Restriction map for
pFN21AB3232
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# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII AclI AcyI AflIII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I AspEI
AviII AvrII BamHI BbeI BbrPI
BbsI BciVI BclI BlnI BpuAI
BsaAI BsaBI BsaHI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspLU11I
BsrFI BsrGI BssHII BstAPI BstBI
Cfr10I ClaI DraI EagI Eam1105I
EclXI Eco47III Eco52I EcoRI EcoRV
EcoT22I FbaI FseI FspI HgaI
Hin1I HincII HindII HpaI KasI
KspI MaeII MamI MfeI MluI
MunI NaeI NarI NcoI NdeI
NgoMIV NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI SacII
SalI SapI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI SspBI
SspI StuI SwaI XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 120 GTATAC GT^[AC][GT]AC
AccIII 1200 TCCGGA T^CCGGA
AcsI 2133 AAATTC [GA]^AATT[CT]
AflII 859 CTTAAG C^TTAAG
ApoI 2133 AAATTC [GA]^AATT[CT]
AspI 1120 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 489 CCTCAGC CCTCAGC(-5/-2)
BcgI 903 CGACAAGTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 859 CTTAAG C^TTAAG
BlpI 357 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 357 GCTGAGC GC^T[ACGT]AGC
BsaWI 1200 TCCGGA [AT]^CCGG[AT]
BseAI 1200 TCCGGA T^CCGGA
BspEI 1200 TCCGGA T^CCGGA
BspHI 581 TCATGA T^CATGA
BssSI 10 CTCGTG CACGAG(-5/-1)
Bst1107I 120 GTATAC GTA^TAC
BstEII 1635 GGTCACC G^GT[ACGT]ACC
BstPI 1635 GGTCACC G^GT[ACGT]ACC
BstXI 1706 CCACAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 120 GTATAC GTA^TAC
CelII 357 GCTGAGC GC^T[ACGT]AGC
CpoI 1220 CGGTCCG CG^G[AT]CCG
DraIII 1182 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1059 GACGGTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1468 GAAGAG CTCTTC(1/4)
EcoO65I 1635 GGTCACC G^GT[ACGT]ACC
EcoT14I 1947 CCTTGG C^C[AT][AT]GG
HaeII 1211 AGCGCC [GA]GCGC^[CT]
HindIII 1281 AAGCTT A^AGCTT
Ksp632I 1468 GAAGAG CTCTTC(1/4)
MboII 1468 GAAGA GAAGA(8/7)
MroI 1200 TCCGGA T^CCGGA
MseI 860 TTAA T^TAA
MslI 1485 CACCTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PflFI 1120 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1497 CCAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1066 GAGTC GAGTC(4/5)
PpuMI 1438 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 180 GACGGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 581 TCATGA T^CATGA
RsrII 1220 CGGTCCG CG^G[AT]CCG
SacI 1588 GAGCTC GAGCT^C
SbfI 1080 CCTGCAGG CCTGCA^GG
ScaI 1775 AGTACT AGT^ACT
Sse8387I 1080 CCTGCAGG CCTGCA^GG
StyI 1947 CCTTGG C^C[AT][AT]GG
TfiI 2070 GAATC G^A[AT]TC
Tru9I 860 TTAA T^TAA
Tsp509I 2134 AATT ^AATT
Tth111I 1120 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1497 CCAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
Acc65I 66 GGTACC G^GTACC
1418 GGTACC
AccII 142 CGCG CG^CG
985 CGCG
AocI 528 CCTGAGG CC^T[ACGT]AGG
1871 CCTTAGG
Asp718I 66 GGTACC G^GTACC
1418 GGTACC
AspHI 1547 GTGCTC G[AT]GC[AT]^C
1588 GAGCTC
BglI 469 GCCCCCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1897 GCCATCCTGGC
BglII 680 AGATCT A^GATCT
1361 AGATCT
BsiHKAI 1547 GTGCTC G[AT]GC[AT]^C
1588 GAGCTC
BsrBI 129 CCGCTC CCGCTC(-3/-3)
814 CCGCTC
BstUI 142 CGCG CG^CG
985 CGCG
Bsu36I 528 CCTGAGG CC^T[ACGT]AGG
1871 CCTTAGG
CfoI 986 GCGC GCG^C
1212 GCGC
DraII 475 AGGGCCT [GA]G^G[ACGT]CC[CT]
1438 GGGACCC
Eco81I 528 CCTGAGG CC^T[ACGT]AGG
1871 CCTTAGG
EcoNI 1080 CCTGCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1758 CCTCCTTCAGG
EcoO109I 475 AGGGCCT [GA]G^G[ACGT]CC[CT]
1438 GGGACCC
HhaI 986 GCGC GCG^C
1212 GCGC
HinP1I 986 GCGC G^CGC
1212 GCGC
HinfI 1066 GAGTC G^A[ACGT]TC
2070 GAATC
KpnI 66 GGTACC GGTAC^C
1418 GGTACC
MvnI 142 CGCG CG^CG
985 CGCG
NspI 75 GCATGT [GA]CATG^[CT]
848 ACATGC
PstI 226 CTGCAG CTGCA^G
1081 CTGCAG
SexAI 1502 ACCTGGT A^CC[AT]GGT
1669 ACCTGGT
SmaI 749 CCCGGG CCC^GGG
781 CCCGGG
SmlI 787 CTCAAG C^T[CT][GA]AG
859 CTTAAG
XmaCI 749 CCCGGG C^CCGGG
781 CCCGGG
XmaI 749 CCCGGG C^CCGGG
781 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaI AvaII BalI BanI BanII
BbvI BcnI BfaI BmyI BpmI
BsaI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DsaI EaeI EcoRII FauI
Fnu4HI FokI HaeIII HapII HpaII
HphI ItaI MaeI MaeIII MboI
MflI MluNI MnlI MscI MspA1I
MspI MvaI MwoI NciI NdeII
NheI NlaIII NlaIV PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
TaqI TseI Tsp45I TspRI TthHB8I
XcmI XhoII