Restriction map for
pFN21AB3244
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# NO CUT ENZYMES ( 46 enzymes) #
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AccIII AclI AgeI AocI ApaI
AscI AseI AsnI BbsI BpuAI
BsaWI BseAI BspEI BssHII Bst1107I
BstZ17I Bsu36I CpoI DraI EagI
EclXI Eco52I Eco81I EcoT22I FseI
HpaI MfeI MluI MroI MunI
NotI NruI NsiI PacI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SbfI SgrAI Sse8387I SwaI
XbaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AatII 5866 GACGTC GACGT^C
AflII 654 CTTAAG C^TTAAG
AhdI 3019 GACCTCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 2949 GAACCGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3019 GACCTCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 4128 CCTAGG C^CTAGG
BbeI 4387 GGCGCC GGCGC^C
BbrPI 4651 CACGTG CAC^GTG
BciVI 697 GTATCC GTATCC(6/5)
BfrI 654 CTTAAG C^TTAAG
BlnI 4128 CCTAGG C^CTAGG
BsaBI 2808 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 2307 CGACCG CG[GA][CT]^CG
BsiWI 3459 CGTACG C^GTACG
Bsp1407I 6443 TGTACA T^GTACA
BspHI 6323 TCATGA T^CATGA
BspLU11I 2762 ACATGT A^CATGT
BsrDI 3420 CATTGC GCAATG(2/0)
BsrGI 6443 TGTACA T^GTACA
BssSI 1729 CTCGTG CACGAG(-5/-1)
BstBI 2600 TTCGAA TT^CGAA
Eam1105I 3019 GACCTCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 6193 GAATTC G^AATTC
KasI 4387 GGCGCC G^GCGCC
KspI 6174 CCGCGG CCGC^GG
MamI 2808 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 4387 GGCGCC GG^CGCC
NdeI 4644 CATATG CA^TATG
NspV 2600 TTCGAA TT^CGAA
PmaCI 4651 CACGTG CAC^GTG
PmlI 4651 CACGTG CAC^GTG
RcaI 6323 TCATGA T^CATGA
SacII 6174 CCGCGG CCGC^GG
SalI 63 GTCGAC G^TCGAC
SapI 2088 GAAGAGC GCTCTTC(1/4)
ScaI 3661 AGTACT AGT^ACT
SexAI 4239 ACCAGGT A^CC[AT]GGT
SfoI 4387 GGCGCC GGC^GCC
SfuI 2600 TTCGAA TT^CGAA
SnaBI 1391 TACGTA TAC^GTA
SpeI 5290 ACTAGT A^CTAGT
SspBI 6443 TGTACA T^GTACA
SspI 1306 AATATT AAT^ATT
StuI 2132 AGGCCT AGG^CCT
XmnI 2949 GAACCGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
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AflIII 2762 ACATGT A^C[GA][CT]GT
3462 ACGTGT
Aor51HI 3134 AGCGCT AGC^GCT
4631 AGCGCT
AspI 1342 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5866 GACGTCGTC
AviII 2150 TGCGCA TGC^GCA
6137 TGCGCA
BamHI 530 GGATCC G^GATCC
6402 GGATCC
BcgI 571 CGATGCCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4851 CGACTGCTTTGC
BglII 143 AGATCT A^GATCT
2789 AGATCT
BspDI 4093 ATCGAT AT^CGAT
5194 ATCGAT
ClaI 4093 ATCGAT AT^CGAT
5194 ATCGAT
DraIII 2185 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
3497 CACTGGGTG
DrdI 22 GACGGGTCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4516 GACAGCGTGGTC
Eco47III 3134 AGCGCT AGC^GCT
4631 AGCGCT
FspI 2150 TGCGCA TGC^GCA
6137 TGCGCA
NheI 1935 GCTAGC G^CTAGC
3960 GCTAGC
PaeR7I 2824 CTCGAG C^TCGAG
3022 CTCGAG
PflFI 1342 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5866 GACGTCGTC
SfiI 1890 GGCCAGTAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
6159 GGCCTTCGTGGCC
SmaI 5217 CCCGGG CCC^GGG
6329 CCCGGG
SphI 3369 GCATGC GCATG^C
6140 GCATGC
Tth111I 1342 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5866 GACGTCGTC
XhoI 2824 CTCGAG C^TCGAG
3022 CTCGAG
XmaCI 5217 CCCGGG C^CCGGG
6329 CCCGGG
XmaI 5217 CCCGGG C^CCGGG
6329 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 144 enzymes) #
#########################################
Acc65I AccI AccII AciI AcsI
AcyI AfaI AluI Alw44I AlwI
AlwNI ApaLI ApoI Asp718I AspHI
AvaI AvaII BalI BanI BanII
BbvCI BbvI BclI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstAPI BstEII BstF5I BstNI BstPI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoO65I EcoRII EcoRV EcoT14I
FauI FbaI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HinfI
HpaII HphI ItaI KpnI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII