(Circular) (Six-base) MAPSORT of: pFN21AB3247.seq Check: 3887 from: 1 to: 5788 pFN21AB3247 5788 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2087 2087 Size: 5788 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3598 278 Size: 53 83 186 936 2062 2468 Fragments arranged by size: 2468 2062 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3131 1937 Size: 1194 4594 Acc65I G'GTAC_C Cuts at: 3109 3109 Size: 5788 AclI AA'CG_TT Cuts at: 1563 3915 4288 1563 Size: 2352 373 3063 Fragments arranged by size: 3063 2352 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2352 2400 2520 2556 3851 5235 5503 1632 Size: 720 48 120 36 1295 1384 268 1917 Fragments arranged by size: 1917 1384 1295 720 268 120 48 36 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5591 AflIII A'CryG_T Cuts at: 1203 4687 5664 1203 Size: 3484 977 1327 Fragments arranged by size: 3484 1327 977 AgeI A'CCGG_T Cuts at: 4619 4619 Size: 5788 AhdI GACnn_n'nnGTC Cuts at: 4517 4517 Size: 5788 AleI CACnn'nnGTG Cuts at: 1166 2312 2694 1166 Size: 1146 382 4260 Fragments arranged by size: 4260 1146 382 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5788 AlwNI CAG_nnn'CTG Cuts at: 2634 3531 5103 2634 Size: 897 1572 3319 Fragments arranged by size: 3319 1572 897 ApaLI G'TGCA_C Cuts at: 3845 5001 3845 Size: 1156 4632 ApoI r'AATT_y Cuts at: 1493 2799 3097 3323 3475 5584 1493 Size: 1306 298 226 152 2109 1697 Fragments arranged by size: 2109 1697 1306 298 226 152 AseI AT'TA_AT Cuts at: 160 1032 4342 160 Size: 872 3310 1606 Fragments arranged by size: 3310 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5788 AvaI C'yCGr_G Cuts at: 1870 1943 3113 1870 Size: 73 1170 4545 Fragments arranged by size: 4545 1170 73 BamHI G'GATC_C Cuts at: 2274 3118 3485 2274 Size: 844 367 4577 Fragments arranged by size: 4577 844 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2459 3109 4564 618 Size: 325 217 627 672 650 1455 1842 Fragments arranged by size: 1842 1455 672 650 627 325 217 BanII G_rGCy'C Cuts at: 1391 3107 1391 Size: 1716 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2508 2631 928 Size: 971 609 123 4085 Fragments arranged by size: 4085 971 609 123 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3979 4013 1903 Size: 34 2042 34 3678 Fragments arranged by size: 3678 2042 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3680 4890 3680 Size: 1210 4578 BclI T'GATC_A Cuts at: 1548 2814 2910 1548 Size: 1266 96 4426 Fragments arranged by size: 4426 1266 96 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4399 136 Size: 107 122 71 631 605 2727 1525 Fragments arranged by size: 2727 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5783 5783 Size: 5788 BlpI GC'TnA_GC Cuts at: 2054 3205 2054 Size: 1151 4637 Bme1580I G_kGCm'C Cuts at: 1790 2259 3849 5005 1790 Size: 469 1590 1156 2573 Fragments arranged by size: 2573 1590 1156 469 BmgBI CAC'GTC Cuts at: 1318 2536 1318 Size: 1218 4570 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2335 2688 3519 Size: 416 53 83 399 24 918 353 831 Cuts at: 3519 4477 442 Size: 958 1753 Fragments arranged by size: 1753 958 918 831 416 399 353 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5788 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2536 3006 4448 1239 Size: 1297 470 1442 2579 Fragments arranged by size: 2579 1442 1297 470 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3913 4778 5076 3913 Size: 865 298 4625 Fragments arranged by size: 4625 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2222 2667 4451 882 Size: 617 723 445 1784 2219 Fragments arranged by size: 2219 1784 723 617 445 BsaAI yAC'GTr Cuts at: 493 1204 2110 2260 3579 493 Size: 711 906 150 1319 2702 Fragments arranged by size: 2702 1319 906 711 150 BsaBI GATnn'nnATC Cuts at: 2725 5782 2725 Size: 3057 2731 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3595 3977 4612 275 Size: 53 83 186 936 2062 382 635 1451 Fragments arranged by size: 2062 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4221 4619 4893 5040 5451 4221 Size: 398 274 147 411 4558 Fragments arranged by size: 4558 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2270 2300 1178 Size: 30 165 30 867 30 4666 Fragments arranged by size: 4666 867 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2539 1186 Size: 1353 4435 BseYI C'CCAG_C Cuts at: 2513 4991 5487 2513 Size: 2478 496 2814 Fragments arranged by size: 2814 2478 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2316 1163 Size: 573 580 4635 Fragments arranged by size: 4635 580 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3238 3999 5027 1540 Size: 275 186 1237 761 1028 2301 Fragments arranged by size: 2301 1237 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3849 3934 5005 3849 Size: 85 1071 4632 Fragments arranged by size: 4632 1071 85 BsmI GAATG_Cn' Cuts at: 3299 3392 3299 Size: 93 5695 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2259 3107 3849 3934 5005 1391 Size: 399 469 848 742 85 1071 2174 Fragments arranged by size: 2174 1071 848 742 469 399 85 BspEI T'CCGG_A Cuts at: 5451 5451 Size: 5788 BspHI T'CATG_A Cuts at: 3677 5407 3677 Size: 1730 4058 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2087 2093 2226 2240 2415 3066 3143 844 Size: 1243 6 133 14 175 651 77 3489 Fragments arranged by size: 3489 1243 651 175 133 77 14 6 BsrBI CCG'CTC Cuts at: 1394 1823 3675 1394 Size: 429 1852 3507 Fragments arranged by size: 3507 1852 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2862 2929 4283 4457 65 Size: 1183 1614 67 1354 174 1396 Fragments arranged by size: 1614 1396 1354 1183 174 67 BsrFI r'CCGG_y Cuts at: 1700 1810 4432 4619 1700 Size: 110 2622 187 2869 Fragments arranged by size: 2869 2622 187 110 BsrGI T'GTAC_A Cuts at: 96 2316 2390 2605 96 Size: 2220 74 215 3279 Fragments arranged by size: 3279 2220 215 74 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5788 BssSI C'ACGA_G Cuts at: 2045 3848 4860 2045 Size: 1803 1012 2973 Fragments arranged by size: 2973 1803 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5788 BstBI TT'CG_AA Cuts at: 4592 4592 Size: 5788 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5788 BstXI CCAn_nnnn'nTGG Cuts at: 2696 2696 Size: 5788 BstYI r'GATC_y Cuts at: 1972 2274 2720 3118 3485 3870 3887 5339 5425 Size: 302 446 398 367 385 17 1452 86 Cuts at: 5425 5437 5783 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 446 398 385 367 346 302 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4297 Fragments arranged by size: 4297 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5049 Fragments arranged by size: 5049 683 56 BtsI GCAGTG_nn' Cuts at: 3304 4098 4118 3304 Size: 794 20 4974 Fragments arranged by size: 4974 794 20 ClaI AT'CG_AT Cuts at: 1554 2070 1554 Size: 516 5272 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5753 DraI TTT'AAA Cuts at: 3092 3092 Size: 5788 DraIII CAC_nnn'GTG Cuts at: 2263 2263 Size: 5788 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4795 809 Size: 507 3479 1802 Fragments arranged by size: 3479 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3235 4124 5753 Size: 54 1425 50 275 18 1405 889 1629 Cuts at: 5753 8 Size: 43 Fragments arranged by size: 1629 1425 1405 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3235 1537 Size: 275 1423 4090 Fragments arranged by size: 4090 1423 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3718 1353 Size: 2365 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4375 4749 4895 387 Size: 1064 138 2786 374 146 1280 Fragments arranged by size: 2786 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2352 2400 2520 2536 2556 3006 3851 Size: 393 720 48 120 16 20 450 845 Cuts at: 3851 4448 5235 5503 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 845 787 720 597 450 393 268 120 48 20 16 EcoNI CCTnn'n_nnAGG Cuts at: 2162 2162 Size: 5788 EcoO109I rG'GnC_Cy Cuts at: 2703 2703 Size: 5788 EcoRI G'AATT_C Cuts at: 3097 3475 3097 Size: 378 5410 FspI TGC'GCA Cuts at: 4294 4294 Size: 5788 HaeII r_GCGC'y Cuts at: 1678 4935 1678 Size: 3257 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2028 2060 2378 2410 2568 Size: 32 135 32 456 32 318 32 158 Cuts at: 2568 2600 2723 2755 4429 4461 4503 4535 1373 Size: 32 123 32 1674 32 42 32 2626 Fragments arranged by size: 2626 1674 456 318 158 135 123 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2449 2872 3132 3378 669 Size: 988 281 511 423 260 246 3079 Fragments arranged by size: 3079 988 511 423 281 260 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5788 HpaI GTT'AAC Cuts at: 2449 3378 2449 Size: 929 4859 KpnI G_GTAC'C Cuts at: 3113 3113 Size: 5788 MfeI C'AATT_G Cuts at: 3387 3387 Size: 5788 MluI A'CGCG_T Cuts at: 5664 5664 Size: 5788 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2355 4902 5086 1305 Size: 1050 2547 184 2007 Fragments arranged by size: 2547 2007 1050 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3966 Fragments arranged by size: 3966 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2312 2694 2742 3746 4105 4264 518 Size: 648 1146 382 48 1004 359 159 2042 Fragments arranged by size: 2042 1146 1004 648 382 359 159 48 MspA1I CmG'CkG Cuts at: 1606 2053 2153 2544 3041 3204 3881 5029 5274 Size: 447 100 391 497 163 677 1148 245 Cuts at: 5274 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 497 447 391 245 163 100 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4297 Fragments arranged by size: 4297 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5788 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5788 NotI GC'GGCC_GC Cuts at: 3235 3235 Size: 5788 NspI r_CATG'y Cuts at: 1132 1229 3146 4691 1132 Size: 97 1917 1545 2229 Fragments arranged by size: 2229 1917 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5788 PciI A'CATG_T Cuts at: 4687 4687 Size: 5788 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5788 PmeI GTTT'AAAC Cuts at: 3092 3092 Size: 5788 PmlI CAC'GTG Cuts at: 2110 2260 2110 Size: 150 5638 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3857 3889 5370 5402 3857 Size: 32 1481 32 4243 Fragments arranged by size: 4243 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2703 2703 Size: 5788 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5788 PsiI TTA'TAA Cuts at: 3358 3358 Size: 5788 PstI C_TGCA'G Cuts at: 830 2237 3063 3140 830 Size: 1407 826 77 3478 Fragments arranged by size: 3478 1407 826 77 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5788 PvuII CAG'CTG Cuts at: 3041 3041 Size: 5788 SalI G'TCGA_C Cuts at: 1936 3130 1936 Size: 1194 4594 SanDI GG'GwC_CC Cuts at: 2703 2703 Size: 5788 SbfI CC_TGCA'GG Cuts at: 2237 3140 2237 Size: 903 4885 ScaI AGT'ACT Cuts at: 4036 5748 4036 Size: 1712 4076 SfcI C'TryA_G Cuts at: 826 1045 2128 2233 3059 3136 4271 4952 5143 Size: 219 1083 105 826 77 1135 681 191 Cuts at: 5143 826 Size: 1471 Fragments arranged by size: 1471 1135 1083 826 681 219 191 105 77 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5788 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5788 SmaI CCC'GGG Cuts at: 1872 3115 1872 Size: 1243 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3892 4793 5055 820 Size: 197 926 1949 901 262 1553 Fragments arranged by size: 1949 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3579 493 Size: 3086 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5788 SphI G_CATG'C Cuts at: 1132 3146 1132 Size: 2014 3774 SspI AAT'ATT Cuts at: 5 52 3712 5 Size: 47 3660 2081 Fragments arranged by size: 3660 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5788 StyI C'CwwG_G Cuts at: 513 1065 2004 2838 3227 513 Size: 552 939 834 389 3074 Fragments arranged by size: 3074 939 834 552 389 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3815 3832 3985 4170 4615 325 Size: 1255 2235 17 153 185 445 1498 Fragments arranged by size: 2235 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2316 2390 2605 Size: 274 80 33 51 1443 339 74 215 Cuts at: 2605 4034 5746 96 Size: 1429 1712 138 Fragments arranged by size: 1712 1443 1429 339 274 215 138 80 74 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2270 2302 5748 5780 2270 Size: 32 3446 32 2278 Fragments arranged by size: 3446 2278 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5788 XbaI T'CTAG_A Cuts at: 3124 3124 Size: 5788 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2714 2714 Size: 5788 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5788 XmaI C'CCGG_G Cuts at: 1870 3113 1870 Size: 1243 4545 XmnI GAAnn'nnTTC Cuts at: 3917 3917 Size: 5788 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3596 276 Size: 53 83 186 936 2062 2468 Fragments arranged by size: 2468 2062 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI