfI SfcI SfoI SmaI SmlI SnaBI SphI
StyI TaqII XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmgBI
BmtI BplI BsaBI BsaWI BsiWI BsmI BspEI BspHI
BsrFI BsrGI BssHII BssSI BstBI BstYI BstZ17I Bsu36I
ClaI CspCI DraI DraIII DrdI EciI EcoRI FseI
HindIII HpaI KpnI MfeI MluI NaeI NdeI NgoMIV
NheI NotI NruI NsiI PacI PciI PmeI PpiI
PsiI PspXI PsrI PvuI RsrII SalI SanDI ScaI
SexAI SfiI SgfI SgrAI SpeI SrfI SspI StuI
SwaI TatI TstI Tth111I XbaI XhoI XmnI ZraI