Restriction map for
pFN21AB3640
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# NO CUT ENZYMES ( 138 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI BalI BbrPI
BbsI BcgI BciVI BfrI BglI
BlpI Bpu1102I BpuAI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmBI BsmFI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoT22I FseI HgaI
HincII HindII HindIII HpaI KpnI
KspI MfeI MluI MluNI MroI
MscI MunI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfaNI SfiI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI SwaI TaqI
Tth111I TthHB8I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AcsI 875 GAATTC [GA]^AATT[CT]
AcyI 151 GGCGCC G[GA]^CG[CT]C
ApoI 875 GAATTC [GA]^AATT[CT]
AvaI 416 CCCGAG C^[CT]CG[GA]G
AviII 1152 TGCGCA TGC^GCA
AvrII 681 CCTAGG C^CTAGG
BamHI 7 GGATCC G^GATCC
BanI 151 GGCGCC G^G[CT][GA]CC
BanII 593 GGGCTC G[GA]GC[CT]^C
BbeI 151 GGCGCC GGCGC^C
BbvCI 659 CCTCAGC CCTCAGC(-5/-2)
BclI 774 TGATCA T^GATCA
BfaI 682 CTAG C^TAG
BlnI 681 CCTAGG C^CTAGG
BsaAI 482 CACGTA [CT]AC^GT[GA]
BsaBI 991 GATGATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 151 GGCGCC G[GA]^CG[CT]C
BsaI 833 GAGACC GGTCTC(1/5)
BsmAI 833 GAGAC GTCTC(1/5)
BsoBI 416 CCCGAG C^[CT]CG[GA]G
BspMI 69 GCAGGT ACCTGC(4/8)
BstAPI 162 GCAGTTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraI 517 TTTAAA TTT^AAA
DraII 431 AGGCCCT [GA]G^G[ACGT]CC[CT]
EarI 1009 GAAGAG CTCTTC(1/4)
EcoO109I 431 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRI 875 GAATTC G^AATTC
EcoRV 1235 GATATC GAT^ATC
EcoT14I 681 CCTAGG C^C[AT][AT]GG
FbaI 774 TGATCA T^GATCA
FspI 1152 TGCGCA TGC^GCA
Hin1I 151 GGCGCC G[GA]^CG[CT]C
KasI 151 GGCGCC G^GCGCC
Ksp632I 1009 GAAGAG CTCTTC(1/4)
MaeI 682 CTAG C^TAG
MaeII 483 ACGT A^CGT
MamI 991 GATGATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 518 TTAA T^TAA
NarI 151 GGCGCC GG^CGCC
NspI 848 GCATGC [GA]CATG^[CT]
PstI 637 CTGCAG CTGCA^G
PvuII 662 CAGCTG CAG^CTG
SfcI 637 CTGCAG C^T[GA][CT]AG
SfoI 151 GGCGCC GGC^GCC
SphI 848 GCATGC GCATG^C
StuI 50 AGGCCT AGG^CCT
StyI 681 CCTAGG C^C[AT][AT]GG
Tru9I 518 TTAA T^TAA
XcmI 788 CCAGTAAAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccII 27 CGCG CG^CG
38 CGCG
AfaI 73 GTAC GT^AC
656 GTAC
AvaII 471 GGACC G^G[AT]CC
1063 GGACC
BcnI 148 CCCGG CC^[GC]GG
185 CCGGG
BglII 736 AGATCT A^GATCT
939 AGATCT
BmyI 413 GTGCCC G[AGT]GC[ACT]^C
593 GGGCTC
BseRI 96 CTCCTC GAGGAG(10/8)
1045 GAGGAG
BsgI 500 GTGCAG GTGCAG(16/14)
897 GTGCAG
BsmI 804 GAATGC GAATGC(1/-1)
914 GCATTC
Bsp1286I 413 GTGCCC G[AGT]GC[ACT]^C
593 GGGCTC
BspHI 450 TCATGA T^CATGA
855 TCATGA
BstUI 27 CGCG CG^CG
38 CGCG
EcoNI 1099 CCTGGCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1223 CCTACAAGAGG
FauI 1212 CCCGC CCCGC(4/6)
4 GCGGG
HaeII 151 GGCGCC [GA]GCGC^[CT]
234 GGCGCT
HinfI 686 GAATC G^A[ACGT]TC
1141 GATTC
MslI 393 CATGTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
957 CACTCCAGTG
MspA1I 662 CAGCTG C[AC]G^C[GT]G
1213 CCGCTG
MvnI 27 CGCG CG^CG
38 CGCG
NciI 148 CCCGG CC^[GC]GG
185 CCGGG
RcaI 450 TCATGA T^CATGA
855 TCATGA
RsaI 73 GTAC GT^AC
656 GTAC
SmlI 157 CTCAAG C^T[CT][GA]AG
1000 CTTGAG
TfiI 686 GAATC G^A[AT]TC
1141 GATTC
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# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AluI AlwI BbvI BpmI
BsaJI BsiYI BslI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII EcoRII Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HpaII HphI ItaI MaeIII MboI
MboII MflI MnlI MspI MvaI
MwoI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI TseI Tsp45I Tsp509I
TspRI XhoII