(Circular) (Six-base) MAPSORT of: pFN21AB3423.seq Check: 2661 from: 1 to: 5707 pFN21AB3423 5707 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2593 2665 2593 Size: 72 5635 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3517 278 Size: 53 83 186 936 1981 2468 Fragments arranged by size: 2468 1981 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3050 1937 Size: 1113 4594 Acc65I G'GTAC_C Cuts at: 3028 3028 Size: 5707 AclI AA'CG_TT Cuts at: 1563 3834 4207 1563 Size: 2271 373 3063 Fragments arranged by size: 3063 2271 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3770 5154 5422 1632 Size: 2138 1384 268 1917 Fragments arranged by size: 2138 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5510 AflIII A'CryG_T Cuts at: 1203 4606 5583 1203 Size: 3403 977 1327 Fragments arranged by size: 3403 1327 977 AgeI A'CCGG_T Cuts at: 4538 4538 Size: 5707 AhdI GACnn_n'nnGTC Cuts at: 4436 4436 Size: 5707 AleI CACnn'nnGTG Cuts at: 1166 2573 1166 Size: 1407 4300 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2751 1184 Size: 1567 4140 AlwNI CAG_nnn'CTG Cuts at: 3450 5022 3450 Size: 1572 4135 ApaLI G'TGCA_C Cuts at: 3764 4920 3764 Size: 1156 4551 ApoI r'AATT_y Cuts at: 1493 2098 2324 3016 3242 3394 5503 1493 Size: 605 226 692 226 152 2109 1697 Fragments arranged by size: 2109 1697 692 605 226 226 152 AseI AT'TA_AT Cuts at: 160 1032 4261 160 Size: 872 3229 1606 Fragments arranged by size: 3229 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5707 AvaI C'yCGr_G Cuts at: 1870 1943 2076 3032 1870 Size: 73 133 956 4545 Fragments arranged by size: 4545 956 133 73 BamHI G'GATC_C Cuts at: 3037 3404 3037 Size: 367 5340 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3028 4483 618 Size: 325 217 627 1241 1455 1842 Fragments arranged by size: 1842 1455 1241 627 325 217 BanII G_rGCy'C Cuts at: 1391 3026 1391 Size: 1635 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4736 BbvCI CC'TCA_GC Cuts at: 2553 2553 Size: 5707 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3898 3932 1903 Size: 34 1961 34 3678 Fragments arranged by size: 3678 1961 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3599 4809 3599 Size: 1210 4497 BclI T'GATC_A Cuts at: 1548 2184 2329 1548 Size: 636 145 4926 Fragments arranged by size: 4926 636 145 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2645 4318 136 Size: 107 122 71 631 605 973 1673 1525 Fragments arranged by size: 1673 1525 973 631 605 122 107 71 BglII A'GATC_T Cuts at: 5702 5702 Size: 5707 BlpI GC'TnA_GC Cuts at: 2451 2622 2761 3124 2451 Size: 171 139 363 5034 Fragments arranged by size: 5034 363 171 139 Bme1580I G_kGCm'C Cuts at: 1790 3768 4924 1790 Size: 1978 1156 2573 Fragments arranged by size: 2573 1978 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5707 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3438 4396 442 Size: 416 53 83 399 24 2021 958 1753 Fragments arranged by size: 2021 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5707 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4367 1239 Size: 3128 2579 Bpu10I CC'TnA_GC Cuts at: 2553 2553 Size: 5707 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2769 3832 4697 4995 2769 Size: 1063 865 298 3481 Fragments arranged by size: 3481 1063 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4370 882 Size: 617 2871 2219 Fragments arranged by size: 2871 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3498 493 Size: 711 2294 2702 Fragments arranged by size: 2702 2294 711 BsaBI GATnn'nnATC Cuts at: 2231 2540 5701 2231 Size: 309 3161 2237 Fragments arranged by size: 3161 2237 309 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3514 3896 4531 275 Size: 53 83 186 936 1981 382 635 1451 Fragments arranged by size: 1981 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2094 4140 4538 4812 4959 5370 2094 Size: 2046 398 274 147 411 2431 Fragments arranged by size: 2431 2046 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2957 2987 1178 Size: 30 165 30 1554 30 3898 Fragments arranged by size: 3898 1554 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2408 1186 Size: 1222 4485 BseYI C'CCAG_C Cuts at: 2049 2766 2940 4910 5406 2049 Size: 717 174 1970 496 2350 Fragments arranged by size: 2350 1970 717 496 174 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2144 2211 2627 2747 2813 1163 Size: 573 408 67 416 120 66 4057 Fragments arranged by size: 4057 573 416 408 120 67 66 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3157 3918 4946 1540 Size: 275 186 1156 761 1028 2301 Fragments arranged by size: 2301 1156 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3768 3853 4924 3768 Size: 85 1071 4551 Fragments arranged by size: 4551 1071 85 BsmI GAATG_Cn' Cuts at: 2740 3218 3311 2740 Size: 478 93 5136 Fragments arranged by size: 5136 478 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3026 3768 3853 4924 1391 Size: 399 1236 742 85 1071 2174 Fragments arranged by size: 2174 1236 1071 742 399 85 BspEI T'CCGG_A Cuts at: 2094 5370 2094 Size: 3276 2431 BspHI T'CATG_A Cuts at: 3596 5326 3596 Size: 1730 3977 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2575 2593 2665 3062 844 Size: 1731 18 72 397 3489 Fragments arranged by size: 3489 1731 397 72 18 BsrBI CCG'CTC Cuts at: 1394 1823 3594 1394 Size: 429 1771 3507 Fragments arranged by size: 3507 1771 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4202 4376 65 Size: 1183 2954 174 1396 Fragments arranged by size: 2954 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2738 2999 4351 4538 1700 Size: 110 928 261 1352 187 2869 Fragments arranged by size: 2869 1352 928 261 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5707 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5707 BssSI C'ACGA_G Cuts at: 3767 4779 3767 Size: 1012 4695 BstAPI GCAn_nnn'nTGC Cuts at: 794 2680 794 Size: 1886 3821 BstBI TT'CG_AA Cuts at: 4511 4511 Size: 5707 BstEII G'GTnAC_C Cuts at: 1184 2995 1184 Size: 1811 3896 BstYI r'GATC_y Cuts at: 1972 2709 2718 2802 2901 3037 3404 3789 3806 Size: 737 9 84 99 136 367 385 17 Cuts at: 3806 5258 5344 5356 5702 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 737 385 367 346 136 99 86 84 17 12 9 BtgI C'CryG_G Cuts at: 513 1065 2310 513 Size: 552 1245 3910 Fragments arranged by size: 3910 1245 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2527 487 Size: 683 56 1301 3667 Fragments arranged by size: 3667 1301 683 56 BtsI GCAGTG_nn' Cuts at: 2047 3223 4017 4037 2047 Size: 1176 794 20 3717 Fragments arranged by size: 3717 1176 794 20 ClaI AT'CG_AT Cuts at: 1554 2349 1554 Size: 795 4912 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5672 DraI TTT'AAA Cuts at: 3011 3011 Size: 5707 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4714 809 Size: 507 3398 1802 Fragments arranged by size: 3398 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2740 3154 4043 Size: 54 1425 50 275 18 910 414 889 Cuts at: 4043 5672 8 Size: 1629 43 Fragments arranged by size: 1629 1425 910 889 414 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3154 1537 Size: 275 1342 4090 Fragments arranged by size: 4090 1342 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2253 3637 1353 Size: 900 1384 3423 Fragments arranged by size: 3423 1384 900 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4294 4668 4814 387 Size: 1064 138 2705 374 146 1280 Fragments arranged by size: 2705 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3770 4367 5154 5422 1239 Size: 393 2138 597 787 268 1524 Fragments arranged by size: 2138 1524 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2775 2775 Size: 5707 EcoO109I rG'GnC_Cy Cuts at: 2932 2986 2932 Size: 54 5653 EcoRI G'AATT_C Cuts at: 3016 3394 3016 Size: 378 5329 EcoRV GAT'ATC Cuts at: 2305 2305 Size: 5707 FspI TGC'GCA Cuts at: 4213 4213 Size: 5707 HaeII r_GCGC'y Cuts at: 1678 2201 2284 4854 1678 Size: 523 83 2570 2531 Fragments arranged by size: 2570 2531 523 83 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4348 4380 4422 4454 1373 Size: 32 135 32 2776 32 42 32 2626 Fragments arranged by size: 2776 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2244 3051 3297 669 Size: 988 281 306 807 246 3079 Fragments arranged by size: 3079 988 807 306 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5707 HpaI GTT'AAC Cuts at: 3297 3297 Size: 5707 KpnI G_GTAC'C Cuts at: 3032 3032 Size: 5707 MfeI C'AATT_G Cuts at: 3306 3306 Size: 5707 MluI A'CGCG_T Cuts at: 5583 5583 Size: 5707 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2569 3000 4821 5005 1305 Size: 1264 431 1821 184 2007 Fragments arranged by size: 2007 1821 1264 431 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3885 Fragments arranged by size: 3885 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2573 2675 3665 4024 4183 518 Size: 648 1407 102 990 359 159 2042 Fragments arranged by size: 2042 1407 990 648 359 159 102 MspA1I CmG'CkG Cuts at: 1606 2465 2731 2746 3123 3800 4948 5193 1606 Size: 859 266 15 377 677 1148 245 2120 Fragments arranged by size: 2120 1148 859 677 377 266 245 15 NaeI GCC'GGC Cuts at: 2740 2740 Size: 5707 NcoI C'CATG_G Cuts at: 513 1065 2310 513 Size: 552 1245 3910 Fragments arranged by size: 3910 1245 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5707 NgoMIV G'CCGG_C Cuts at: 2738 2738 Size: 5707 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5707 NotI GC'GGCC_GC Cuts at: 3154 3154 Size: 5707 NspI r_CATG'y Cuts at: 1132 1229 3065 4610 1132 Size: 97 1836 1545 2229 Fragments arranged by size: 2229 1836 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5707 PciI A'CATG_T Cuts at: 4606 4606 Size: 5707 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5707 PmeI GTTT'AAAC Cuts at: 3011 3011 Size: 5707 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3776 3808 5289 5321 3776 Size: 32 1481 32 4162 Fragments arranged by size: 4162 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2932 2932 Size: 5707 PshAI GACnn'nnGTC Cuts at: 1613 2846 1613 Size: 1233 4474 PsiI TTA'TAA Cuts at: 3277 3277 Size: 5707 PstI C_TGCA'G Cuts at: 830 2751 3059 830 Size: 1921 308 3478 Fragments arranged by size: 3478 1921 308 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5707 PvuII CAG'CTG Cuts at: 2731 2746 2731 Size: 15 5692 SalI G'TCGA_C Cuts at: 1936 3049 1936 Size: 1113 4594 SbfI CC_TGCA'GG Cuts at: 3059 3059 Size: 5707 ScaI AGT'ACT Cuts at: 2435 3955 5667 2435 Size: 1520 1712 2475 Fragments arranged by size: 2475 1712 1520 SfcI C'TryA_G Cuts at: 826 1045 2060 2291 2747 3055 4190 4871 5062 Size: 219 1015 231 456 308 1135 681 191 Cuts at: 5062 826 Size: 1471 Fragments arranged by size: 1471 1135 1015 681 456 308 231 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5707 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5707 SmaI CCC'GGG Cuts at: 1872 3034 1872 Size: 1162 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2784 3811 4712 4974 820 Size: 197 926 841 1027 901 262 1553 Fragments arranged by size: 1553 1027 926 901 841 262 197 SnaBI TAC'GTA Cuts at: 493 3498 493 Size: 3005 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5707 SphI G_CATG'C Cuts at: 1132 3065 1132 Size: 1933 3774 SspI AAT'ATT Cuts at: 5 52 3631 5 Size: 47 3579 2081 Fragments arranged by size: 3579 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5707 StyI C'CwwG_G Cuts at: 513 1065 2214 2310 3146 513 Size: 552 1149 96 836 3074 Fragments arranged by size: 3074 1149 836 552 96 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3734 3751 3904 4089 4534 325 Size: 1255 2154 17 153 185 445 1498 Fragments arranged by size: 2154 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2203 2433 3953 Size: 274 80 33 51 1443 226 230 1520 Cuts at: 3953 5665 96 Size: 1712 138 Fragments arranged by size: 1712 1520 1443 274 230 226 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5667 5699 5667 Size: 32 5675 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5707 XbaI T'CTAG_A Cuts at: 3043 3043 Size: 5707 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2657 2882 2657 Size: 225 5482 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5707 XmaI C'CCGG_G Cuts at: 1870 3032 1870 Size: 1162 4545 XmnI GAAnn'nnTTC Cuts at: 2114 3836 2114 Size: 1722 3985 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3515 276 Size: 53 83 186 936 1981 2468 Fragments arranged by size: 2468 1981 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI