(Circular) (Six-base) MAPSORT of: pFN21AB3232.seq Check: 2255 from: 1 to: 6844 pFN21AB3232 6844 bp With 182 enzymes: SgfI * February 27, 2009 14:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3012 3412 3012 Size: 400 6444 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4654 278 Size: 53 83 186 936 3118 2468 Fragments arranged by size: 3118 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2126 4187 1937 Size: 189 2061 4594 Fragments arranged by size: 4594 2061 189 Acc65I G'GTAC_C Cuts at: 2071 3423 4165 2071 Size: 1352 742 4750 Fragments arranged by size: 4750 1352 742 AclI AA'CG_TT Cuts at: 1563 4971 5344 1563 Size: 3408 373 3063 Fragments arranged by size: 3408 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3752 4907 6291 6559 1632 Size: 2120 1155 1384 268 1917 Fragments arranged by size: 2120 1917 1384 1155 268 AflII C'TTAA_G Cuts at: 820 1017 2864 820 Size: 197 1847 4800 Fragments arranged by size: 4800 1847 197 AflIII A'CryG_T Cuts at: 1203 5743 6720 1203 Size: 4540 977 1327 Fragments arranged by size: 4540 1327 977 AgeI A'CCGG_T Cuts at: 5675 5675 Size: 6844 AhdI GACnn_n'nnGTC Cuts at: 5573 5573 Size: 6844 AleI CACnn'nnGTG Cuts at: 1166 3494 1166 Size: 2328 4516 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2522 2693 3347 1184 Size: 1338 171 654 4681 Fragments arranged by size: 4681 1338 654 171 AlwNI CAG_nnn'CTG Cuts at: 2224 2362 2553 3508 3589 4587 6159 2224 Size: 138 191 955 81 998 1572 2909 Fragments arranged by size: 2909 1572 998 955 191 138 81 ApaLI G'TGCA_C Cuts at: 4901 6057 4901 Size: 1156 5688 ApoI r'AATT_y Cuts at: 1493 4138 4153 4379 4531 6640 1493 Size: 2645 15 226 152 2109 1697 Fragments arranged by size: 2645 2109 1697 226 152 15 AseI AT'TA_AT Cuts at: 160 1032 5398 160 Size: 872 4366 1606 Fragments arranged by size: 4366 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6844 AvaI C'yCGr_G Cuts at: 1870 1943 2328 2754 2786 2933 3362 3437 3732 Size: 73 385 426 32 147 429 75 295 Cuts at: 3732 4169 1870 Size: 437 4545 Fragments arranged by size: 4545 437 429 426 385 295 147 75 73 32 BamHI G'GATC_C Cuts at: 4174 4541 4174 Size: 367 6477 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2048 2071 2758 2782 3155 Size: 325 217 627 261 23 687 24 373 Cuts at: 3155 3235 3423 4165 5620 618 Size: 80 188 742 1455 1842 Fragments arranged by size: 1842 1455 742 687 627 373 325 261 217 188 80 24 23 BanII G_rGCy'C Cuts at: 1391 2793 3597 3897 4163 1391 Size: 1402 804 300 266 4072 Fragments arranged by size: 4072 1402 804 300 266 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5873 BbvCI CC'TCA_GC Cuts at: 2495 2495 Size: 6844 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2897 2931 5035 5069 1903 Size: 34 960 34 2104 34 3678 Fragments arranged by size: 3678 2104 960 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4736 5946 4736 Size: 1210 5634 BclI T'GATC_A Cuts at: 1548 1548 Size: 6844 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2480 3908 5455 Size: 107 122 71 631 605 808 1428 1547 Cuts at: 5455 136 Size: 1525 Fragments arranged by size: 1547 1525 1428 808 631 605 122 107 71 BglII A'GATC_T Cuts at: 2685 3366 6839 2685 Size: 681 3473 2690 Fragments arranged by size: 3473 2690 681 BlpI GC'TnA_GC Cuts at: 2363 4261 2363 Size: 1898 4946 Bme1580I G_kGCm'C Cuts at: 1790 2067 2763 2773 2787 3158 3240 3346 4033 Size: 277 696 10 14 371 82 106 687 Cuts at: 4033 4905 6061 1790 Size: 872 1156 2573 Fragments arranged by size: 2573 1156 872 696 687 371 277 106 82 14 10 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6844 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3382 3793 3817 Size: 416 53 83 399 24 1965 411 24 Cuts at: 3817 4575 5533 442 Size: 758 958 1753 Fragments arranged by size: 1965 1753 958 758 416 411 399 83 53 24 24 BmtI G_CTAG'C Cuts at: 1056 2036 2666 3944 1056 Size: 980 630 1278 3956 Fragments arranged by size: 3956 1278 980 630 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2746 2917 4011 2746 Size: 171 1094 5579 Fragments arranged by size: 5579 1094 171 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2420 2723 2741 2807 3197 3287 3366 3470 Size: 1181 303 18 66 390 90 79 104 Cuts at: 3470 3545 3611 3987 4045 5504 1239 Size: 75 66 376 58 1459 2579 Fragments arranged by size: 2579 1459 1181 390 376 303 104 90 79 75 66 66 58 18 Bpu10I CC'TnA_GC Cuts at: 2495 2495 Size: 6844 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2777 4969 5834 6132 2777 Size: 2192 865 298 3489 Fragments arranged by size: 3489 2192 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2905 3011 3104 3602 5507 882 Size: 617 1406 106 93 498 1905 2219 Fragments arranged by size: 2219 1905 1406 617 498 106 93 BsaAI yAC'GTr Cuts at: 493 1204 4635 493 Size: 711 3431 2702 Fragments arranged by size: 3431 2702 711 BsaBI GATnn'nnATC Cuts at: 6838 6838 Size: 6844 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4651 5033 5668 275 Size: 53 83 186 936 3118 382 635 1451 Fragments arranged by size: 3118 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3205 5277 5675 5949 6096 6507 3205 Size: 2072 398 274 147 411 3542 Fragments arranged by size: 3542 2072 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2507 2537 4103 4133 1178 Size: 30 165 30 1104 30 1566 30 3889 Fragments arranged by size: 3889 1566 1104 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2393 2906 3548 3679 1186 Size: 1207 513 642 131 4351 Fragments arranged by size: 4351 1207 642 513 131 BseYI C'CCAG_C Cuts at: 2269 2355 2872 3138 3386 3935 6047 6543 2269 Size: 86 517 266 248 549 2112 496 2570 Fragments arranged by size: 2570 2112 549 517 496 266 248 86 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2354 2498 2843 3623 1163 Size: 573 618 144 345 780 4384 Fragments arranged by size: 4384 780 618 573 345 144 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4294 5055 6083 1540 Size: 275 186 2293 761 1028 2301 Fragments arranged by size: 2301 2293 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3556 3597 4905 4990 6061 3556 Size: 41 1308 85 1071 4339 Fragments arranged by size: 4339 1308 1071 85 41 BsmI GAATG_Cn' Cuts at: 4355 4448 4355 Size: 93 6751 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2067 2763 2773 2787 2793 3158 3240 Size: 399 277 696 10 14 6 365 82 Cuts at: 3240 3346 3556 3597 3897 4033 4163 4905 4990 Size: 106 210 41 300 136 130 742 85 Cuts at: 4990 6061 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 696 399 365 300 277 210 136 130 106 85 82 41 14 10 6 BspEI T'CCGG_A Cuts at: 3205 6507 3205 Size: 3302 3542 BspHI T'CATG_A Cuts at: 2586 4733 6463 2586 Size: 2147 1730 2967 Fragments arranged by size: 2967 2147 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2655 2685 3012 3093 3412 4199 844 Size: 1811 30 327 81 319 787 3489 Fragments arranged by size: 3489 1811 787 327 319 81 30 BsrBI CCG'CTC Cuts at: 1394 1823 2136 2821 4731 1394 Size: 429 313 685 1910 3507 Fragments arranged by size: 3507 1910 685 429 313 BsrDI GCAATG_nn' Cuts at: 65 1248 2176 3334 3897 5339 5513 65 Size: 1183 928 1158 563 1442 174 1396 Fragments arranged by size: 1442 1396 1183 1158 928 563 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5488 5675 1700 Size: 110 3678 187 2869 Fragments arranged by size: 3678 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6844 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6844 BssSI C'ACGA_G Cuts at: 2015 4904 5916 2015 Size: 2889 1012 2943 Fragments arranged by size: 2943 2889 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6844 BstBI TT'CG_AA Cuts at: 5648 5648 Size: 6844 BstEII G'GTnAC_C Cuts at: 1184 3640 1184 Size: 2456 4388 BstXI CCAn_nnnn'nTGG Cuts at: 3718 3718 Size: 6844 BstYI r'GATC_y Cuts at: 1972 2112 2685 3366 4097 4174 4541 4926 4943 Size: 140 573 681 731 77 367 385 17 Cuts at: 4943 6395 6481 6493 6839 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 731 681 573 385 367 346 140 86 77 17 12 BstZ17I GTA'TAC Cuts at: 2127 2127 Size: 6844 Bsu36I CC'TnA_GG Cuts at: 2534 3877 2534 Size: 1343 5501 BtgI C'CryG_G Cuts at: 513 1065 2067 2966 3103 3150 3774 513 Size: 552 1002 899 137 47 624 3583 Fragments arranged by size: 3583 1002 899 624 552 137 47 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2455 487 Size: 683 56 1229 4876 Fragments arranged by size: 4876 1229 683 56 BtsI GCAGTG_nn' Cuts at: 2052 4360 5154 5174 2052 Size: 2308 794 20 3722 Fragments arranged by size: 3722 2308 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6844 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6809 DraI TTT'AAA Cuts at: 4148 4148 Size: 6844 DraIII CAC_nnn'GTG Cuts at: 3192 3192 Size: 6844 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3070 5851 809 Size: 507 1754 2781 1802 Fragments arranged by size: 2781 1802 1754 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2280 2424 2640 Size: 54 1425 50 275 18 450 144 216 Cuts at: 2640 3348 4291 5180 6809 8 Size: 708 943 889 1629 43 Fragments arranged by size: 1629 1425 943 889 708 450 275 216 144 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4291 1537 Size: 275 2479 4090 Fragments arranged by size: 4090 2479 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3468 4774 1353 Size: 2115 1306 3423 Fragments arranged by size: 3423 2115 1306 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5431 5805 5951 387 Size: 1064 138 3842 374 146 1280 Fragments arranged by size: 3842 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3595 3595 Size: 6844 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2420 2723 2741 2807 3197 3287 3366 Size: 393 788 303 18 66 390 90 79 Cuts at: 3366 3470 3545 3611 3752 3987 4045 4907 5504 Size: 104 75 66 141 235 58 862 597 Cuts at: 5504 6291 6559 1239 Size: 787 268 1524 Fragments arranged by size: 1524 862 788 787 597 393 390 303 268 235 141 104 90 79 75 66 66 58 18 EcoNI CCTnn'n_nnAGG Cuts at: 3089 3767 3089 Size: 678 6166 EcoO109I rG'GnC_Cy Cuts at: 2481 3444 2481 Size: 963 5881 EcoRI G'AATT_C Cuts at: 4153 4531 4153 Size: 378 6466 FspI TGC'GCA Cuts at: 5350 5350 Size: 6844 HaeII r_GCGC'y Cuts at: 1678 3220 5991 1678 Size: 1542 2771 2531 Fragments arranged by size: 2771 2531 1542 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3167 3199 5485 5517 5559 Size: 32 135 32 1595 32 2286 32 42 Cuts at: 5559 5591 1373 Size: 32 2626 Fragments arranged by size: 2626 2286 1595 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4188 4434 669 Size: 988 281 2250 246 3079 Fragments arranged by size: 3079 2250 988 281 246 HindIII A'AGCT_T Cuts at: 748 3286 748 Size: 2538 4306 HpaI GTT'AAC Cuts at: 4434 4434 Size: 6844 KpnI G_GTAC'C Cuts at: 2075 3427 4169 2075 Size: 1352 742 4750 Fragments arranged by size: 4750 1352 742 MfeI C'AATT_G Cuts at: 4443 4443 Size: 6844 MluI A'CGCG_T Cuts at: 6720 6720 Size: 6844 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2550 2715 5958 6142 1305 Size: 1245 165 3243 184 2007 Fragments arranged by size: 3243 2007 1245 184 165 MscI TGG'CCA Cuts at: 10 64 1489 1832 2282 2426 2642 10 Size: 54 1425 343 450 144 216 4212 Fragments arranged by size: 4212 1425 450 343 216 144 54 MslI CAynn'nnrTG Cuts at: 518 1166 3494 4802 5161 5320 518 Size: 648 2328 1308 359 159 2042 Fragments arranged by size: 2328 2042 1308 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2359 2389 2553 2749 2827 3120 3138 3386 Size: 753 30 164 196 78 293 18 248 Cuts at: 3386 3935 4260 4937 6085 6330 1606 Size: 549 325 677 1148 245 2120 Fragments arranged by size: 2120 1148 753 677 549 325 293 248 245 196 164 78 30 18 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6292 NdeI CA'TA_TG Cuts at: 387 387 Size: 6844 NheI G'CTAG_C Cuts at: 1052 2032 2662 3940 1052 Size: 980 630 1278 3956 Fragments arranged by size: 3956 1278 980 630 NotI GC'GGCC_GC Cuts at: 4291 4291 Size: 6844 NspI r_CATG'y Cuts at: 1132 1229 2084 2857 4202 5747 1132 Size: 97 855 773 1345 1545 2229 Fragments arranged by size: 2229 1545 1345 855 773 97 PasI CC'CwG_GG Cuts at: 1348 2478 1348 Size: 1130 5714 PciI A'CATG_T Cuts at: 5743 5743 Size: 6844 PflMI CCAn_nnn'nTGG Cuts at: 1108 3508 1108 Size: 2400 4444 PfoI T'CCnGG_A Cuts at: 2616 3243 2616 Size: 627 6217 PmeI GTTT'AAAC Cuts at: 4148 4148 Size: 6844 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2430 2462 4913 4945 6426 6458 2430 Size: 32 2451 32 1481 32 2816 Fragments arranged by size: 2816 2451 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3444 3444 Size: 6844 PshAI GACnn'nnGTC Cuts at: 1613 2189 1613 Size: 576 6268 PsiI TTA'TAA Cuts at: 4414 4414 Size: 6844 PstI C_TGCA'G Cuts at: 830 2235 3090 4196 830 Size: 1405 855 1106 3478 Fragments arranged by size: 3478 1405 1106 855 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6844 PvuII CAG'CTG Cuts at: 2359 2389 2749 3386 3935 2359 Size: 30 360 637 549 5268 Fragments arranged by size: 5268 637 549 360 30 RsrII CG'GwC_CG Cuts at: 3226 3226 Size: 6844 SacI G_AGCT'C Cuts at: 3597 3597 Size: 6844 SalI G'TCGA_C Cuts at: 1936 4186 1936 Size: 2250 4594 SanDI GG'GwC_CC Cuts at: 3444 3444 Size: 6844 SbfI CC_TGCA'GG Cuts at: 3090 4196 3090 Size: 1106 5738 ScaI AGT'ACT Cuts at: 3782 5092 6804 3782 Size: 1310 1712 3822 Fragments arranged by size: 3822 1712 1310 SexAI A'CCwGG_T Cuts at: 3507 3674 3507 Size: 167 6677 SfcI C'TryA_G Cuts at: 826 1045 2231 2432 3086 4192 5327 6008 6199 Size: 219 1186 201 654 1106 1135 681 191 Cuts at: 6199 826 Size: 1471 Fragments arranged by size: 1471 1186 1135 1106 681 654 219 201 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6844 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6844 SmaI CCC'GGG Cuts at: 1872 2756 2788 4171 1872 Size: 884 32 1383 4545 Fragments arranged by size: 4545 1383 884 32 SmlI C'TyrA_G Cuts at: 820 1017 1943 2792 2864 4948 5849 6111 820 Size: 197 926 849 72 2084 901 262 1553 Fragments arranged by size: 2084 1553 926 901 849 262 197 72 SnaBI TAC'GTA Cuts at: 493 4635 493 Size: 4142 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6844 SphI G_CATG'C Cuts at: 1132 4202 1132 Size: 3070 3774 SrfI GCCC'GGGC Cuts at: 2788 2788 Size: 6844 SspI AAT'ATT Cuts at: 5 52 4768 5 Size: 47 4716 2081 Fragments arranged by size: 4716 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6844 StyI C'CwwG_G Cuts at: 513 1065 3952 4283 513 Size: 552 2887 331 3074 Fragments arranged by size: 3074 2887 552 331 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2029 3664 3836 3971 4871 4888 5041 Size: 1255 449 1635 172 135 900 17 153 Cuts at: 5041 5226 5671 325 Size: 185 445 1498 Fragments arranged by size: 1635 1498 1255 900 449 445 185 172 153 135 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3780 5090 6802 Size: 274 80 33 51 1443 1803 1310 1712 Cuts at: 6802 96 Size: 138 Fragments arranged by size: 1803 1712 1443 1310 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2507 2539 3273 3305 6804 6836 2507 Size: 32 734 32 3499 32 2515 Fragments arranged by size: 3499 2515 734 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3128 1316 Size: 1812 5032 XbaI T'CTAG_A Cuts at: 4180 4180 Size: 6844 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2065 2770 3311 3976 2065 Size: 705 541 665 4933 Fragments arranged by size: 4933 705 665 541 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6844 XmaI C'CCGG_G Cuts at: 1870 2754 2786 4169 1870 Size: 884 32 1383 4545 Fragments arranged by size: 4545 1383 884 32 XmnI GAAnn'nnTTC Cuts at: 4973 4973 Size: 6844 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4652 276 Size: 53 83 186 936 3118 2468 Fragments arranged by size: 3118 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII RsrII SacI SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI SacII SapI SfiI SfoI SwaI