Restriction map for
pFN21AB3482
(Circular) (Six-base) MAPSORT of: pFN21AB3482.seq Check: 4789 from: 1 to: 6904
pFN21AB3482 6904 bp
With 182 enzymes: SgfI *
February 27, 2009 14:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2344 4064 2344
Size: 1720 5184
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4714 278
Size: 53 83 186 936 3178 2468
Fragments arranged by size:
3178 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4247 1937
Size: 2310 4594
Acc65I G'GTAC_C
Cuts at: 4225 4225
Size: 6904
AclI AA'CG_TT
Cuts at: 1563 2876 5031 5404 1563
Size: 1313 2155 373 3063
Fragments arranged by size:
3063 2155 1313 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2388 2877 2934 2942 3243 3419 3548 3561
Size: 756 489 57 8 301 176 129 13
Cuts at: 3561 4967 6351 6619 1632
Size: 1406 1384 268 1917
Fragments arranged by size:
1917 1406 1384 756 489 301 268 176
129 57 13 8
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6707
AflIII A'CryG_T
Cuts at: 1203 5803 6780 1203
Size: 4600 977 1327
Fragments arranged by size:
4600 1327 977
AgeI A'CCGG_T
Cuts at: 5735 5735
Size: 6904
AhdI GACnn_n'nnGTC
Cuts at: 5633 5633
Size: 6904
AleI CACnn'nnGTG
Cuts at: 1166 3794 1166
Size: 2628 4276
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6904
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2165 2197 2165
Size: 32 6872
AlwNI CAG_nnn'CTG
Cuts at: 3397 4647 6219 3397
Size: 1250 1572 4082
Fragments arranged by size:
4082 1572 1250
ApaLI G'TGCA_C
Cuts at: 4961 6117 4961
Size: 1156 5748
ApoI r'AATT_y
Cuts at: 1493 2151 2217 2771 3021 3950 4213 4439 4591
Size: 658 66 554 250 929 263 226 152
Cuts at: 4591 6700 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 929 658 554 263 250 226
152 66
AseI AT'TA_AT
Cuts at: 160 1032 5458 160
Size: 872 4426 1606
Fragments arranged by size:
4426 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6904
AvaI C'yCGr_G
Cuts at: 1870 1943 3196 4229 1870
Size: 73 1253 1033 4545
Fragments arranged by size:
4545 1253 1033 73
BamHI G'GATC_C
Cuts at: 4234 4601 4234
Size: 367 6537
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4069 4225 5680 618
Size: 325 217 627 2282 156 1455 1842
Fragments arranged by size:
2282 1842 1455 627 325 217 156
BanII G_rGCy'C
Cuts at: 1391 2395 3627 4223 1391
Size: 1004 1232 596 4072
Fragments arranged by size:
4072 1232 1004 596
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2250 3011 3360 3536 3564 3670 928
Size: 971 351 761 349 176 28 106 4162
Fragments arranged by size:
4162 971 761 351 349 176 106 28
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5095 5129 1903
Size: 34 3158 34 3678
Fragments arranged by size:
3678 3158 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4796 6006 4796
Size: 1210 5694
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6904
BfrBI ATG'CAT
Cuts at: 3163 3163
Size: 6904
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3659 5515 136
Size: 107 122 71 631 605 1987 1856 1525
Fragments arranged by size:
1987 1856 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4100 6899 4100
Size: 2799 4105
BlpI GC'TnA_GC
Cuts at: 4321 4321
Size: 6904
Bme1580I G_kGCm'C
Cuts at: 1790 3568 4965 6121 1790
Size: 1778 1397 1156 2573
Fragments arranged by size:
2573 1778 1397 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6904
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2621 4635 5593
Size: 416 53 83 399 24 1204 2014 958
Cuts at: 5593 442
Size: 1753
Fragments arranged by size:
2014 1753 1204 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6904
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3116 3116
Size: 6904
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2409 3086 3794 5564 1239
Size: 1170 677 708 1770 2579
Fragments arranged by size:
2579 1770 1170 708 677
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5029 5894 6192 5029
Size: 865 298 5741
Fragments arranged by size:
5741 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2666 5567 882
Size: 617 1167 2901 2219
Fragments arranged by size:
2901 2219 1167 617
BsaAI yAC'GTr
Cuts at: 493 1204 4695 493
Size: 711 3491 2702
Fragments arranged by size:
3491 2702 711
BsaBI GATnn'nnATC
Cuts at: 6898 6898
Size: 6904
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4711 5093 5728 275
Size: 53 83 186 936 3178 382 635 1451
Fragments arranged by size:
3178 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5337 5735 6009 6156 6567 5337
Size: 398 274 147 411 5674
Fragments arranged by size:
5674 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2452 2482 3347 3377 1178
Size: 30 165 30 1049 30 865 30 4705
Fragments arranged by size:
4705 1049 865 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3291 3459 1186
Size: 2105 168 4631
Fragments arranged by size:
4631 2105 168
BseYI C'CCAG_C
Cuts at: 3445 6107 6603 3445
Size: 2662 496 3746
Fragments arranged by size:
3746 2662 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2322 1163
Size: 573 586 5745
Fragments arranged by size:
5745 586 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4354 5115 6143 1540
Size: 275 186 2353 761 1028 2301
Fragments arranged by size:
2353 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2395 4965 5050 6121 2395
Size: 2570 85 1071 3178
Fragments arranged by size:
3178 2570 1071 85
BsmI GAATG_Cn'
Cuts at: 3814 4415 4508 3814
Size: 601 93 6210
Fragments arranged by size:
6210 601 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2395 3568 3627 4223 4965 5050 6121
Size: 399 605 1173 59 596 742 85 1071
Cuts at: 6121 1391
Size: 2174
Fragments arranged by size:
2174 1173 1071 742 605 596 399 85
59
BspEI T'CCGG_A
Cuts at: 6567 6567
Size: 6904
BspHI T'CATG_A
Cuts at: 3381 3429 4793 6523 3381
Size: 48 1364 1730 3762
Fragments arranged by size:
3762 1730 1364 48
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2344 4064 4259 844
Size: 1500 1720 195 3489
Fragments arranged by size:
3489 1720 1500 195
BsrBI CCG'CTC
Cuts at: 1394 1823 4791 1394
Size: 429 2968 3507
Fragments arranged by size:
3507 2968 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3735 3769 5399 5573 65
Size: 1183 2487 34 1630 174 1396
Fragments arranged by size:
2487 1630 1396 1183 174 34
BsrFI r'CCGG_y
Cuts at: 1700 1810 2486 5548 5735 1700
Size: 110 676 3062 187 2869
Fragments arranged by size:
3062 2869 676 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6904
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6904
BssSI C'ACGA_G
Cuts at: 4964 5976 4964
Size: 1012 5892
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6904
BstBI TT'CG_AA
Cuts at: 2223 5708 2223
Size: 3485 3419
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6904
BstXI CCAn_nnnn'nTGG
Cuts at: 2503 2503
Size: 6904
BstYI r'GATC_y
Cuts at: 1972 3828 4100 4234 4601 4986 5003 6455 6541
Size: 1856 272 134 367 385 17 1452 86
Cuts at: 6541 6553 6899 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1856 1452 385 367 346 272 134
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2502 513
Size: 552 1437 4915
Fragments arranged by size:
4915 1437 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6165
Fragments arranged by size:
6165 683 56
BtsI GCAGTG_nn'
Cuts at: 4420 5214 5234 4420
Size: 794 20 6090
Fragments arranged by size:
6090 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6904
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6869
DraI TTT'AAA
Cuts at: 2115 2127 2751 4208 2115
Size: 12 624 1457 4811
Fragments arranged by size:
4811 1457 624 12
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2533 5911 809
Size: 507 1217 3378 1802
Fragments arranged by size:
3378 1802 1217 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2580 4351 5240
Size: 54 1425 50 275 18 750 1771 889
Cuts at: 5240 6869 8
Size: 1629 43
Fragments arranged by size:
1771 1629 1425 889 750 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4351 1537
Size: 275 2539 4090
Fragments arranged by size:
4090 2539 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2133 4834 1353
Size: 780 2701 3423
Fragments arranged by size:
3423 2701 780
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5491 5865 6011 387
Size: 1064 138 3902 374 146 1280
Fragments arranged by size:
3902 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2393 2393
Size: 6904
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2388 2409 2877 2934 2942 3086 3243
Size: 393 756 21 468 57 8 144 157
Cuts at: 3243 3419 3548 3561 3794 4967 5564 6351 6619
Size: 176 129 13 233 1173 597 787 268
Cuts at: 6619 1239
Size: 1524
Fragments arranged by size:
1524 1173 787 756 597 468 393 268
233 176 157 144 129 57 21 13
8
EcoRI G'AATT_C
Cuts at: 3950 4213 4591 3950
Size: 263 378 6263
Fragments arranged by size:
6263 378 263
FspI TGC'GCA
Cuts at: 5410 5410
Size: 6904
HaeII r_GCGC'y
Cuts at: 1678 6051 1678
Size: 4373 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2046 2078 2452 2484 3347
Size: 32 135 32 474 32 374 32 863
Cuts at: 3347 3379 4085 4117 5545 5577 5619 5651 1373
Size: 32 706 32 1428 32 42 32 2626
Fragments arranged by size:
2626 1428 863 706 474 374 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4248 4494 669
Size: 988 281 2310 246 3079
Fragments arranged by size:
3079 2310 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2946 748
Size: 2198 4706
HpaI GTT'AAC
Cuts at: 4494 4494
Size: 6904
KpnI G_GTAC'C
Cuts at: 4229 4229
Size: 6904
MfeI C'AATT_G
Cuts at: 4503 4503
Size: 6904
MluI A'CGCG_T
Cuts at: 6780 6780
Size: 6904
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6018 6202 1305
Size: 4713 184 2007
Fragments arranged by size:
4713 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2582 10
Size: 54 1425 343 750 4332
Fragments arranged by size:
4332 1425 750 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2501 3386 3794 4862 5221 5380 518
Size: 648 1335 885 408 1068 359 159 2042
Fragments arranged by size:
2042 1335 1068 885 648 408 359 159
MspA1I CmG'CkG
Cuts at: 1606 3449 4320 4997 6145 6390 1606
Size: 1843 871 677 1148 245 2120
Fragments arranged by size:
2120 1843 1148 871 677 245
NaeI GCC'GGC
Cuts at: 2488 2488
Size: 6904
NcoI C'CATG_G
Cuts at: 513 1065 2502 513
Size: 552 1437 4915
Fragments arranged by size:
4915 1437 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6904
NgoMIV G'CCGG_C
Cuts at: 2486 2486
Size: 6904
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6904
NotI GC'GGCC_GC
Cuts at: 4351 4351
Size: 6904
NsiI A_TGCA'T
Cuts at: 3165 3165
Size: 6904
NspI r_CATG'y
Cuts at: 1132 1229 2494 4262 5807 1132
Size: 97 1265 1768 1545 2229
Fragments arranged by size:
2229 1768 1545 1265 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6904
PciI A'CATG_T
Cuts at: 5803 5803
Size: 6904
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6904
PfoI T'CCnGG_A
Cuts at: 2386 3418 3771 2386
Size: 1032 353 5519
Fragments arranged by size:
5519 1032 353
PmeI GTTT'AAAC
Cuts at: 4208 4208
Size: 6904
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4973 5005 6486 6518 4973
Size: 32 1481 32 5359
Fragments arranged by size:
5359 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6904
PsiI TTA'TAA
Cuts at: 4474 4474
Size: 6904
PstI C_TGCA'G
Cuts at: 830 2482 4256 830
Size: 1652 1774 3478
Fragments arranged by size:
3478 1774 1652
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6904
PvuII CAG'CTG
Cuts at: 3449 3449
Size: 6904
SacI G_AGCT'C
Cuts at: 2395 2395
Size: 6904
SalI G'TCGA_C
Cuts at: 1936 4246 1936
Size: 2310 4594
SbfI CC_TGCA'GG
Cuts at: 2482 4256 2482
Size: 1774 5130
ScaI AGT'ACT
Cuts at: 5152 6864 5152
Size: 1712 5192
SfcI C'TryA_G
Cuts at: 826 1045 2478 2709 4252 5387 6068 6259 826
Size: 219 1433 231 1543 1135 681 191 1471
Fragments arranged by size:
1543 1471 1433 1135 681 231 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6904
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6904
SmaI CCC'GGG
Cuts at: 1872 4231 1872
Size: 2359 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5008 5909 6171 820
Size: 197 926 3065 901 262 1553
Fragments arranged by size:
3065 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4695 493
Size: 4202 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6904
SphI G_CATG'C
Cuts at: 1132 4262 1132
Size: 3130 3774
SspI AAT'ATT
Cuts at: 5 52 2959 3700 4828 5
Size: 47 2907 741 1128 2081
Fragments arranged by size:
2907 2081 1128 741 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6904
StyI C'CwwG_G
Cuts at: 513 1065 2502 4343 513
Size: 552 1437 1841 3074
Fragments arranged by size:
3074 1841 1437 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2560 3831 4931 4948 5101 5286 5731
Size: 1255 980 1271 1100 17 153 185 445
Cuts at: 5731 325
Size: 1498
Fragments arranged by size:
1498 1271 1255 1100 980 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2648 5150 6862
Size: 274 80 33 51 1443 671 2502 1712
Cuts at: 6862 96
Size: 138
Fragments arranged by size:
2502 1712 1443 671 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2718 2750 3891 3923 6864 6896 2718
Size: 32 1141 32 2941 32 2726
Fragments arranged by size:
2941 2726 1141 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3066 1316
Size: 1750 5154
XbaI T'CTAG_A
Cuts at: 4240 4240
Size: 6904
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6904
XmaI C'CCGG_G
Cuts at: 1870 4229 1870
Size: 2359 4545
XmnI GAAnn'nnTTC
Cuts at: 3377 5033 3377
Size: 1656 5248
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4712 276
Size: 53 83 186 936 3178 2468
Fragments arranged by size:
3178 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SacI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV
FalI FseI FspAI KasI NarI NruI PacI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI