Restriction map for
pFN21AB3482
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AviII AvrII BamHI BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrGI BssHII BssSI Bst1107I
BstAPI BstEII BstPI BstUI BstZ17I
Bsu36I CelII CfoI ClaI CpoI
DraII DraIII EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRV FauI FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MunI MvnI NarI NdeI
NheI NotI NruI PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI PvuI RsrII
SacII SalI SapI ScaI SexAI
SfiI SfoI SgrAI SmaI SmlI
SnaBI SpeI SphI SspBI StuI
SwaI Van91I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AciI 1755 CCGC CCGC(-3/-1)
AclI 870 AACGTT AA^CGTT
AlwI 1824 GATCC GGATC(4/5)
AlwNI 1387 CAGATACTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 1368 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 386 GAGCTC G[AT]GC[AT]^C
AspI 1058 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1191 CCCGAG C^[CT]CG[GA]G
BalI 575 TGGCCA TGG^CCA
BanI 2064 GGCACC G^G[CT][GA]CC
BcnI 2084 CCGGG CC^[GC]GG
BfaI 823 CTAG C^TAG
BglI 1648 GCCACAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2095 AGATCT A^GATCT
BsaI 667 GAGACC GGTCTC(1/5)
BsgI 332 CTGCAC GTGCAG(16/14)
BsiHKAI 386 GAGCTC G[AT]GC[AT]^C
BsmFI 1051 GGGAC GGGAC(10/14)
BsmI 1803 GAATGC GAATGC(1/-1)
BsoBI 1191 CCCGAG C^[CT]CG[GA]G
BsrFI 481 GCCGGC [GA]^CCGG[CT]
BsrI 622 CCAGT ACTGG(1/-1)
BstBI 217 TTCGAA TT^CGAA
BstXI 491 CCACAGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 481 GCCGGC [GA]^CCGG[CT]
DrdI 522 GACCTCATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 497 CCATGG C^C[GA][CT]GG
EaeI 575 TGGCCA [CT]^GGCC[GA]
EarI 133 GAAGAG CTCTTC(1/4)
EcoRI 1945 GAATTC G^AATTC
EcoT14I 497 CCATGG C^C[AT][AT]GG
EcoT22I 1156 ATGCAT ATGCA^T
HindIII 941 AAGCTT A^AGCTT
Ksp632I 133 GAAGAG CTCTTC(1/4)
MaeI 823 CTAG C^TAG
MluNI 575 TGGCCA TGG^CCA
MscI 575 TGGCCA TGG^CCA
MspA1I 1442 CAGCTG C[AC]G^C[GT]G
NaeI 481 GCCGGC GCC^GGC
NciI 2084 CCGGG CC^[GC]GG
NcoI 497 CCATGG C^CATGG
NgoMIV 481 GCCGGC G^CCGGC
NsiI 1156 ATGCAT ATGCA^T
NspI 485 GCATGT [GA]CATG^[CT]
NspV 217 TTCGAA TT^CGAA
PflFI 1058 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 870 AACGTT AA^CGTT
PstI 473 CTGCAG CTGCA^G
PvuII 1442 CAGCTG CAG^CTG
SacI 386 GAGCTC GAGCT^C
SbfI 472 CCTGCAGG CCTGCA^GG
SfuI 217 TTCGAA TT^CGAA
Sse8387I 472 CCTGCAGG CCTGCA^GG
StyI 497 CCATGG C^C[AT][AT]GG
Tth111I 1058 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 1368 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
BanII 386 GAGCTC G[GA]GC[CT]^C
1618 GAGCCC
BbvI 347 GCAGC GCAGC(8/12)
1490 GCAGC
BseRI 1295 CTCCTC GAGGAG(10/8)
1463 CTCCTC
BsmAI 667 GAGAC GTCTC(1/5)
818 GTCTC
BspHI 1376 TCATGA T^CATGA
1424 TCATGA
BspMI 330 ACCTGC ACCTGC(4/8)
2050 ACCTGC
BsrDI 1731 CATTGC GCAATG(2/0)
1757 GCAATG
BstYI 1823 AGATCC [GA]^GATC[CT]
2095 AGATCT
HapII 482 CCGG C^CGG
2084 CCGG
HpaII 482 CCGG C^CGG
2084 CCGG
MaeII 871 ACGT A^CGT
1507 ACGT
MflI 1823 AGATCC [GA]^GATC[CT]
2095 AGATCT
MspI 482 CCGG C^CGG
2084 CCGG
RcaI 1376 TCATGA T^CATGA
1424 TCATGA
SfcI 473 CTGCAG C^T[GA][CT]AG
704 CTACAG
SspI 952 AATATT AAT^ATT
1693 AATATT
TaqI 218 TCGA T^CGA
1113 TCGA
TseI 347 GCAGC G^C[AT]GC
1490 GCAGC
TthHB8I 218 TCGA T^CGA
1113 TCGA
XhoII 1823 AGATCC [GA]^GATC[CT]
2095 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AcsI AfaI AluI ApoI AvaII
BbsI BmyI BpmI BpuAI BsaJI
BsiYI BslI Bsp1286I BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DraI EcoRII Fnu4HI FokI HaeIII
HinfI HphI ItaI MaeIII MboI
MboII MnlI MseI MslI MvaI
MwoI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
TfiI Tru9I Tsp45I Tsp509I TspRI