Restriction map for
pFN21AB3482
(Linear) (Six-base) MAPSORT of: KIBB3482.seq Check: 8984 from: 1 to: 2199
KIBB3482.orf
With 182 enzymes: SgfI *
July 18, 2007 13:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 339 2059 2199
Size: 339 1720 140
Fragments arranged by size:
1720 339 140
AclI AA'CG_TT
Cuts at: 0 871 2199
Size: 871 1328
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 383 872 929 937 1238 1414 1543 1556
Size: 383 489 57 8 301 176 129 13
Cuts at: 1556 2199
Size: 643
Fragments arranged by size:
643 489 383 301 176 129 57 13
8
AleI CACnn'nnGTG
Cuts at: 0 1789 2199
Size: 1789 410
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 160 192 2199
Size: 160 32 2007
Fragments arranged by size:
2007 160 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1392 2199
Size: 1392 807
ApoI r'AATT_y
Cuts at: 0 146 212 766 1016 1945 2199
Size: 146 66 554 250 929 254
Fragments arranged by size:
929 554 254 250 146 66
AvaI C'yCGr_G
Cuts at: 0 1191 2199
Size: 1191 1008
BanI G'GyrC_C
Cuts at: 0 2064 2199
Size: 2064 135
BanII G_rGCy'C
Cuts at: 0 390 1622 2199
Size: 390 1232 577
Fragments arranged by size:
1232 577 390
BbsI GAAGACnn'nnnn_
Cuts at: 0 245 1006 1355 1531 1559 1665 2199
Size: 245 761 349 176 28 106 534
Fragments arranged by size:
761 534 349 245 176 106 28
BfrBI ATG'CAT
Cuts at: 0 1158 2199
Size: 1158 1041
BglI GCCn_nnn'nGGC
Cuts at: 0 1654 2199
Size: 1654 545
BglII A'GATC_T
Cuts at: 0 2095 2199
Size: 2095 104
Bme1580I G_kGCm'C
Cuts at: 0 1563 2199
Size: 1563 636
BmrI ACTGGGnnnn_n'
Cuts at: 0 616 2199
Size: 616 1583
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1111 2199
Size: 1111 1088
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 404 1081 1789 2199
Size: 404 677 708 410
Fragments arranged by size:
708 677 410 404
BsaI GGTCTCn'nnnn_
Cuts at: 0 661 2199
Size: 661 1538
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 447 477 1342 1372 2199
Size: 447 30 865 30 827
Fragments arranged by size:
865 827 447 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1286 1454 2199
Size: 1286 168 745
Fragments arranged by size:
1286 745 168
BseYI C'CCAG_C
Cuts at: 0 1440 2199
Size: 1440 759
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 317 2199
Size: 317 1882
BsiHKAI G_wGCw'C
Cuts at: 0 390 2199
Size: 390 1809
BsmI GAATG_Cn'
Cuts at: 0 1809 2199
Size: 1809 390
Bsp1286I G_dGCh'C
Cuts at: 0 390 1563 1622 2199
Size: 390 1173 59 577
Fragments arranged by size:
1173 577 390 59
BspHI T'CATG_A
Cuts at: 0 1376 1424 2199
Size: 1376 48 775
Fragments arranged by size:
1376 775 48
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 339 2059 2199
Size: 339 1720 140
Fragments arranged by size:
1720 339 140
BsrDI GCAATG_nn'
Cuts at: 0 1730 1764 2199
Size: 1730 34 435
Fragments arranged by size:
1730 435 34
BsrFI r'CCGG_y
Cuts at: 0 481 2199
Size: 481 1718
BstBI TT'CG_AA
Cuts at: 0 218 2199
Size: 218 1981
BstXI CCAn_nnnn'nTGG
Cuts at: 0 498 2199
Size: 498 1701
BstYI r'GATC_y
Cuts at: 0 1823 2095 2199
Size: 1823 272 104
Fragments arranged by size:
1823 272 104
BtgI C'CryG_G
Cuts at: 0 497 2199
Size: 497 1702
DraI TTT'AAA
Cuts at: 0 110 122 746 2199
Size: 110 12 624 1453
Fragments arranged by size:
1453 624 110 12
DrdI GACnn_nn'nnGTC
Cuts at: 0 528 2199
Size: 528 1671
EaeI y'GGCC_r
Cuts at: 0 575 2199
Size: 575 1624
EarI CTCTTCn'nnn_
Cuts at: 0 128 2199
Size: 128 2071
EcoICRI GAG'CTC
Cuts at: 0 388 2199
Size: 388 1811
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 383 404 872 929 937 1081 1238 1414
Size: 383 21 468 57 8 144 157 176
Cuts at: 1414 1543 1556 1789 2199
Size: 129 13 233 410
Fragments arranged by size:
468 410 383 233 176 157 144 129
57 21 13 8
EcoRI G'AATT_C
Cuts at: 0 1945 2199
Size: 1945 254
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 41 73 447 479 1342 1374 2080 2112
Size: 41 32 374 32 863 32 706 32
Cuts at: 2112 2199
Size: 87
Fragments arranged by size:
863 706 374 87 41 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 941 2199
Size: 941 1258
MscI TGG'CCA
Cuts at: 0 577 2199
Size: 577 1622
MslI CAynn'nnrTG
Cuts at: 0 496 1381 1789 2199
Size: 496 885 408 410
Fragments arranged by size:
885 496 410 408
MspA1I CmG'CkG
Cuts at: 0 1444 2199
Size: 1444 755
NaeI GCC'GGC
Cuts at: 0 483 2199
Size: 483 1716
NcoI C'CATG_G
Cuts at: 0 497 2199
Size: 497 1702
NgoMIV G'CCGG_C
Cuts at: 0 481 2199
Size: 481 1718
NsiI A_TGCA'T
Cuts at: 0 1160 2199
Size: 1160 1039
NspI r_CATG'y
Cuts at: 0 489 2199
Size: 489 1710
PfoI T'CCnGG_A
Cuts at: 0 381 1413 1766 2199
Size: 381 1032 353 433
Fragments arranged by size:
1032 433 381 353
PstI C_TGCA'G
Cuts at: 0 477 2199
Size: 477 1722
PvuII CAG'CTG
Cuts at: 0 1444 2199
Size: 1444 755
SacI G_AGCT'C
Cuts at: 0 390 2199
Size: 390 1809
SbfI CC_TGCA'GG
Cuts at: 0 477 2199
Size: 477 1722
SfcI C'TryA_G
Cuts at: 0 473 704 2199
Size: 473 231 1495
Fragments arranged by size:
1495 473 231
SspI AAT'ATT
Cuts at: 0 954 1695 2199
Size: 954 741 504
Fragments arranged by size:
954 741 504
StyI C'CwwG_G
Cuts at: 0 497 2199
Size: 497 1702
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 555 1826 2199
Size: 555 1271 373
Fragments arranged by size:
1271 555 373
TatI w'GTAC_w
Cuts at: 0 643 2199
Size: 643 1556
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 713 745 1886 1918 2199
Size: 713 32 1141 32 281
Fragments arranged by size:
1141 713 281 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1061 2199
Size: 1061 1138
XmnI GAAnn'nnTTC
Cuts at: 0 1372 2199
Size: 1372 827
Enzymes that do cut:
AarI AclI AcuI AleI AloI AlwNI ApoI AvaI
BanI BanII BbsI BfrBI BglI BglII Bme1580I BmrI
BplI BpmI BsaI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrDI BsrFI BstBI BstXI
BstYI BtgI DraI DrdI EaeI EarI EcoICRI Eco57MI
EcoRI Hin4I HindIII MscI MslI MspA1I NaeI NcoI
NgoMIV NsiI NspI PfoI PstI PvuII SacI SbfI
SfcI SspI StyI TaqII TatI TstI Tth111I XmnI
Enzymes that do not cut:
AatII AccI Acc65I AfeI AflII AflIII AgeI AhdI
AlfI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbvCI BcgI BciVI BclI BlpI BmgBI
BmtI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BsiEI
BsiWI BsmBI BspEI BsrBI BsrGI BssHII BssSI BstAPI
BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraIII
EagI EciI EcoNI EcoO109I EcoRV FalI FseI FspI
FspAI HaeII HincII HpaI KasI KpnI MfeI MluI
MmeI NarI NdeI NheI NotI NruI PacI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI
SapI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI StuI SwaI XbaI
XcmI XhoI XmaI ZraI