(Circular) (Six-base) MAPSORT of: pFN21AB3698.seq Check: 6221 from: 1 to: 5830 pFN21AB3698 5830 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2604 2604 Size: 5830 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3640 278 Size: 53 83 186 936 2104 2468 Fragments arranged by size: 2468 2104 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3173 1937 Size: 1236 4594 Acc65I G'GTAC_C Cuts at: 3151 3151 Size: 5830 AclI AA'CG_TT Cuts at: 1563 3957 4330 1563 Size: 2394 373 3063 Fragments arranged by size: 3063 2394 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2980 3092 3893 5277 5545 1632 Size: 1348 112 801 1384 268 1917 Fragments arranged by size: 1917 1384 1348 801 268 112 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5633 AflIII A'CryG_T Cuts at: 1203 2424 4729 5706 1203 Size: 1221 2305 977 1327 Fragments arranged by size: 2305 1327 1221 977 AgeI A'CCGG_T Cuts at: 4661 4661 Size: 5830 AhdI GACnn_n'nnGTC Cuts at: 4559 4559 Size: 5830 AleI CACnn'nnGTG Cuts at: 1166 2573 2588 1166 Size: 1407 15 4408 Fragments arranged by size: 4408 1407 15 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5830 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3098 3130 3098 Size: 32 5798 AlwNI CAG_nnn'CTG Cuts at: 3573 5145 3573 Size: 1572 4258 ApaLI G'TGCA_C Cuts at: 2392 2591 2846 3887 5043 2392 Size: 199 255 1041 1156 3179 Fragments arranged by size: 3179 1156 1041 255 199 ApoI r'AATT_y Cuts at: 1493 3034 3139 3365 3517 5626 1493 Size: 1541 105 226 152 2109 1697 Fragments arranged by size: 2109 1697 1541 226 152 105 AseI AT'TA_AT Cuts at: 160 1032 4384 160 Size: 872 3352 1606 Fragments arranged by size: 3352 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5830 AvaI C'yCGr_G Cuts at: 1870 1943 2025 2090 2654 2984 3155 1870 Size: 73 82 65 564 330 171 4545 Fragments arranged by size: 4545 564 330 171 82 73 65 BamHI G'GATC_C Cuts at: 3160 3527 3160 Size: 367 5463 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2054 2120 2575 2742 3151 Size: 325 217 627 267 66 455 167 409 Cuts at: 3151 4606 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 455 409 325 267 217 167 66 BanII G_rGCy'C Cuts at: 1391 2097 2151 3149 1391 Size: 706 54 998 4072 Fragments arranged by size: 4072 998 706 54 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2431 2797 2827 928 Size: 971 532 366 30 3931 Fragments arranged by size: 3931 971 532 366 30 BbvCI CC'TCA_GC Cuts at: 3008 3008 Size: 5830 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2770 2804 4021 4055 1903 Size: 34 833 34 1217 34 3678 Fragments arranged by size: 3678 1217 833 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3722 4932 3722 Size: 1210 4620 BclI T'GATC_A Cuts at: 1548 1548 Size: 5830 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2764 3074 4441 Size: 107 122 71 631 605 1092 310 1367 Cuts at: 4441 136 Size: 1525 Fragments arranged by size: 1525 1367 1092 631 605 310 122 107 71 BglII A'GATC_T Cuts at: 5825 5825 Size: 5830 BlpI GC'TnA_GC Cuts at: 2531 3247 2531 Size: 716 5114 Bme1580I G_kGCm'C Cuts at: 1790 2123 2396 2580 2595 2682 2850 3891 5047 Size: 333 273 184 15 87 168 1041 1156 Cuts at: 5047 1790 Size: 2573 Fragments arranged by size: 2573 1156 1041 333 273 184 168 87 15 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5830 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3561 4519 442 Size: 416 53 83 399 24 2144 958 1753 Fragments arranged by size: 2144 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5830 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2060 4490 1239 Size: 821 2430 2579 Fragments arranged by size: 2579 2430 821 Bpu10I CC'TnA_GC Cuts at: 3008 3008 Size: 5830 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3955 4820 5118 3955 Size: 865 298 4667 Fragments arranged by size: 4667 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2984 4493 882 Size: 617 1485 1509 2219 Fragments arranged by size: 2219 1509 1485 617 BsaAI yAC'GTr Cuts at: 493 1204 2170 3621 493 Size: 711 966 1451 2702 Fragments arranged by size: 2702 1451 966 711 BsaBI GATnn'nnATC Cuts at: 2279 5824 2279 Size: 3545 2285 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3637 4019 4654 275 Size: 53 83 186 936 2104 382 635 1451 Fragments arranged by size: 2104 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4263 4661 4935 5082 5493 4263 Size: 398 274 147 411 4600 Fragments arranged by size: 4600 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2069 2099 2633 2663 1178 Size: 30 165 30 666 30 534 30 4345 Fragments arranged by size: 4345 666 534 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5830 BseYI C'CCAG_C Cuts at: 2665 5033 5529 2665 Size: 2368 496 2966 Fragments arranged by size: 2966 2368 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2345 2666 1163 Size: 573 609 321 4327 Fragments arranged by size: 4327 609 573 321 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2467 3280 4041 5069 1540 Size: 275 186 466 813 761 1028 2301 Fragments arranged by size: 2301 1028 813 761 466 275 186 BsiHKAI G_wGCw'C Cuts at: 2097 2151 2160 2396 2595 2850 3891 3976 5047 Size: 54 9 236 199 255 1041 85 1071 Cuts at: 5047 2097 Size: 2880 Fragments arranged by size: 2880 1071 1041 255 236 199 85 54 9 BsmI GAATG_Cn' Cuts at: 3341 3434 3341 Size: 93 5737 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2097 2123 2151 2160 2396 2580 2595 Size: 399 307 26 28 9 236 184 15 Cuts at: 2595 2682 2850 3149 3891 3976 5047 1391 Size: 87 168 299 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 307 299 236 184 168 87 85 28 26 15 9 BspEI T'CCGG_A Cuts at: 5493 5493 Size: 5830 BspHI T'CATG_A Cuts at: 3719 5449 3719 Size: 1730 4100 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2367 2407 2604 3185 844 Size: 1523 40 197 581 3489 Fragments arranged by size: 3489 1523 581 197 40 BsrBI CCG'CTC Cuts at: 1394 1823 2113 2368 2879 3717 1394 Size: 429 290 255 511 838 3507 Fragments arranged by size: 3507 838 511 429 290 255 BsrDI GCAATG_nn' Cuts at: 65 1248 4325 4499 65 Size: 1183 3077 174 1396 Fragments arranged by size: 3077 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2739 3040 4474 4661 1700 Size: 110 929 301 1434 187 2869 Fragments arranged by size: 2869 1434 929 301 187 110 BsrGI T'GTAC_A Cuts at: 96 2427 3015 96 Size: 2331 588 2911 Fragments arranged by size: 2911 2331 588 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5830 BssSI C'ACGA_G Cuts at: 2188 2363 3890 4902 2188 Size: 175 1527 1012 3116 Fragments arranged by size: 3116 1527 1012 175 BstAPI GCAn_nnn'nTGC Cuts at: 794 2854 794 Size: 2060 3770 BstBI TT'CG_AA Cuts at: 4634 4634 Size: 5830 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5830 BstYI r'GATC_y Cuts at: 1972 3160 3527 3912 3929 5381 5467 5479 5825 Size: 1188 367 385 17 1452 86 12 346 Cuts at: 5825 1972 Size: 1977 Fragments arranged by size: 1977 1452 1188 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2746 3126 513 Size: 552 939 742 380 3217 Fragments arranged by size: 3217 939 742 552 380 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2365 2458 487 Size: 683 56 1139 93 3859 Fragments arranged by size: 3859 1139 683 93 56 BtsI GCAGTG_nn' Cuts at: 2654 3127 3346 4140 4160 2654 Size: 473 219 794 20 4324 Fragments arranged by size: 4324 794 473 219 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5830 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5795 DraI TTT'AAA Cuts at: 3134 3134 Size: 5830 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2384 4837 809 Size: 507 1068 2453 1802 Fragments arranged by size: 2453 1802 1068 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2130 2331 2481 Size: 54 1425 50 275 18 300 201 150 Cuts at: 2481 3277 4166 5795 8 Size: 796 889 1629 43 Fragments arranged by size: 1629 1425 889 796 300 275 201 150 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3277 1537 Size: 275 1465 4090 Fragments arranged by size: 4090 1465 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2216 2414 3760 1353 Size: 863 198 1346 3423 Fragments arranged by size: 3423 1346 863 198 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2330 2888 4417 4791 4937 387 Size: 1064 138 741 558 1529 374 146 1280 Fragments arranged by size: 1529 1280 1064 741 558 374 146 138 EcoICRI GAG'CTC Cuts at: 2095 2149 2095 Size: 54 5776 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2060 2980 3092 3893 4490 5277 5545 Size: 393 428 920 112 801 597 787 268 Cuts at: 5545 1239 Size: 1524 Fragments arranged by size: 1524 920 801 787 597 428 393 268 112 EcoNI CCTnn'n_nnAGG Cuts at: 2345 2345 Size: 5830 EcoRI G'AATT_C Cuts at: 3139 3517 3139 Size: 378 5452 FspI TGC'GCA Cuts at: 4336 4336 Size: 5830 HaeII r_GCGC'y Cuts at: 1678 2777 4977 1678 Size: 1099 2200 2531 Fragments arranged by size: 2531 2200 1099 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4471 4503 4545 4577 1373 Size: 32 135 32 2899 32 42 32 2626 Fragments arranged by size: 2899 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2422 2932 3174 3420 669 Size: 988 281 484 510 242 246 3079 Fragments arranged by size: 3079 988 510 484 281 246 242 HindIII A'AGCT_T Cuts at: 748 748 Size: 5830 HpaI GTT'AAC Cuts at: 3420 3420 Size: 5830 KpnI G_GTAC'C Cuts at: 3155 3155 Size: 5830 MfeI C'AATT_G Cuts at: 3429 3429 Size: 5830 MluI A'CGCG_T Cuts at: 5706 5706 Size: 5830 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2973 3084 4944 5128 1305 Size: 1668 111 1860 184 2007 Fragments arranged by size: 2007 1860 1668 184 111 MscI TGG'CCA Cuts at: 10 64 1489 1832 2132 2483 10 Size: 54 1425 343 300 351 3357 Fragments arranged by size: 3357 1425 351 343 300 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2327 2348 2399 2453 2549 2573 Size: 648 843 318 21 51 54 96 24 Cuts at: 2573 2588 2843 3788 4147 4306 518 Size: 15 255 945 359 159 2042 Fragments arranged by size: 2042 945 843 648 359 318 255 159 96 54 51 24 21 15 MspA1I CmG'CkG Cuts at: 1606 2860 2955 3246 3923 5071 5316 1606 Size: 1254 95 291 677 1148 245 2120 Fragments arranged by size: 2120 1254 1148 677 291 245 95 NaeI GCC'GGC Cuts at: 3042 3042 Size: 5830 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4339 Fragments arranged by size: 4339 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5830 NgoMIV G'CCGG_C Cuts at: 3040 3040 Size: 5830 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5830 NotI GC'GGCC_GC Cuts at: 3277 3277 Size: 5830 NspI r_CATG'y Cuts at: 1132 1229 2428 3188 4733 1132 Size: 97 1199 760 1545 2229 Fragments arranged by size: 2229 1545 1199 760 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5830 PciI A'CATG_T Cuts at: 2424 4729 2424 Size: 2305 3525 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5830 PfoI T'CCnGG_A Cuts at: 2637 2637 Size: 5830 PmeI GTTT'AAAC Cuts at: 3134 3134 Size: 5830 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3899 3931 5412 5444 3899 Size: 32 1481 32 4285 Fragments arranged by size: 4285 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5830 PsiI TTA'TAA Cuts at: 3400 3400 Size: 5830 PspXI vC'TCGA_Gb Cuts at: 2654 2654 Size: 5830 PstI C_TGCA'G Cuts at: 830 2418 2501 2601 2859 3182 830 Size: 1588 83 100 258 323 3478 Fragments arranged by size: 3478 1588 323 258 100 83 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5830 PvuII CAG'CTG Cuts at: 2955 2955 Size: 5830 SacI G_AGCT'C Cuts at: 2097 2151 2097 Size: 54 5776 SalI G'TCGA_C Cuts at: 1936 3172 1936 Size: 1236 4594 SapI GCTCTTCn'nnn_ Cuts at: 2414 2414 Size: 5830 SbfI CC_TGCA'GG Cuts at: 2418 3182 2418 Size: 764 5066 ScaI AGT'ACT Cuts at: 4078 5790 4078 Size: 1712 4118 SfcI C'TryA_G Cuts at: 826 1045 2414 2497 2597 2855 2974 3178 4313 Size: 219 1369 83 100 258 119 204 1135 Cuts at: 4313 4994 5185 826 Size: 681 191 1471 Fragments arranged by size: 1471 1369 1135 681 258 219 204 191 119 100 83 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5830 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5830 SmaI CCC'GGG Cuts at: 1872 2027 3157 1872 Size: 155 1130 4545 Fragments arranged by size: 4545 1130 155 SmlI C'TyrA_G Cuts at: 820 1017 1943 2654 3934 4835 5097 820 Size: 197 926 711 1280 901 262 1553 Fragments arranged by size: 1553 1280 926 901 711 262 197 SnaBI TAC'GTA Cuts at: 493 3621 493 Size: 3128 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5830 SphI G_CATG'C Cuts at: 1132 3188 1132 Size: 2056 3774 SspI AAT'ATT Cuts at: 5 52 3754 5 Size: 47 3702 2081 Fragments arranged by size: 3702 2081 47 StuI AGG'CCT Cuts at: 1517 2615 1517 Size: 1098 4732 StyI C'CwwG_G Cuts at: 513 1065 2004 3269 513 Size: 552 939 1265 3074 Fragments arranged by size: 3074 1265 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3857 3874 4027 4212 4657 325 Size: 1255 2277 17 153 185 445 1498 Fragments arranged by size: 2277 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2292 2427 3015 Size: 274 80 33 51 1443 315 135 588 Cuts at: 3015 4076 5788 96 Size: 1061 1712 138 Fragments arranged by size: 1712 1443 1061 588 315 274 138 135 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2633 2665 5790 5822 2633 Size: 32 3125 32 2641 Fragments arranged by size: 3125 2641 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5830 XbaI T'CTAG_A Cuts at: 3166 3166 Size: 5830 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2224 2224 Size: 5830 XhoI C'TCGA_G Cuts at: 1943 2654 1943 Size: 711 5119 XmaI C'CCGG_G Cuts at: 1870 2025 3155 1870 Size: 155 1130 4545 Fragments arranged by size: 4545 1130 155 XmnI GAAnn'nnTTC Cuts at: 2834 3053 3959 2834 Size: 219 906 4705 Fragments arranged by size: 4705 906 219 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3638 276 Size: 53 83 186 936 2104 2468 Fragments arranged by size: 2468 2104 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoO109I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspOMI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI