(Circular) (Six-base) MAPSORT of: pFN21AB3244.seq Check: 9350 from: 1 to: 11191 pFN21AB3244 11191 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 5279 6243 7047 7487 5279 Size: 964 804 440 8983 Fragments arranged by size: 8983 964 804 440 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7875 9001 278 Size: 53 83 186 936 6339 1126 2468 Fragments arranged by size: 6339 2468 1126 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2069 2361 3774 4641 8534 1937 Size: 132 292 1413 867 3893 4594 Fragments arranged by size: 4594 3893 1413 867 292 132 Acc65I G'GTAC_C Cuts at: 2479 2611 3651 5061 7216 8512 2479 Size: 132 1040 1410 2155 1296 5158 Fragments arranged by size: 5158 2155 1410 1296 1040 132 AclI AA'CG_TT Cuts at: 1563 9318 9691 1563 Size: 7755 373 3063 Fragments arranged by size: 7755 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2980 3018 3293 3806 4860 4953 6258 6500 Size: 1348 38 275 513 1054 93 1305 242 Cuts at: 6500 7715 8078 9254 10638 10906 1632 Size: 1215 363 1176 1384 268 1917 Fragments arranged by size: 1917 1384 1348 1305 1215 1176 1054 513 363 275 268 242 93 38 AfeI AGC'GCT Cuts at: 5141 6638 5141 Size: 1497 9694 AflII C'TTAA_G Cuts at: 820 1017 2659 820 Size: 197 1642 9352 Fragments arranged by size: 9352 1642 197 AflIII A'CryG_T Cuts at: 1203 4767 5467 10090 11067 1203 Size: 3564 700 4623 977 1327 Fragments arranged by size: 4623 3564 1327 977 700 AgeI A'CCGG_T Cuts at: 10022 10022 Size: 11191 AhdI GACnn_n'nnGTC Cuts at: 5029 9920 5029 Size: 4891 6300 AleI CACnn'nnGTG Cuts at: 1166 2298 3518 6311 7061 8045 8234 1166 Size: 1132 1220 2793 750 984 189 4123 Fragments arranged by size: 4123 2793 1220 1132 984 750 189 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3974 4076 5442 1184 Size: 2790 102 1366 6933 Fragments arranged by size: 6933 2790 1366 102 AlwNI CAG_nnn'CTG Cuts at: 2295 3340 3638 4495 6287 6379 6389 7213 7289 Size: 1045 298 857 1792 92 10 824 76 Cuts at: 7289 7525 7984 8934 10506 2295 Size: 236 459 950 1572 2980 Fragments arranged by size: 2980 1792 1572 1045 950 857 824 459 298 236 92 76 10 ApaLI G'TGCA_C Cuts at: 2975 6109 6230 6644 9248 10404 2975 Size: 3134 121 414 2604 1156 3762 Fragments arranged by size: 3762 3134 2604 1156 414 121 ApoI r'AATT_y Cuts at: 1493 3236 3610 4664 8198 8500 8726 8878 10987 Size: 1743 374 1054 3534 302 226 152 2109 Cuts at: 10987 1493 Size: 1697 Fragments arranged by size: 3534 2109 1743 1697 1054 374 302 226 152 AseI AT'TA_AT Cuts at: 160 1032 9745 160 Size: 872 8713 1606 Fragments arranged by size: 8713 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 11191 AvaI C'yCGr_G Cuts at: 1870 1943 2114 2239 2367 3247 3595 4829 5027 Size: 73 171 125 128 880 348 1234 198 Cuts at: 5027 7222 8334 8516 1870 Size: 2195 1112 182 4545 Fragments arranged by size: 4545 2195 1234 1112 880 348 198 182 171 128 125 73 AvrII C'CTAG_G Cuts at: 6133 6133 Size: 11191 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2595 2628 5053 5086 2595 Size: 33 2425 33 8700 Fragments arranged by size: 8700 2425 33 33 BamHI G'GATC_C Cuts at: 2535 8407 8521 8888 2535 Size: 5872 114 367 4838 Fragments arranged by size: 5872 4838 367 114 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2479 2525 2611 3651 4583 Size: 325 217 627 692 46 86 1040 932 Cuts at: 4583 4922 5061 5602 6392 6452 7157 7216 7475 Size: 339 139 541 790 60 705 59 259 Cuts at: 7475 7887 8128 8183 8512 9967 618 Size: 412 241 55 329 1455 1842 Fragments arranged by size: 1842 1455 1040 932 790 705 692 627 541 412 339 329 325 259 241 217 139 86 60 59 55 46 BanII G_rGCy'C Cuts at: 1391 2858 2932 3206 3254 3698 3726 3969 4302 Size: 1467 74 274 48 444 28 243 333 Cuts at: 4302 4830 5019 8510 1391 Size: 528 189 3491 4072 Fragments arranged by size: 4072 3491 1467 528 444 333 274 243 189 74 48 28 BbeI G_GCGC'C Cuts at: 6396 6396 Size: 11191 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 10220 BbvCI CC'TCA_GC Cuts at: 2598 7269 7282 7410 2598 Size: 4671 13 128 6379 Fragments arranged by size: 6379 4671 128 13 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2565 2599 6845 6879 9382 9416 1903 Size: 34 628 34 4246 34 2503 34 3678 Fragments arranged by size: 4246 3678 2503 628 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2713 9083 10293 2713 Size: 6370 1210 3611 Fragments arranged by size: 6370 3611 1210 BclI T'GATC_A Cuts at: 1548 3300 4186 7941 1548 Size: 1752 886 3755 4798 Fragments arranged by size: 4798 3755 1752 886 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2792 3569 Size: 107 122 71 631 605 337 783 777 Cuts at: 3569 3902 8171 9802 136 Size: 333 4269 1631 1525 Fragments arranged by size: 4269 1631 1525 783 777 631 605 337 333 122 107 71 BglII A'GATC_T Cuts at: 2148 4794 11186 2148 Size: 2646 6392 2153 Fragments arranged by size: 6392 2646 2153 BlpI GC'TnA_GC Cuts at: 5093 5726 6624 6983 7790 8608 5093 Size: 633 898 359 807 818 7676 Fragments arranged by size: 7676 898 818 807 633 359 Bme1580I G_kGCm'C Cuts at: 1790 2530 2979 3515 4586 4925 5939 6090 6113 Size: 740 449 536 1071 339 1014 151 23 Cuts at: 6113 6234 6455 6648 7160 9252 10408 1790 Size: 121 221 193 512 2092 1156 2573 Fragments arranged by size: 2573 2092 1156 1071 1014 740 536 512 449 339 221 193 151 121 23 BmgBI CAC'GTC Cuts at: 1318 5224 5641 1318 Size: 3906 417 6868 Fragments arranged by size: 6868 3906 417 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2688 2935 4505 Size: 416 53 83 399 24 1271 247 1570 Cuts at: 4505 5513 5679 8095 8922 9880 442 Size: 1008 166 2416 827 958 1753 Fragments arranged by size: 2416 1753 1570 1271 1008 958 827 416 399 247 166 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3944 5969 1056 Size: 2888 2025 6278 Fragments arranged by size: 6278 2888 2025 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2926 3742 6520 6826 7447 2926 Size: 816 2778 306 621 6670 Fragments arranged by size: 6670 2778 816 621 306 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2317 3252 5156 5372 5604 5840 5978 6785 Size: 1078 935 1904 216 232 236 138 807 Cuts at: 6785 7097 7920 9851 1239 Size: 312 823 1931 2579 Fragments arranged by size: 2579 1931 1904 1078 935 823 807 312 236 232 216 138 Bpu10I CC'TnA_GC Cuts at: 2598 2782 3205 3963 3968 4049 5147 5211 6749 Size: 184 423 758 5 81 1098 64 1538 Cuts at: 6749 6801 7269 7282 7410 2598 Size: 52 468 13 128 6379 Fragments arranged by size: 6379 1538 1098 758 468 423 184 128 81 64 52 13 5 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2543 5699 9316 10181 10479 2543 Size: 3156 3617 865 298 3255 Fragments arranged by size: 3617 3255 3156 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2222 2406 2494 2657 3518 5124 6491 Size: 617 723 184 88 163 861 1606 1367 Cuts at: 6491 6932 9854 882 Size: 441 2922 2219 Fragments arranged by size: 2922 2219 1606 1367 861 723 617 441 184 163 88 BsaAI yAC'GTr Cuts at: 493 1204 2251 3398 3846 5468 6658 8125 8982 Size: 711 1047 1147 448 1622 1190 1467 857 Cuts at: 8982 493 Size: 2702 Fragments arranged by size: 2702 1622 1467 1190 1147 1047 857 711 448 BsaBI GATnn'nnATC Cuts at: 4817 11185 4817 Size: 6368 4823 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3825 4638 5883 6025 Size: 53 83 186 936 2292 813 1245 142 Cuts at: 6025 6351 6393 7872 8998 9380 10015 275 Size: 326 42 1479 1126 382 635 1451 Fragments arranged by size: 2292 1479 1451 1245 1126 936 813 635 382 326 186 142 83 53 42 BsaWI w'CCGG_w Cuts at: 9624 10022 10296 10443 10854 9624 Size: 398 274 147 411 9961 Fragments arranged by size: 9961 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2015 2045 2669 2699 3573 Size: 30 165 30 612 30 624 30 874 Cuts at: 3573 3603 3791 3821 5586 5616 6884 6914 7070 Size: 30 188 30 1765 30 1268 30 156 Cuts at: 7070 7100 7770 7800 1178 Size: 30 670 30 4569 Fragments arranged by size: 4569 1765 1268 874 670 624 612 188 165 156 30 30 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2922 3734 4148 5930 6686 6908 7427 8024 Size: 1736 812 414 1782 756 222 519 597 Cuts at: 8024 8228 1186 Size: 204 4149 Fragments arranged by size: 4149 1782 1736 812 756 597 519 414 222 204 BseYI C'CCAG_C Cuts at: 2288 2407 3089 3646 3742 4007 4356 4918 6014 Size: 119 682 557 96 265 349 562 1096 Cuts at: 6014 6296 7097 7774 7893 7949 8014 8135 8479 Size: 282 801 677 119 56 65 121 344 Cuts at: 8479 10394 10890 2288 Size: 1915 496 2589 Fragments arranged by size: 2589 1915 1096 801 682 677 562 557 496 349 344 282 265 121 119 119 96 65 56 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3344 3690 4310 7529 7553 7688 7868 Size: 573 1608 346 620 3219 24 135 180 Cuts at: 7868 1163 Size: 4486 Fragments arranged by size: 4486 3219 1608 620 573 346 180 135 24 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4315 8641 9402 10430 1540 Size: 275 186 2314 4326 761 1028 2301 Fragments arranged by size: 4326 2314 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2160 2979 3021 3726 3969 4302 4463 4830 5019 Size: 819 42 705 243 333 161 367 189 Cuts at: 5019 6113 6234 6648 7736 7791 7884 7971 8241 Size: 1094 121 414 1088 55 93 87 270 Cuts at: 8241 9252 9337 10408 2160 Size: 1011 85 1071 2943 Fragments arranged by size: 2943 1094 1088 1071 1011 819 705 414 367 333 270 243 189 161 121 93 87 85 55 42 BsiWI C'GTAC_G Cuts at: 5464 5464 Size: 11191 BsmI GAATG_Cn' Cuts at: 3871 5290 5296 5462 8702 8795 3871 Size: 1419 6 166 3240 93 6267 Fragments arranged by size: 6267 3240 1419 166 93 6 BsmBI CGTCTCn'nnnn_ Cuts at: 3816 5170 6032 3816 Size: 1354 862 8975 Fragments arranged by size: 8975 1354 862 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2160 2530 2858 2932 2979 3021 3206 Size: 399 370 370 328 74 47 42 185 Cuts at: 3206 3254 3515 3698 3726 3969 4302 4463 4586 Size: 48 261 183 28 243 333 161 123 Cuts at: 4586 4830 4925 5019 5939 6090 6113 6234 6455 Size: 244 95 94 920 151 23 121 221 Cuts at: 6455 6648 7160 7736 7791 7884 7971 8241 8510 Size: 193 512 576 55 93 87 270 269 Cuts at: 8510 9252 9337 10408 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 920 742 576 512 399 370 370 333 328 270 269 261 244 243 221 193 185 183 161 151 123 121 95 94 93 87 85 74 55 48 47 42 28 23 BspEI T'CCGG_A Cuts at: 10854 10854 Size: 11191 BspHI T'CATG_A Cuts at: 8328 9080 10810 8328 Size: 752 1730 8709 Fragments arranged by size: 8709 1730 752 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4407 5279 6243 6728 7005 7047 7198 7487 Size: 3563 872 964 485 277 42 151 289 Cuts at: 7487 8546 844 Size: 1059 3489 Fragments arranged by size: 3563 3489 1059 964 872 485 289 277 151 42 BsrBI CCG'CTC Cuts at: 1394 1823 3121 5097 5356 7678 9078 1394 Size: 429 1298 1976 259 2322 1400 3507 Fragments arranged by size: 3507 2322 1976 1400 1298 429 259 BsrDI GCAATG_nn' Cuts at: 65 1248 5424 9686 9860 65 Size: 1183 4176 4262 174 1396 Fragments arranged by size: 4262 4176 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2096 5681 6021 6978 9835 10022 1700 Size: 110 286 3585 340 957 2857 187 2869 Fragments arranged by size: 3585 2869 2857 957 340 286 187 110 BsrGI T'GTAC_A Cuts at: 96 8448 96 Size: 8352 2839 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 11191 BssSI C'ACGA_G Cuts at: 3734 9251 10263 3734 Size: 5517 1012 4662 Fragments arranged by size: 5517 4662 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3677 7009 8368 794 Size: 2883 3332 1359 3617 Fragments arranged by size: 3617 3332 2883 1359 BstBI TT'CG_AA Cuts at: 4606 9995 4606 Size: 5389 5802 BstEII G'GTnAC_C Cuts at: 1184 2675 5317 8051 1184 Size: 1491 2642 2734 4324 Fragments arranged by size: 4324 2734 2642 1491 BstXI CCAn_nnnn'nTGG Cuts at: 2054 2888 2925 4882 5350 5503 8479 2054 Size: 834 37 1957 468 153 2976 4766 Fragments arranged by size: 4766 2976 1957 834 468 153 37 BstYI r'GATC_y Cuts at: 1972 2148 2535 2571 2754 4500 4794 5742 6675 Size: 176 387 36 183 1746 294 948 933 Cuts at: 6675 7072 8407 8521 8888 9273 9290 10742 10828 Size: 397 1335 114 367 385 17 1452 86 Cuts at: 10828 10840 11186 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1746 1452 1335 948 933 397 387 385 367 346 294 183 176 114 86 36 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2504 2887 4375 4857 4887 5055 Size: 552 939 500 383 1488 482 30 168 Cuts at: 5055 5181 5232 6309 6456 6600 7330 7764 8179 Size: 126 51 1077 147 144 730 434 415 Cuts at: 8179 513 Size: 3525 Fragments arranged by size: 3525 1488 1077 939 730 552 500 482 434 415 383 168 147 144 126 51 30 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4330 5245 487 Size: 683 56 3104 915 6433 Fragments arranged by size: 6433 3104 915 683 56 BtsI GCAGTG_nn' Cuts at: 2912 4518 4913 5337 7652 7911 7924 8707 9501 Size: 1606 395 424 2315 259 13 783 794 Cuts at: 9501 9521 2912 Size: 20 4582 Fragments arranged by size: 4582 2315 1606 794 783 424 395 259 20 13 ClaI AT'CG_AT Cuts at: 1554 6099 7200 1554 Size: 4545 1101 5545 Fragments arranged by size: 5545 4545 1101 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 11156 DraI TTT'AAA Cuts at: 8495 8495 Size: 11191 DraIII CAC_nnn'GTG Cuts at: 4195 5507 4195 Size: 1312 9879 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2033 6527 10198 809 Size: 507 717 4494 3671 1802 Fragments arranged by size: 4494 3671 1802 717 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3223 3342 3894 Size: 54 1425 50 275 18 1393 119 552 Cuts at: 3894 4544 4884 5526 5679 6069 6459 7250 7354 Size: 650 340 642 153 390 390 791 104 Cuts at: 7354 7914 8638 9527 11156 8 Size: 560 724 889 1629 43 Fragments arranged by size: 1629 1425 1393 889 791 724 650 642 560 552 390 390 340 275 153 119 104 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 8638 1537 Size: 275 6826 4090 Fragments arranged by size: 6826 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2660 2942 4088 6037 9121 1353 Size: 1307 282 1146 1949 3084 3423 Fragments arranged by size: 3423 3084 1949 1307 1146 282 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7154 8417 9778 10152 10298 387 Size: 1064 138 5565 1263 1361 374 146 1280 Fragments arranged by size: 5565 1361 1280 1263 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3724 3967 4300 4828 5017 3724 Size: 243 333 528 189 9898 Fragments arranged by size: 9898 528 333 243 189 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2317 2980 3018 3252 3293 3806 4860 Size: 393 685 663 38 234 41 513 1054 Cuts at: 4860 4953 5156 5372 5604 5840 5978 6258 6500 Size: 93 203 216 232 236 138 280 242 Cuts at: 6500 6785 7097 7715 7920 8078 9254 9851 10638 Size: 285 312 618 205 158 1176 597 787 Cuts at: 10638 10906 1239 Size: 268 1524 Fragments arranged by size: 1524 1176 1054 787 685 663 618 597 513 393 312 285 280 268 242 236 234 232 216 205 203 158 138 93 41 38 EcoNI CCTnn'n_nnAGG Cuts at: 3275 3712 7137 3275 Size: 437 3425 7329 Fragments arranged by size: 7329 3425 437 EcoO109I rG'GnC_Cy Cuts at: 2119 3211 3268 3505 4045 4150 5405 6130 6423 Size: 1092 57 237 540 105 1255 725 293 Cuts at: 6423 6776 6912 7530 7981 2119 Size: 353 136 618 451 5329 Fragments arranged by size: 5329 1255 1092 725 618 540 451 353 293 237 136 105 57 EcoRI G'AATT_C Cuts at: 8198 8500 8878 8198 Size: 302 378 10511 Fragments arranged by size: 10511 378 302 EcoRV GAT'ATC Cuts at: 3058 5335 6103 3058 Size: 2277 768 8146 Fragments arranged by size: 8146 2277 768 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 6283 6283 Size: 11191 FspI TGC'GCA Cuts at: 4157 8144 9697 4157 Size: 3987 1553 5651 Fragments arranged by size: 5651 3987 1553 HaeII r_GCGC'y Cuts at: 1678 2866 3136 5143 6396 6640 6772 8222 10338 Size: 1188 270 2007 1253 244 132 1450 2116 Cuts at: 10338 1678 Size: 2531 Fragments arranged by size: 2531 2116 2007 1450 1253 1188 270 244 132 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2015 2047 2669 2701 3142 Size: 32 135 32 443 32 622 32 441 Cuts at: 3142 3174 3529 3561 4781 4813 5015 5047 7610 Size: 32 355 32 1220 32 202 32 2563 Cuts at: 7610 7642 9832 9864 9906 9938 1373 Size: 32 2190 32 42 32 2626 Fragments arranged by size: 2626 2563 2190 1220 622 443 441 355 202 135 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2070 6175 8380 8535 8781 669 Size: 988 281 132 4105 2205 155 246 3079 Fragments arranged by size: 4105 3079 2205 988 281 246 155 132 HindIII A'AGCT_T Cuts at: 748 6194 6482 8292 748 Size: 5446 288 1810 3647 Fragments arranged by size: 5446 3647 1810 288 HpaI GTT'AAC Cuts at: 8781 8781 Size: 11191 KasI G'GCGC_C Cuts at: 6392 6392 Size: 11191 KpnI G_GTAC'C Cuts at: 2483 2615 3655 5065 7220 8516 2483 Size: 132 1040 1410 2155 1296 5158 Fragments arranged by size: 5158 2155 1410 1296 1040 132 MfeI C'AATT_G Cuts at: 8790 8790 Size: 11191 MluI A'CGCG_T Cuts at: 11067 11067 Size: 11191 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2001 4200 4253 7416 7942 10305 10489 1305 Size: 696 2199 53 3163 526 2363 184 2007 Fragments arranged by size: 3163 2363 2199 2007 696 526 184 53 MscI TGG'CCA Cuts at: 10 64 1489 1832 3225 3896 4546 4886 5528 Size: 54 1425 343 1393 671 650 340 642 Cuts at: 5528 7252 10 Size: 1724 3949 Fragments arranged by size: 3949 1724 1425 1393 671 650 642 343 340 54 MslI CAynn'nnrTG Cuts at: 518 1166 2298 3518 3860 4727 4886 5301 6311 Size: 648 1132 1220 342 867 159 415 1010 Cuts at: 6311 7061 8045 8234 8456 9149 9508 9667 518 Size: 750 984 189 222 693 359 159 2042 Fragments arranged by size: 2042 1220 1132 1010 984 867 750 693 648 415 359 342 222 189 159 159 MspA1I CmG'CkG Cuts at: 1606 3089 3638 4259 4317 4360 4538 4870 5443 Size: 1483 549 621 58 43 178 332 573 Cuts at: 5443 5700 5861 5897 5947 6074 6287 7409 8181 Size: 257 161 36 50 127 213 1122 772 Cuts at: 8181 8607 9284 10432 10677 1606 Size: 426 677 1148 245 2120 Fragments arranged by size: 2120 1483 1148 1122 772 677 621 573 549 426 332 257 245 213 178 161 127 58 50 43 36 NaeI GCC'GGC Cuts at: 5683 6023 6980 5683 Size: 340 957 9894 Fragments arranged by size: 9894 957 340 NarI GG'CG_CC Cuts at: 6393 6393 Size: 11191 NcoI C'CATG_G Cuts at: 513 1065 2004 2887 4857 4887 6309 7764 513 Size: 552 939 883 1970 30 1422 1455 3940 Fragments arranged by size: 3940 1970 1455 1422 939 883 552 30 NdeI CA'TA_TG Cuts at: 387 6650 387 Size: 6263 4928 NgoMIV G'CCGG_C Cuts at: 5681 6021 6978 5681 Size: 340 957 9894 Fragments arranged by size: 9894 957 340 NheI G'CTAG_C Cuts at: 1052 3940 5965 1052 Size: 2888 2025 6278 Fragments arranged by size: 6278 2888 2025 NotI GC'GGCC_GC Cuts at: 8638 8638 Size: 11191 NspI r_CATG'y Cuts at: 1132 1229 3590 4355 4771 5279 5378 6173 7108 Size: 97 2361 765 416 508 99 795 935 Cuts at: 7108 8149 8449 8549 10094 1132 Size: 1041 300 100 1545 2229 Fragments arranged by size: 2361 2229 1545 1041 935 795 765 508 416 300 100 99 97 PasI CC'CwG_GG Cuts at: 1348 2616 3098 3146 3334 3682 6929 7340 1348 Size: 1268 482 48 188 348 3247 411 5199 Fragments arranged by size: 5199 3247 1268 482 411 348 188 48 PciI A'CATG_T Cuts at: 4767 10090 4767 Size: 5323 5868 PflMI CCAn_nnn'nTGG Cuts at: 1108 2295 2776 3340 5056 6379 6538 6823 6889 Size: 1187 481 564 1716 1323 159 285 66 Cuts at: 6889 7525 1108 Size: 636 4774 Fragments arranged by size: 4774 1716 1323 1187 636 564 481 285 159 66 PfoI T'CCnGG_A Cuts at: 5202 5955 5202 Size: 753 10438 PmeI GTTT'AAAC Cuts at: 8495 8495 Size: 11191 PmlI CAC'GTG Cuts at: 6658 6658 Size: 11191 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 9260 9292 10773 10805 9260 Size: 32 1481 32 9646 Fragments arranged by size: 9646 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3505 4045 5405 7530 3505 Size: 540 1360 2125 7166 Fragments arranged by size: 7166 2125 1360 540 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 11191 PsiI TTA'TAA Cuts at: 8761 8761 Size: 11191 PspXI vC'TCGA_Gb Cuts at: 5027 5027 Size: 11191 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 8432 8464 8432 Size: 32 11159 PstI C_TGCA'G Cuts at: 830 2331 5421 6472 7005 8543 830 Size: 1501 3090 1051 533 1538 3478 Fragments arranged by size: 3478 3090 1538 1501 1051 533 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 11191 PvuII CAG'CTG Cuts at: 3089 4259 4360 4538 4870 5443 5861 5897 7409 Size: 1170 101 178 332 573 418 36 1512 Cuts at: 7409 3089 Size: 6871 Fragments arranged by size: 6871 1512 1170 573 418 332 178 101 36 SacI G_AGCT'C Cuts at: 3726 3969 4302 4830 5019 3726 Size: 243 333 528 189 9898 Fragments arranged by size: 9898 528 333 243 189 SacII CC_GC'GG Cuts at: 8182 8182 Size: 11191 SalI G'TCGA_C Cuts at: 1936 2068 8533 1936 Size: 132 6465 4594 Fragments arranged by size: 6465 4594 132 SanDI GG'GwC_CC Cuts at: 7530 7530 Size: 11191 SapI GCTCTTCn'nnn_ Cuts at: 4088 4088 Size: 11191 SbfI CC_TGCA'GG Cuts at: 8543 8543 Size: 11191 ScaI AGT'ACT Cuts at: 5668 9439 11151 5668 Size: 3771 1712 5708 Fragments arranged by size: 5708 3771 1712 SexAI A'CCwGG_T Cuts at: 6244 6244 Size: 11191 SfcI C'TryA_G Cuts at: 826 1045 2327 4680 5393 5417 5833 6468 7001 Size: 219 1282 2353 713 24 416 635 533 Cuts at: 7001 8539 9674 10355 10546 826 Size: 1538 1135 681 191 1471 Fragments arranged by size: 2353 1538 1471 1282 1135 713 681 635 533 416 219 191 24 SfiI GGCCn_nnn'nGGCC Cuts at: 3902 8171 3902 Size: 4269 6922 SfoI GGC'GCC Cuts at: 6394 6394 Size: 11191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 11191 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 11191 SmaI CCC'GGG Cuts at: 1872 7224 8336 8518 1872 Size: 5352 1112 182 4545 Fragments arranged by size: 5352 4545 1112 182 SmlI C'TyrA_G Cuts at: 820 1017 1943 2558 2659 4829 5027 5714 9295 Size: 197 926 615 101 2170 198 687 3581 Cuts at: 9295 10196 10458 820 Size: 901 262 1553 Fragments arranged by size: 3581 2170 1553 926 901 687 615 262 198 197 101 SnaBI TAC'GTA Cuts at: 493 3398 8982 493 Size: 2905 5584 2702 Fragments arranged by size: 5584 2905 2702 SpeI A'CTAG_T Cuts at: 152 7295 152 Size: 7143 4048 SphI G_CATG'C Cuts at: 1132 5378 8149 8549 1132 Size: 4246 2771 400 3774 Fragments arranged by size: 4246 3774 2771 400 SspI AAT'ATT Cuts at: 5 52 3313 9115 5 Size: 47 3261 5802 2081 Fragments arranged by size: 5802 3261 2081 47 StuI AGG'CCT Cuts at: 1517 4139 1517 Size: 2622 8569 StyI C'CwwG_G Cuts at: 513 1065 2004 2887 3471 4627 4857 4887 5430 Size: 552 939 883 584 1156 230 30 543 Cuts at: 5430 5523 6045 6133 6309 6418 6447 6888 7116 Size: 93 522 88 176 109 29 441 228 Cuts at: 7116 7614 7764 8630 513 Size: 498 150 866 3074 Fragments arranged by size: 3074 1156 939 883 866 584 552 543 522 498 441 230 228 176 150 109 93 88 30 29 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2023 2204 3027 3554 3674 4604 4850 Size: 1255 443 181 823 527 120 930 246 Cuts at: 4850 5495 5639 7991 8039 8114 8167 9218 9235 Size: 645 144 2352 48 75 53 1051 17 Cuts at: 9235 9388 9573 10018 325 Size: 153 185 445 1498 Fragments arranged by size: 2352 1498 1255 1051 930 823 645 527 445 443 246 185 181 153 144 120 75 53 48 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5666 8074 8448 Size: 274 80 33 51 1443 3689 2408 374 Cuts at: 8448 9437 11149 96 Size: 989 1712 138 Fragments arranged by size: 3689 2408 1712 1443 989 374 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3573 3605 3898 3930 4602 4634 5586 5618 5918 Size: 32 293 32 672 32 952 32 300 Cuts at: 5918 5950 6818 6850 6884 6909 6916 6941 7070 Size: 32 868 32 34 25 7 25 129 Cuts at: 7070 7102 7770 7802 11151 11183 3573 Size: 32 668 32 3349 32 3581 Fragments arranged by size: 3581 3349 952 868 672 668 300 293 129 34 32 32 32 32 32 32 32 32 32 25 25 7 Tth111I GACn'n_nGTC Cuts at: 1316 3350 7874 1316 Size: 2034 4524 4633 Fragments arranged by size: 4633 4524 2034 XbaI T'CTAG_A Cuts at: 8527 8527 Size: 11191 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3943 6121 7771 8347 3943 Size: 2178 1650 576 6787 Fragments arranged by size: 6787 2178 1650 576 XhoI C'TCGA_G Cuts at: 1943 4829 5027 1943 Size: 2886 198 8107 Fragments arranged by size: 8107 2886 198 XmaI C'CCGG_G Cuts at: 1870 7222 8334 8516 1870 Size: 5352 1112 182 4545 Fragments arranged by size: 5352 4545 1112 182 XmnI GAAnn'nnTTC Cuts at: 4958 9320 4958 Size: 4362 6829 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7873 8999 276 Size: 53 83 186 936 6339 1126 2468 Fragments arranged by size: 6339 2468 1126 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI BfrBI BstZ17I Bsu36I FseI FspAI NruI NsiI PacI PspOMI RsrII SrfI SwaI