(Circular) (Six-base) MAPSORT of: pFN21AB3640.seq Check: 4679 from: 1 to: 5962 pFN21AB3640 5962 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3772 278 Size: 53 83 186 936 2236 2468 Fragments arranged by size: 2468 2236 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3305 1937 Size: 1368 4594 Acc65I G'GTAC_C Cuts at: 3283 3283 Size: 5962 AclI AA'CG_TT Cuts at: 1563 4089 4462 1563 Size: 2526 373 3063 Fragments arranged by size: 3063 2526 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4025 5409 5677 1632 Size: 2393 1384 268 1917 Fragments arranged by size: 2393 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5765 AflIII A'CryG_T Cuts at: 1203 4861 5838 1203 Size: 3658 977 1327 Fragments arranged by size: 3658 1327 977 AgeI A'CCGG_T Cuts at: 4793 4793 Size: 5962 AhdI GACnn_n'nnGTC Cuts at: 4691 4691 Size: 5962 AleI CACnn'nnGTG Cuts at: 1166 2966 1166 Size: 1800 4162 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5962 AlwNI CAG_nnn'CTG Cuts at: 3705 5277 3705 Size: 1572 4390 ApaLI G'TGCA_C Cuts at: 4019 5175 4019 Size: 1156 4806 ApoI r'AATT_y Cuts at: 1493 2880 3271 3497 3649 5758 1493 Size: 1387 391 226 152 2109 1697 Fragments arranged by size: 2109 1697 1387 391 226 152 AseI AT'TA_AT Cuts at: 160 1032 4516 160 Size: 872 3484 1606 Fragments arranged by size: 3484 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5962 AvaI C'yCGr_G Cuts at: 1870 1943 2421 3287 1870 Size: 73 478 866 4545 Fragments arranged by size: 4545 866 478 73 AvrII C'CTAG_G Cuts at: 2686 2686 Size: 5962 BamHI G'GATC_C Cuts at: 2012 3292 3659 2012 Size: 1280 367 4315 Fragments arranged by size: 4315 1280 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2156 3283 4738 618 Size: 325 217 627 369 1127 1455 1842 Fragments arranged by size: 1842 1455 1127 627 369 325 217 BanII G_rGCy'C Cuts at: 1391 2602 3281 1391 Size: 1211 679 4072 Fragments arranged by size: 4072 1211 679 BbeI G_GCGC'C Cuts at: 2160 2160 Size: 5962 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4991 BbvCI CC'TCA_GC Cuts at: 2665 2665 Size: 5962 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4153 4187 1903 Size: 34 2216 34 3678 Fragments arranged by size: 3678 2216 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3854 5064 3854 Size: 1210 4752 BclI T'GATC_A Cuts at: 1548 2779 1548 Size: 1231 4731 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4573 136 Size: 107 122 71 631 605 2901 1525 Fragments arranged by size: 2901 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2741 2944 5957 2741 Size: 203 3013 2746 Fragments arranged by size: 3013 2746 203 BlpI GC'TnA_GC Cuts at: 3379 3379 Size: 5962 Bme1580I G_kGCm'C Cuts at: 1790 2422 4023 5179 1790 Size: 632 1601 1156 2573 Fragments arranged by size: 2573 1601 1156 632 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5962 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3693 4651 442 Size: 416 53 83 399 24 2276 958 1753 Fragments arranged by size: 2276 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5962 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2072 2586 2949 4622 1239 Size: 833 514 363 1673 2579 Fragments arranged by size: 2579 1673 833 514 363 Bpu10I CC'TnA_GC Cuts at: 2665 2665 Size: 5962 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2147 3026 4087 4952 5250 2147 Size: 879 1061 865 298 2859 Fragments arranged by size: 2859 1061 879 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2832 4625 882 Size: 617 1333 1793 2219 Fragments arranged by size: 2219 1793 1333 617 BsaAI yAC'GTr Cuts at: 493 1204 2489 3753 493 Size: 711 1285 1264 2702 Fragments arranged by size: 2702 1285 1264 711 BsaBI GATnn'nnATC Cuts at: 3000 5956 3000 Size: 2956 3006 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2157 3769 4151 4786 Size: 53 83 186 936 624 1612 382 635 Cuts at: 4786 275 Size: 1451 Fragments arranged by size: 1612 1451 936 635 624 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4395 4793 5067 5214 5625 4395 Size: 398 274 147 411 4732 Fragments arranged by size: 4732 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5737 Fragments arranged by size: 5737 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2092 3065 1186 Size: 906 973 4083 Fragments arranged by size: 4083 973 906 BseYI C'CCAG_C Cuts at: 5165 5661 5165 Size: 496 5466 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2526 2923 1163 Size: 573 790 397 4202 Fragments arranged by size: 4202 790 573 397 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3412 4173 5201 1540 Size: 275 186 1411 761 1028 2301 Fragments arranged by size: 2301 1411 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4023 4108 5179 4023 Size: 85 1071 4806 Fragments arranged by size: 4806 1071 85 BsmI GAATG_Cn' Cuts at: 2815 2919 3473 3566 2815 Size: 104 554 93 5211 Fragments arranged by size: 5211 554 104 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2422 2602 3281 4023 4108 5179 1391 Size: 399 632 180 679 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 679 632 399 180 85 BspEI T'CCGG_A Cuts at: 5625 5625 Size: 5962 BspHI T'CATG_A Cuts at: 2455 2860 3851 5581 2455 Size: 405 991 1730 2836 Fragments arranged by size: 2836 1730 991 405 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2065 3317 844 Size: 1221 1252 3489 Fragments arranged by size: 3489 1252 1221 BsrBI CCG'CTC Cuts at: 1394 1823 3849 1394 Size: 429 2026 3507 Fragments arranged by size: 3507 2026 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4457 4631 65 Size: 1183 3209 174 1396 Fragments arranged by size: 3209 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4606 4793 1700 Size: 110 2796 187 2869 Fragments arranged by size: 2869 2796 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5962 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5962 BssSI C'ACGA_G Cuts at: 4022 5034 4022 Size: 1012 4950 BstAPI GCAn_nnn'nTGC Cuts at: 794 2173 794 Size: 1379 4583 BstBI TT'CG_AA Cuts at: 4766 4766 Size: 5962 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5962 BstYI r'GATC_y Cuts at: 1972 2012 2741 2944 3292 3659 4044 4061 5513 Size: 40 729 203 348 367 385 17 1452 Cuts at: 5513 5599 5611 5957 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 729 385 367 348 346 203 86 40 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4471 Fragments arranged by size: 4471 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5223 Fragments arranged by size: 5223 683 56 BtsI GCAGTG_nn' Cuts at: 2808 3478 4272 4292 2808 Size: 670 794 20 4478 Fragments arranged by size: 4478 794 670 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5962 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5927 DraI TTT'AAA Cuts at: 2524 3266 2524 Size: 742 5220 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4969 809 Size: 507 3653 1802 Fragments arranged by size: 3653 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3409 4298 5927 Size: 54 1425 50 275 18 1579 889 1629 Cuts at: 5927 8 Size: 43 Fragments arranged by size: 1629 1579 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3409 1537 Size: 275 1597 4090 Fragments arranged by size: 4090 1597 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3009 3892 1353 Size: 1656 883 3423 Fragments arranged by size: 3423 1656 883 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2095 4549 4923 5069 387 Size: 1064 138 506 2454 374 146 1280 Fragments arranged by size: 2454 1280 1064 506 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2072 2586 2949 4025 4622 5409 5677 Size: 393 440 514 363 1076 597 787 268 Cuts at: 5677 1239 Size: 1524 Fragments arranged by size: 1524 1076 787 597 514 440 393 363 268 EcoNI CCTnn'n_nnAGG Cuts at: 3108 3232 3108 Size: 124 5838 EcoO109I rG'GnC_Cy Cuts at: 2437 2437 Size: 5962 EcoRI G'AATT_C Cuts at: 2880 3271 3649 2880 Size: 391 378 5193 Fragments arranged by size: 5193 391 378 EcoRV GAT'ATC Cuts at: 3242 3242 Size: 5962 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2188 2188 Size: 5962 FspI TGC'GCA Cuts at: 3159 4468 3159 Size: 1309 4653 HaeII r_GCGC'y Cuts at: 1678 2160 2243 5109 1678 Size: 482 83 2866 2531 Fragments arranged by size: 2866 2531 482 83 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2829 2861 3102 3134 3226 Size: 32 135 32 1257 32 241 32 92 Cuts at: 3226 3258 4603 4635 4677 4709 1373 Size: 32 1345 32 42 32 2626 Fragments arranged by size: 2626 1345 1257 241 135 92 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3306 3552 669 Size: 988 281 1368 246 3079 Fragments arranged by size: 3079 1368 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5962 HpaI GTT'AAC Cuts at: 3552 3552 Size: 5962 KasI G'GCGC_C Cuts at: 2156 2156 Size: 5962 KpnI G_GTAC'C Cuts at: 3287 3287 Size: 5962 MfeI C'AATT_G Cuts at: 3561 3561 Size: 5962 MluI A'CGCG_T Cuts at: 5838 5838 Size: 5962 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5076 5260 1305 Size: 3771 184 2007 Fragments arranged by size: 3771 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4140 Fragments arranged by size: 4140 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2402 2966 3920 4279 4438 518 Size: 648 1236 564 954 359 159 2042 Fragments arranged by size: 2042 1236 954 648 564 359 159 MspA1I CmG'CkG Cuts at: 1606 2669 3220 3378 4055 5203 5448 1606 Size: 1063 551 158 677 1148 245 2120 Fragments arranged by size: 2120 1148 1063 677 551 245 158 NarI GG'CG_CC Cuts at: 2157 2157 Size: 5962 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4471 Fragments arranged by size: 4471 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5962 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5962 NotI GC'GGCC_GC Cuts at: 3409 3409 Size: 5962 NspI r_CATG'y Cuts at: 1132 1229 2857 3320 4865 1132 Size: 97 1628 463 1545 2229 Fragments arranged by size: 2229 1628 1545 463 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5962 PciI A'CATG_T Cuts at: 4861 4861 Size: 5962 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5962 PfoI T'CCnGG_A Cuts at: 2343 2343 Size: 5962 PmeI GTTT'AAAC Cuts at: 3266 3266 Size: 5962 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4031 4063 5544 5576 4031 Size: 32 1481 32 4417 Fragments arranged by size: 4417 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5962 PsiI TTA'TAA Cuts at: 3532 3532 Size: 5962 PstI C_TGCA'G Cuts at: 830 2646 3314 830 Size: 1816 668 3478 Fragments arranged by size: 3478 1816 668 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5962 PvuII CAG'CTG Cuts at: 2669 2669 Size: 5962 SalI G'TCGA_C Cuts at: 1936 3304 1936 Size: 1368 4594 SbfI CC_TGCA'GG Cuts at: 3314 3314 Size: 5962 ScaI AGT'ACT Cuts at: 4210 5922 4210 Size: 1712 4250 SfcI C'TryA_G Cuts at: 826 1045 2642 3310 4445 5126 5317 826 Size: 219 1597 668 1135 681 191 1471 Fragments arranged by size: 1597 1471 1135 681 668 219 191 SfoI GGC'GCC Cuts at: 2158 2158 Size: 5962 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5962 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5962 SmaI CCC'GGG Cuts at: 1872 3289 1872 Size: 1417 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2162 3005 4066 4967 5229 820 Size: 197 926 219 843 1061 901 262 1553 Fragments arranged by size: 1553 1061 926 901 843 262 219 197 SnaBI TAC'GTA Cuts at: 493 3753 493 Size: 3260 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5962 SphI G_CATG'C Cuts at: 1132 2857 3320 1132 Size: 1725 463 3774 Fragments arranged by size: 3774 1725 463 SspI AAT'ATT Cuts at: 5 52 3886 5 Size: 47 3834 2081 Fragments arranged by size: 3834 2081 47 StuI AGG'CCT Cuts at: 1517 2057 1517 Size: 540 5422 StyI C'CwwG_G Cuts at: 513 1065 2004 2686 3401 513 Size: 552 939 682 715 3074 Fragments arranged by size: 3074 939 715 682 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3989 4006 4159 4344 4789 325 Size: 1255 2409 17 153 185 445 1498 Fragments arranged by size: 2409 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4208 5920 96 Size: 274 80 33 51 1443 2231 1712 138 Fragments arranged by size: 2231 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5922 5954 5922 Size: 32 5930 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5962 XbaI T'CTAG_A Cuts at: 3298 3298 Size: 5962 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2800 2800 Size: 5962 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5962 XmaI C'CCGG_G Cuts at: 1870 3287 1870 Size: 1417 4545 XmnI GAAnn'nnTTC Cuts at: 4091 4091 Size: 5962 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3770 276 Size: 53 83 186 936 2236 2468 Fragments arranged by size: 2468 2236 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI