(Circular) (Six-base) MAPSORT of: pFN21AB3788.seq Check: 6765 from: 1 to: 6847 pFN21AB3788 6847 bp With 182 enzymes: SgfI * February 27, 2009 14:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4657 278 Size: 53 83 186 936 3121 2468 Fragments arranged by size: 3121 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3245 4190 1937 Size: 1308 945 4594 Fragments arranged by size: 4594 1308 945 Acc65I G'GTAC_C Cuts at: 4168 4168 Size: 6847 AclI AA'CG_TT Cuts at: 1563 4974 5347 1563 Size: 3411 373 3063 Fragments arranged by size: 3411 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2358 2573 2881 3736 4910 6294 6562 1632 Size: 726 215 308 855 1174 1384 268 1917 Fragments arranged by size: 1917 1384 1174 855 726 308 268 215 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6650 AflIII A'CryG_T Cuts at: 1203 5746 6723 1203 Size: 4543 977 1327 Fragments arranged by size: 4543 1327 977 AgeI A'CCGG_T Cuts at: 5678 5678 Size: 6847 AhdI GACnn_n'nnGTC Cuts at: 5576 5576 Size: 6847 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6847 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6847 AlwNI CAG_nnn'CTG Cuts at: 2077 4590 6162 2077 Size: 2513 1572 2762 Fragments arranged by size: 2762 2513 1572 ApaLI G'TGCA_C Cuts at: 4904 6060 4904 Size: 1156 5691 ApoI r'AATT_y Cuts at: 1493 2219 2364 2431 2540 2743 2828 3311 3409 Size: 726 145 67 109 203 85 483 98 Cuts at: 3409 3571 3809 3849 4064 4101 4156 4382 4534 Size: 162 238 40 215 37 55 226 152 Cuts at: 4534 6643 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 726 483 238 226 215 203 162 152 145 109 98 85 67 55 40 37 AseI AT'TA_AT Cuts at: 160 1032 2453 5401 160 Size: 872 1421 2948 1606 Fragments arranged by size: 2948 1606 1421 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6847 AvaI C'yCGr_G Cuts at: 1870 1943 3321 4172 1870 Size: 73 1378 851 4545 Fragments arranged by size: 4545 1378 851 73 BamHI G'GATC_C Cuts at: 4177 4544 4177 Size: 367 6480 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3588 4168 5623 618 Size: 325 217 627 1801 580 1455 1842 Fragments arranged by size: 1842 1801 1455 627 580 325 217 BanII G_rGCy'C Cuts at: 1391 2851 4166 1391 Size: 1460 1315 4072 Fragments arranged by size: 4072 1460 1315 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2295 3889 928 Size: 971 396 1594 3886 Fragments arranged by size: 3886 1594 971 396 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5038 5072 1903 Size: 34 3101 34 3678 Fragments arranged by size: 3678 3101 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2551 4739 5949 2551 Size: 2188 1210 3449 Fragments arranged by size: 3449 2188 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6847 BfrBI ATG'CAT Cuts at: 2634 2753 2634 Size: 119 6728 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5458 136 Size: 107 122 71 631 605 3786 1525 Fragments arranged by size: 3786 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6842 6842 Size: 6847 BlpI GC'TnA_GC Cuts at: 4264 4264 Size: 6847 Bme1580I G_kGCm'C Cuts at: 1790 3591 4908 6064 1790 Size: 1801 1317 1156 2573 Fragments arranged by size: 2573 1801 1317 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6847 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4578 5536 442 Size: 416 53 83 399 24 3161 958 1753 Fragments arranged by size: 3161 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6847 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2269 5507 1239 Size: 1030 3238 2579 Fragments arranged by size: 3238 2579 1030 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4972 5837 6135 4972 Size: 865 298 5684 Fragments arranged by size: 5684 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5510 882 Size: 617 4011 2219 Fragments arranged by size: 4011 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4638 493 Size: 711 3434 2702 Fragments arranged by size: 3434 2702 711 BsaBI GATnn'nnATC Cuts at: 6841 6841 Size: 6847 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4654 5036 5671 275 Size: 53 83 186 936 3121 382 635 1451 Fragments arranged by size: 3121 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5280 5678 5952 6099 6510 5280 Size: 398 274 147 411 5617 Fragments arranged by size: 5617 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6622 Fragments arranged by size: 6622 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2078 2210 2957 1186 Size: 892 132 747 5076 Fragments arranged by size: 5076 892 747 132 BseYI C'CCAG_C Cuts at: 6050 6546 6050 Size: 496 6351 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3074 1163 Size: 573 1338 4936 Fragments arranged by size: 4936 1338 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4297 5058 6086 1540 Size: 275 186 2296 761 1028 2301 Fragments arranged by size: 2301 2296 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4908 4993 6064 4908 Size: 85 1071 5691 Fragments arranged by size: 5691 1071 85 BsmI GAATG_Cn' Cuts at: 2636 2795 4358 4451 2636 Size: 159 1563 93 5032 Fragments arranged by size: 5032 1563 159 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2851 3591 4166 4908 4993 6064 1391 Size: 399 1061 740 575 742 85 1071 2174 Fragments arranged by size: 2174 1071 1061 742 740 575 399 85 BspEI T'CCGG_A Cuts at: 6510 6510 Size: 6847 BspHI T'CATG_A Cuts at: 4736 6466 4736 Size: 1730 5117 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4202 844 Size: 3358 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4734 1394 Size: 429 2911 3507 Fragments arranged by size: 3507 2911 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2012 5342 5516 65 Size: 1183 764 3330 174 1396 Fragments arranged by size: 3330 1396 1183 764 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5491 5678 1700 Size: 110 3681 187 2869 Fragments arranged by size: 3681 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2920 4006 96 Size: 2824 1086 2937 Fragments arranged by size: 2937 2824 1086 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6847 BssSI C'ACGA_G Cuts at: 4907 5919 4907 Size: 1012 5835 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6847 BstBI TT'CG_AA Cuts at: 5651 5651 Size: 6847 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6847 BstXI CCAn_nnnn'nTGG Cuts at: 2247 2247 Size: 6847 BstYI r'GATC_y Cuts at: 1972 4177 4544 4929 4946 6398 6484 6496 6842 Size: 2205 367 385 17 1452 86 12 346 Cuts at: 6842 1972 Size: 1977 Fragments arranged by size: 2205 1977 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2201 513 Size: 552 1136 5159 Fragments arranged by size: 5159 1136 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6108 Fragments arranged by size: 6108 683 56 BtsI GCAGTG_nn' Cuts at: 2800 4363 5157 5177 2800 Size: 1563 794 20 4470 Fragments arranged by size: 4470 1563 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6847 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6812 DraI TTT'AAA Cuts at: 3408 4151 3408 Size: 743 6104 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5854 809 Size: 507 4538 1802 Fragments arranged by size: 4538 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4294 5183 6812 Size: 54 1425 50 275 18 2464 889 1629 Cuts at: 6812 8 Size: 43 Fragments arranged by size: 2464 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4294 1537 Size: 275 2482 4090 Fragments arranged by size: 4090 2482 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2334 3357 3441 3519 3687 3755 4105 4777 Size: 981 1023 84 78 168 68 350 672 Cuts at: 4777 1353 Size: 3423 Fragments arranged by size: 3423 1023 981 672 350 168 84 78 68 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5434 5808 5954 387 Size: 1064 138 3845 374 146 1280 Fragments arranged by size: 3845 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2269 2358 2573 2881 3736 4910 5507 Size: 393 637 89 215 308 855 1174 597 Cuts at: 5507 6294 6562 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1174 855 787 637 597 393 308 268 215 89 EcoO109I rG'GnC_Cy Cuts at: 3117 3117 Size: 6847 EcoRI G'AATT_C Cuts at: 3849 4101 4156 4534 3849 Size: 252 55 378 6162 Fragments arranged by size: 6162 378 252 55 FspI TGC'GCA Cuts at: 5353 5353 Size: 6847 HaeII r_GCGC'y Cuts at: 1678 5994 1678 Size: 4316 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3106 3138 3244 3276 5488 Size: 32 135 32 1534 32 106 32 2212 Cuts at: 5488 5520 5562 5594 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2212 1534 135 106 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2231 4191 4437 669 Size: 988 281 293 1960 246 3079 Fragments arranged by size: 3079 1960 988 293 281 246 HindIII A'AGCT_T Cuts at: 748 3200 748 Size: 2452 4395 HpaI GTT'AAC Cuts at: 4437 4437 Size: 6847 KpnI G_GTAC'C Cuts at: 4172 4172 Size: 6847 MfeI C'AATT_G Cuts at: 4446 4446 Size: 6847 MluI A'CGCG_T Cuts at: 6723 6723 Size: 6847 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2097 2163 5961 6145 1305 Size: 792 66 3798 184 2007 Fragments arranged by size: 3798 2007 792 184 66 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5025 Fragments arranged by size: 5025 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2412 2735 4805 5164 5323 518 Size: 648 1246 323 2070 359 159 2042 Fragments arranged by size: 2070 2042 1246 648 359 323 159 MspA1I CmG'CkG Cuts at: 1606 2077 4263 4940 6088 6333 1606 Size: 471 2186 677 1148 245 2120 Fragments arranged by size: 2186 2120 1148 677 471 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6295 NdeI CA'TA_TG Cuts at: 387 387 Size: 6847 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6847 NotI GC'GGCC_GC Cuts at: 4294 4294 Size: 6847 NsiI A_TGCA'T Cuts at: 2636 2755 2636 Size: 119 6728 NspI r_CATG'y Cuts at: 1132 1229 4205 5750 1132 Size: 97 2976 1545 2229 Fragments arranged by size: 2976 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6847 PciI A'CATG_T Cuts at: 5746 5746 Size: 6847 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6847 PmeI GTTT'AAAC Cuts at: 4151 4151 Size: 6847 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4916 4948 6429 6461 4916 Size: 32 1481 32 5302 Fragments arranged by size: 5302 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6847 PsiI TTA'TAA Cuts at: 3207 4417 3207 Size: 1210 5637 PstI C_TGCA'G Cuts at: 830 4199 830 Size: 3369 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6847 PvuII CAG'CTG Cuts at: 2077 2077 Size: 6847 SalI G'TCGA_C Cuts at: 1936 4189 1936 Size: 2253 4594 SbfI CC_TGCA'GG Cuts at: 4199 4199 Size: 6847 ScaI AGT'ACT Cuts at: 5095 6807 5095 Size: 1712 5135 SfcI C'TryA_G Cuts at: 826 1045 2125 3928 4012 4195 5330 6011 6202 Size: 219 1080 1803 84 183 1135 681 191 Cuts at: 6202 826 Size: 1471 Fragments arranged by size: 1803 1471 1135 1080 681 219 191 183 84 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6847 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6847 SmaI CCC'GGG Cuts at: 1872 4174 1872 Size: 2302 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4951 5852 6114 820 Size: 197 926 3008 901 262 1553 Fragments arranged by size: 3008 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4638 493 Size: 4145 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6847 SphI G_CATG'C Cuts at: 1132 4205 1132 Size: 3073 3774 SspI AAT'ATT Cuts at: 5 52 2584 4771 5 Size: 47 2532 2187 2081 Fragments arranged by size: 2532 2187 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6847 StyI C'CwwG_G Cuts at: 513 1065 4286 513 Size: 552 3221 3074 Fragments arranged by size: 3221 3074 552 SwaI ATTT'AAAT Cuts at: 3408 3408 Size: 6847 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2072 4874 4891 5044 5229 5674 325 Size: 1255 492 2802 17 153 185 445 1498 Fragments arranged by size: 2802 1498 1255 492 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2920 3436 3766 Size: 274 80 33 51 1443 943 516 330 Cuts at: 3766 4006 4126 5093 6805 96 Size: 240 120 967 1712 138 Fragments arranged by size: 1712 1443 967 943 516 330 274 240 138 120 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6807 6839 6807 Size: 32 6815 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6847 XbaI T'CTAG_A Cuts at: 4183 4183 Size: 6847 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6847 XmaI C'CCGG_G Cuts at: 1870 4172 1870 Size: 2302 4545 XmnI GAAnn'nnTTC Cuts at: 2583 2894 3019 3062 3146 3560 3685 3853 3937 Size: 311 125 43 84 414 125 168 84 Cuts at: 3937 4021 4064 4976 2583 Size: 84 43 912 4454 Fragments arranged by size: 4454 912 414 311 168 125 125 84 84 84 43 43 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4655 276 Size: 53 83 186 936 3121 2468 Fragments arranged by size: 3121 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI XcmI